An immuno-PF2D-MS/MS proteomic approach for bacterial antigenic characterization: To Bacillus and beyondRuelle, Virginie ; ; Elmoualij, Benaïssa et alin Journal of Proteome Research (2007), 6(6), 2168-2175 We are confronted daily to unknown microorganisms that have yet to be characterized, detected, and/ or analyzed. We propose, in this study, a multidimensional strategy using polyclonal antibodies ... [more ▼] We are confronted daily to unknown microorganisms that have yet to be characterized, detected, and/ or analyzed. We propose, in this study, a multidimensional strategy using polyclonal antibodies, consisting of a novel proteomic tool, the ProteomeLab PF2D, coupled to immunological techniques and mass spectrometry ( i-PF2D-MS/MS). To evaluate this strategy, we have applied it to Bacillus subtilis, considered here as our unknown bacterial model. [less ▲] Detailed reference viewed: 34 (10 ULg) Advances in immunoproteomics for serological characterization of microbial antigens; Ruelle, Virginie ; Elmoualij, Benaïssa et alin Journal of Microbiological Methods (2006), 67(3), 593-596 We propose a multi-dimensional strategy, associating immunodetection to a protein fractionating two-dimensional liquid chromatography tool, for serological characterization of microbial antigens. The ... [more ▼] We propose a multi-dimensional strategy, associating immunodetection to a protein fractionating two-dimensional liquid chromatography tool, for serological characterization of microbial antigens. The originality of such immunoproteomic approaches resides in their application in large-scale studies for rapid serotyping of micro-organisms, evaluation of immunomes and could be proposed in the development and monitoring of vaccines. (c) 2006 Elsevier B.V. All rights reserved. [less ▲] Detailed reference viewed: 16 (1 ULg) |
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