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See detailApoptosis and cytolysis induced by giganteosides and hederacolchisides in HL-60 cells
Gerkens, Pascal; Dobson, Rowan ULg; tabatadze, Nino et al

in Anticancer Research (2007), 27

The viability, cytolysis and apoptosis-mediated cellular death induced by giganteosides D and E (Gig-D and Gig-E) and hederacolchisides A and A1 (Hcol-A and Hcol- A1) were analysed in HL-60 cells ... [more ▼]

The viability, cytolysis and apoptosis-mediated cellular death induced by giganteosides D and E (Gig-D and Gig-E) and hederacolchisides A and A1 (Hcol-A and Hcol- A1) were analysed in HL-60 cells. Materials and Methods: the end-point metabolic (WST1) and lactate dehydrogenase (LDH) assays were used. Cell cycle analysis and apoptosis were measured by flow cytometry, DNA laddering and caspase 3 analyses. Results: the HL-60 cell line was more sensitive to Hcol-A1 and Gig-D (IC50 3-5 ÌM) than to Gig-E and Hcol-A (IC50 8-13 ÌM; WST1 assay). This was related to LDH release. The induction of apoptosis could be detected without caspase 3 activation after 24 h of treatment. DNA fragmentation could be detected only with Gig-D. With Hcol- A1 and Gig-D, an accumulation of cells in the S-phase and an increase of cells in sub-G1 peak were observed. By the annexinV-fluorescein isothiocyanate (FITC)/7-aminoactinomycin D (AAD) assay, the majority of cells were in late apoptosis with Gig-D, and in necrosis with Hcol-A1. Conclusion: Hcol-A1 is more cytotoxic than Gig-D, followed by Gig-E and finally Hcol-A. This is related to a membrane permeabilization effect, leading to cytolysis [less ▲]

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See detailCytotoxic triterpenoid saponins from the roots of Cephalaria gigantea
Tabatadze, Nino; Elias, Riad; Faure, Robert et al

in Chemical & Pharmaceutical Bulletin (2007), 55(1), 102-105

Three new oleanane-type saponins, giganteosides L (1)(I), M (2) and N (3) along with eight known ones were isolated from the roots of Cephalaria gigantea. Their structures were established as 3-O-[-D ... [more ▼]

Three new oleanane-type saponins, giganteosides L (1)(I), M (2) and N (3) along with eight known ones were isolated from the roots of Cephalaria gigantea. Their structures were established as 3-O-[-D-galactopyranosyl-(12)--D-glucuronopyranosyl]-28-O-[-D-glucopyranosyl-(16)--D-glucopyranosyl]-oleanolic acid, 3-O-[-D-galactopyranosyl-(12)--D-glucuronopyranosyl]-28-O-[-D-glucopyranosyl-(16)--D-glucopyranosyl]-hederagenin, 3-O-[-L-rhamnopyranosyl-(12)--D-glucuronopyranosyl]-28-O-[-D-glucopyranosyl-(16)--D-glucopyranosyl]-hederagenin, resp., by means of spectroscopic methods (1D and 2D NMR, HR-ESI-MS). Cytotoxic activity of monodesmosides was investigated in vitro using three cancer cell lines, namely, human non pigmented melanoma MEL-5 and human leukemia HL-60. Giganteosides D (4) and E (5) showed antiproliferative effect on human cell lines with IC50 values in the range 3.15-7.5 M. [less ▲]

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See detailAntiprotozoal and cytotoxic triterpenoid saponins from the roots of Cephalaria gigantea
Tabatadze, Nino; Elias, Riad; Delmas, F. et al

Poster (2006, July)

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