References of "Sandersen, Charlotte"
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See detailPhysiological Responses to training and competition in 1 to 4 star eventing horses
Kirsch, Katharina ULg; Düe, M.; Holzhausen, H. et al

Poster (2016, October 21)

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See detailReported post-operative analgesic administration to non-human primates: 2010 - 2015
Bertrand, Henri ULg; Flecknell, Paul; Brady, James et al

Poster (2016, October 21)

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See detailComputed tomography-guided injection of muscle-derived mesenchimal stem cells in the intervertebral disc of dogs affected by natural disc degeneration: clinical safety and intervertebral disc imaging assessment
Liotta, Annalisa Pia ULg; Sandersen, Charlotte ULg; Ceusters, Justine ULg et al

Poster (2016, September 01)

Introduction/Purpose: Pre-clinical randomized controlled animal trials have been conducted to evaluate the effects of mesenchymal stem cell (MSCs) transplantation on intervertebral disc (IVD) degeneration ... [more ▼]

Introduction/Purpose: Pre-clinical randomized controlled animal trials have been conducted to evaluate the effects of mesenchymal stem cell (MSCs) transplantation on intervertebral disc (IVD) degeneration. According to their histological results and to imaging assessment of the IVD, intradiscal injection of MSCs is effective, arresting or slowing IVDD process, and is associated with a low complication rate. Few of these studies have been conducted on canine artificially degenerated IVD, using bone marrow or adipose-derived MSCs. Therefore a systematic study on naturally degenerated IVD using MSCs obtained from autologous muscular tissue in dogs is still lacking. The aims of this study were to evaluate the clinical effects of intradiscal injection of muscle-derived MSCs and its effects on imaging features of the intervertebral disc. Methods: Eight experimental dogs were randomly included with the approval of the University’s Animal Care and Use Committee. The final inclusion criteria were the presence of naturally degenerated lumbosacral IVD detected on low-field magnetic resonance (MRI) images and the obtaining of 3 x 106 autologous muscle-derived MSCs. A computed tomography (CT) and MRI examination was performed before and 2 months after the procedure and 13 imaging parameters were assessed. Mesenchymal stem cells diluted in 0,2 ml of FRS Hypothermosol were injected in the lumbosacral IVD under CT-guidance. Clinical examinations were performed regularly during 1 month after the procedure. Results: Six dogs met the inclusion criteria. The remaining 2 dogs did not undergo intradiscal injections, but were used as control group. No major or minor complications were reported during the procedure. No abnormalities were noticed during the clinical examinations. No statistically significant variations of IVD imaging features were noticed before and after the injections. Discussion/Conclusions: Intradiscal injection of muscle-derived MSCs is clinically safe and it is not associated with any progression of the IVD degeneration, detected by CT or low-field MRI imaging. Further studies are needed to assess its efficacy as treatment for the canine natural IVD degeneration. [less ▲]

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See detailMitochondrial function and aerobic capacity assessed by high resolution respirometry in Thoroughbred horses
Serteyn, Didier ULg; Ceusters, Justine ULg; Nonnenmacher et al

in Comparative Exercise Physiology (2016), 12(2), 67-73

During the initial stages of training of young Thoroughbred horses, low intensity exercise is employed to increase aerobic capacity. High Resolution Respirometry (HRR) allows the determination of aerobic ... [more ▼]

During the initial stages of training of young Thoroughbred horses, low intensity exercise is employed to increase aerobic capacity. High Resolution Respirometry (HRR) allows the determination of aerobic capacities in small samples of permeabilised muscle fibres. The aim of the study was to measure the mitochondrial function by HRR in Thoroughbred horses, to compare these values to Warmblood horses and to evaluate the effect of a 10-weeks training period. The mitochondrial function was measured by HRR using different substrate-uncoupler protocols (SUIT 1 and 2) in muscle microbiopsies from two groups of untrained horses: 17 Warmblood and 8 Thoroughbred and in the group of 8 Thoroughbred horses before and after a 10-week training period. The SUIT1 protocol employed to compare the two groups of horses showed that in Thoroughbred horses, the mean values for oxygen flux expressed as tissue mass-specific respiration were significantly higher for complex I (CI)Glutamate+Malate, CI + complex II, and maximum electron transport capacities (ETSmax) than the mean values measured in Warmblood horses. The SUIT 1 and SUIT 2 protocols revealed large differences among Thoroughbred horses before and after training. The SUIT 2 protocols showed a significant difference for the complex I activity before and after training but only when the oxygen flux was expressed as percentage of ETSmax. This study shows the interest of HRR in equine sport medicine and exercise physiology, but shows that the technique requires further refinement. Indeed significant differences have been shown between the Thoroughbred and the Warmblood horses highlighting the need to have baseline data for each breed. The Thoroughbred horses had globally a high oxidative phosphorylation capacity with an increase of CI activity induced by an aerobic training program. [less ▲]

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See detailPhysiological response to training and competition in 1-star to 4-star eventing horses
KIRSCH, Katharina; Düe, Michael; Holzhausen, Hermann et al

Poster (2016, June)

Abstract 034 – Physiological response to training and competition in 1-star to 4-star eventing horses – Post Review Authors’ names and affiliations Author 1: Katharina Kirsch* (German Olympic Committee ... [more ▼]

Abstract 034 – Physiological response to training and competition in 1-star to 4-star eventing horses – Post Review Authors’ names and affiliations Author 1: Katharina Kirsch* (German Olympic Committee for Equestrian Sports, Germany and PhD Student, University of Liège, Belgium) Author 2: Michael Düe (German Equestrian Federation, Germany) Author 3: Hermann Holzhausen (Olympic Support Center Westphalia – Warendorf, Germany) Author 4: Stephanie Horstmann (German Olympic Committee for Equestrian Sports, Germany) Author 5: Markus Scharmann (German Equestrian Federation, Germany) Author 6: Charlotte Sandersen (University of Liège, Belgium) *Address of the presenting author: Katharina Kirsch, Freiherr-von-Langen-Str. 15, 48231 Warendorf, GERMANY E-mail of the presenting author: kkirsch@fn-dokr.de Abstract Physiological response to training and competition in 1-star to 4-star eventing horses To prepare horses for the requirements of international eventing competitions and simultaneously maintain their health and welfare, appropriate training is mandatory. Specific training and competition management necessitates information about exercise intensities and fitness, which are usually assessed by standardized exercise tests. Our purpose was to identify parameters whose measurement can be largely integrated in the daily training and competition routine and which provide information on fitness and adequacy of applied training. Further, we evaluated training and competition intensities of eventing horses, based on data generated by a proportionally large group of horses over several years. Data of 187 horses from 1-star to 4-star level over a time period of six years were collected during 410 training sessions and 916 Cross Country rides, including measurement of covered distance and speed (GPS, Fidelak EquiPILOT), continuously recording of heart rate (HR) (Polar T52H) and determination of blood lactate concentrations (BLC) (Dr. Lange photometer). Characteristics of the track, altitude profile, ambient temperature and humidity were recorded at each session. The results of the one-way ANOVA indicated a significant effect of competition level on HR (F3,373=23.29; p<0.001) and BLC (F3,763=46.12; p<0.001). Under competition conditions, HR and BLC increased from 1-star to 3-star level (1-star: HR=194±9 bpm, BLC=7.7±5.6 mmol/l; 2-star: HR=198±9 bpm, BLC=9.6±6.2 mmol/l; 3-star: HR=205±10, BLC=15.6±9.8 mmol/l; 4-star: HR=207±2 bpm, BLC=12.4±9.3 mmol/l), while under training conditions, they decreased with increasing competition level (1-star: HR=184±29 bpm; BLC=18.9±11.1 mmol/l; 2-star: HR=182±19 bpm; BLC=12.1±9.0; 3-star: HR=174±25 bpm; BLC=6.9±7.1 mmol/l; 4-star: HR=161±24 bpm, BLC=4.0±5.6 mmol/l). On 1-/2-star level, BLCs after training exceeded those after competition. Reverse applied for 3-/4-star level. On 3-star level, the percentage of HRs above 200 bpm during Cross Country was considerably greater than on lower levels (1-star: 33%; 2-star: 54%; 3-star: 94%). The competition format (CCI/CIC) had no significant effect on the progress of HR. The altitude profile however, had a significant effect on HR (F1,201=26.72, p<0.001) and BLC (F1,89=25.56; p<0.001). Evaluation of physiological response to training and competition through implementation of measurement technology allows an assessment of the different impacts on exercise intensities and should be more commonly used to assess appropriateness of training and competition management in eventing horses. It should be further investigated if the detected discrepancies in the response to training and competition between levels are a result of different fitness ore varying training strategies. LP To meet the demands of international Eventing competitions, a systematic training of horses is necessary. Close monitoring of physiological response to exercise during training and competition can help to improve training. Keywords Exercise physiology; training; eventing; heart rate; lactate. [less ▲]

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See detailClinical effects of computed tomography-guided lumbosacral facet joint, transforaminal epidural, and translaminar epidural injections of methylprednisolone acetate in healthy dogs.
Liotta, Annalisa Pia ULg; Girod, Maud ULg; Peeters, Dominique ULg et al

in American Journal of Veterinary Research (2016), 77(10), 1132-9

OBJECTIVE To determine clinical effects of CT-guided lumbosacral facet joint, transforaminal epidural, and translaminar epidural injections of methylprednisolone acetate in healthy dogs. ANIMALS 15 ... [more ▼]

OBJECTIVE To determine clinical effects of CT-guided lumbosacral facet joint, transforaminal epidural, and translaminar epidural injections of methylprednisolone acetate in healthy dogs. ANIMALS 15 healthy Beagles. PROCEDURES Dogs were randomly assigned to 3 groups (5 dogs/group) and received a single CT-guided lumbosacral facet joint, transforaminal epidural, or translaminar epidural injection of methylprednisolone acetate (0.1 mg/kg). Contrast medium was injected prior to injection of methylprednisolone to verify needle placement. Neurologic examinations were performed 1, 3, 7, and 10 days after the injection. In dogs with neurologic abnormalities, a final neurologic examination was performed 24 days after the procedure. RESULTS Methylprednisolone injections were successfully performed in 14 of the 15 dogs. In 1 dog, vascular puncture occurred, and the methylprednisolone injection was not performed. No major or minor complications were identified during or immediately after the procedure, other than mild transient hyperthermia. During follow-up neurologic examinations, no motor, sensory, or postural deficits were identified, other than mild alterations in the patellar, withdrawal, cranial tibial, and perineal reflexes in some dogs. Overall, altered reflexes were observed in 11 of the 14 dogs, during 27 of 65 neurologic examinations. CONCLUSIONS AND CLINICAL RELEVANCE Results suggested that CT-guided lumbosacral facet joint, transforaminal epidural, and translaminar epidural injections of methylprednisolone acetate were associated with few complications in healthy dogs. However, the number of dogs evaluated was small, and additional studies are needed to assess clinical efficacy and safety of these procedures. [less ▲]

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See detailTECHNIQUE, DIFFICULTY, AND ACCURACY OF COMPUTED TOMOGRAPHY-GUIDED TRANSLAMINAR AND TRANSFORAMINAL LUMBOSACRAL EPIDURAL AND INTRAARTICULAR LUMBAR FACET JOINT INJECTIONS IN DOGS.
Liotta, Annalisa Pia ULg; Sandersen, Charlotte ULg; Couvreur, Thierry et al

in Veterinary radiology & ultrasound : the official journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association (2016), 57(2), 191-8

In human medicine, spinal pain and radiculopathy are commonly managed by computed tomography (CT)-guided facet joint injections and by transforaminal or translaminar epidural injections. In dogs, CT ... [more ▼]

In human medicine, spinal pain and radiculopathy are commonly managed by computed tomography (CT)-guided facet joint injections and by transforaminal or translaminar epidural injections. In dogs, CT-guided lumbosacral epidural or lumbar facet joint injections have not been described. The aim of this experimental, ex vivo, feasibility study was to develop techniques and to assess their difficulty and accuracy. Two canine cadavers were used to establish the techniques and eight cadavers to assess difficulty and accuracy. Contrast medium was injected and a CT scan was performed after each injection. Accuracy was assessed according to epidural or joint space contrast opacification. Difficulty was classified as easy, moderately difficult, or difficult, based on the number of CT scans needed to guide insertion of the needle. A total of six translaminar and five transforaminal epidural and 53 joint injections were performed. Translaminar injections had a high success rate (100%), were highly accurate (75%), and easy to perform (100%). Transforaminal injections had an moderately high success rate (75%), were accurate (75%), and moderately difficult to perform (100%). Success rate of facet joint injections was 62% and was higher for larger facet joints, such as L7-S1. Accuracy of facet joint injections ranged from accurate (37-62%) to highly accurate (25%) depending on the volume injected. In 77% of cases, injections were moderately difficult to perform. Possible complications of epidural and facet joint injections were subarachnoid and vertebral venous plexus puncture and periarticular spread, respectively. Further studies are suggested to evaluate in vivo feasibility and safety of these techniques. [less ▲]

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See detailImpact of feeding and housing on the development ofosteochondrosis in foals—A longitudinal study
Mendoza García, Luis ULg; Lejeune, Jean-Philippe ULg; Caudron, Isabelle ULg et al

in Preventive Veterinary Medicine (2016), 127

Osteochondrosis dissecans (OCD) is a developmental orthopedic disease caused by a failure of the endochondral ossification in epiphyseal plates and joint cartilage. This trouble may induce the presence of ... [more ▼]

Osteochondrosis dissecans (OCD) is a developmental orthopedic disease caused by a failure of the endochondral ossification in epiphyseal plates and joint cartilage. This trouble may induce the presence of osteochondral fragments in the articulation, fissures or subchondral bone cysts in the growth cartilage. Occurrence of osteochondrosis is influenced by a complex interaction of different factors. Among these, the effect of the housing and the feeding of the foals during their first months of life, have been described as risk factors for the development of osteochondrosis. The aim of this study was to investigate the evolution of OCD lesions with a longitudinal study in 204 young foals from 6 to 18 months in comparison to the type of feeding and the type of housing conditions. These factors and OCD status were obtained by a questionnaire and radiological examination, respectively. This allowed dividing the foals into four groups according to the initial OCD status and the evolution of the condition. As a result, we found that foals fed with concentrates show a higher probability to develop OCD lesions (p = 0.06), while foals not receiving concentrates, had a higher probability to heal from existing OCD lesions (p = 0.001). This study supports the theory that management factors such as feeding or housing may influence the evolution of the osteochondrosis disease. [less ▲]

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See detailDevelopment of a field test to evaluate colostrum quality (IgG) in cattle
Guyot, Hugues ULg; Dubreucq, Pierre ULg; Lebreton, Pascal et al

Poster (2015, June)

Failure of transfer of immunity from dam’s colostrum generates a negative effect on calves’ health leading to increased morbidity and mortality (De Nise et al., 1989; Wittum and Perino, 1995 ... [more ▼]

Failure of transfer of immunity from dam’s colostrum generates a negative effect on calves’ health leading to increased morbidity and mortality (De Nise et al., 1989; Wittum and Perino, 1995). Immunoglobulins (IgG) content of colostrum is highly variable and cannot be predicted. Distinguishing good from poor quality colostrum allows to adapt the volume administered or to initiate ancillary procedures for a sufficient transfer of IgG. The aim of the study was to evaluate the performances of a field test for colostrum quality determination. [less ▲]

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See detailDevelopment of a field test to evaluate colostral immunity transfer in young calves
Guyot, Hugues ULg; Dubreucq, Pierre ULg; Lebreton, Pascal et al

Poster (2015, June)

Failure of transfer of immunity from dam’s colostrum generates a negative effect on calves’ health leading to increased morbidity and mortality (De Nise et al., 1989; Wittum and Perino, 1995). Unawareness ... [more ▼]

Failure of transfer of immunity from dam’s colostrum generates a negative effect on calves’ health leading to increased morbidity and mortality (De Nise et al., 1989; Wittum and Perino, 1995). Unawareness of the colostral quality and variation in the calf’s capability to efficiently absorb immunoglobulins (IgG) supports the need for specific evaluation of the immunity transfer at a herd level. The aim of the study was to evaluate the performances of a field test for passive immunity transfer (PIT) in calves. [less ▲]

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See detailAge-dependent expression of osteochondrosis-related genes in equine leukocytes
Mendoza García, Luis ULg; Piquemal, David; Lejeune, Jean-Philippe ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2015), 2

Introduction: Osteochondrosis (OC) is a developmental disease in horses which has a significant impact on the horse’s welfare and performance. The early disturbance in the process of endochondral ... [more ▼]

Introduction: Osteochondrosis (OC) is a developmental disease in horses which has a significant impact on the horse’s welfare and performance. The early disturbance in the process of endochondral ossification progresses to inflammatory and repair processes in older horses. Previously, differentially expressed genes in leukocytes of OC-affected horses have been identified. The aim of the present study is to detect age-related changes in these differentially expressed genes. Materials and Methods: The expression of OC-related genes was analysed by real-time PCR and subsequent statistical analysis (ΔΔCT) in the leukocytes of 135 Belgian Warmblood horses divided into three different age groups: 30 months (n=38). Results: Relative expression of genes of horses less than 12 months of age showed significant induction of the genes MGAT4A, PRKCG, MHCI, ApoB, ApoB3G, B4GALT6 and a significantly lower expression of the genes OAS3. Horses of 18–24 months of age, showed a significantly higher expression of the genes TBC1D9, MGAT4A, IFIH1, MHCIIa and MMP1. Horses of more than 30 months of age showed a significantly higher expression of the genes MGAT4A, HP, SECTM1 compared with their age-matched control groups. Conclusions: The study demonstrates that OC-related genes are differentially expressed in horses of different ages compared with their age-matched controls. Some of the genes may be implicated in cell signalling and differentiation as well as carbohydrate and lipid metabolism and inflammation. However, the causal relationship between the differentially expressed genes and the development and progression of the OC lesions needs to be determined. [less ▲]

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See detailFeasibility of ultrasound-guided epidural access at the lumbo-sacral space in dogs
Liotta, Annalisa Pia ULg; Busoni, Valeria ULg; Carrozzo et al

in Veterinary Radiology & Ultrasound (2015), 56(2), 220-228

Epidural injections are commonly performed blindly in veterinary medicine. The aims of this study were to describe the lumbosacral ultrasonographic anatomy and to assess the feasibility of an ultrasound ... [more ▼]

Epidural injections are commonly performed blindly in veterinary medicine. The aims of this study were to describe the lumbosacral ultrasonographic anatomy and to assess the feasibility of an ultrasound-guided epidural injection technique in dogs. A cross sectional anatomic atlas of the lumbosacral region and ex vivo ultrasound images were obtained in two cadavers to describe the ultrasound anatomy and to identify the landmarks. Sixteen normal weight canine cadavers were used to establish two variations of the technique for direct ultrasound-guided injection, using spinal needles or epidural catheters. The technique was finally performed in two normal weight cadavers, in two overweight cadavers and in five live dogs with radiographic abnormalities resulting of the lumbosacral spine. Contrast medium was injected and CT was used to assess the success of the injection. The anatomic landmarks to carry out the procedure were the seventh lumbar vertebra, the iliac wings, and the first sacral vertebra. The target for directing the needle was the trapezoid-shaped echogenic zone between the contiguous articular facets of the lumbosacral vertebral canal visualized in a parasagittal plane. The spinal needle or epidural catheter was inserted in a 45° craniodorsal-caudoventral direction through the subcutaneous tissue and the interarcuate ligament until reaching the epidural space. CT examination confirmed the presence of contrast medium in the epidural space in 25/25 dogs, although a variable contamination of the subarachnoid space was also noted. Findings indicated that this ultrasound-guided epidural injection technique is feasible for normal weight and overweight dogs, with and without radiographic abnormalities of the spine. [less ▲]

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See detailHeart rate variability as a measure of comfort in the anaesthetised horse
Lacroix, Alice; Gougnard, Alexandra ULg; Cerri, Simona ULg et al

Poster (2015)

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See detailEffects of isoflurane and sevoflurane on the neutrophil myeloperoxidase system of horses
MINGUET, Grégory ULg; Franck, Thierry ULg; JORIS, Jean ULg et al

in Veterinary Immunology and Immunopathology (2015)

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