 Calcium phosphate scaffolds customizations for bone tissue engineering applicationsCarlier, Aurélie  ; ; et alPoster (2011, November 18) Detailed reference viewed: 13 (3 ULg)An integrative model based approach to optimize calcium phosphate scaffold-stem cell combinationsCarlier, Aurélie ; ; et alPoster (2011, June 07) Detailed reference viewed: 5 (0 ULg)Optimization of calcium phosphate scaffold-cell combinations using an integrative model based approachCarlier, Aurélie ; ; et al(2011, June 03) Detailed reference viewed: 8 (2 ULg) The combined bone forming capacity of human periosteal derived cells and calcium phosphates.; Geris, Liesbet ; et alin Biomaterials (2011), 32(19), 4393-405 Current knowledge suggests that the periosteum, a fibrous tissue which covers the surface of all bones, contains a population of progenitor cells which mediate the repair of bone defects. In an effort to ... [more ▼] Current knowledge suggests that the periosteum, a fibrous tissue which covers the surface of all bones, contains a population of progenitor cells which mediate the repair of bone defects. In an effort to optimise the utilisation of this source of cells for bone engineering, herein we describe the rational selection of calcium phosphate (CaP) containing materials, based on biomaterial properties, and evaluation of their combined bone forming capacity. Five different commercially available orthopaedic 3D matrices composed of CaP particles in an open collagen network (NuOss, CopiOs, Bio-Oss((R)), Collagraft and Vitoss((R))) were evaluated in vitro and in vivo with human periosteal derived cells (hPDCs). It was found that the cell-material combinations behaved quite differently in vivo, despite apparent in vitro similarities in gene expression profiles. Bone formation was highest within the NuOss/hPDC implant at 13.03%, which also contained the highest incidence of bone marrow formation. The bone formed in this implant was chimeric with approximately 65% originating from implanted cells. Upon analysis of human specific gene expression, although it was found that predominantly osteogenic differentiation was observed within NuOss/hPDC implants, a lesser induction of chondrogenic genes was also observed. The formation of a cartilage intermediate was confirmed by histology. Additionally the NuOss/hPDC implant integrated into the mouse environment with apparent active scaffold resorption. This study demonstrates the importance of matching a cell support/biological matrix with a cell type and subsequently has outlined parameters which can be used for the rational selection of biomaterials for bone engineering. [less ▲] Detailed reference viewed: 15 (0 ULg)Computational modelling of calcium mediated bone regenerationCarlier, Aurélie ; ; et alPoster (2010, November 26) Detailed reference viewed: 8 (1 ULg)A mathematical model of calcium ion influence on the activity of osteogenic cellsCarlier, Aurélie ; ; et alPoster (2010, November 19) Detailed reference viewed: 4 (0 ULg)A mathematical model of calcium ion influence on the activity of osteogenic cellsCarlier, Aurélie ; ; et alPoster (2010, November 19) Detailed reference viewed: 7 (0 ULg)Experimentally-informed Mathematical Modelling of Oxygen Tension, Cell Viability and Proliferation in Fibrin Hydrogels; ; Geris, Liesbet et alin Proceedings of the TERMIS-EU meeting (2010, June) Detailed reference viewed: 5 (0 ULg)Towards a quantitative understanding of oxygen tension and cell density evolution in fibrin hydrogels; ; Geris, Liesbet et alin Biomaterials (2010), 32(1), 107-118 The in vitro culture of hydrogel-based constructs above a critical size is accompanied by problems of unequal cell distribution when diffusion is the primary mode of oxygen transfer. In this study, an ... [more ▼] The in vitro culture of hydrogel-based constructs above a critical size is accompanied by problems of unequal cell distribution when diffusion is the primary mode of oxygen transfer. In this study, an experimentally informed mathematical model was developed to relate cell proliferation and death inside fibrin hydrogels to the local oxygen tension in a quantitative manner. The predictive capacity of the resulting model was tested by comparing its outcomes to the density, distribution and viability of human periosteum derived cells (hPDCs) that were cultured inside fibrin hydrogels in vitro. The model was able to reproduce important experimental findings, such as the formation of a multilayered cell sheet at the hydrogel periphery and the occurrence of a cell density gradient throughout the hydrogel. In addition, the model demonstrated that cell culture in fibrin hydrogels can lead to complete anoxia in the centre of the hydrogel for realistic values of oxygen diffusion and consumption. A sensitivity analysis also identified these two parameters, together with the proliferation parameters of the encapsulated cells, as the governing parameters for the occurrence of anoxia. In conclusion, this study indicates that mathematical models can help to better understand oxygen transport limitations and its influence on cell behaviour during the in vitro culture of cellseeded hydrogels. [less ▲] Detailed reference viewed: 12 (0 ULg) |
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