References of "Stefanuto, Pierre-Hugues"
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See detailFast Chromatographic Method for Explosive Profiling
Stefanuto, Pierre-Hugues ULg; Perrault, Katelynn A.; Focant, Jean-François ULg et al

in Chromatography (2015), 2

Security control is becoming a major global issue in strategic locations, such as airports, official buildings, and transit stations. The agencies responsible for public security need powerful and ... [more ▼]

Security control is becoming a major global issue in strategic locations, such as airports, official buildings, and transit stations. The agencies responsible for public security need powerful and sensitive tools to detect warfare agents and explosives. Volatile signature detection is one of the fastest and easiest ways to achieve this task. However, explosive chemicals have low volatility making their detection challenging. In this research, we developed and evaluated fast chromatographic methods to improve the characterization of volatile signatures from explosives samples. The headspace of explosives was sampled with solid phase micro-extraction fiber (SPME). Following this step, classical gas chromatography (GC) and comprehensive two-dimensional GC (GC×GC) were used for analysis. A fast GC approach allows the elution temperature of each analyte to be decreased, resulting in decreased thermal degradation of sensitive compounds (e.g., nitro explosives). Using fast GC×GC, the limit of detection is further decreased based on the cryo-focusing effect of the modulator. Sampling of explosives and chromatographic separation were optimized, and the methods then applied to commercial explosives samples. Implementation of fast GC methods will be valuable in the future for defense and security forensics applications. [less ▲]

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See detailGCxGC-TOFMS and supervised multivariate approaches to study human cadaveric decomposition olfactive signatures
Stefanuto, Pierre-Hugues ULg; Perrault, Katelynn A.; Stadler, Sonja et al

in Analytical Bioanalytical Chemistry (2015), 407(16), 4767-4778

In forensic thanato-chemistry, the understanding of the process of soft tissue decomposition is still limited. A better understanding of the decomposition process and the characterization of the ... [more ▼]

In forensic thanato-chemistry, the understanding of the process of soft tissue decomposition is still limited. A better understanding of the decomposition process and the characterization of the associated volatile organic compounds (VOC) can help to improve the training of victim recovery (VR) canines, which are used to search for trapped victims in natural disasters or to locate corpses during criminal investigations. The complexity of matrices and the dynamic nature of this process require the use of comprehensive analytical methods for investigation. Moreover, the variability of the environment and between individuals creates additional difficulties in terms of normalization. The resolution of the complex mixture of VOCs emitted by a decaying corpse can be improved using comprehensive two-dimensional gas chromatography (GC× GC), compared to classical singledimensional gas chromatography (1DGC). This study combines the analytical advantages of GC×GC coupled to timeof- flight mass spectrometry (TOFMS) with the data handling robustness of supervised multivariate statistics to investigate the VOC profile of human remains during early stages of decomposition. Various supervised multivariate approaches are compared to interpret the large data set. Moreover, early decomposition stages of pig carcasses (typically used as human surrogates in field studies) are also monitored to obtain a direct comparison of the two VOC profiles and estimate the robustness of this human decomposition analog model. In this research, we demonstrate that pig and human decomposition processes can be described by the same trends for the major compounds produced during the early stages of soft tissue decomposition. [less ▲]

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See detailExploring new dimensions in cadaveric decomposition odour analysis
Stefanuto, Pierre-Hugues ULg; Perrault, Katelynn; Lloyd, Rebecca et al

in Analytical Methods (2015), 7

This study demonstrates the first documented use of comprehensive two-dimensional gas chromatography – high-resolution time-of-flight mass spectrometry (GC×GC-HRTOFMS) for volatile organic compound ... [more ▼]

This study demonstrates the first documented use of comprehensive two-dimensional gas chromatography – high-resolution time-of-flight mass spectrometry (GC×GC-HRTOFMS) for volatile organic compound analysis in the forensic sciences. High-resolution mass spectral data provided higher confidence in analyte identification. GC×GC-HRTOFMS will be valuable for future studies of decomposition odour and other complex volatile matrices. [less ▲]

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See detailMeasurement of Human Internal Cadaveric VOC's by GCxGC-HRTOFMS
Stefanuto, Pierre-Hugues ULg; Varlet, Vincent; Silke et al

Conference (2015, January)

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See detailComparison of the Decomposition VOC Profile during Winter and Summer in a Moist, Mid-latitude (Cfb) Climate
Forbes, Shari L.; Perrault, Katelynn A.; Stefanuto, Pierre-Hugues ULg et al

in PLoS ONE (2014), 9(e113681), 1-11

The investigation of volatile organic compounds (VOCs) associated with decomposition is an emerging field in forensic taphonomy due to their importance in locating human remains using biological detectors ... [more ▼]

The investigation of volatile organic compounds (VOCs) associated with decomposition is an emerging field in forensic taphonomy due to their importance in locating human remains using biological detectors such as insects and canines. A consistent decomposition VOC profile has not yet been elucidated due to the intrinsic impact of the environment on the decomposition process in different climatic zones. The study of decomposition VOCs has typically occurred during the warmer months to enable chemical profiling of all decomposition stages. The present study investigated the decomposition VOC profile in air during both warmer and cooler months in a moist, mid-latitude (Cfb) climate as decomposition occurs year-round in this environment. Pig carcasses (Sus scrofa domesticus L.) were placed on a soil surface to decompose naturally and their VOC profile was monitored during the winter and summer months. Corresponding control sites were also monitored to determine the natural VOC profile of the surrounding soil and vegetation. VOC samples were collected onto sorbent tubes and analyzed using comprehensive two-dimensional gas chromatography – time-of-flight mass spectrometry (GC6GC-TOFMS). The summer months were characterized by higher temperatures and solar radiation, greater rainfall accumulation, and comparable humidity when compared to the winter months. The rate of decomposition was faster and the number and abundance of VOCs was proportionally higher in summer. However, a similar trend was observed in winter and summer demonstrating a rapid increase in VOC abundance during active decay with a second increase in abundance occurring later in the decomposition process. Sulfur-containing compounds, alcohols and ketones represented the most abundant classes of compounds in both seasons, although almost all 10 compound classes identified contributed to discriminating the stages of decomposition throughout both seasons. The advantages of GC6GC-TOFMS were demonstrated for detecting and identifying trace levels of VOCs, particularly ethers, which are rarely reported as decomposition VOCs. [less ▲]

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See detailDeveloping the sixth sense of thanato-chemistry
Stefanuto, Pierre-Hugues ULg; Perrault, Katelynn; Varlet, Vincent et al

Scientific conference (2014, November)

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See detailTaking Cadaveric Decomposition Chemistry out of Flatland
Stefanuto, Pierre-Hugues ULg; Perrault, K; Stadler, S et al

Conference (2014, July 07)

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See detailThe Sweet Smell of Science
Stefanuto, Pierre-Hugues ULg; Focant, Jean-François ULg

Scientific conference (2014, July)

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See detailReading Cadaveric Decomposition Chemistry with a New Pair of Glasses
Stefanuto, Pierre-Hugues ULg; Perrault, Katelynn; Stadler, Sonja et al

in CHEMPLUSCHEM (2014), 79(6), 786-789

Smell it out! Thermal desorption has been coupled with comprehensive two-dimensional gas chromatography time of-flight mass spectrometry for the characterization of a cadaveric volatile organic compound ... [more ▼]

Smell it out! Thermal desorption has been coupled with comprehensive two-dimensional gas chromatography time of-flight mass spectrometry for the characterization of a cadaveric volatile organic compound mixture producedduring the active decay stage (see figure). The combined use of the Fisher ratio and principal component analysis provided further characterization of the chemical profile of decomposition. [less ▲]

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See detailPersistence of decomposition odour in soil analysed by GC×GC-TOFMS
Perreault, K; Stefanuto, Pierre-Hugues ULg; Stuart, B et al

Conference (2014, May 22)

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See detailTrappist Beers: Exploring the Cloak of Secrecy by GC×GC-TOFMS
Allen, C; Loughnane, C; Stefanuto, Pierre-Hugues ULg et al

Poster (2014, May 21)

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See detailPostmortem Measurement of Cadaveric VOCs in Human Internal Cavity Gases
Stefanuto, Pierre-Hugues ULg; Varlet, V; Grabherr, S et al

Conference (2014, May 20)

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See detailAvancées récentes dans l'investigation olfactive de la mort
Stefanuto, Pierre-Hugues ULg; Focant, Jean-François ULg

Scientific conference (2014, May 07)

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See detailImproving our vision on human cadaveric decomposition chemistry
Stefanuto, Pierre-Hugues ULg; Stadler, S; Perrault, K et al

Conference (2014, April 15)

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See detailFast chromatographic method for explosives profiling
Stefanuto, Pierre-Hugues ULg; Perrault, K; Perrault, K et al

in Hyphenated Techniques in Chromatography HTC-13 - Book of abstracts (2014, January)

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See detailGC×GC-TOFMS identification of new cadaveric VOCs
Pesesse, Romain ULg; Stefanuto, Pierre-Hugues ULg; Stadler, S et al

in Hyphenated Techniques in Chromatography HTC-13 - Book of abstracts (2014, January)

Detailed reference viewed: 37 (4 ULg)