References of "Sulon, José"
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See detailAccuracy of transrectal palpation for early pregnancy diagnosis in Egyptian buffaloes.
Karen, A. M.; Darwish, S.; Ramoun, A. et al

in Tropical Animal Health & Production (2011), 43(1), 5-7

The present study was undertaken to evaluate the accuracy of transrectal palpation (TRP) for diagnosing early pregnancy in buffaloes and the false diagnoses of the TRP test by using the pregnancy ... [more ▼]

The present study was undertaken to evaluate the accuracy of transrectal palpation (TRP) for diagnosing early pregnancy in buffaloes and the false diagnoses of the TRP test by using the pregnancy-associated glycoprotein radioimmunoassay (PAG-RIA) test. Pregnancy was diagnosed in 168 buffalo-cows once by TRP and PAG-RIA test between days 31 and 55 after breeding. The sensitivity of TRP for detecting pregnant buffalo-cows was 37.5% at days 31-35, increased to 93.8% at days 46-50 and reached 100% at days 51-55 (P < 0.01). All cases of false negative diagnoses (n = 10) had PAG concentration higher than the threshold (>/=1.8 ng/mL) for diagnosing pregnancy. The specificity of TRP for detecting non-pregnant buffalo cows ranged between 90.9%, and 100% between days 31 and 55. All cases of false positive diagnoses (n = 5) made by TRP had PAG concentrations lower than the threshold for diagnosing pregnancy. It could be concluded that TRP is an accurate method for diagnosing pregnant and non-pregnant buffalo cows from day 46 after breeding. [less ▲]

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See detailTools to assess iodine deficiency in calves born from deficient and non deficient dams
Guyot, Hugues ULg; Uyttenhoef, Aude; Lebreton, Pascal et al

Poster (2006)

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See detailPregnancy-associated glycoproteins in the ruminants: inactive members of the Aspartic Protease family
Beckers, Jean-François ULg; Drion, Pierre ULg; Garbayo, J. M. et al

in Proceedings of the Middel European Buiatrics Congress, 1998 (1998, May 21)

Proteins secreted by the placenta, when detected in the peripheral circulation of the mother, can be useful indicators of both pregnancy and feto-trophoblast well-being (1-3). In 1982, Butler et al. (4 ... [more ▼]

Proteins secreted by the placenta, when detected in the peripheral circulation of the mother, can be useful indicators of both pregnancy and feto-trophoblast well-being (1-3). In 1982, Butler et al. (4) isolated two pregnancy-specific proteins (PSPA and PSPB) from bovine placental membranes. PSPA was identified as a-fetoprotein which is not strictly limited to pregnancy, while PSPB was confirmed as placenta’s and pregnancy’s specific (5). PSPB was characterized as a glycoprotein showing relative molecular masses (Mr) between 47 and 53 kDa and presenting different isoelectric points (from 4.0 to 4.4). The Mr of PSPB was similar to the Mr of the molecule isolated by Laster in 1977 (6). In 1991, Zoli et al. (7) purified a pregnancy-associated glycoprotein (PAG), later designated PAG_1 and presently designated as PAG_I_ 67 considering its Mr. When isolated from bovine fetal cotyledons PAG_I_67 was an acidic glycoprotein of 67 kDA. Four isoforms (PI : 4.4, 4.6, 5.2 and 5.4) were detected in the initial preparation. Later molecular cloning studies showed that the PSPB and PAG_I_67 were closely related in primary structure (8). These glycoproteins (either PSPB and PAG_I_67) could be detected in the maternal circulation at around the time that the trophoblast formed definitive attachment to the uterine wall (figures A & B). Concentrations increased gradually therefore and reached peak values just before parturition at about 1 to 5 mg/ml (9).The PSPB and PAG molecules are routinely determined in peripheral maternal blood as pregnancy markers in cattle (5, 9,10). Glycoproteins immunologically related to PAG_I_67 and PSPB have been isolated and partially characterized from ovine fetal cotyledons: oPAG later designated oPAG_I (11) and oPSPB (12). They also have been detected in maternal blood by week third (12) or fourth (13) after breeding. Different forms (differing in Mr and isoelectric point) were characterized after isolation from sheep cotyledons cultured in vitro (14). Very recently, 3 different PAGs were characterized from goat placenta having Mr of 55, 59 and 62 kDa. Each of them presented various isoeletric points (15). In 1991, Xie et al., cloned PAG (now known as PAG_I_67) from late bovine and ovine placenta by screening cDNA libraries with two anti-PAG antisera (16). The bovine and ovine cDNAs encoding PAG_I shared 86% nucleotide sequence identic with one and other and encoded proteins of 380 and 382 aminoacids respectively including a 15 aminoacid signal sequence. However, protein sequence data, (peptidic sequencing) already showed the first aminoacid of the bovine PAG_I_67 was an arginine that corresponded to another one located at position 39, downstream of the side of signal sequence cleavage, indicating that PAG_I_67 undergoes post-translational modifications from a proform. [less ▲]

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See detailRepeated high doses administration of eCG in cows submitted to an ovum pick-up experimental protocol: plasmatic eCG binding rate follow up
Drion, Pierre ULg; De Roover, R.; Houtain JY et al

(1997, September 12)

This study performed plasma eCG binding rates assay on 8 cows submitted to repeated and high doses eCG administration in an ovum pick-up experimental protocol (de Roover et al., Abs.). The cows were ... [more ▼]

This study performed plasma eCG binding rates assay on 8 cows submitted to repeated and high doses eCG administration in an ovum pick-up experimental protocol (de Roover et al., Abs.). The cows were divided in two groups. For each cow, blood samples were taken three times a week, before and during the period of treatments. Cows of the first group received 10 injections of eCG (2000UI; 4x1000 UI; 5x2000 UI) while cows of the second group received 5 eCG treatments (2000 UI).Statistical analysis of results was made using the "SAS General Linear Model Procedure (GLM)" with binding rate considered as dependent variable and animals, treatment, and stade (before or after treatment) as independent variables. A significative (p<0.01) difference was found between binding rates before and after the treatment in either the first or the second group. The two treatments were found to induce a significative (p<0.001) increase in binding rates when compared with values obtained before treatments. Even if all cows responded to treatments by a binding rate increase, the effect of the treatment differed significantly (p<0.01) from one cow to another which could be explained by a large individual variability. From this experiment, we conclude that production of eCG antibodies is a wide response in the cows herein presented and repeatedly submitted to high doses of eCG. This immune response appeared after three to five injections and binding rates remained elevated over weeks following the latest injection. Special care should be given to this active immunisation -that could explain refractoriness to treatments- if such repeated administrations high doses of eCG were applied in usual protocols. [less ▲]

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See detailBovine pregnancy-associated glycoprotein profiles as indicators of trophoblastic function after in vitro manipulation or culture
Ectors, Fabien ULg; Sulon, José; Delval, A. et al

in Reproduction in Domestic Animals (1997, February), 32(1-2), 52

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See detailInterests of pregnancy follow-up in cows after embryo transfer : special focusing on IVP & NT
Ectors, Fabien ULg; Drion, Pierre ULg; Delval, A. et al

(1996, September 13)

In this report, pregnancies were obtained after extreme in vitro conditions- IVM/F/C of the donor embryos, - IVM, enucleation and artificial activation of the recipient oocytes, - nuclear transfer and ... [more ▼]

In this report, pregnancies were obtained after extreme in vitro conditions- IVM/F/C of the donor embryos, - IVM, enucleation and artificial activation of the recipient oocytes, - nuclear transfer and - IVC of the reconstituted embryos. Even if the incidence of this syndrome is relatively low after embryo transfer, a possible increasing of its occurence cannot be excluded in correlation with an incomplete maturation of oocytes at the time of fertilization, smaller follicles giving non competent or partially competent oocytes. An other explanation of this syndrome resulting in the higher variation in newborn calves weight may be also partly explained by the in vitro conditions. The gametes and/or embryos may be submitted to media containing embryotoxic substances. In the other hand, gametes and/or embryos may not found embryotrophic substances in the media like growth factors... Owing to this phenomenon, strict recommendations should be followed concerning rigorous follow-up of pregnancies obtained after transfer of IVM/IVF/IVC or cloned embryos by pregnancy proteins (PSPB, PAG...) or hormone (placental lactogen, estrone sulfate) assay and, after birth, macroscopic examinations of newborn, cord and caroncules. [less ▲]

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See detailDiagnostic performance of the thirty-four hour dexamethasone suppression test
Ansseau, Marc ULg; Doumont, Arlette; Cerfontaine, Jean-Luc et al

in Psychoneuroendocrinology (1985), 10

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