References of "Sluysmans, Damien"
     in
Bookmark and Share    
See detailReal-time capture of the folding-unfolding transitions in a single oligorotaxane foldamer
Sluysmans, Damien ULg; Hubert, Sandrine ULg; Bruns, Carson et al

Scientific conference (2016, July)

Detailed reference viewed: 4 (2 ULg)
See detailReal-time capture of the folding-unfolding transitions in a single oligorotaxane foldamer
Sluysmans, Damien ULg; Stoddart, J. Fraser; Duwez, Anne-Sophie ULg

Conference (2016, May)

Detailed reference viewed: 9 (1 ULg)
See detailSingle molecule force spectroscopy on oligorotaxane foldamers
Sluysmans, Damien ULg; Stoddart, J. Fraser; Duwez, Anne-Sophie ULg

Conference (2016, April)

Detailed reference viewed: 3 (1 ULg)
Full Text
Peer Reviewed
See detailForce measurements reveal how small binders perturb the dissociation mechanisms of DNA duplex sequences
Burmistrova, Anastasia; Fresch, Barbara ULg; Sluysmans, Damien ULg et al

in Nanoscale (2016), 8

The force-driven separation of double-stranded DNA is crucial to the accomplishment of cellular pro- cesses like genome transactions. Ligands binding to short DNA sequences can have a local stabilizing or ... [more ▼]

The force-driven separation of double-stranded DNA is crucial to the accomplishment of cellular pro- cesses like genome transactions. Ligands binding to short DNA sequences can have a local stabilizing or destabilizing effect and thus severely affect these processes. Although the design of ligands that bind to specific sequences is a field of intense research with promising biomedical applications, so far, their effect on the force-induced strand separation has remained elusive. Here, by means of AFM-based single mole- cule force spectroscopy, we show the co-existence of two different mechanisms for the separation of a short DNA duplex and demonstrate how they are perturbed by small binders. With the support of Mole- cular Dynamics simulations, we evidence that above a critical pulling rate one of the dissociation pathways becomes dominant, with a dramatic effect on the rupture forces. Around the critical threshold, we observe a drop of the most probable rupture forces for ligand-stabilized duplexes. Our results offer a deep understanding of how a stable DNA–ligand complex behaves under force-driven strand separation [less ▲]

Detailed reference viewed: 56 (19 ULg)
See detailSingle molecule force spectroscopy on oligorotaxane foldamers
Sluysmans, Damien ULg; Hubert, Sandrine ULg; Bruns, Carson et al

Poster (2015, June)

Detailed reference viewed: 5 (1 ULg)
See detailSingle molecule force spectroscopy on oligorotaxane foldamers
Sluysmans, Damien ULg; Hubert, Sandrine ULg; Bruns, Carson et al

Poster (2015, April)

Detailed reference viewed: 5 (2 ULg)
See detailSingle molecule force spectroscopy on oligorotaxane foldamers
Sluysmans, Damien ULg; Stoddart, J. Fraser; Duwez, Anne-Sophie ULg

Conference (2015, January)

Detailed reference viewed: 2 (1 ULg)
Full Text
Peer Reviewed
See detailUnraveling the complexity of the interactions of DNA nucleotides with gold by single molecule force spectroscopy
Bano, Fouzia; Sluysmans, Damien ULg; Wislez, Arnaud ULg et al

in Nanoscale (2015), 7

Addressing the effect of different environmental factors on the adsorption of DNA to solid supports is critical for the development of robust miniaturized devices for applications ranging from biosensors ... [more ▼]

Addressing the effect of different environmental factors on the adsorption of DNA to solid supports is critical for the development of robust miniaturized devices for applications ranging from biosensors to next generation molecular technology. Most of the time, thiol-based chemistry is used to anchor DNA on gold – a substrate commonly used in nanotechnology – and little is known about the direct interaction between DNA and gold. So far there have been no systematic studies on the direct adsorption behavior of the deoxyribonucleotides (i.e., a nitrogenous base, a deoxyribose sugar, and a phosphate group) and on the factors that govern the DNA–gold bond strength. Here, using single molecule force spectroscopy, we investigated the interaction of the four individual nucleotides, adenine, guanine, cytosine, and thymine, with gold. Experiments were performed in three salinity conditions and two surface dwell times to reveal the factors that influence nucleotide–Au bond strength. Force data show that, at physiological ionic strength, adenine–Au interactions are stronger, asymmetrical and independent of surface dwell time as compared to cytosine–Au and guanine–Au interactions. We suggest that in these conditions only adenine is able to chemisorb on gold. A decrease of the ionic strength significantly increases the bond strength for all nucleotides. We show that moderate ionic strength along with longer surface dwell period suggest weak chemisorption also for cytosine and guanine. [less ▲]

Detailed reference viewed: 12 (1 ULg)
See detailSingle molecule force spectroscopy on oligorotaxane foldamers
Sluysmans, Damien ULg; Bruns, Carson; Zhu, Zhixue et al

Conference (2014, December)

Detailed reference viewed: 3 (1 ULg)
See detailSingle molecule force spectroscopy on oligorotaxane foldamers
Sluysmans, Damien ULg; Duwez, Anne-Sophie ULg

Scientific conference (2014, December)

Detailed reference viewed: 4 (2 ULg)
See detailSingle molecule force spectroscopy on oligorotaxane foldamers
Sluysmans, Damien ULg; Bruns, Carson; Zhu, Zhixue et al

Poster (2014, October)

Detailed reference viewed: 6 (1 ULg)
See detailSingle molecule force spectroscopy of oligorotaxane foldamers
Sluysmans, Damien ULg; Duwez, Anne-Sophie ULg

Scientific conference (2014, July)

Detailed reference viewed: 4 (1 ULg)
See detailSingle molecule force spectroscopy of oligorotaxane foldamers
Sluysmans, Damien ULg; Bruns, Carson; Zhu, Zhixue et al

Poster (2014, May)

Detailed reference viewed: 4 (1 ULg)
See detailSINGLE-MOLECULE AFM STUDY OF ADHESIVE POLYMERS PREPARED BY COBALT-MEDIATED RADICAL POLYMERIZATION AND NITRONE-MEDIATED RADICAL COUPLING
Willet, Nicolas ULg; Sluysmans, Damien ULg; Delvaux, Cédric ULg et al

Scientific conference (2012, September 10)

Well-defined poly(vinyl acetate) (PVAc) chains prepared by CMRP (cobalt-mediated radical polymerization) were coupled using an alkyne-functional nitrone via NMRC (nitrone-mediated radical coupling).1 In ... [more ▼]

Well-defined poly(vinyl acetate) (PVAc) chains prepared by CMRP (cobalt-mediated radical polymerization) were coupled using an alkyne-functional nitrone via NMRC (nitrone-mediated radical coupling).1 In all the cases, the coupling efficiencies were close to 90% or higher. The polymers mid-chain functionalized with an alkyne group were then reacted with azide-functionalized atomic force microscopy (AFM) tips via copper-catalyzed azide-alkyne cycloaddition (CuAAC). As a result, polymers having a double-branch architecture were linked to AFM tips via a short linker. The structure and the molecular parameters of the polymers were determined by NMR and GPC, whereas the ‘click’ step onto AFM tips was assessed by performing the same CuAAC reaction onto macroscopic surfaces and characterizing them by ATR FT-IR. The adhesive properties of these double-branched polymers were studied by AFM single-molecule force spectroscopy. By performing approach-retraction cycles in solution upon a glass surface, the interaction between single PVAc chains and the surface was investigated. The effect of the double-branch architecture on the adhesion forces was under focus. Setting a residence time of the tip on the surface before retraction was found to have a beneficial influence on the adhesion forces. Signs of multiple interactions acting in parallel were detected in the experimental force-distance traces. [less ▲]

Detailed reference viewed: 134 (33 ULg)