References of "Scippo, Marie-Louise"
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See detailGuiana Dolphins (Sotalia guianensis) and DR-CALUX for Screening Coastal Brazilian Environments for Dioxins and Related Compounds
Dorneles, Paulo; Lailson-Brito, Jose; Bisi, Tatiana et al

in Archives of Environmental Contamination & Toxicology (2016)

Guiana dolphin is the top predator of highest toxicological concern in Brazil and many studies on levels of persistent, bioaccumulative, and toxicant (PBT) pollutants have been performed on the species ... [more ▼]

Guiana dolphin is the top predator of highest toxicological concern in Brazil and many studies on levels of persistent, bioaccumulative, and toxicant (PBT) pollutants have been performed on the species. However, due to high costs of the analyses, only one investigation comprised the determination of dioxins and related compounds (DRCs) in Guiana dolphin tissues. The dioxin responsive-chemically activated luciferase gene expression (DR-CALUX ) cell bioassay was used in the present study for the analyses of hepatic samples from 28 male Guiana dolphins in order to screen estuarine environments for DRCs, comprising three regions (Northeastern, Southeastern, and Southern) and four states [Parana´ (PR), Rio de Janeiro (RJ), Espı´rito Santo (ES), and Ceara´ (CE)] of Brazil. High bioanalytical equivalent (BEQ) concentrations [dioxins (pg BEQ/g lipid)] were found, varying from 1.94 to 15.6 pg BEQ/g. A significant negative correlation between BEQ concentrations and total length was found in Guiana dolphins from Brazil (all analysed dolphins). This pattern also was verified for RJ state, pointing to (1) chemically induced developmental disruption or to (2) increasing efficiency of the detoxifying activity with the growth of the animal. Comparison was performed with literature data and significantly higher BEQ levels were found in Brazilian Guiana dolphins than in those reported for North Sea harbour porpoises. Higher levels were found in Southeastern (the most PBT-contaminated area of the country) than in Southern region. However, it is not possible to affirm that Guiana dolphins are more contaminated by DRCs in SE than in S region, because individuals were lengthier in S than in SE region. Our results seem to have mirrored dolphin exposure to PCBs in Brazil according to the literature. Further studies are required for investigating the hypotheses 1 and 2 mentioned above. [less ▲]

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See detailAssessment of methods of detection of water estrogenicity for their use as monitoring tools in a process of estrogenicity removal
Blavier; Songulashvili; Simon, Coraline ULg et al

in Environmental Technology (2016)

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See detailLinseed oil presents different patterns of oxidation in real-time and accelerated aging assays
Douny, Caroline ULg; Razanakolona, Rina; Ribonnet, Laurence et al

in Food Chemistry (2016), 208

This study aimed at verifying if the hypothesis that one day at 60 °C is equivalent to one month at 20 °C could be confirmed during linseed oil aging for 6 months at 20 °C and 6 days at 60 °C using the ... [more ▼]

This study aimed at verifying if the hypothesis that one day at 60 °C is equivalent to one month at 20 °C could be confirmed during linseed oil aging for 6 months at 20 °C and 6 days at 60 °C using the “Schaal oven stability test”. Tests were conducted with linseed oil supplemented or not with myricetin or butyl-hydroxytoluene as antioxidants. Oxidation was evaluated with the peroxide and p-anisidine values, as well as the content in conjugated dienes and aldehydes. All four indicators of oxidation showed very different kinetic behaviors at 20 and 60 °C. The hypothesis is thus not verified for linseed oil, supplemented or not with antioxidant. In the control oil, the conjugated dienes and the peroxide value observed were respectively of 41.8 ± 0.8 Absorbance Unit (AU)/g oil and 254.3 ± 5.8 meq. O2/kg oil after 6 months at 20 °C. These values were of 18.2 ± 1.3 AU/g oil and 65.2 ± 20.3 meq. O2/kg after 6 days at 60 °C. [less ▲]

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See detailInvestigation of the genotoxicity of substances migrating from polycarbonate replacement baby bottles to identify chemicals of high concern.
Mertens; Simon, Coraline ULg; Van Bossuyt et al

in Food & Chemical Toxicology (2016), 89

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See detailReassessment of the acrylamide risk: Belgium as a case-study
Claes, Wendie; De Meulenaer, Bruno; Huyghebaert, André et al

in Food Control (2016), 59

Acrylamide is a food process contaminant with carcinogenic and genotoxic properties. As a result of intensive research, numerous mitigation initiatives to prevent its formation were suggested and various ... [more ▼]

Acrylamide is a food process contaminant with carcinogenic and genotoxic properties. As a result of intensive research, numerous mitigation initiatives to prevent its formation were suggested and various of them were implemented in the food chain. To evaluate if the mitigation strategies applied were significant, a comparison was made between two time periods (2002e2007 versus 2008e2013) in terms of acrylamide food levels and dietary exposure in Belgian. The most important changes observed are a significant decrease of the acrylamide content in potato crisps and gingerbread, and a significant increase in (instant) coffee. Additionally, the acrylamide content of breakfast cereals, bread and rolls, chocolate and baby biscuits showed a downward trend, whereas for coffee substitute and ready-to-eat French fries (mainly obtained from catering facilities), an upward, although not significant, trend was observed. These changes resulted in only a slight, but insignificant decline of the overall dietary exposure of adults, adolescents and children. The mean and P95 intake estimated in the 2008e2013 period for these consumer groups corresponded to margins of exposure (MOE) ranging between 515 and 236 and between 155 and 71, respectively, when based on the endpoint for neoplastic effects (BMDL10 ¼ 0.17 mg/kg bw per day). Such low MOE values indicate that acrylamide remains an issue for public concern, requiring renewed attention. [less ▲]

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See detailMulti-allergens detection by flow cytometric immunoassay
Otto; Planque; Lamote et al

Poster (2015, November)

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See detailTests in vitro pour l’évaluation des substituts du Bisphenol A
Simon, Coraline ULg; Scippo, Marie-Louise ULg

Scientific conference (2015, May 18)

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See detailEffect of storage and cooking on the fatty acid profile of omega-3 enriched eggs and pork meat marketed in Belgium
Douny, Caroline ULg; El Khoury, Rawad; Delmelle, Julien et al

in Food Science & Nutrition (2015), 3(2), 140-152

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See detailMonitoring Antibiotic Use and Residue in Freshwater Aquaculture for Domestic Use in Vietnam
Pham Kim, Dang; Chu, Jacqueline; Nga Thuy, Do et al

in EcoHealth (2015)

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See detailQuality of Antimicrobial Products Used in Striped Catfish (Pangasianodon hypophthalmus) Aquaculture in Vietnam.
Tran Minh Phu; Nguyen Than Phuong; Scippo, Marie-Louise ULg et al

in PLoS ONE (2015), 10(4): e0124267. doi:10.1371/journal.pone.0124267

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See detailEstrogenic Evaluation and Organochlorine Identification in Blubber of North Sea Harbour Porpoise (Phocoena phocoena) Stranded on the North Sea Coast
Didimo Imazaki, Pedro Henrique ULg; Brose, François ULg; Jauniaux, Thierry ULg et al

in BioMed Research International (2015), Volume 2015(Article ID 438295), 13

Thirteen individual organochlorine compounds at 3 concentrations (80, 400, and 2000 ng/mL culture medium), as well as mixtures, were assayed for the estrogen receptor (ER) activation or inhibition, using ... [more ▼]

Thirteen individual organochlorine compounds at 3 concentrations (80, 400, and 2000 ng/mL culture medium), as well as mixtures, were assayed for the estrogen receptor (ER) activation or inhibition, using a luciferase reporter gene assay (RGA). None of the PCB 138, 153, or 180 or their mixture induced a response in the RGA. o,p'-DDT was the most potent xenoestrogen fromthe DDT group, inducing a response already at 80 ng/mL. From the HCH and HCB group, only 𝛽-HCH (at 400 and 2000 ng/mL) and 𝛿-HCH (at 2000 ng/mL) displayed estrogenic activities.These 13 organochlorines were determined by GC-MS in 12 samples of North Sea harbor porpoise blubber. The PCBs were the main contaminants. Within each group, PCB 153 (6.0 × 102∼4.2 × 104 𝜇g/kg), p,p'- DDE (5.1 × 102∼8.6 × 103 𝜇g/kg), and HCB (7.6 × 101∼1.5 × 103 𝜇g/kg) were the compounds found in highest concentrations.The hormonal activity of the porpoise blubber samples was also assayed in RGA, where two samples showed estrogenic activity, seven samples showed antiestrogenic activity, and one sample showed both estrogenic and antiestrogenic activity. Our results suggest that the 13 POPs measured by GC-MS in the samples cannot explain alone the estrogenicity of the extracts. [less ▲]

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See detailImpact of aging technique, muscle and previous vacuum storage time on oxidative stability of beef packaged under high-oxygen atmosphere
Didimo Imazaki, Pedro Henrique ULg; Douny, Caroline ULg; Scippo, Marie-Louise ULg et al

Conference (2014, December 11)

The aim of this study was to compare the effect of aging technique (wet-aging vs. carcass-aging), muscle (longissimus dorsi vs. rectus femoris) and previous vacuum storage time on color and lipid ... [more ▼]

The aim of this study was to compare the effect of aging technique (wet-aging vs. carcass-aging), muscle (longissimus dorsi vs. rectus femoris) and previous vacuum storage time on color and lipid stability of beef packaged in high-oxygen atmosphere. After a seven-day wet- or carcass-aging step, longissimus dorsi and rectus femoris muscle cuts from 4 Belgian Blue cows were vacuum packaged and stored at −1 °C for up to 28 days. At different times, part of these samples was repackaged under modified atmosphere – 70 % O2:30 % CO2 –, and stored during 7 days at +4 °C. The following parameters were evaluated at different intervals: color (CIE L*a*b*), metmyoglobin %, lipid oxidation (TBARS), antioxidant enzyme activities (catalase, glutathione peroxidase and superoxide dismutase), alpha-tocopherol and fat content. The sensitivity of modified atmosphere repacked meat cuts to oxidation was influenced by the conditions of the previous aging period (wet > carcass conditions), muscle (rectus femoris > longissimus dorsi) and length of the vacuum storage. Oxidation stability could be associated with catalase activity, and no association could be established with the alpha-tocopherol content. [less ▲]

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See detailChemical Food Safety of Aquaculture Products from South-East Asia
Scippo, Marie-Louise ULg; Kestemont, Patrick

Conference (2014, December 04)

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See detailIn vitro study toward the endocrine activity and the genotoxic potential of migration products from plastic baby bottles
Simon, Coraline ULg; Onghena, M.; Covaci, A. et al

Poster (2014, November 20)

Bisphenol A (BPA) is used since 1960 as a primary raw material for the production of polycarbonate (PC) plastic and epoxy resin, widely used in a variety of common products including digital media (e.g ... [more ▼]

Bisphenol A (BPA) is used since 1960 as a primary raw material for the production of polycarbonate (PC) plastic and epoxy resin, widely used in a variety of common products including digital media (e.g., CDs, DVDs), electrical and electronic equipment, automobiles, sports safety equipment, reusable food and drink containers , as well as baby bottle. During the last decades, in several studies, the migration of BPA is documented to be a well-known source of food contamination. Some studies have shown that BPA, which is able to mimic the action of and hormone and to disrupt normal endocrine function, may be associated to several health problems and diseases. Recently, the European Union took a series of measures, including a ban for the import and sale PC baby bottles to reduce BPA exposure to infants. Plastic alternatives to polycarbonate have massively appeared on Belgium market. Although there are several studies on BPA migration from polycarbonate into foodstuff under a variety of conditions, there is a small amount of information about consequences on human health of the potential exposure to chemicals migrating from PC alternatives, including bottles commonly labelled “free BPA”. In a recent opinion (No. 8697, 11.03.2010), the Belgium Superior Health Council's issued its concern regarding the currently used alternatives to PC. Furthermore, they asked to investigate the possible risks associated with the use of these alternatives. In order to evaluate the safety of these alternatives, the genotoxicity and the activity on several receptors of chemical compounds migrating from PC alternatives, identified by Simoneau & al, 2012 , were evaluated using reporter gene assays. Receptor agonistic and antagonistic activities of 39 pure compounds were measured. After the first screening, certain substances clearly showed an activity on several receptors such as BPA, and Bisphenol S, while only a few substances showed no reaction on the different receptor. None of the 39 components was genotoxic as identified in the Vitotox test. However, further experiments will be performed to characterize their activity and confirm the result for the genotoxicity. [less ▲]

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See detailLes perturbateurs endocriniens : ce n'est pas la dose qui fait le poison
Maghuin-Rogister, Guy ULg; Scippo, Marie-Louise ULg

Conference given outside the academic context (2014)

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See detailStabilité oxydative des viandes blanc bleu belge conditionnées sous atmosphère riche en oxygène après maturation sous vide ou en carcasse
Didimo Imazaki, Pedro Henrique ULg; Douny, Caroline ULg; Scippo, Marie-Louise ULg et al

in Viandes et Produits Carnés (2014, November)

The aim of this study was to evaluate the physico-chemical stability of Belgian Blue beef packaged under high-oxygen atmosphere as a function of a previous aging technique (wet-aging in vacuum conditions ... [more ▼]

The aim of this study was to evaluate the physico-chemical stability of Belgian Blue beef packaged under high-oxygen atmosphere as a function of a previous aging technique (wet-aging in vacuum conditions vs. carcass-aging). Two muscles (longissimus dorsi vs. rectus femoris) were studied. After a seven-day wet- or carcass-aging step, muscle cuts from 4 Belgian Blue cows were vacuum packaged and stored at −1 °C for up to 28 days. Each 14 days, part of these samples was repackaged under modified atmosphere (70 % O2:30 % CO2), and stored during 7 days at +4 °C. The following parameters were evaluated: color, metmyoglobin %, metmyoglobin reducing activity, fat content, fatty acid profile, lipid oxidation, antioxidant enzyme activities and alpha-tocopherol content. The sensitivity of modified atmosphere repacked meat cuts to oxidation was influenced by the conditions of the previous aging period (wet > carcass conditions), muscle (rectus femoris > longissimus dorsi) and length of the vacuum storage. [less ▲]

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