References of "Rigali, Sébastien"
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See detailStreptomyces from Moonmilk, an Untapped Source of New Bioactive Compounds
Martinet, Loïc ULiege; Maciejewska, Marta; Adam, Delphine ULiege et al

Poster (2017, May 24)

Moonmilk speleothems of limestone caves host a rich microbiome, among which Actinobacteria represent one of the most abundant phyla. Ancient medical texts reported that moonmilk had therapeutical ... [more ▼]

Moonmilk speleothems of limestone caves host a rich microbiome, among which Actinobacteria represent one of the most abundant phyla. Ancient medical texts reported that moonmilk had therapeutical properties, thereby suggesting that its filamentous endemic actinobacterial population might be a source of natural products useful in human treatment. In this work, a screening approach was undertaken in order to isolate cultivable Actinobacteria from moonmilk of the Grotte des Collemboles in Belgium, to evaluate their taxonomic profile, and to assess their potential in biosynthesis of antimicrobials. Phylogenetic analysis revealed that all 78 isolates were exclusively affiliated to the genus Streptomyces and clustered into 31 distinct phylotypes displaying various pigmentation patterns and morphological features. Phylotype representatives were tested for antibacterial and antifungal activities and their genomes were mined for secondary metabolite biosynthetic genes coding for non-ribosomal peptide synthetases (NRPSs), and polyketide synthases (PKS). The moonmilk Streptomyces collection was found to display strong inhibitory activities against a wide range of reference organisms, as 94, 71, and 94% of the isolates inhibited or impaired the growth of Gram-positive, Gram-negative bacteria, and fungi, respectively. Interestingly, 90% of the cave strains induced strong growth suppression against the multi-drug resistant Rasamsonia argillacea, a causative agent of invasive mycosis in cystic fibrosis and chronic granulomatous diseases. We are currently identifying molecules responsible for the observed antimicrobial activities which, next to few known bioactive compounds, also reveals many active compounds with molecular masses that do not correspond to known antibiotic deposited in web biomolecules databases such as KNapSAcK, Pubchem, Chemspider. The challenge is to increase the production yields of these unknown compounds, and purify them to further characterize their structure by NMR. Overall, our work supports the common belief that moonmilk might effectively treat various infectious diseases thanks to the presence of a highly diverse population of prolific antimicrobial producing Streptomyces, and thus may indeed constitute a promising reservoir of potentially novel active natural compounds. [less ▲]

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See detailTracking the Subtle Mutations Thriving Host Sensing by the Plant Pathogen Streptomyces scabies
Deflandre, Benoit ULiege; Jourdan, Samuel ULiege; Francis, Isolde Maria et al

Poster (2017, January 12)

Le modèle de la pathogénicité végétale chez les Streptomyces, S. scabies est responsable de la maladie de la galle commune. La thaxtomine est la phytotoxine qui cause cette maladie. Il y a peu, la voie de ... [more ▼]

Le modèle de la pathogénicité végétale chez les Streptomyces, S. scabies est responsable de la maladie de la galle commune. La thaxtomine est la phytotoxine qui cause cette maladie. Il y a peu, la voie de production de la toxine a été découverte : celle-ci met en oeuvre un double mécanisme de régulation génétique. Cependant, certains aspects de la perception de son hôte par le pathogène sont toujours inconnus. Dans ce poster, nous proposons plusieurs hypothèses pour expliquer comment quelques éléments subtiles peuvent être à l'origine de ce mécanisme de pathogénicité. [less ▲]

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See detailAssessment of the Potential Role of Streptomyces in Cave Moonmilk Formation
Maciejewska, Marta; Adam, Delphine; Naomé, Aymeric ULiege et al

in Frontiers in Microbiology (2017), 8

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See detailTracking the Subtle Mutations Driving Host Sensing by the Plant Pathogen Streptomyces scabies
Jourdan, Samuel; Francis, Jourdan; Deflandre, Benoit et al

in mSphere (2017)

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See detailPREDetector 2.0: Online and Enhanced Version of the Prokaryotic Regulatory Elements Detector Tool
Tocquin, Pierre ULiege; Naomé, Aymeric ULiege; Jourdan, Samuel ULiege et al

E-print/Working paper (2016)

In the era that huge numbers of microbial genomes are being released in the databases, it becomes increasingly important to rapidly mine genes as well as predict the regulatory networks that control their ... [more ▼]

In the era that huge numbers of microbial genomes are being released in the databases, it becomes increasingly important to rapidly mine genes as well as predict the regulatory networks that control their expression. To this end, we have developed an improved and online version of the PREDetector software aimed at identifying putative transcription factor-binding sites (TFBS) in bacterial genomes. The original philosophy of PREDetector 1.0 is maintained, i.e. to allow users to freely fix the DNA-motif screening parameters, and to provide a statistical means to estimate the reliability of the prediction output. This new version offers an interactive table as well as graphics to dynamically alter the main screening parameters with automatic update of the list of identified putative TFBS. PREDetector 2.0 also has the following additional options: (i) access to genome sequences from different databases, (ii) access to weight matrices from public repositories, (iii) visualization of the predicted hits in their genomic context, (iv) grouping of hits identified in the same upstream region, (v) possibility to store the performed jobs, and (vi) automated export of the results in various formats. PREDetector 2.0 is available at http://predetector.fsc.ulg.ac.be/. [less ▲]

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See detailIntertwining nutrient-sensory networks and the control of antibiotic production in Streptomyces.
Urem, M; Świątek-Połatyńska, M; Rigali, Sébastien ULiege et al

in Molecular Microbiology (2016)

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See detailThe CebE/MsiK Transporter is a Doorway to the Cello-oligosaccharide-mediated Induction of Streptomyces scabies Pathogenicity
Jourdan, Samuel ULiege; Francis, Isolde; Kim, Min et al

in Scientific Reports (2016)

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See detailOsdR of Streptomyces coelicolor and the Dormancy Regulator DevR of Mycobacterium tuberculosis Control Overlapping Regulons
Urem, Mia; van Rossum, Teunke; Bucca, Giselda et al

in mSystems (2016)

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See detailThe alarming increase in antibiotic-resistant bacteria
Frère, Jean-Marie ULiege; Rigali, Sébastien ULiege

in Drug Target review (2016)

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See detailOnset of Streptomyces development: an iron fist dictatorship
Rigali, Sébastien ULiege

Conference (2015, December 03)

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See detailComputational Prediction of Regulatory Networks linked to Secondary Metabolite Production
Rigali, Sébastien ULiege

Scientific conference (2015, September)

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See detailRésistance aux antibiotiques : le retour à l’ère prébiotique ?
Galleni, Moreno ULiege; Kerff, Frédéric ULiege; Rigali, Sébastien ULiege

Conference (2015, February 05)

Aujourd’hui, la résistance des bactéries aux antibiotiques relève d'une préoccupation croissante dans le domaine de la santé publique et s'impose plus que jamais comme une priorité. Ce problème a un coût ... [more ▼]

Aujourd’hui, la résistance des bactéries aux antibiotiques relève d'une préoccupation croissante dans le domaine de la santé publique et s'impose plus que jamais comme une priorité. Ce problème a un coût, sociétal et économique en Europe: 25.000 décès annuels par septicémie et 1,5 milliard d’euros d’augmentation du coût des traitements. Bien que le besoin de nouveaux agents antibactériens en milieu clinique est urgent, seules deux nouvelles classes d’antibiotiques ont pu être proposées durant ces 30 dernières années. En effet, la découverte et le développement de nouveaux antibiotiques posent des défis scientifiques, cliniques et financiers importants. [less ▲]

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See detailGenome-Wide Analysis of In Vivo Binding of the Master Regulator DasR in Streptomyces coelicolor Identifies Novel Non-Canonical Targets
Świątek-Połatyńska, MA; Bucca, G; Laing, E et al

in PLoS ONE (2015)

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See detailGrowth of desferrioxamine deficient Streptomyces mutants through xenosiderophore piracy of airborne fungal contaminations
Arguelles Arias, Anthony ULiege; Lambert, Stephany; Martinet, Loïc et al

in FEMS Microbiology Ecology (2015)

Detailed reference viewed: 69 (17 ULiège)
See detailPharmacie Souterraine et Richesse du Karst Wallon
Rigali, Sébastien ULiege

Conference (2015)

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See detailDasR is a pleiotropic regulator required for antibiotic production, pigment biosynthesis, and morphological development in Saccharopolyspora erythrae
Liao, Cheng-Heng; Xu, Ya; Rigali, Sébastien ULiege et al

in Applied Microbiology and Biotechnology (2015)

Detailed reference viewed: 23 (3 ULiège)