References of "Soto, R. P"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailKinetic and spectroscopic characterization of native and metal-substituted beta-lactamase from Aeromonas hydrophila AE036.
Hernandez Valladares, M.; Kiefer, M.; Heinz, U. et al

in FEBS letters (2000), 467(2-3), 221-5

Two metal ion binding sites are conserved in metallo-beta-lactamase from Aeromonas hydrophila. The ligands of a first zinc ion bound with picomolar dissociation constant were identified by EXAFS ... [more ▼]

Two metal ion binding sites are conserved in metallo-beta-lactamase from Aeromonas hydrophila. The ligands of a first zinc ion bound with picomolar dissociation constant were identified by EXAFS spectroscopy as one Cys, two His and one additional N/O donor. Sulfur-to-metal charge transfer bands are observed for all mono- and di-metal species substituted with Cu(II) or Co(II) due to ligation of the single conserved cysteine residue. Binding of a second metal ion results in non-competitive inhibition which might be explained by an alternative kinetic mechanism. A possible partition of metal ions between the two binding sites is discussed. [less ▲]

Detailed reference viewed: 37 (0 ULg)
Full Text
Peer Reviewed
See detailInteraction between Class B Beta-Lactamases and Suicide Substrates of Active-Site Serine Beta-Lactamases
Prosperi-Meys, C.; Llabres, Gabriel ULg; De Seny, Dominique ULg et al

in FEBS Letters (1999), 443(2), 109-11

The most widely used inactivators of active-site serine beta-lactamases behave as substrates of four class B metallo-beta-lactamases, but the efficiency of the catalytic process can vary by several orders ... [more ▼]

The most widely used inactivators of active-site serine beta-lactamases behave as substrates of four class B metallo-beta-lactamases, but the efficiency of the catalytic process can vary by several orders of magnitude. A comparison of the kinetic parameters for the alpha and beta isomers of 6-iodopenicillanic acid shows that there is no general preference for the alpha isomer and that the efficient hydrolysis of imipenem by these enzymes must rest on other factors. [less ▲]

Detailed reference viewed: 13 (0 ULg)