References of "Saegerman, Claude"
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See detailInfectivity of a recombinant murine norovirus (RecMNV) in Balb/cByJ mice
Mathijs, Elisabeth; de Oliveira-Filho, Edmilson; Dal Pozzo, Fabiana ULg et al

in Veterinary Microbiology (in press)

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See detailThe Added-value of Using Participatory Approaches to Assess the Acceptability of Surveillance Systems: The Case of Bovine Tuberculosis in Belgium
Calba, C; Goutard, FL; Vanholme, L et al

in PLoS ONE (in press)

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See detailResurgence of Schmallenberg virus in Belgium after 3 years of epidemiological silence
Delooz, L; Saegerman, Claude ULg; Quinet, C et al

in Transboundary and Emerging Diseases (in press)

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See detailMicrobiological zoonotic emerging risks, transmitted between livestock animals and humans (2007-2015)
Filippitzi, ME; Goumperis, T; Robinson, T et al

in Transboundary and emerging diseases (in press)

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See detailImproving laboratory diagnostic efficiency of epizootic diseases in Belgium
Cargnel, Mickaël ULg; Roelandt, Sophie; Van der Stede, Yves et al

Poster (2016, March)

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See detailIdentification of specific bovine blood biomarkers with a non-targeted approach using HPLC ESI tandem mass spectrometry
Lecrenier, Marie-Caroline ULg; Marbaix, Hélène; Dieu, Marc et al

in Food Chemistry (2016), 213

Animal by-products are valuable protein sources in animal nutrition. Among them are blood products and blood meal, which are used as high-quality material for their beneficial effects on growth and health ... [more ▼]

Animal by-products are valuable protein sources in animal nutrition. Among them are blood products and blood meal, which are used as high-quality material for their beneficial effects on growth and health. Within the framework of the feed ban relaxation, the development of complementary methods in order to refine the identification of processed animal proteins remains challenging. The aim of this study was to identify specific biomarkers that would allow the detection of bovine blood products and processed animal proteins using tandem mass spectrometry. Seventeen biomarkers were identified: nine peptides for bovine plasma powder; seven peptides for bovine haemoglobin powder, including six peptides for bovine blood meal; and one peptide for porcine blood. They were not detected in several commercial compound feed or feed materials, such as blood by-products of other animal origins, milk-derived products and fish meal. These biomarkers could be used for developing a species-specific and blood-specific detection method. [less ▲]

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See detailGenome Sequences of Four Strains of Mycobacterium avium subsp. hominissuis, Isolated from Swine and Humans, Differing in Virulence in a Murine Intranasal Infection Model
Bruffaerts; Vluggen, C; Duytschaever, L et al

in Genome Announcements (2016), 4(3),

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See detailRisk factors and effect of selective removal on retroviral infections prevalence in Belgian stray cats.
Garigliany, Mutien-Marie ULg; Jolly, Sandra ULg; Dive, M. et al

in The Veterinary record (2016), 178(2), 45

The aim of this study was to evaluate the effect of several risk/protective factors and predictors on the prevalence of feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) infections in ... [more ▼]

The aim of this study was to evaluate the effect of several risk/protective factors and predictors on the prevalence of feline immunodeficiency virus (FIV) and feline leukaemia virus (FeLV) infections in 302 stray cats captured during a trap-neuter-release programme in a mixed urban-rural area from Belgium, from 2010 to 2012. The impact of selective removal of FIV-positive cats on the apparent prevalence in the remaining population over this three-year period was also assessed. The seroprevalences over three years were 18.8 per cent for FIV and 0.7 per cent for FeLV. For FIV, the seroprevalence decreased significantly from the first year of the programme (2010; 30.5 per cent) to the last (2012; 13.1 per cent). Sex (male) and age (adult and old cats) were risk factors, while the year of sampling (years 2011 and 2012) was a protective factor. Age, sex and location were the most relevant predictors of FIV status. The data presented in this study revealed a very high FIV seroprevalence in Belgian stray cats, while FeLV was almost absent. The selective removal of positive cats had a drastic effect on the FIV seroprevalence in the remaining cat population. [less ▲]

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See detailStakeholders’ perceptions, attitudes and practices towards risk prevention in the food chain
Lupo, C; Wilmart, O; Van Huffel, X et al

in Food Control (2016), 66

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See detailClinical sentinel surveillance of equine West Nile fever, Spain
Saegerman, Claude ULg; Alba-Casals, A; Garcia-Bocanegra, I et al

in Transboundary and Emerging Diseases (2016), 63(2), 184-193

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See detailGenotyping and strain distribution of Mycobacterium avium subspecies hominissuis isolated from humans and pigs in Belgium, 2011-2013
Vluggen, C; Soetaert, K; Duytschaever, L et al

in Eurosurveillance (2016), 21(3), 18-25

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See detailReconstruction of the Schmallenberg virus epidemic in Belgium: Complementary use of disease surveillance approaches
Poskin, A; Theron, Léonard ULg; Hanon, JB et al

in Veterinary Microbiology (2016), 183

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See detailEpidemiology and Diagnosis of Q Fever in Animals and Humans in the 21st Century
Mainil, Jacques ULg; Monseur, Christine ULg; Saegerman, Claude ULg et al

Scientific conference (2015, November 13)

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See detailBovine blood biomarkers as a way of processed animal proteins detection in feedingstuffs
Lecrenier, Marie-Caroline ULg; Marbaix, Hélène; Veys, Pascal et al

Poster (2015, November)

The prohibition of using animal by-products in feedingstuffs depends on two factors: their nature defined by the tissue/cell type and the species of origin, and on their destination (pets, fur animals or ... [more ▼]

The prohibition of using animal by-products in feedingstuffs depends on two factors: their nature defined by the tissue/cell type and the species of origin, and on their destination (pets, fur animals or other farmed animals). Proteomics is particularly well-suited to the purpose of PAPs detection as it is a tissue and species-specific method. The aim of this study was the identification and the selection of specific peptide biomarkers using tandem mass spectrometry for the detection of bovine blood products and blood meals in animal feed. Twenty-nine samples of blood meals and blood products (plasma or haemoglobin powder) of porcine, poultry and bovine origin as well as three milk products and two fish meals were analysed using a Q TOF mass spectrometer. Vegetal feed samples adulterated with 1% or 10% of bovine plasma powder, haemoglobin powder or blood meal were also analysed to evaluate the applicability of the method. Four proteins of interest were highlighted: Alpha-2-macroglobulin, apolipoprotein A-1, serotransferrin and haemoglobin (α and β chains). From these proteins, sixteen peptides were identified as potential bovine blood biomarkers in feedingstuffs. Nine of them could be used for the detection of plasma powder and seven of them for haemoglobin powder or blood meal. The evaluation of these peptides by a search against NCBInr database revealed that some of them could also be used to detect other ruminant bloods such as ovine or caprine ones. These preliminary results are promising. Efforts are now focused to improve the protocol in order to increase the sensitivity of the method as regards the selected proteins. [less ▲]

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See detailBelgian Wildlife as Potential Zoonotic Reservoir of Hepatitis E virus
Thiry, Damien ULg; Mauroy, Axel ULg; Saegerman, Claude ULg et al

in Transboundary and Emerging Diseases (2015)

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See detailImproving laboratory diagnostic capacity of epizootic diseases in Belgium
Cargnel, Mickaël ULg; Roelandt, Sophie; Van der Stede, Yves et al

Poster (2015, October 30)

Belgium and other European neighbouring countries faced several emerging and re-emerging diseases as well as zoonotic diseases over the last decade. However, it has been noticed that during these episodes ... [more ▼]

Belgium and other European neighbouring countries faced several emerging and re-emerging diseases as well as zoonotic diseases over the last decade. However, it has been noticed that during these episodes, the laboratory diagnostic capabilities were surpassed, which led to an increase in the time required for the control and the eradication of these diseases. Belgium is a European hub and can be affected by these diseases which can via different paths e.g. trade of live animals or animal products from around the world or via wildlife. It is therefore crucial to react rapidly to these diseases, to establish contingency plans and to develop appropriate diagnostic tests. Moreover, there are only few publications looking at the issues of increased diagnostic capacities for epizootic diseases based on [less ▲]

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See detailApplying participatory approaches in the evaluation of surveillance systems: A pilot study on African swine fever surveillance in Corsica.
Calba, Clémentine; Antoine-Moussiaux, Nicolas ULg; Charrier, François et al

in Preventive Veterinary Medicine (2015)

The implementation of regular and relevant evaluations of surveillance systems is critical in improving their effectiveness and their relevance whilst limiting their cost. The complex nature of these ... [more ▼]

The implementation of regular and relevant evaluations of surveillance systems is critical in improving their effectiveness and their relevance whilst limiting their cost. The complex nature of these systems and the variable contexts in which they are implemented call for the development of flexible evaluation tools. Within this scope, participatory tools have been developed and implemented for the African swine fever (ASF) surveillance system in Corsica (France). The objectives of this pilot study were, firstly, to assess the applicability of participatory approaches within a developed environment involving various stakeholders and, secondly, to define and test methods developed to assess evaluation attributes. Two evaluation attributes were targeted: the acceptability of the surveillance system and its the non-monetary benefits. Individual semi-structured interviews and focus groups were implemented with representatives from every level of the system. Diagramming and scoring tools were used to assess the different elements that compose the definition of acceptability. A contingent valuation method, associated with proportional piling, was used to assess the non-monetary benefits, i.e., the value of sanitary information. Sixteen stakeholders were involved in the process, through 3 focus groups and 8 individual semi-structured interviews. Stakeholders were selected according to their role in the system and to their availability. Results highlighted a moderate acceptability of the system for farmers and hunters and a high acceptability for other representatives (e.g., private veterinarians, local laboratories). Out of the 5 farmers involved in assessing the non-monetary benefits, 3 were interested in sanitary information on ASF. The data collected via participatory approaches enable relevant recommendations to be made, based on the Corsican context, to improve the current surveillance system. [less ▲]

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See detailCurrent status of fasciolosis in Vietnam: an update and perspectives
Bui Thi, Dung ULg; Pham, Ngoc Doanh; Saegerman, Claude ULg et al

in Journal of Helminthology (2015)

Vietnam is recognized as endemic for fasciolosis. However, most of the available publications have not been published in international journals. This review is based on national and international ... [more ▼]

Vietnam is recognized as endemic for fasciolosis. However, most of the available publications have not been published in international journals. This review is based on national and international Vietnamese publications and highlights the current status of fasciolosis in Vietnam. It also provides some information available in neighboring countries. Updated data on responsible species, distribution, transmission and control aspects, are summarized. The Central region of Vietnam is reported as being highly endemic for fasciolosis with a high number of human patients (more than 20.000 in 2011). Fasciola gigantica is reported as the main species in Vietnam. However, hybrids between F. gigantica and F. hepatica were identified. Both human and animals are infected by the ingestion of raw vegetables and possibly contaminated drinking water. Three lymnaeid snail species (Austropeplea viridis, Radix auricularia, Radix rubiginosa) may act as intermediate host of Fasciola spp. However due to the likely misidentification of snail species and cercariae during the previous decade the critical analysis of published data is difficult. A better understanding of transmission aspects of fasciolosis would allow the implementation of preventive measures of this important neglected zoonotic disease. [less ▲]

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