References of "Sacheli, Rosalie"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailThe miR-183/ItgA3 axis is a key regulator of prosensory area during early inner ear development
Van Den Ackerveken, Priscilla ULiege; Mounier, Anaïs ULiege; Huyghe, Aurélia et al

in Cell Death & Differentiation (in press)

Detailed reference viewed: 59 (15 ULiège)
Full Text
Peer Reviewed
See detailEvaluation of the Xpert®GBS LB test (Cepheid) performed on antenatal screening LIM enrichment broth for detection of Streptococcus agalactiae or group B streptococcus, compared to the reference culture method.
MEEX, Cécile ULiege; DUPONT, Audrey; SACHELI, Rosalie ULiege et al

Conference (2017, October 19)

PCR, performed on LIM enrichment broth compared wit the reference method, that is subculture on selective differential agar performed from the same incubated Lim broth inoculated with a vagino-rectal swab ... [more ▼]

PCR, performed on LIM enrichment broth compared wit the reference method, that is subculture on selective differential agar performed from the same incubated Lim broth inoculated with a vagino-rectal swab collected at 35-37 weeks’ gestation. Material/methods: During an 8-months period in 2015-2016, series of consecutive vagino/rectal swabs collected for antenatal GBS screening (at the university hospital of Liege, Belgium) were plated first on Granada agar and then inoculated in selective enrichment LIM broth. The incubated broth was further sub-cultured on Granada and Biorad StrepBselect agars. Moreover, a sterile swab immersed in the same incubated broth was further analyzed by a real-time PCR targeting GBS using the Xpert®GBS LB test on the GeneXpert® system (Cepheid). Results: Among the 288 antenatal screenings included in the study, 48 (16.7%) were positive for GBS using the culture reference method and 51 Xpert®GBS LB test were positive (17.7%), includin the 48 samples positive in culture and 3 additional specimens for witch culture remained negative. Considering the enriched culture as the gold standard, the sensitivity and specificity of the Xpert®GBS LB test were 100% and 98.8% respectively. Conclusions: The Xpert®GBS LB test performed on incubated LIM broth is at least as efficient as selective enriched culture for antenatal screening of GBS. The turnaround-time and hands-on-ti are much shorter for the Xpert® GBS LB but it is more expensive than culture method, which may limit its use. [less ▲]

Detailed reference viewed: 33 (3 ULiège)
Full Text
Peer Reviewed
See detailDisseminated histoplasmosis: case report and review of the literature
Evrard, Séverine; CAPRASSE, Philippe ULiege; Gavage, Pierre et al

in Acta Clinica Belgica (2017)

Case report: We report the case of a young Cameroonian woman who presented with cough, hyperthermia, weight loss, pancytopenia, and hepatosplenomegaly. A positive HIV serology was discovered and a chest ... [more ▼]

Case report: We report the case of a young Cameroonian woman who presented with cough, hyperthermia, weight loss, pancytopenia, and hepatosplenomegaly. A positive HIV serology was discovered and a chest radiography revealed a ‘miliary pattern’. Bone marrow aspiration pointed out yeast inclusions within macrophages. Given the morphological aspect, the clinical presentation and immunosuppression, histoplasmosis was retained as a working hypothesis. Antiretroviral and amphotericin B treatments were promptly initiated. Review: Given the immigration wave that Europe is currently experiencing, we think it is important to share experience and knowledge, especially in non-endemic areas such as Europe, where clinicians are not used to face this disease. Histoplasmosis is due to Histoplasma capsulatum var. capsulatum, a dimorphic fungus. Infection occurs by inhaling spores contained in soils contaminated by bat or bird droppings. The clinical presentation depends on the immune status of the host and the importance of inoculum, varying from asymptomatic to disseminated forms. AIDS patients are particularly susceptible to develop a severe disease. Antigen detection, molecular biology techniques, and microscopic examination are used to make a rapid diagnosis. However, antigen detection is not available in Europe and diagnosis needs a strong clinical suspicion in non-endemic areas. Because of suggestive imagery, clinicians might focus on tuberculosis. Our case illustrates the need for clinicians to take histoplasmosis [less ▲]

Detailed reference viewed: 17 (2 ULiège)
Full Text
Peer Reviewed
See detailEvolution of rate and genotypes of resistance to macrolide/lincosamide among invasive Group B Streptococcus (GBS): Development of a multiplex PCR tool for simultaneous detection of ErmB, ErmTr, MefA and LsaC resistance genes.
SACHELI, Rosalie ULiege; DESCY, Julie ULiege; MEEX, Cécile ULiege et al

Poster (2017, October)

Methods: A multiplex-PCR, using a set of specifically designed or already described (Kataja, 1999; Malbruny, 2011) primers was developed and used to detect, in GBS, three genes for erythromyc resistance ... [more ▼]

Methods: A multiplex-PCR, using a set of specifically designed or already described (Kataja, 1999; Malbruny, 2011) primers was developed and used to detect, in GBS, three genes for erythromyc resistance, ermB, ermTR, mefA and one gene for clindamycin-resistance lsaC. AdhP gene amplification was used as control for GBS identification. All(219) GBS isolates from invasive infections in newborns and adults received by the Belgian National Reference Center for GBS in 2015, and control strains were tested for erythromycin/clindamycin susceptibility (disk-diffusion/broth- microdilution) and for detection of resistance genes. Results: PCR products demonstrated the expected respective sizes. The method has been validated successfully according to ISO15189 analytical requirements. Of the 219 isolates, 67(30,67%) w resistant to erythromycin and/or clindamycin: 44/67(65,78%) showed a constitutive-MLS phenotype and 10/67(14,9%) the inducible-MLS phenotype. Among the constitutive-MLS strains, 73% harboured ErmB gene, 13% ErmTR, 7% ErmB+mefA and 7% ermB together with LsaC gene. The inducible-MLS strains harboured mostly ErmTr gene (89%) and the others the ErmB gene. Among the 10/67(14,9%) GBS strains with an M-phenotype (isolated resistance to erythromycin), the MefA gene was exclusively detected. Among the 3(4,48%) strains showing an isolated resistance to clindamycin (L-phenotype), the LsaC gene was detected. Conclusion: The developed multiplex PCR is able to detect simultaneously four genes involved in MLS resistance in GBS. In 2015, 30,6% of the invasive GBS strains isolated in Belgium were resist to macrolides and/or lincosamides. The emergence of the L-phenotype in GBS described since 2010, justifies the relevance to also detect LsaC gene together with ErmB, ErmTr and MefA. [less ▲]

Detailed reference viewed: 29 (3 ULiège)
Full Text
Peer Reviewed
See detailPrevalence and capsular-polysaccharide type distribution of colonizing group B streptococci (GBS) isolated from recto-vaginal samples in pregnant women in Hanoï, Vietnam
MEEX, Cécile ULiege; DEVEY, Anaïs ULiege; PHAM HONG, Nhung et al

Poster (2017, October)

Background: The study was organized by the Belgian National Reference Center (NRC) for Streptococcus agalactiae or GBS, and carried out in Vietnam. The aim of the study was to determine the prevalence of ... [more ▼]

Background: The study was organized by the Belgian National Reference Center (NRC) for Streptococcus agalactiae or GBS, and carried out in Vietnam. The aim of the study was to determine the prevalence of GBS colonization among pregnant women in Hanoï and to characterize the capsular-polysaccharide (CPS) type of the isolated strains. Methods: For a 2-months period in 2015, 888 recto-vaginal swabs were collected in Bach-Mai-Hospital from pregnant women at 35-37 weeks’ gestation and were cultured for detection of GBS. Strains were stored and transferred to the Belgian NRC for further characterization. CPS-typing was performed by both latex agglutination and PCR (Poyart, 2007; Kong, 2008). Results: Among the 888 swabs, 111 were positive for GBS, that is a prevalence of colonization of 12.5%. A total of 90 strains were available for typing: 91,11% could be serotyped by latex agglutination and all the strains, including the 8 phenotypically non-typable strains, were successfully genotyped. CPS type V was the most prevalent (36.7%) followed by CPS types Ib (25.6%), III (21.1%), VI and VII (8.9% and 4.4%). CPS type II was found twice and serotype Ia was found once. CPS types IV, VIII and IX weren’t present in this population. Conclusion: With predominance of types V, Ib and III, this distribution of CPS-types of GBS colonizing pregnant women in Hanoï, Vietnam, differs from distributions described in Europe and in o Asian countries. This study provides useful information for the development of a universal vaccine that could contribute to improve the prevention of neonatal GBS infections. [less ▲]

Detailed reference viewed: 29 (3 ULiège)
Full Text
Peer Reviewed
See detailUpdate of the characteristics of Group B Streptococci (GBS) colonizing pregnant women in Belgium: capsular-type distribution, pili characterization, antimicrobial susceptibility profile and Multiple Locus Sequence Types.
SACHELI, Rosalie ULiege; MEEX, Cécile ULiege; DESCY, Julie ULiege et al

Poster (2017, October)

Aim: Improving knowledge and characterization of GBS strains colonizing pregnant women in Belgium. Methods: In 2013, collection of 387 strains of GBS from 80 laboratories participating in a national ... [more ▼]

Aim: Improving knowledge and characterization of GBS strains colonizing pregnant women in Belgium. Methods: In 2013, collection of 387 strains of GBS from 80 laboratories participating in a national survey among pregnant woman. For each strain, determination of capsular-polysaccharide type agglutination and PCR, of pili-type by PCR and of antimicrobial susceptibility by disk-diffusion, broth-microdilution and detection of resistant genes by PCR. For serotype III strains, determination sequence-type by Multiple-Locus Sequence-Typing (MLST). Results: Serotype III was the most prevalent (28.5%) followed by serotypes V, Ia, II, IV and Ib (20.4%, 19.9%, 17.8%, 7%, 5.4%). Serotypes VI, VII and IX were found each once. All strains remained susceptible to penicillin (MICs: 0.03-0.125 mg/L) and other beta-lactams tested; 28.7% were resistant to erythromycin and 26.7% to clindamycin. With regards to pili, all 387 strains harboured one the PI-2 variants alone or in combination and 70.3% contained PI-1. The 110 serotype III isolates were resolved into 18 STs. The most common were ST-17 (35.5%) followed by ST-19 (30%) and ST- ST-27, ST-23 (<=5%). Conclusion: Among GBS from colonized pregnant women in Belgium: capsular-type and pili distributions, and MLST profile among type III strains were quite similar to reported data from Europ and USA during the last decade. As showed in this study, penicillin remains the first line drug of choice. On the contrary, resistance rates against macrolides/lincosamide, has reached a plateau since a decade, but it is noteworthy to notify the emergence of strains with isolated resistance to clindamycine. [less ▲]

Detailed reference viewed: 27 (4 ULiège)
Full Text
See detailVibrio spp chez l’Homme - Vibrio spp bij de mens
MELIN, Pierrette ULiege; SACHELI, Rosalie ULiege

in POLET, Marie (Ed.) Vibrio spp dans l’alimentation et chez l’Homme - Formation (2016, December 15)

Detailed reference viewed: 25 (6 ULiège)
Full Text
Peer Reviewed
See detailApplication of rep-PCR as molecular typing method for dermatophytes
SACHELI, Rosalie ULiege; ADJETEY BAHUN, Akolé ULiege; DARFOUF, Rajae ULiege et al

Conference (2016, October 29)

Background The distinction of dermatophytes strains by conventional methods is problematic due to a lack of reliable characteristics between isolates. The ability to identify individual organisms allows ... [more ▼]

Background The distinction of dermatophytes strains by conventional methods is problematic due to a lack of reliable characteristics between isolates. The ability to identify individual organisms allows identification of infection sources, such as communal swimming and public washing rooms and apply appropriate control measures. Several outbreaks of dermatophytes infection have already been described in Europe and the feasibility of comparing DNA fingerprints is of interest in this particular field. We have successfully applied the rep-PCR method for typing dermatophytes species such as Microsporum audouinii, Trichophyton violaceum and Trichophyton mentagrophytes complex (T.mentagrophytes complex). Methods The Diversilab® system (bioMérieux, France) has been used to perform rep-PCR. Strains of M. audouinii (116) and T. violaceum (23) were collected during a national survey conducted by the National Reference center (NRC) for mycoses (Liège) in Belgium from February 2013 to March 2014. Strains of T. mentagrophytes complex (32) are part of the national collection of the NRC collected between 2012 and 2015. Results The Diversilab® is able to distinguish different subspecies between the same species complex as it was confirmed with the T. mentagrophytes complex. For this group of strains, two major groups are clearly visible after Diversilab analysis and correspond to white strains (ITS sequenced as T. interdigitale) and yellow strains (ITS sequenced as A. benhamiae) of T. mentagrophytes complex. Both groups share only 70% of similarity. Within a same species of dermatophyte, the Rep-PCR is also able to define several subgroups sometimes linked with epidemiological factors. Inside the T. violaceum strains circulating in Belgium, two different DNA fingerprints have been found. These two subgroups were well separated regarding the geographical location in Belgium (Wallonia and Flanders). Inside the M. audouinii species, 6 different DNA fingerprints have been detected with a main group. The degree of similarity between these six groups is comprised between 85,6% and 94%. Strains belonging to the six groups were defined as M. audouinii by ITS sequencing. Conclusion The DiversiLab® system with the Rep-PCR technology proved to be an efficient method for dermatophytes molecular typing. It is highly sensitive to discriminate small genotypic variation between two strains within the same species which is not possible with other molecular methods such as ITS sequencing. Inside the T. mentagrophytes complex, it permits to easily distinguish the yellow zoophilic strains from the white anthropophilic strain, hardly possible by microscopy. [less ▲]

Detailed reference viewed: 34 (13 ULiège)
Full Text
Peer Reviewed
See detailUnusual Species of Dermatophytes: Rarely Identified or New?
HAYETTE, Marie-Pierre ULiege; SACHELI, Rosalie ULiege

in Mycopathologia (2016)

Dermatophytes are causing superficial mycosis in animals and humans. Depending on the geophilic, zoophilic or anthropophilic origin of the fungus but also on the immunological status of the patient ... [more ▼]

Dermatophytes are causing superficial mycosis in animals and humans. Depending on the geophilic, zoophilic or anthropophilic origin of the fungus but also on the immunological status of the patient, symptomatology can widely differ. Nevertheless, each species is currently associated with typical clinical manifestations, even if atypical localizations and/or clinical pictures are sometimes also reported. Diagnostic tools applied to species identification have been changing since the last two decades with the more frequent use of molecular methods currently considered nowadays as reference methods for species identification. It becomes obvious that the algorithm used for the distinction of closely related species needs to combine phenotypic and genomic methods. All these different points are discussed, and the most recent novel species causing or involved in human dermatophytosis are reported. [less ▲]

Detailed reference viewed: 21 (6 ULiège)
Full Text
Peer Reviewed
See detailGenotypic characterization of T. mentagrophytes complex strains circulating in Belgium with the Diversilab® system.
SACHELI, Rosalie ULiege; Utri, Tania ULiege; ADJETEY BAHUN, Akolé ULiege et al

Poster (2016, April 09)

Background Infections due to the zoophilic dermatophyte Arthroderma benhamiae are being more frequently diagnosed in Belgium since a few years. The most common source of infection is guinea pigs. This ... [more ▼]

Background Infections due to the zoophilic dermatophyte Arthroderma benhamiae are being more frequently diagnosed in Belgium since a few years. The most common source of infection is guinea pigs. This species which is referred to the Trichophyton species of A.benhamiae can cause tinea capitis, tinea corporis, tinea manus and more frequently tinea faciei. These strains appear with a bright yellow thallus in culture and do not easily sporulate making them difficult to identify. Sequencing of these strains reveals “Arthroderma benhamiae”. At the contrary white strains of T. mentagrophytes complex are identified by sequencing as “T. mentagrophytes variety interdigitale” or T. interdigitale. The aim of the study is the evaluation of the genetic heterogeneity of these two subtypes of the T. mentagrophytes complex by using the DiversiLab® system. Material and methods 32 strains were collected by the National Reference Center for mycoses between 2012 and 2015. The fungal strains were identified by microscopy or ITS sequencing. All strains appearing white with a yellow pigment in culture and being identified as “A. benhamiae” by DNA sequencing were included as well reference strains. Some selected strains appearing white in culture and being identified as “T. mentagrophytes var. interdigitale” by DNA sequencing were also included. The genotypic analysis was performed by the DiversiLab® system (bioMérieux) by DNA fingerprinting generation. Results Both groups showed huge differences in DNA fingerprints. The similarity calculated by the DiversiLab® tool between the two groups was 70%. This reflects a high genotypic heterogeneity regarding the two types of strains analyzed. This is surprising given that both groups belong to the same species complex. The comparison of these two distinct DNA fingerprints with the mold database of bioMérieux generated identification as “T. mentagrophytes” for both groups. We noticed that the library contained two distinct patterns of DNA fingerprints (profile 1, strains MK55-60 and profile 2, strains MK138-143). Yellow strains were highly similar to profile 1 and white strains were highly similar to profile 2. Unfortunately, no additional information regarding the subspecies implicated could be obtained from the manufacturer. However, this study demonstrates that it may represent respectively “yellow strains types Trichophyton species of A. benhamiae” and “ white strains types T. interdigitale”. Conclusion This study highlights the genotypic differences between two types of strains belonging to the T. mentagrophytes species complex. Yellow strains which incidence is increasing in Belgium are clearly different from other strains previously characterized and the DiversiLab® method shows a high efficiency for discriminating between these two species difficult to separate by microscopy. [less ▲]

Detailed reference viewed: 35 (6 ULiège)
Full Text
Peer Reviewed
See detailEtude de dermatophytes isolés dans les services de revalidation et physiothérapie d’une structure hospitalière en région Liégeoise (Belgique)
SACHELI, Rosalie ULiege; Utri, Tania ULiege; Abdel-Sadick, Hidjab Abdoulaye ULiege et al

Poster (2016, March 23)

Objectifs : Les dermatophytes sont responsables d’infections de la peau, des ongles et du cuir chevelu. Les espèces anthropophiles peuvent se transmettre d’une personne à une autre par un simple contact ... [more ▼]

Objectifs : Les dermatophytes sont responsables d’infections de la peau, des ongles et du cuir chevelu. Les espèces anthropophiles peuvent se transmettre d’une personne à une autre par un simple contact. Leur présence en milieu sportif (salles de sport, piscine, tatamis), douches collectives, vestiaires des écoles, a été largement décrite. Par contre il existe peu de littérature concernant les établissements de soins. C’est pourquoi, nous nous sommes intéressés au risque potentiel d’infection par des dermatophytes en milieu hospitalier et particulièrement dans les salles de kinésithérapie et de revalidation ainsi que dans une piscine du CHU de Liège. Matériel et méthodes : Trois sites du CHU de Liège ont été investigués: le site du Sart Tilman (ST), le site Ourthe Amblève (OA) et le site Notre-Dame des Bruyères (NDB). Les prélèvements ont été effectués en salle de revalidation et kinésithérapie ainsi qu’à la piscine de revalidation. Ces prélèvements ont été réalisés sur les surfaces à l’aide de géloses de contact (milieu de Sabouraud/chloramphénicol/actidione/gentamicine, Tritium Microbiologie, Pays-bas). L’identification des espèces fongiques a été réalisée par observation macro-microscopique des cultures, spectrométrie de masse (Maldi-Tof) et séquençage moléculaire. Résultats : Au total, 552 prélèvements ont été réalisés parmi lesquels 15 dermatophytes ont été recensés (2,72%). On retrouve: 7 T. rubrum, 7 T. interdigitale, 1 T. mentagrophytes var. mentagrophytes. Sur le site ST, 207 prélèvements ont été effectués parmi lesquels 4 T. rubrum, 1 T. interdigitale et 1 T. mentagrophytes var. mentagrophytes (zoophile). Sur le site OA, parmi les 226 prélèvements effectués, 4 Trichophyton interdigitale et 3 Trichophyton rubrum ont été retrouvés. Aucun dermatophyte n’a été isolé de l’eau de piscine ou pédiluve. Sur le site de NDB, sur 119 prélèvements, seuls 2 isolats de Trichophyton interdigitale ont été identifiés. Les prélèvements positifs pour les dermatophytes concernent des tapis de gymnastique, vélos, rameurs, balances, trampolines, demi-sphères d’équilibre, table de kinésithérapie, sols de douches et vestiaires de la piscine. Conclusion Grâce à ces investigations, nous avons pu mettre en évidence sur le matériel de revalidation et les sols des vestiaires, la présence de deux espèces anthropophiles, T. rubrum et T. interdigitale fréquemment impliquées dans les cas d’onychomycoses et atteintes cutanées. Une souche zoophile de T. mentagrophytes a également été mise en évidence. Cette étude démontre qu’un renforcement de la fréquence de nettoyage des sols et des instruments utilisés dans les salles de sport du CHU de Liège est nécessaire de façon à minimaliser les risques de contamination de patients et du personnel médical. [less ▲]

Detailed reference viewed: 70 (4 ULiège)
Full Text
Peer Reviewed
See detailA One-year Survey of Microsporum audouinii infections in Belgium: epidemiological and genotypic characterisation
SACHELI, Rosalie ULiege; ADJETEY BAHUN, Akolé ULiege; DARFOUF, Rajae ULiege et al

in Clinical Microbiology and Infection (2016), 22(3), 2859-28517

During recent years the proportion of tinea capitis (TC) infections due to Microsporum audouinii (M. audouinii) has increased both in Belgium and other European countries. To better understand the ... [more ▼]

During recent years the proportion of tinea capitis (TC) infections due to Microsporum audouinii (M. audouinii) has increased both in Belgium and other European countries. To better understand the emergence of this species in the country, the Belgian National Reference Center (NRC) for dermatophytes launched an epidemiological survey on the main anthropophilic dermatophytes causing TC in Belgium and including the genomic characterization of M. audouinii isolates. In total, 116 strains of M. audouinii were confirmed and characterized by the DiversiLab® system (bioMérieux). Six genotypic variants were identified, among which one major group included 90 isolates and the reference strain. Another variant group (11 strains) was exclusively confined to a geographical region in South Belgium. Analysis of epidemiological characteristics of the infected population showed that the main age category concerned 5-9 year-old children with a sex ratio (Male/Female) of 1.97. Data concerning the geographic origin of the family revealed a majority of Belgian nationality (44.7%), suggesting that the contamination has originated in Belgium. Other nationalities primarily originated from Africa. At this time, no clear correlation has been established between one particular strain and a specific country of origin. [less ▲]

Detailed reference viewed: 85 (9 ULiège)
Full Text
See detailRapport du Centre National de Référence Mycoses Liège 2015
SACHELI, Rosalie ULiege; HAYETTE, Marie-Pierre ULiege

Book published by ISP-WIV (2016)

Au cours de l’année 2015, tous les aspects des missions attribuées au CNR ont été couverts, comme l’identification d’isolats de levures et champignons filamenteux, l’aide au diagnostic de mycoses rares ... [more ▼]

Au cours de l’année 2015, tous les aspects des missions attribuées au CNR ont été couverts, comme l’identification d’isolats de levures et champignons filamenteux, l’aide au diagnostic de mycoses rares, la détermination de la sensibilité aux antifongiques et l’amélioration de techniques de typage et d’identification moléculaire. Le CNR de Liège se focalise principalement sur l’identification des mycoses superficielles isolées de phanères. Parmi les techniques d’identification des levures, l’identification par spectrométrie de masse (Maldi-tof) est l’outil n°1 qui est utilisé. Les résultats sont confirmés par séquençage moléculaire si nécessaire. Parmi les outils moléculaires, une approche polyphasique est utilisée. La région ciblée en premier lieu dans le cas de l’identification d’une espèce est tout ou partie de la région ITS1-5,8S-ITS2 de l’ARN ribosomique (ARNr). Deux cibles complémentaires sont disponibles à savoir la région D1-D2 de la partie LSU (28S) de l’ARNr et la bêta-tubuline. Ces autres cibles sont utilisées en cas de confirmation de l’identification d’une espèce rare ou en cas de réponse non satisfaisante après une première amplification. Le CNR Mycose a acquis l’appareil Diversilab (BioMérieux). Cet appareil permet une caractérisation précise du génome des champignons filamenteux (y compris les dermatophytes) et des levures. Il a déjà été utilisé pour la caractérisation de souches issues d’une étude nationale. Il pourrait aussi être utilisé en cas d’épidémies notamment afin de caractériser rapidement le génome de la souche circulante. [less ▲]

Detailed reference viewed: 54 (0 ULiège)
Full Text
Peer Reviewed
See detailEpidemiological aspects and genotypic characterization of T.violaceum strains collected during a Belgian National survey on anthropophilic tinea
SACHELI, Rosalie ULiege; Dekkers, Charlotte; GRAIDE, Hélène ULiege et al

in Mycoses (2015, October), 58(Supplement 4), 189

Objectives The last two years, clinical cases of tinea capitis caused by Trichophyton violaceum (T. violaceum), have been identified in Belgium. To better understand the emergence of this species in the ... [more ▼]

Objectives The last two years, clinical cases of tinea capitis caused by Trichophyton violaceum (T. violaceum), have been identified in Belgium. To better understand the emergence of this species in the population, the Belgian National Reference Center (NRC Liège) launched a one-year national survey in 2013. Epidemiological aspects and genotypic characterization of the strains were included. Methods The study was conducted from March 2013 up to February 2014. All Belgian laboratories were asked to send M. audouinii and T. violaceum strains isolated from hair to the NRC with a form to fill in including epidemiological data. The fungal strains were identified by microscopy or ITS sequencing in case of doubtful identification. The genotypic analysis was performed by the DiversiLab® system (bioMérieux) for DNA fingerprinting and analysis. Epidemiological data were analyzed with the help of a biostatistician. Results Amongst the collected isolates, 23 strains were confirmed as T.violaceum (results concerning the 116 M. audouinii strains have already been reported). Analysis of the epidemiological characteristics of the infected population shows that the main age category concerns 0-4 year-old children (n=9, 39,1%) with a sex-ratio M/F of 1.875. Data concerning the geographic origin of the family were present in 82,6% of the cases and reveal that patients were mainly of Ethiopian origin (n=8, 57,9% of known cases). One patient was also from Burundi showing that T. violaceum strains probably circulate mainly in East Africa. The genotypic analysis led to the distinction of 2 variants of T. violaceum. The major group was composed of 17 strains which were mainly collected in the North of Belgium and included also the reference strain (18/23, 83,3%). The other group (6 strains) was close to the major group but the analysis of the spectral superposition showed some differences between these two groups, defining two distinct variants of T. violaceum in the Belgian population. This second variant was mainly recovered from South Belgium (5/6, 83,3%). No correlation could be made between the genotypic group and a particular ethnical origin as Ethiopian subjects were found in both groups. Conclusion The DiversiLab® system proved to be an efficient method to investigate the molecular epidemiology of dermatophytes infections as reported previously for M. audouinii. These results show that two distinct isolates co-exist in Belgium providing evidence of genetic heterogeneity and a possible spread of one genotypic variant in a restricted geographic area or the co-existence of two variants circulating in different African communities. However, no clear correlation could be established between the appartenance to a group and epidemiological factors, such as age or ethnical origin. [less ▲]

Detailed reference viewed: 59 (18 ULiège)
Full Text
Peer Reviewed
See detailA three year survey of dermatophytoses in Belgium
SACHELI, Rosalie ULiege; DARFOUF, Rajae ULiege; GRAIDE, Hélène ULiege et al

in Mycoses (2015, October), 58(Supplement 4), 135

Objectives Dermatophytosis refers to superficial fungal infections of keratinized tissues caused by keratinophilic dermatophytes. They are the most common cause of superficial fungal infections worldwide ... [more ▼]

Objectives Dermatophytosis refers to superficial fungal infections of keratinized tissues caused by keratinophilic dermatophytes. They are the most common cause of superficial fungal infections worldwide. Epidemiological studies regarding dermatophyte infections have been conducted in several countries and differences in the incidence and in etiological agents have been reported for different geographical areas. That is why national surveillance of circulating strains causing dermatophytosis is crucial. The Belgian National Reference Center (NRC) for Mycoses conducted a survey on dermatophytes strains circulating from 2012 to 2014. The present study was performed to assess the profile of dermatophytosis and to identify the species involved. Methods The Belgian NRC for Mycosis collected 9138 strains between January 2012 and December 2014. The isolates were cultured from patients clinically suspected for fungal infections of skin, hair and nails. Isolates were sent by Belgian laboratories to the two labs of the Belgian NRC (UZ Leuven and CHU of Liège) in order to identify the fungus or to confirm the identification. All isolates cultured from patients of UZ Leuven and CHU of Liège were also included. Fungal identification was performed by microscopy after subculture and in case of doubtful identifications by ITS sequencing. Results .Among the 9138 samples (results of UZ Leuven and CHU of Liège combined), 3587 were identified as dermatophytes. Trichophyton. rubrum (T. rubrum) was the most prevalent species accounting for 56,17% (n=2015) of the infections from all sources, followed by T. mentagrophytes complex (21,83%, n=783). The other main etiological agents of dermatophytosis recorded in this study in descending order of prevalence were M. audouinii (n=303), M. canis (n=120), T. violaceum (n=112), T. tonsurans (n= 95), T. soudanense (n=66), M. praecox (n=59), E. floccosum (n=14) Our data reveal the predominance of anthropophilic species causing tinea capitis especially M. audouinii responsible for 36,49% (n=163/448) of hair/scalp infection. Trichophyton violaceum rarely observed in our country is frequently found as 12,8% (n=57) of the reported cases of tinea capitis are due to this species. The retrospective evaluation of data collected also shows that zoophilic strains as M. canis well represented in the past epidemiology of tinea capitis, is decreasing in frequency accounting for only 7,2% (n=32) of clinical cases. Finally, our data confirm the high prevalence of T. rubrum commonly observed in Europe as causal agent of onychomycosis (70,9%, n=1603) followed by T. mentagrophytes complex (20,9%, n=455). T. rubrum and T. mentagrophytes complex are also responsible for the majority of skin infections as they represent respectively 40% (n=386) and 24,75% (n=239) of skin dermatophytosis during the study period. Conclusions The present work has provided recent data on the prevalence of several dermatophytes species circulating in Belgium. Such data is critical for the establishment of therapeutic strategies and measures for prevention and control of dermatophytes infections. Our study confirms the predominance of T. rubrum followed by T. mentagrophytes in the Belgian population but also highlights the emergence of new anthropophilic species such as M. audouinii and T. violaceum as causative agents of tinea capitis in children in relation with African immigration. [less ▲]

Detailed reference viewed: 83 (11 ULiège)
Full Text
Peer Reviewed
See detailDermatophytosis, Trends in Epidemiolgy and Diagnostic Approach
HAYETTE, Marie-Pierre ULiege; SACHELI, Rosalie ULiege

in Current Fungal Infections report (2015), 9(3), 164-179

Dermatophytes are among the common fungal agents implicated in superficial skin infections. The anthropophilic dermatophyte Trichophyton rubrum is still the most frequent causative agent worldwide but the ... [more ▼]

Dermatophytes are among the common fungal agents implicated in superficial skin infections. The anthropophilic dermatophyte Trichophyton rubrum is still the most frequent causative agent worldwide but the prevalence of several species of dermatophytes varies through different areas around the world. This review summarizes the current status of dermatophytes infection in Europe, Africa, Asia and America and gives an overview of the molecular biology laboratory methods currently available for the diagnosis of dermatomycoses. [less ▲]

Detailed reference viewed: 72 (18 ULiège)
Full Text
See detailApplication du séquençage pour l’identification des champignons
SACHELI, Rosalie ULiege

Learning material (2015)

Detailed reference viewed: 31 (7 ULiège)
Full Text
Peer Reviewed
See detailEpidemiological aspects and genotypic characterization of strains of Microsporum audouinii isolated in the context of a Belgian National survey on anthropophilic tinea
SACHELI, Rosalie ULiege; Dekkers, Charlotte; DARFOUF, Rajae ULiege et al

Poster (2015, May)

Objectives The last two years, clinical cases of tinea capitis caused by Microsporum audouinii (M. audouinii), have increased in Belgium. To better understand the emergence of this species in the ... [more ▼]

Objectives The last two years, clinical cases of tinea capitis caused by Microsporum audouinii (M. audouinii), have increased in Belgium. To better understand the emergence of this species in the population, the Belgian National Reference Center (NRC) for dermatophytes launched a national survey in 2013. Epidemiological aspects and genotypic characterization of the strains were included. Methods The study was conducted from March 2013 up to February 2014. All Belgian laboratories were asked to send M. audouinii strains isolated from hair to the NRC with a form to fill in including epidemiological informations. The fungal strains were identified by microscopy or ITS sequencing in case of doubt. The genotypic analysis was performed by the DiversiLab® system (bioMérieux) for DNA fingerprinting and analysis. Epidemiological informations were analyzed with the help of a biostatistician. Results Among the collected isolates, 117 strains have been currently confirmed as M. audouinii. Analysis of the epidemiological characteristics of the infected population shows that the main age category concerns 5-9 year-old children (64%, p< 0,0001) with a sex-ratio M/F of 1.97. Data concerning the geographic origin of the family have been obtained in only 33,6% of the cases. It reveals that strains have been mainly isolated from patients with a Belgian nationality (43,6%) suggesting bias in the data collection. The geographic origin of the remaining group includes several African countries such as Congo (20,61%), Guinea (12,8%) and Burundi (5,12%). The genotypic analysis led to the distinction of 6 genotypic variants of M. audouinii. One of these variants was exclusively recovered from South Belgium (11 strains). The major group was composed of 96 strains, well distributed in different Belgium locations. Two other groups of three strains each were close to the major group but the analysis of the spectral superposition showed some differences between these groups. The two last groups were clearly different from the major group but species identification was confirmed by ITS sequencing. Conclusion The results of the genomic analysis by Diversilab, show that several groups of M. audouinii isolates co-exist in Belgium providing evidence of genetic heterogeneity. However, no clear correlation could be established between the appartenance to a group and epidemiological factors, such as the age or ethnical origin. ________________________________________ [less ▲]

Detailed reference viewed: 75 (13 ULiège)
Full Text
Peer Reviewed
See detailEpidemiological aspects and genotypic characterization of strains of Microsporum audouinii isolated in the context of a Belgian National survey on anthropophilic tinea
SACHELI, Rosalie ULiege; Géron, Bénédicte; Dekkers, Charlotte et al

Poster (2015, April 28)

Objectives The last two years, clinical cases of tinea capitis caused by Microsporum audouinii (M. audouinii), have increased in Belgium. To better understand the emergence of this species in the ... [more ▼]

Objectives The last two years, clinical cases of tinea capitis caused by Microsporum audouinii (M. audouinii), have increased in Belgium. To better understand the emergence of this species in the population, the Belgian National Reference Center (NRC) for dermatophytes launched a national survey in 2013. Epidemiological aspects and genotypic characterization of the strains were included. Methods The study was conducted from March 2013 up to February 2014. All Belgian laboratories were asked to send M. audouinii strains isolated from hair to the NRC with a form to fill in including epidemiological informations. The fungal strains were identified by microscopy or ITS sequencing in case of doubt. The genotypic analysis was performed by the DiversiLab® system (bioMérieux) for DNA fingerprinting and analysis. Epidemiological informations were analyzed with the help of a biostatistician. Results Among the collected isolates, 97 strains have been currently confirmed as M. audouinii. Preliminary analysis of the epidemiological characteristics of the infected population shows that the main age category concerns 5-9 year-old children (84%) with a sex-ratio M/F of 1.95. Data concerning the geographic origin of the family have been obtained in only 45.8% of the cases. It reveals that strains have been mainly isolated from patients with a Belgian nationality (77%) suggesting bias in the data collection. The geographic origin of the remaining group (23%) includes several African countries. The genotypic analysis led to the distinction of 3 genotypic variants of M. audouinii. One of these variants was exclusively recovered from South Belgium (11 strains). The major group was composed of 85 strains, well distributed in different Belgium locations. The last group contains only one strain but this strain was significantly different from the two other variants. Conclusion The automated typing DiversiLab® system proved to be an easy and efficient method to investigate the molecular epidemiology of dermatophytes infections. These preliminary results show that, through Belgium, several groups of isolates co-exist for M. audouinii providing evidence of genetic heterogeneity. At this time all epidemiological informations have not yet been assessed while 35 strains of M. audouinii remain to be analysed genotypically to give definitive conclusions. [less ▲]

Detailed reference viewed: 49 (12 ULiège)
Full Text
See detailRapport du Centre National de Référence Mycoses Liège 2014
SACHELI, Rosalie ULiege; Hayette, Marie-Pierre ULiege

Book published by ISP-WIV (2015)

Au cours de l’année 2014, tous les aspects des missions attribuées au CNR ont été couverts, comme l’identification d’isolats de levures et champignons filamenteux, l’aide au diagnostic de mycoses rares ... [more ▼]

Au cours de l’année 2014, tous les aspects des missions attribuées au CNR ont été couverts, comme l’identification d’isolats de levures et champignons filamenteux, l’aide au diagnostic de mycoses rares, la détermination de la sensibilité aux antifongiques et l’amélioration de techniques de typage et d’identification moléculaire. Le CNR de Liège se focalise principalement sur l’identification des mycoses superficielles isolées de phanères. Parmi les techniques d’identification des levures, l’identification par spectrométrie de masse est l’outil n°1 qui est utilisé. Les résultats sont confirmés par séquençage moléculaire si nécessaire. Parmi les outils moléculaires, une approche polyphasique est utilisée. La région ciblée en premier lieu dans le cas de l’identification d’une espèce est tout ou partie de la région ITS1-5,8S-ITS2 de l’ARN ribosomique (ARNr). Deux cibles complémentaires sont disponibles à savoir la région D1-D2 de la partie LSU (28S) de l’ARNr et la bêta-tubuline. Ces autres cibles sont utilisées en cas de confirmation de l’identification d’une espèce rare ou en cas de réponse non satisfaisante après une première amplification. D’autres cibles sont actuellement à l’étude. Le CNR Mycose a acquis en 2014 l’appareil Diversilab® (BioMérieux). Cet appareil permet une caractérisation précise du génome des champignons filamenteux (y compris les dermatophytes) et des levures. Il a été déjà utilisé afin de caractériser les souches de dermatophytes anthropophile circulant en Belgique en 2013. Il pourrait être utilisé en cas d’épidémie notamment afin de caractériser rapidement le génome de la souche circulante. [less ▲]

Detailed reference viewed: 19 (1 ULiège)