References of "Steele, M"
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See detailCharacterization of a set of variable number of tandem repeat markers conserved in Bovidae
Georges, Michel ULg; Gunawardana, A.; Threadgill, D. et al

in Genomics (1991), 11(1), 24-32

Screening purpose-built libraries with minisatellite probes, we have isolated 36 bovine variable number of tandem repeat markers (VNTRs) characterized by a mean heterozygosity of 59.3 within the American ... [more ▼]

Screening purpose-built libraries with minisatellite probes, we have isolated 36 bovine variable number of tandem repeat markers (VNTRs) characterized by a mean heterozygosity of 59.3 within the American Holstein breed. Matching probabilities and exclusion powers were estimated by Monte-Carlo simulation, showing that the top 5 to 10 markers could be used as a very efficient DNA-based system for individual identification and paternity diagnosis. The isolated VNTR systems should contribute significantly to the establishment of a bovine primary DNA marker map. Linkage analysis, use of somatic cell hybrids, and in situ hybridization demonstrate that these bovine VNTRs are scattered throughout the bovine genome, without evidence for proterminal confinement as in the human, and that at least some of them are organized as clusters. Moreover, Southern blot analysis and in situ hybridization demonstrate conservation of sequence and map location of minisatellites within Bovidae [less ▲]

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Peer Reviewed
See detailGeneration of bovine multisite haplotypes using random cosmids
Steele, M.; Georges, Michel ULg

in Genomics (1991), 10

One hundred ten random cosmids were used to probe Southern blots of DNA from nine unrelated cattle digested with 12 restriction enzymes. Although only one-third of the expected fragments were explored, 85 ... [more ▼]

One hundred ten random cosmids were used to probe Southern blots of DNA from nine unrelated cattle digested with 12 restriction enzymes. Although only one-third of the expected fragments were explored, 85% of the cosmids revealed at least one polymorphism. The mean heterozygosity of the generated haplotypes was estimated at 51.9%. A surprisingly high proportion of polymorphisms (approximately 25%) was attributed to insertion-deletion events, compensating for the lower level of nucleotide diversity observed in cattle (pi approximately 0.0007) compared to that in human. The mutation rate at cytosines in the CpG dinucleotide was estimated approximately 10 times higher than that at other nucleotides. When used in linkage studies, the generated markers should cover approximately 50% of the bovine genome. [less ▲]

Detailed reference viewed: 11 (1 ULg)