References of "Schrooten, Jan"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailA MODEL FOR CELL/MATRIX GROWTH ON 3D SURFACES: A COUPLING OF LEVEL SET METHOD AND BRINKMAN EQUATION
Guyot, Yann ULg; Papantoniou, Ioannis; Chai, Yoke Chin et al

Conference (2013, September 11)

Detailed reference viewed: 49 (4 ULg)
Full Text
Peer Reviewed
See detailMULTIPHYSICS MODELING OF CELL/MATRIX GROWTH ON 3D STRUCTURES.
Guyot, Yann ULg; Papantoniou, Ioannis; Chai, Yoke Chin et al

Conference (2013, August 26)

Detailed reference viewed: 31 (2 ULg)
Full Text
Peer Reviewed
See detailA MATHEMATICAL MODEL FOR CELL/MATRIX GROWTH ON 3D SURFACES USING THE LEVEL SET METHOD.
Guyot, Yann ULg; Papantoniou, Ioannis; Chai, Yoke Chin et al

Conference (2013, April 03)

Detailed reference viewed: 19 (2 ULg)
Peer Reviewed
See detailIn vitro and in vivo comparative study of material properties crucial for reverse engineering of calcium phosphate scaffolds
Chai, Yoke Chin; Kerckhofs, Greet ULg; Bertels, Jeroen et al

Conference (2013)

Detailed reference viewed: 6 (0 ULg)
Peer Reviewed
See detail3D perfusion bioreactor culture of human periosteum derived stem cells for bone tissue engineering
Sonnaert, Maarten; Papantoniou, Ioannis; Bloemen, Veerle et al

Conference (2013)

Detailed reference viewed: 2 (1 ULg)
Peer Reviewed
See detailContrast-enhanced nanofocus computed tomography for virtual 3D histopathology and morphometric analysis of multiple skeletal tissues
Kerckhofs, Greet ULg; Papantoniou, Ioannis; Sonnaert, Maarten et al

Conference (2013)

Detailed reference viewed: 6 (2 ULg)
Peer Reviewed
See detailMicro-CT for first line screening of the scaffold material-, cell-and donor-variability on the ectopic bone forming capacity of tissue engineering constructs
Geeroms, Carla; Roberts, Scott; Van Hove, Astrid et al

Conference (2013)

Detailed reference viewed: 1 (1 ULg)
Peer Reviewed
See detailOptimised contrast enhanced nanoCT for volumetric analyses of in vitro manufactured tissue-engineered bone constructs
Sonnaert, Maarten; Kerckhofs, Greet ULg; Papantoniou, Ioannis et al

Conference (2013)

Detailed reference viewed: 4 (1 ULg)
Peer Reviewed
See detailContrast enhanced nanoCT for volumetric analyses of in vitro manufactured tissue-engineered bone constructs: a soft tissue case study
Sonnaert, Maarten; Papantoniou, Ioannis; Geris, Liesbet ULg et al

Conference (2013)

Detailed reference viewed: 2 (1 ULg)
Peer Reviewed
See detailContrast enhanced nanoCT for 3D quantitative and spatial analysis of in vitro manufactured extracellular matrix in metallic tissue engineering scaffolds
Sonnaert, Maarten; Papantoniou, Ioannis; Geris, Liesbet ULg et al

in Abstract book User Meeting Bruker MicroCT 2013 (2013)

Detailed reference viewed: 3 (1 ULg)
Full Text
Peer Reviewed
See detailSurface Roughness and Morphology Customization of Additive Manufactured Open Porous Ti6Al4V Structures
Pyka, Grzegorz; Kerckhofs, Greet ULg; Papantoniou, Ioannis et al

in Materials (2013), 6(10), 4737-4757

Detailed reference viewed: 4 (0 ULg)
Peer Reviewed
See detailMicro-CT evaluation of the effect of material, donor and implantation site variability on the bone forming capacity of progenitor cell/CaP-collagen constructs implanted ectopically in nude mice
Van Hove, Astrid; Geeroms, Carla; Maréchal, Marina et al

in Abstract book User Meeting Bruker MicroCT 2013 (2013)

Detailed reference viewed: 5 (0 ULg)
Full Text
Peer Reviewed
See detailThree dimensional characterization of tissue-engineered constructs by contrast enhanced nanofocus computed tomography.
Papantoniou, Ioannis; Sonnaert, Maarten; Geris, Liesbet ULg et al

in Tissue engineering. Part C, Methods (2013)

In order to successfully implement tissue engineered (TE) constructs as part of a clinical therapy, it is necessary to first develop and validate quality control tools that will ensure accurate and ... [more ▼]

In order to successfully implement tissue engineered (TE) constructs as part of a clinical therapy, it is necessary to first develop and validate quality control tools that will ensure accurate and consistent TE construct release specifications. Hence advanced methods to monitor TE construct properties need to be further developed. In this study we showed proof of concept for contrast enhanced nanofocus computed tomography (CE-nanoCT) as a 'whole-construct' imaging technique with non-invasive potential that enables 3D visualization and quantification of in vitro engineered extracellular matrix (ECM) in TE constructs. In particular we performed a 3D quantitative and qualitative structural and spatial assessment of the in vitro engineered ECM, formed during static and perfusion bioreactor cell culture in 3D TE scaffolds, using two contrast agents, namely Hexabrix(R) and phosphotungstic acid (PTA). CE-nanoCT image data were validated by comparison to Live/Dead viability/cytotoxicity and picrosirius red staining data, and to the net dry weight of the TE constructs. When using Hexabrix(R) as contrast agent, ECM volume fitted linearly with net dry ECM weight independent from the flow rate used. When using PTA as contrast agent, CE-nanoCT data showed pronounced distinction between flow conditions when compared to both net dry weight and picrosirius red staining data although linearity was maintained, indicating culture-specific structural ECM differences. This was attributed to the binding specificity of this contrast agent. This novel type of information can contribute to optimize bioreactor culture conditions and potentially critical quality characteristics of TE constructs such as ECM quantity and homogeneity, facilitating the gradual transformation of 'TE constructs' in well characterized 'TE products'. [less ▲]

Detailed reference viewed: 9 (2 ULg)
Full Text
Peer Reviewed
See detailValidation of a finite element model of a unilateral external fixator in a rabbit tibia defect model.
Karunratanakul, Kavin; Kerckhofs, Greet ULg; Lammens, Johan et al

in Medical engineering & physics (2013), 35(7), 1037-43

In case of large segmental defects in load-bearing bones, an external fixator is used to provide mechanical stability to the defect site. The overall stiffness of the bone-fixator system is determined not ... [more ▼]

In case of large segmental defects in load-bearing bones, an external fixator is used to provide mechanical stability to the defect site. The overall stiffness of the bone-fixator system is determined not only by the fixator design but also by the way the fixator is mounted to the bone. This stiffness is an important factor as it will influence the biomechanical environment to which tissue engineering scaffolds and regenerating tissues are exposed. A finite element (FE) model can be used to predict the system stiffness. The goal of this study is to develop and validate a 3D anatomical FE model of a bone-fixator system which includes a previously developed unilateral external fixator for a large segmental defect model in the rabbit tibia. It was hypothesized that the contact interfaces between bone and fixator screws play a major role for the prediction of the stiffness. In vitro mechanical testing was performed in order to measure the axial stiffness of cortical bone from mid-shaft rabbit tibiae and of the tibia-fixator system, as well as the bending stiffness of individual fixator screws, inserted in bone. muCT-based case-specific FE models of cortical bone and SCREW-BONE specimens were created to simulate the corresponding mechanical test set-ups. The Young's modulus of rabbit cortical bone as well as appropriate screw-bone contact settings were derived from those FE models. We then used the derived settings in an FE model of the tibia-fixator system. The difference between the FE predicted and measured axial stiffness of the tibia-fixator system was reduced from 117.93% to 7.85% by applying appropriate screw-bone contact settings. In conclusion, this study shows the importance of screw-bone contact settings for an accurate fixator stiffness prediction. The validated FE model can further be used as a tool for virtual mechanical testing in the design phase of new tissue engineering scaffolds and/or novel patient-specific external fixation devices. [less ▲]

Detailed reference viewed: 8 (1 ULg)
Full Text
Peer Reviewed
See detailFluorescent oxygen sensitive microbead incorporation for measuring oxygen tension in cell aggregates.
Lambrechts, Dennis; Roeffaers, Maarten; Kerckhofs, Greet ULg et al

in Biomaterials (2013), 34(4), 922-9

Molecular oxygen is a main regulator of various cell functions. Imaging methods designed as screening tools for fast, in situ, 3D and non-interfering measurement of oxygen tension in the cellular ... [more ▼]

Molecular oxygen is a main regulator of various cell functions. Imaging methods designed as screening tools for fast, in situ, 3D and non-interfering measurement of oxygen tension in the cellular microenvironment would serve great purpose in identifying and monitoring this vital and pivotal signalling molecule. We describe the use of dual luminophore oxygen sensitive microbeads to measure absolute oxygen concentrations in cellular aggregates. Stable microbead integration, a prerequisite for their practical application, was ensured by a site-specific delivery method that is based on the interactions between streptavidin and biotin. The spatial stability introduced by this method allowed for long term measurements of oxygen tension without interfering with the cell aggregation process. By making multiple calibration experiments we further demonstrated the potential of these sensors to measure local oxygen tension in optically dense cellular environments. [less ▲]

Detailed reference viewed: 6 (0 ULg)
Full Text
Peer Reviewed
See detailHigh-Resolution Microfocus X-Ray Computed Tomography for 3D Surface Roughness Measurements of Additive Manufactured Porous Materials
Kerckhofs, Greet ULg; Pyka, Grzegorz; Moesen, Maarten et al

in ADVANCED ENGINEERING MATERIALS (2013), 15(3), 153-158

Since commercially available profiling systems fail when determining the surface roughness of porous materials, we propose a novel protocol for surface roughness quantification of 3D additive manufactured ... [more ▼]

Since commercially available profiling systems fail when determining the surface roughness of porous materials, we propose a novel protocol for surface roughness quantification of 3D additive manufactured porous structures based on high-resolution microfocus X-ray computed tomography (micro-CT) images. It allows to non-destructively assess the roughness of these porous materials at the outer surface as well as inside the structure. The noise in the images and the spatial image resolution both have a significant effect on the accuracy of the micro-CT-based roughness measurements. Comparing the roughness parameters of flat substrates determined both with commercially available (optical and contact) profiling systems and the micro-CT-based roughness measurement protocol shows that micro-CT can be applied accurately and in a robust manner for surface roughness quantification of 3D additive manufactured porous materials with a micro-scale roughness. Depending on the dimensions of the roughness, the micro-CT acquisition parameters, i.e., frame averaging and spatial image resolution, need to be fine-tuned. Submicron-scale roughness can currently not be quantified. [less ▲]

Detailed reference viewed: 14 (4 ULg)
Full Text
Peer Reviewed
See detailMathematical Level-Set Modelling of Cell Growth on 3D Surfaces
Guyot, Yann ULg; Papatoniou, Ioannis; Chai, Yoke Chin et al

Poster (2012, September)

Detailed reference viewed: 17 (6 ULg)
Peer Reviewed
See detailHigh-resolution non-destructive 3D quantitative imaging of the cartilage subarchitecture
Kerckhofs, Greet ULg; Sainz, Julie; Wevers, Martine et al

Conference (2012)

Detailed reference viewed: 7 (2 ULg)
Peer Reviewed
See detailµCT-Based, Anatomical Finite Element Model of a Unilateral Fixator for Bone Reconstruction in Rabbit Tibia
Karunratanakul, Kavin; Kerckhofs, Greet ULg; Lammens, Johan et al

Conference (2012)

Detailed reference viewed: 7 (1 ULg)