References of "Sneyers, M"
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See detailRelationships of growth hormone gene and milk protein polymorphisms to milk production traits in Simmental cattle
Falaki, M.; Prandi, A.; Corradini, C. et al

in Journal of Dairy Research (1997), 64(1), 47-56

The importance of milk proteins and the positive effect of administration of growth hormone (GH) on milk production, and the presence in some dairy cattle lines of greater GH concentrations prompted us to ... [more ▼]

The importance of milk proteins and the positive effect of administration of growth hormone (GH) on milk production, and the presence in some dairy cattle lines of greater GH concentrations prompted us to examine the presence of restriction fragment length polymorphism at the GH gene using the restriction enzyme TaqI and to investigate associations between this polymorphism in Simmental cows and bulls, as well as milk protein variants in Simmental cows, and milk production traits. Blood and milk were sampled from 279 Italian Simmental cows and semen was collected from 148 bulls of the same breed. Two fragment bands, denoted A and B, of 6200 and 5200 bp respectively, were examined and three patterns, AA, AB and BB, were found in both animal samples. All variants previously reported in other studies, for kappa, beta, and alpha(s1)-caseins, and beta-lactoglobulin, were found in the cows' samples. For the cows' samples, a BLUP (Best Linear Unbiased Predictor) analysis of results was performed using a REML (Restricted Maximum Likelihood) program and known heritabilities, whereas for bulls we have performed a General Linear Model analysis. The effect of GH gene polymorphism, using TaqI restriction enzyme, on milk production traits was not significant, but bulls of BB pattern had a higher breeding value for milk yield than AA bulls (P < 0.05). For the kappa-casein genotypic effects, cows of AB genotype gave milli with 1.53+/-0.70 g/kg less fat than cows of AA genotype. In addition, breeding values for milk protein content were significantly higher in BB bulls, with 0.87+/-0.32 and 0.71+/-0.34 g/kg more milk protein than AA and AB bulls respectively. Thus, our results revealed a GH gene polymorphism and indicated significant effects of milk protein polymorphisms on milk production traits in the Italian Simmental breed. [less ▲]

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See detailDissociation of increases in plasma insulin-like growth factor I and testosterone during the onset of puberty in bulls
Renaville, Robert ULg; Massart, Serge; Sneyers, M. et al

in Journal of Reproduction and Fertility (1996), 106(1), 79-86

The present study was conducted to examine the relationship between plasma concentrations of testosterone, insulin-like growth factor I (IGF-I) and IGF-binding proteins (IGFBPs) during puberty, in male ... [more ▼]

The present study was conducted to examine the relationship between plasma concentrations of testosterone, insulin-like growth factor I (IGF-I) and IGF-binding proteins (IGFBPs) during puberty, in male calves treated with GnRH or testosterone propionate. Twelve male Holstein calves (10 weeks old) were assigned to the control group (n = 6), the GnRH-treated group (n = 3) or the testosterone-treated group (n = 3). For 8 weeks, the GnRH-treated group received a single i.v. injection of GnRH (0.5 mu g kg(-1) body mass) each day while the testosterone-treated group received an i.m. injection of testosterone propionate (0.5 mg kg(-1) body mass) twice a day. The calves were studied until they were 200 days old. Hormone treatments were stopped one month after puberty was reached in the control group. Blood samples were collected every 30 min for 8 h every third day. Hormone concentrations were determined by radioimmunoassay. Western ligand blotting and immunoblotting, using monoclonal antibodies against IGFBP-2, and IGFBP-3, were used to characterize the IGF-binding proteins. In the control group, puberty occurred at about 120 days of age and was associated with an increase in concentrations of testosterone, IGF-I and IGFBP-3 and a decrease in concentration of IGFBP-2. In the GnRH-treated group, plasma testosterone remained low until 8 weeks after establishment of puberty in the control group (4 weeks after the end of treatment). In the testosterone-treated group, testosterone was high during the treatment period and then decreased to prepubertal values when treatment was stopped. Testosterone values increased again to reach postpubertal values 5 weeks after the end of hormone treatment. Nevertheless, independent of testosterone status, the profile of IGF-I and the IGFBPs in the GnRH- and testosterone-treated groups were parallel to that reported for the control group with the transition from prepubertal to adult values at about 120 days of age. In conclusion, concentrations of testosterone, IGF-I and IGFBP-3 increase together, but probably independently, during the onset of puberty in male calves. [less ▲]

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See detailRelationships of polymorphisms for growth hormone and growth hormone receptor genes with milk production traits for Italian Holstein-Friesian bulls
Falaki, M.; Gengler, Nicolas ULg; Sneyers, M. et al

in Journal of Dairy Science (1996), 79(8), 1446-1453

Allelic variation in the structural or regulatory sequences of growth hormone and its receptor genes might directly or indirectly affect milk traits. This possibility prompted us to investigate the ... [more ▼]

Allelic variation in the structural or regulatory sequences of growth hormone and its receptor genes might directly or indirectly affect milk traits. This possibility prompted us to investigate the eventual relationships of restriction fragment length polymorphisms at the locus of bovine growth hormone (using TagI and MspI restriction enzymes) and its receptor (using TaqI restriction enzyme) production traits of bulls. Holstein-Friesian bulls were used in this experiment, and data were analyzed with a fixed linear model. The restriction fragment length polymorphisms at the growth hormone locus did not affect the milk traits studied. Six restriction enzyme TaqI bands of 7.1, 6.2, 5.7, 5.4, 4.2, and 3.3 kb with nine patterns were observed after hybridization by a cDNA probe containing the coding sequences for the intracellular C-terminal part of the receptor. The effect of this polymorphism on PTA for milk protein percentage was highly significant and was favorable for the rare (6.6%) 5.7- and 5.4-kb pattern. Our results indicate that further study is needed to explain the DNA polymorphism and to obtain more definite conclusions about effects on milk traits. [less ▲]

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See detailTaqi Growth Hormone Gene Polymorphism And Milk Production Traits In Holstein-Friesian Cattle
Falaki, M.; Sneyers, M.; Prandi, A. et al

in Animal Science (1996), 63

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See detailCharacterization of growth hormone-binding protein in cattle plasma: prolactin-binding activity and 24-hour profile.
Massart, S.; Ban, A. M.; Renaville, Robert ULg et al

in Domestic Animal Endocrinology (1996), 13(1), 47-57

The purpose of this study was to characterize circulating growth hormone-binding proteins (GHBP) and prolactin-binding proteins (PRLBP) in cattle blood plasma. In particular, the 24-hr profile of these ... [more ▼]

The purpose of this study was to characterize circulating growth hormone-binding proteins (GHBP) and prolactin-binding proteins (PRLBP) in cattle blood plasma. In particular, the 24-hr profile of these molecules was investigated. The preincubation of bull plasma with iodinated bovine growth hormone (bGH) or bovine prolactin (bPRL), followed by gel filtration chromatography (Superdex 200; 1.6 x 60 cm column), resulted in the formation of essentially two complexes. The majority of [125I]bPRL eluted with the first one (M(r) approximately 600 kDa), whereas [125I]bGH mainly appeared in the second one (M(r) approximately 70 kDa). The fractions corresponding to these two peaks were analyzed by western ligand blotting (WLB), under reducing conditions. WLB revealed, respectively, 190-, 56-, 52-, and 28-kDa bands for the first peak and only 52- and 28-kDa bands for the second one. The nature of the 600-kDa peak is at present undetermined, but the 70-kDa one was previously identified as high-affinity GHBP. Displacement studies demonstrated that bGH and bPRL were both able to bind to this GHBP, because the bGH- and bPRL-binding activities of this protein could be saturated by an excess of either of these two hormones. This was indirectly confirmed by the close correlation (r = 0.615; P = 0.0001; n = 155) observed between plasma bGH- and bPRL-binding activities, because this correlation could suggest that both ligands are bound to the same proteins. The temporal concentrations of plasma GHBP were measured in samples collected at 20-min intervals for 24 hr from 8 young bulls. The evaluation of GHBP was realized by WLB, followed by densitometric analysis. Some fluctuations were observed, but these were not correlated with bGH release, even with a +/- 2-hr lag period. In summary, we found that bovine high-affinity GHBP binds not only bGH, but also bPRL. A second type of protein, of higher molecular weight, also binds these two hormones, but further investigations are needed to determine its nature. Finally, GHBP concentrations in cattle blood plasma apparently show fluctuations over a 24-hr period, but no correlation was found between these fluctuations and plasma growth hormone concentrations. [less ▲]

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See detailGrowth hormone, insulin-like growth factor-I and their plasma binding proteins in different cattle breeds.
Renaville, Robert ULg; Massart, Serge; Shojae, D. et al

in Zootecnica e Nutrizione Animale (1995), 21(2),

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See detailRole des somatomedines/insulin-like growth factors dans la croissance ponderale foetale.
Renaville, Robert ULg; Falaki, M.; Burny, A. et al

in Bulletin des Recherches Agronomiques de Gembloux (1994), 29(3),

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See detailEffects of immunization against somatostatin on growth performances and growth hormone - insulin-like growth factor I and insulin plasma levels in young bulls.
Renaville, Robert ULg; Sneyers, M.; Massart, Serge et al

in Zootecnica e Nutrizione Animale (1994), 20(4),

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See detailBovine Growth Hormone-Taqi Rflp And Quantitative Traits Breed Selected For Growth Performances And Meat Production
Sneyers, M.; Renaville, Robert ULg; Falaki, M. et al

in Archives Internationales de Physiologie, de Biochimie et de Biophysique (1994), 102(2),

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See detailTaqi Restriction-Fragment-Length-Polymorphisms For Growth-Hormone In Bovine Breeds And Their Association With Quantitative Traits
Sneyers, M.; Renaville, Robert ULg; Falaki, M. et al

in Growth Regulation (1994), 4(3),

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See detailChanges In The Hypophyseal-Gonadal Axis During The Onset Of Puberty In Young Bulls
Renaville, Robert ULg; Devolder, A.; Massart, Serge et al

in Journal of Reproduction and Fertility (1993), 99(2),

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See detailPresence Of Growth Hormone-Binding Proteins In Cattle Plasma And Milk
Devolder, A.; Renaville, Robert ULg; Sneyers, M. et al

in Journal of Endocrinology (1993), 138(1),

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See detailRegulation endocrinienne et manipulation de la croissance bovine
Renaville, Robert ULg; Sneyers, M.; Devolder, A. et al

in Cahiers Agricultures (1992), 1(2),

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See detailImportance de l'axe hypophyso-hepatique dans le regulation hormonale de la croissance chez le bovin.
Renaville, Robert ULg; Portetelle, Daniel ULg; Sneyers, M. et al

in Bulletin des Recherches Agronomiques de Gembloux (1991), 26(2),

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See detailCloning and characterization of a cDNA encoding the beta-subunit of the bovine insulin-like growth factor-1 receptor.
Sneyers, M.; Kettmann, Richard ULg; Massart, Serge et al

in DNA Sequence : The Journal of DNA Sequencing & Mapping (1991), 1(6),

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See detailCloning and characterization of a cDNA encoding the bovine insulin-like growth factor binding protein 1 (bIGFBP-1).
Sneyers, M.; Kettmann, Richard ULg; Massart, Serge et al

in DNA Sequence : The Journal of DNA Sequencing & Mapping (1991), 1(6),

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See detailApport de l'endocrinologie en selection laitiere bovine.
Renaville, Robert ULg; Massart, Serge; Shojae, D. et al

in Animal Genetic Resources Information (1991), (8),

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See detailEvolution des concentrations plasmatiques de testostérone et d'insulin-like growth factor I en période péripubertaire chez le bovin cathétérisé au niveau hépatique.
Renaville, Robert ULg; Berkans, X.; Portetelle, Daniel ULg et al

in Annales de Recherches Vétérinaires = Annals of Veterinary Research (1990), 21

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See detailProduction and Characterization of Monoclonal Antibodies to Bovid Herpesvirus-4
Dubuisson, J.; Thiry, Etienne ULg; Bublot, M. et al

in Veterinary Microbiology (1989), 19(4), 305-15

Thirty-five hybridoma cell lines secreting monoclonal antibodies (Mabs) against bovid herpesvirus-4 (BHV-4) strain V. Test were produced. These hybrid cells resulted from the fusion of SP2/0 myeloma cells ... [more ▼]

Thirty-five hybridoma cell lines secreting monoclonal antibodies (Mabs) against bovid herpesvirus-4 (BHV-4) strain V. Test were produced. These hybrid cells resulted from the fusion of SP2/0 myeloma cells with splenocytes of BALB/c mice previously immunized with purified BHV-4. A modified indirect fluorescent antibody test (IFAT) was applied as a screening procedure and was compared with an indirect enzyme-linked immunosorbent assay. The selected Mabs were tested by the same IFAT against a panel of BHV-4 field isolates and against bovine herpesvirus-1, bovine herpesvirus-2 and alcelaphine herpesvirus-1 (AHV-1). Comparison of BHV-4 field isolates with Mabs confirmed their close antigenic relationships, but slight antigenic differences were observed between different isolates. One of the Mabs also reacted against AHV-1, indicating an antigenic relationship between BHV-4 and AHV-1. None of the Mabs reacting with BHV-4 possessed neutralizing activity against the strain used for immunization. [less ▲]

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See detailCirculation de souches non cytopathogènes du virus BVD-MD dans des lots de taurillons
Waxweiler, Sophie; Karelle-Bui Thi, Lucie; Sneyers, M. et al

in Annales de Médecine Vétérinaire (1989), 133

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