References of "Sulon, J"
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See detailÉtude de la cyclicité en relation avec le poids vif et l’état corporel chez les agnelles Ouled Djellal nées en automne dans la région Est de l’Algérie
Lamrani, F; Benyounes, A; Sulon, J et al

in Annales de Médecine Vétérinaire (2012), 156

The objective of the present work was to describe the cyclicity of Ouled Djellal ewes born one year before regarding their live weight and body condition score, during the autumn season. The study has ... [more ▼]

The objective of the present work was to describe the cyclicity of Ouled Djellal ewes born one year before regarding their live weight and body condition score, during the autumn season. The study has been driven in the region of Guelma, East of Algeria, on 104 ewes belonging to two farms. At the beginning of the study, ewes from farms A and B had similar ages but differed in live weight and body condition scores. The concentration of progesterone wasdetermined by radioimmunoassay. The level of spontaneous cyclicity, as well as the plasmatic level of the progesterone, were influenced significantly by both live weight and body condition score (P<0.0001). If the first was in favor of ewes with high body weight and body condition score, the second seemed to be higher in slim cyclic ewes. The body fatness appears to influence positively the age of puberty. [less ▲]

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See detailDetection of placental lactogens in Swamp buffalo by radioimmunoassay technique.
Hanh, N. V.; Huu, Q. X.; Uoc, N. T. et al

in Reproduction, Fertility and Development (2009), 21(1), 152-153

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See detailDetection of placental lactogens in swamp buffalo by radioimmunoassay technique
Hanh, N. V.; Huu, Q. X.; Uoc, N. T. et al

in Reproduction, Fertility and Development (2009), 21(1), 152-153

Ruminant placental lactogens (PL) are members of the growth factor/prolactin (GH/PRL) family. They are synthesized by trophectodermal binucleate cells. There is evidence to suggest that PL is involved in ... [more ▼]

Ruminant placental lactogens (PL) are members of the growth factor/prolactin (GH/PRL) family. They are synthesized by trophectodermal binucleate cells. There is evidence to suggest that PL is involved in control of fetal growth, through actions in both the maternal and fetal compartments, as well as in influencing mammary growth during pregnancy (Byatt JC et al. 1992 J. Anim. Sci. 70, 2911–2923). The structure and biology of PL have been studied in the cow, sheep, goat, human, and mice. The maternal concentration of PL is 100- to 1 000-fold greater in pregnant sheep and goats than in cows but no information exists about PL concentration in buffalo. The aim of the present study was to evaluate the ability to detect PL in buffalo fluids by using bovine PL antibody. Samples were collected in the slaughterhouse immediately after animal slaughter. The fetuses were measured after heart blood collection. A bPL RIA system was used to determine the bPL concentrations in the buffalo samples (Alvarez-Oxiley AV et al. 2007 Reprod. Fertil. Dev. 19, 877–885). The rbPL molecules were radio-iodinated with [125]I-Na by using the lactoperoxidase method (Thorell JI and Johansson BG 1971 Biochim. Biophys. Acta 251, 363–369). Concentrations of buffalo PL are presented in Table 1. In this RIA system, the minimum detected value was 0.068 ng mL–1, and the binding competition curves of bovine PL standard and buffalo fluids dilution using bovine PL antibody were paralleled in all kinds of samples. The lowest concentration was detected in allantoid fluid and the greatest concentration in fetal plasma (P < 0.05). Study of the biology of PL in buffalo has proved difficult because the concentration of PL in all buffalo fluids is very low. Furthermore, the research concerning buffalo PL function required in vivo experiments. Existing data suggest that at least the concentration of buffalo PL is different from cattle and other smaller domestic ruminants. In conclusion, our results provide preliminary information about concentrations of PL in buffalo fluids. [less ▲]

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See detailRéussite à l'insémination en élevages ovins laitiers pyrénéens : facteurs de variantion liés aux conduites de troupeaux
Arranz, J. M.; Freret, S.; Fidelle, F. et al

in Proceedings de la 15e Journée sur les Rencontres de Recherche chez les Ruminants (2008)

En élevage ovin-lait, la réussite à l’insémination artificielle (IA) est un facteur clé pour la maîtrise de la reproduction et la mise en œuvre des schémas de sélection. Elle a été évaluée : 1) par dosage ... [more ▼]

En élevage ovin-lait, la réussite à l’insémination artificielle (IA) est un facteur clé pour la maîtrise de la reproduction et la mise en œuvre des schémas de sélection. Elle a été évaluée : 1) par dosage de la PAG plasmatique vingt huit jours après l’insémination ; 2) par l’analyse des résultats de mise bas (MB). La différence entre ces deux valeurs permet d’estimer le niveau de pertes embryonnaires et avortements. L’étude porte sur quatre-vingt cinq lots d’insémination (8841 brebis de races Manech / Latxa tête rousse et tête noire principalement) réalisés de mai à août 2007, dans trois centres d’insémination (CIA) transfrontaliers : Navarre, communauté autonome d’Euskadi et Pyrénées Atlantiques. Les données de contrôle laitier et des CIA ont été mobilisées pour étudier les facteurs de variation de la fertilité individuelle. Un deuxième volet de l’étude, à l’échelle des lots, traite des conduites d’élevage pendant la période de lutte. Il s’appuie sur des enquêtes (alimentation, chantiers d’IA, sanitaire). La fertilité à vingt huit jours s’élève à 61,3 % et celle à la MB à 55,2 %. Les pertes sont en moyenne de 6,2 %. Pour 2/3 des lots elles sont inférieures à 7 %. L’analyse des facteurs individuels, tant pour la fertilité à vingt huit jours qu’à la MB, confirme les effets classiques des facteurs de variation de la fertilité à l’IA : intervalle IA-MB précédente, âge des femelles, mode de reproduction de la campagne précédente, production laitière au moment de l’IA, qualité de la semence. Les niveaux de pertes sont plus élevés pour les jeunes ou lorsque l’intervalle est court. Au niveau des conduites d’élevage, on observe une interaction forte race*région associée à des périodes d’IA et des conduites alimentaires différentes, mais avec des effets peu ou pas significatifs de ces conduites. Les enquêtes sanitaires font apparaître des niveaux de risques et des pathologies comparables d’une région à l’autre et élevés. Pour les lots à pertes élevées, celles-ci ont pu être reliées à des problèmes pathologiques particuliers, mais non prévisibles. Une telle approche, assez complète et globale des conduites, aura permis de renouveler le conseil en élevage et d’élargir les capacités d’intervention des techniciens. [less ▲]

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See detailPlasma luteinizing hormone and progesterone concentrations in Azawak zebu cows submitted to different estrus synchronization protocols.
Zongo, M.; Pitala, W.; Sawadogo, L. et al

in Revue d'Elévage et de Médecine Vétérinaire des Pays Tropicaux (2008), 61(3-4), 221-227

The percentage of estrus induction and plasma LH concentrations were examined in 15 Azawak zebu cows submitted to two different estrus synchronization protocols. In the first treatment (T1, n = 9), the ... [more ▼]

The percentage of estrus induction and plasma LH concentrations were examined in 15 Azawak zebu cows submitted to two different estrus synchronization protocols. In the first treatment (T1, n = 9), the cows received a norgestomet ear-implant for 10 days associated with estradiol valerate, prostaglandin F2alpha (PGF2alpha) and pregnant mare serum gonadotropin (PMSG) injections. Treatment 2 (T2, n = 6) consisted in two PGF2alpha injections 11 days apart, followed by the administration of PMSG two days after the second prostaglandin injection. Blood samples were collected every three hours during five days after implant removal (T1) or PMSG injection (T2) for LH measurements. The proportion of induced estrus was higher in T2, but the difference was not significant. In general, the elapsed time until the estrus onset tended to be shorter in norgestomet-treated animals (35.9 ± 3.9 h) than in prostaglandin-treated ones (49.5 ± 5.8 h). Seven out of nine norgestomet-treated cows, and only two out of the six treated with PGF2alpha, presented a peak of LH. The mean interval from the end of treatments to LH peak tended to be longer in PGF2-treated females than in norgestomet-treated ones. One female presenting abnormally high LH concentrations after implant removal did not show a peak of LH during the observation period. [less ▲]

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See detailAssessment of stress level in horses (Equus caballus): behavioural and physiological measurements in hospital.
Peeters, Marie ULg; Péters, F.; Sulon, J. et al

Conference (2008)

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See detailInfluence of continuous breeding goats on return to cyclicity in seasonal breeding goats
Prvanovic, N; Vince, S; Duricic, D et al

in Reproduction in Domestic Animals (2007), 42

The boer goats which originated from the South African Republic are continous breeding animals when kept in the northern hemisphere and they kidd three times in 2 years. In this study, a mixed herd was ... [more ▼]

The boer goats which originated from the South African Republic are continous breeding animals when kept in the northern hemisphere and they kidd three times in 2 years. In this study, a mixed herd was monitored to determine whether boer goats could induce return to cyclicity in sesonal breeding goats. All goats (n = 20) were pluriparous and kidded during January and February. During January a mixed group was formed (10 boer goats kept together with 10 domestic breed goats). At the same time a group of domestic breed goats (n = 12), kept in the same manner, 40 km away from the experimental group was used as controls. Blood samples were collected every 72 h to determine level of progesterone in both herds. Goats were monitored at the same time to observe for signs of oestrus. Progesterone concentrations in sera samples were determined using a RIA method. Data were analysed by ANOVA using Statistica software. We observed visual oestrus in all boer goats and in eight domestic breed goats from the mixed group during March and April. Progesterone concentrations confirmed the presence of functional corpora lutea between two visual cycles in these animals. No visual oestrus or increases in progesterone were observed in the control group of goats. In conclusion, continuous breeding boer goats induced oestrus in 80% (n = 8) of seasonal breeding goats when kept in a mixed herd. [less ▲]

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See detailThe Pregnancy-Associated Glycoproteins: Biochemical Aspects and Clinical Application for Pregnancy Follow-up
Melo de Sousa, Noelita ULg; Barbato, O.; Bella, Amina ULg et al

Conference (2007)

Pregnancy diagnosis is an important part in reproduction management of ruminants. In the last years, a large polymorphic family of placenta-expressed proteins has been discovered in ruminant species and ... [more ▼]

Pregnancy diagnosis is an important part in reproduction management of ruminants. In the last years, a large polymorphic family of placenta-expressed proteins has been discovered in ruminant species and used for pregnancy diagnosis. Members of this family are named pregnancy-associated glycoproteins (PAG), being synthesized in the mono- and binucleate cells of the ruminant’s trophectoderm. Part of them are released in the maternal blood circulation where they can be assayed by different laboratory techniques. Due to large variety of expressed molecules and to large variations in the post-translational processing of the PAG, different immuno-systems present different ability to quantify the PAG released in blood. The sensitivity (92 to 100%) and specificity of PAG radioimmunoassay when used for pregnancy diagnosis are very high. The assay of PAG can also bring very interesting information for researchers working in programs focused on the study of embryonic and fetal mortalities, as well as on embryo biotechnology (ET, FIV, clonage), animal nutrition, or infections diseases resulting in pathologies affecting the pregnancy. [less ▲]

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See detailComparison of ultrasound and pregnancy associated glycoproteins to diagnose and follow ongoing or failing pregnancy in pregnant heifers on the market
Prvanović, N; Beckers, Jean-François ULg; Sulon, J et al

in Slovenian veterinary research (2006), 43(10), 41-43

The objective of the present study was to compare transrectal ultrasonography and pregnancy associated glycoprotein (PAG) tests for determination of pregnancy in 72 Holstein-Friesian heifers imported from ... [more ▼]

The objective of the present study was to compare transrectal ultrasonography and pregnancy associated glycoprotein (PAG) tests for determination of pregnancy in 72 Holstein-Friesian heifers imported from Holland to Croatia. All heifers came to Croatia with written confirmation of pregnancy. During caranteen we preformed rectal and ultrasound exploration of heifers and simultaneously collected blood samples from each heifer. PAG concentrations were determined by a RIA immunoassay using bovine PAG 67 KDa as a tracer and standard. In our study 68 heifers were confirmed to be pregnant by both methods and 4 cows were confirmed for abortion by PAG test and nonpregnant according to ultrasound checking. Transrectal ultrasonography is accurate test for pregnancy diagnosis in pregnant heifers but it is impossible to say only on basis of ultrasound examination if heifers conceived and aborted or they failed to conceive in the first place. PAG RIA test is not only highly accurate and reliable method to distinguish between pregnant and nonpregnant heifers on the market. It is also useful to determine whether heifers were pregnant and embryo died and was absorbed during the transfer or heifers failed to conceive. It is possible because level of PAG persists in circulation after abortion or embrional death. In doubtful cases it can be used as a proof of pregnancy existence in heifers later confirmed to be nonpregnant. [less ▲]

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See detailFetal profiles of bovine placental lactogen (PL) and insulin like growth factors (IGF-I and IGF–II) through the late gestation
Alvarez Oxiley, Andrea Vivian ULg; Melo de Sousa, Noelita ULg; Sulon, J. et al

in Prooceedings of the 10e Journée de Rencontre Bioforum (2006)

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See detailThe ability of five radioimmunoassay systems to detect early pregnancy-associated glycoprotein in bovine plasma.
Ayad, A.; Melo de Sousa, Noelita ULg; Sulon, J. et al

in Proceedings of the 22nd Scientific Meeting of the European Embryo Transfer Association (2006)

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See detailProgesterone, luteinizing hormone, prolactin and pregnancy-associated glycoproteins during the first trimester pregnancy in cattle.
Ayad, A.; Melo de Sousa, Noelita ULg; Sulon, J. et al

in Proceedings of the 22nd Scientific Meeting of the European Embryo Transfer Association (2006)

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See detailSpecificity of different RIA systems for measurement of bovine pregnancy associated glycoproteins against carbohydrates and placental hormones.
Ayad, A.; Melo de Sousa, Noelita ULg; Sulon, J. et al

in Proceedings of the 10e Journée de Rencontre Bioforum (2006)

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See detailSuivi de la gestation chez la brebis Ouled Djellal par dosage de la protéine associée a la gestation et de la progestérone.
Benyounes, A.; Lamrani, F.; Melo de Sousa, Noelita ULg et al

in Revue d'Elévage et de Médecine Vétérinaire des Pays Tropicaux (2006), 59(1-4), 65-73

L’objectif de la présente étude a été la caractérisation des profils plasmatiques de la protéine associée à la gestation (PAG) et de la progestérone au cours de la gestation et deux semaines après le part ... [more ▼]

L’objectif de la présente étude a été la caractérisation des profils plasmatiques de la protéine associée à la gestation (PAG) et de la progestérone au cours de la gestation et deux semaines après le part chez la race Ouled Djellal. Vingt-cinq brebis ont été prélevées hebdomadairement après synchronisation des chaleurs et monte naturelle. Les concentrations de PAG et de progestérone ont été déterminées par dosage radio-immunologique. Deux femelles n’ont pas été fécondées tandis que 23 ont eu des gestations considérées comme normales (n=18) ou pathologiques (n=5). Chez 18 brebis (12 gestations simples et 6 multiples), les concentrations de PAG et de progestérone ont été significativement influencées par le stade de la gestation (P<0.0001) et non par le nombre de fœtus. Des profils individuels ont été décrits chez 7 brebis dont 3 femelles ayant eu des pertes embryonnaires, et 2 brebis ayant avorté (66e et 110e jours), et enfin chez 2 brebis ayant donné naissance à terme chacune à un agneau vivant et expulsé au moment de la mise-bas 1 ou 2 fœtus momifié(s). Les concentrations en PAG ont été plus faibles chez les femelles ayant de fœtus momifiés en parallèle avec des fœtus vivants. [less ▲]

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See detailPlasma urea nitrogen and phosphorus in relation to pregnancy rate in dairy sheep
Karen, A.; Kovacs, P.; Beckers, Jean-François ULg et al

in Proceedings of the 24th World Buiatrics Congress (2006)

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See detailMeasurement of progesterone concentration in plasma samples: comparison of three radioimmunoassay procedures
Legras, B.; Beckers, Jean-François ULg; Sulon, J.

in Reproduction (Cambridge, England). Abstract Series (2004), 31

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See detailEffect of number of lambs, their sex and birth weignt on ovine pregnancy associated glycoprotein (ovPAG) concentrations
Vandaele, L; Verberckmoes, S; De Cat, S et al

in Journal of Reproduction & Fertility. Abstract Series (2004), 16(2), 192193

Early pregnancy detection and prediction of the number of lambs would be profitable for sheep breeders, as this enables nourishment of pregnant ewes to their individual needs. Nonpregnant ewes can be ... [more ▼]

Early pregnancy detection and prediction of the number of lambs would be profitable for sheep breeders, as this enables nourishment of pregnant ewes to their individual needs. Nonpregnant ewes can be separated and sold before winter. Prediction of sex is interesting for the curiosity of impatient breeders. Early pregnancy detection (before 45 days after mating) by means of ovine pregnancy-associated glycoproteins (ovPAGs) has proved to be a more reliable test than the commonly used transrectal ultrasound. The concentration of ovPAG has been claimed to be related to the number of lambs, their sex and birth weight, but contradictory results were obtained in different small-scale studies. In this study the correlation between number of lambs, their sex and birth weight and the ovPAG concentration in the serum of the ewes was examined. In a field trial, 160 and 70 ewes of the Texel and Suffolk breed, respectively, housed on 6 different farms, were synchronized by means of intravaginal sponges impregnated with 40 mg cronolone (Chronogest®, Intervet, Boxmeer, The Netherlands). At the moment of synchronisation (T0) and at 25 (T25), 35 (T35) and 45 (T45) days after mating or artificial insemination, blood samples were taken by jugular vein puncture. A heterologous radioimmunoassay (RIA) was used to determine the ovPAG concentration. In the heterologous RIA boPAG molecules were used as tracer, and ovPAG molecules as standard, while rabbit antibodies against ovPAG were used as antiserum. At parturition, number, sex and birth weight of the lambs were registered and their effect on ovPAG concentrations was statistically evaluated. No significant difference in ovPAG concentration was found between ewes pregnant of a single male or female lamb (P = 0.29). ovPAG concentrations were affected by the total birth weight (P < 0.05). In ewes pregnant with multiples, the ovPAG concentrations were higher (P < 0.01) than in ewes pregnant with singles at T25, T35 and T45, respectively. Because ovPAG concentrations were affected by the farm, it was impossible to predict the number of lambs by the ovPAG level. In conclusion, ovPAG concentrations were not affected by the sex of the lamb. However, ovPAG concentrations were related to the total birth weight. Within the farm, increased ovPAG concentrations may indicate pregnancy of multiples. [less ▲]

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See detailOvine pregnancy-associated glycoprotein profiles in ewes pregnant with singletons or twins
Vandaele, L.; Verberckmoes, S.; Van Soom, A. et al

in Reproduction in Domestic Animals (2004), 39(4), 278

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See detailUse of heterologous and homologous radioimmunoassay (RIA) for ovine pregnancy-associated glycoprotein (ovPAG) determination to detect pregnancy in sheep
Vandaele, L.; Verberckmoes, S.; De Cat, S. et al

in Reproduction (Cambridge, England). Abstract Series (2004), 31

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