References of "Richel, Aurore"
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See detailLes aspects stratégiques du bioraffinage de seconde génération en Région Wallonne : symbiose industrielle ou autonomie complète ?
Jacquet, Nicolas ULg; Istasse, Thibaut ULg; Berchem, Thomas ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (in press)

Introduction. Le bioraffinage s’impose progressivement, à l’échelle commerciale, comme complément ou alternative aux filières de production traditionnelles. Littérature. A ce jour, les principaux sites ... [more ▼]

Introduction. Le bioraffinage s’impose progressivement, à l’échelle commerciale, comme complément ou alternative aux filières de production traditionnelles. Littérature. A ce jour, les principaux sites industriels de bioraffinage s’orientent vers la production de biocarburants (bioéthanol ou biodiésel). Le territoire wallon, de par son accès limité aux ressources végétales renouvelables, semble cependant s’orienter spécifiquement et compétitivement vers la production à plus faibles tonnages de bioproduits. Conclusions. Afin de mener à bien cette initiative, cette étude propose d’argumenter le meilleur choix stratégique devant être ciblé par la Wallonie: développer de nouveaux sites de production autonomes (énergétiquement suffisants et avec des filières d’approvisionnement indépendantes) ou s’ancrer à des systèmes ou des installations de production existants (et bénéficier des facilités déjà opérationnelles d’accès à l’énergie et aux matières premières). Le design de nouvelles unités de bioraffinage intégrées à des unités de production existantes semble être la meilleure option à définir pour le territoire wallon. [less ▲]

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See detailMonitoring the laccase reaction of vanillin and poplar hydrolysate
Sòti, Valentin; Jacquet, Nicolas ULg; Apers, Sandra et al

in Journal of Chemical Technology & Biotechnology (in press)

BACKGROUND: Laccase is an intensively researched enzyme for industrial use. Except for decolorisation measurements, HPLC analysis is the conventional method for monitoring the phenolic removal during ... [more ▼]

BACKGROUND: Laccase is an intensively researched enzyme for industrial use. Except for decolorisation measurements, HPLC analysis is the conventional method for monitoring the phenolic removal during laccase enzyme reaction. This paper reports an investigation of the continuous UV absorbance follow-up of the laccase reaction with steam pretreated poplar hydrolysate. RESULTS: Vanillin was used as a model substrate and lignocellulose xylose rich fraction (XRF) as a biologically complex substrate for laccase detoxification. The reaction was followed by HPLC-UV as well as by UV spectrometric measurements. Results suggest that the reaction can be successfully monitored by measuring the change of UV absorbance at 280 nm, without previous compound separation. In case of XRF experiments the spectrophotometric follow-up is especially useful, as HPLC analysis takes a long time and provides less information than in case of single substrates. The method seems to be suitable for optimization and process control. CONCLUSION: The obtained results can help to construct a fast, easy and straightforward monitoring system for laccase-phenolic substrate reactions. [less ▲]

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See detailScalable temperature induced stress for the large-scale production of functionalized Bifidobacteria
Nguyen, Huu Thanh ULg; Razafindralambo, Hary; Richel, Aurore ULg et al

in Journal of Industrial Microbiology & Biotechnology (in press)

The application of sub-lethal stresses is known to be an efficient strategy to enhance survival of probiotic bacteria during drying processes. In this context, we previously showed that the application of ... [more ▼]

The application of sub-lethal stresses is known to be an efficient strategy to enhance survival of probiotic bacteria during drying processes. In this context, we previously showed that the application of heat stress upon the entry into stationary phase increased significantly the viability of Bifidobacterium bifidum. However, this heat shock has been considered only in small scale bioreactor and no information is available for a possible scaling-up strategy. Five different operating scales (0.2 L, 2 L, 20 L, 200 L and 2000 L) have thus been tested and the results showed that the viability of B. bifidum increases from 3.15 to 6.57 folds, depending on the scale considered. Our observations pointed out the fact that the heat stress procedure is scalable according to the main outcome, i.e. increases in cell viability, but other factors have to be taken into account. Among these factors, dissolved carbon dioxide seems to play a significant role since it explain the differences observed between the test performed at lab-scale and in industrial conditions. [less ▲]

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See detailBiochemical characterization of the oil of durum wheat germ (Tunisian variety)
Kammoun, Maroua; Rassâa, Neila; Mejri-Gaïda, Myriam et al

in Journal of New Sciences (in press)

The wheat germ oil may constitute an alternative to the misuse of this cereal grain resource. The Tunisian exploitation is limited, at present, in the human and animal feed in the grain form, straw or ... [more ▼]

The wheat germ oil may constitute an alternative to the misuse of this cereal grain resource. The Tunisian exploitation is limited, at present, in the human and animal feed in the grain form, straw or some transformed products. For the purpose of characterization, the wheat germ oil has been extracted by Soxhlet apparatus from a Tunisian variety of durum wheat (Maali) based on the hexane as a solvent. An analysis by gas chromatography (GC) was then performed to identify and quantify the fatty acids of the oil. Then, the oil has submitted to a second chromatography analysis in liquid phase (HPLC) to quantify the tocopherols. The most important fatty acids constituting have been the two polyunsaturated acids linoleic acid C18:2 (OMEGA 6) and the gamma acid- linoleic C18:3 : 58.24 % and 4.19 % respectively; and the acid monounsaturated oleic acid C18:1 ( 24.49 %) but also some saturated acid: palmitic acid C16:0 with ( 19.50 % ), and the stearic acid C18:0 (1.29%). For this variety of Tunisian durum wheat, the wheat germ oil contains, in addition, 80.27 mg / 100g of α- tocopherols (vitamin E). The wheat germ oil extracted from the Tunisian variety 'Maali' has proved rich in vitamin E and omega-6 ; a better valorisation on the nutritional or pharmaceutical plan is to this title recommended. [less ▲]

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See detailLa Chimie Biologique Industrielle à Gembloux Agro-Bio Tech
Berchem, Thomas ULg; Istasse, Thibaut ULg; Schmetz, Quentin ULg et al

Conference given outside the academic context (2016)

Présentation du laboratoire de Chimie Biologique Industrielle et illustration de nos principales activités; point sur les recherches de trois jeunes doctorants. Leur travail consiste en la valorisation de ... [more ▼]

Présentation du laboratoire de Chimie Biologique Industrielle et illustration de nos principales activités; point sur les recherches de trois jeunes doctorants. Leur travail consiste en la valorisation de matrices biologiques (déchets agricoles, industriels et forestiers,...) pour produire une gamme de nouveaux produits, biocarburants ou molécules chimiques. [less ▲]

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See detailProduction de nanocellulose à partir de pâte à papier: Hydrolyse enzymatique et valorisation des coproduits dans une stratégie de bioraffinage forestier intégré
Bombeck, Pierre-Louis ULg; Richel, Aurore ULg; Jacquet, Nicolas ULg et al

Conference (2016, January 19)

Présentation succincte de la thématique de recherche doctorale et de son contexte

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See detailEtude du potentiel des sous-produits vinicoles pour des applications à haute valeur ajoutée
Berchem, Thomas ULg; Jacquet, Nicolas ULg; Richel, Aurore ULg

Conference (2016, January 19)

Grape growing is one of the largest crops around world. Grape pomaces seem to be an interesting source of bio-based molecules but, despite this potential, these byproducts are untapped. Their uses are ... [more ▼]

Grape growing is one of the largest crops around world. Grape pomaces seem to be an interesting source of bio-based molecules but, despite this potential, these byproducts are untapped. Their uses are limited to poor value added applications like composting, agricultural spreading or bioethanol. This work focuses on high value added valorisation possibilities of grape pomace. It takes part in the current trend of using natural bioactive molecules in pharmaceutical, cosmetic or food industry, through the extraction of polyphenols. Firstly, chemical characterization is performed on grape pomace from two grape varieties (Cabernet sauvignon and Pinot noir) in order to determine variability. Secondly, the same approach is applied to grape seeds and skins. Finally, a method of extraction of polyphenols is developed. Results shown in this work tend to indicate a high valorisation potential of grape pomace thank to the identification of compounds like bioactive molecules and especially polyphenols (0.7% in seeds and 0.4% in skins) as well as high concentrations of other compounds in seeds (lignin (43-53%), grape oil (12%), proteins (12%)) and in skins (lignin (20%), tartaric acid (4%), proteins (10%)) of which the valorisation potential from wine-making byproducts needs to be explored. [less ▲]

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See detailComprehensive comparison of the chemical and structural characterization of landfill leachate and leonardite humic fractions
Tahiri, Abdelghani ULg; Richel, Aurore ULg; Destain, Jacqueline ULg et al

in Analytical and Bioanalytical Chemistry (2016), 408(7), 1917-1928

Humic substances (HS) are complex and heterogeneous mixtures of organic compounds that occur everywhere in the environment. They represent most of the dissolved organic matter in soils, sediments (fossil ... [more ▼]

Humic substances (HS) are complex and heterogeneous mixtures of organic compounds that occur everywhere in the environment. They represent most of the dissolved organic matter in soils, sediments (fossil), water, and landfills. The exact structure of HS macromolecules has not yet been determined because of their complexity and heterogeneity. Various descriptions of HS are used depending on specific environments of origin and research interests. In order to improve the understanding of the structure of HS extracted from landfill leachate (LHS) and commercial HS from leonardite (HHS), this study sought to compare the composition and characterization of the structure of LHS and HHS using elemental composition, chromatographic (high-performance liquid chromatography (HPLC)), and spectroscopic techniques (UV–vis, FTIR, NMR, and MALDI-TOF). The results showed that LHS molecules have a lower molecular weight and less aromatic structure than HHS molecules. The characteristics of functional groups of both LHS and HHS, however, were basically similar, but there was some differences in absorbance intensity. There were also less aliphatic and acidic functional groups and more aromatic and polyphenolic compounds in the humic acid (HA) fraction than in the fulvic acid (FA) and other molecules (OM) fractions of both origins. The differences between LHS and HHS might be due to the time course of humification. Combining the results obtained from these analytical techniques cold improve our understanding of the structure of HS of different origins and thus enhance their potential use. [less ▲]

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See detailL’utilisation de l’hydrolyse enzymatique pour la production de nanocellulose dans une stratégie de bioraffinage forestier intégré
Bombeck, Pierre-Louis ULg; Hebert, Jacques ULg; Richel, Aurore ULg

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2016), 20(1), 94-103

Introduction. In a world that seeks to break free from petrochemicals, the concept of forest biomass biorefinery is increasingly being studied as a way to maximize the value of the components of this ... [more ▼]

Introduction. In a world that seeks to break free from petrochemicals, the concept of forest biomass biorefinery is increasingly being studied as a way to maximize the value of the components of this biomass. Due to the high added value expected when transforming cellulose fibers into nanocellulose, this technology is highly attractive to the pulp and paper industrial world. Literature. The concept of integrated forest biorefinery is to adapt existing pulp mills so as to maximize the value of the co-products. Through the use of various methods, two types of nanocellulose may be obtained from the cellulose in the pulp. Due to its production of valuable byproducts, enzymatic hydrolysis is an interesting method to use for this purpose, but it must be combined with mechanical post-treatments. The production of nanocellulose from chemical pulp takes place in the final step of pulp production, and could be implemented without modifying the existing mills. The economic viability of this mode of production and the potential market size are the subject of recent studies that appear encouraging. Conclusions. Nanocellulose is a promising biomaterial whose field of application continues to grow. Cellulose in wood pulp can be used as a raw material for the production of nanocellulose using enzymatic pre-treatments that generate valuable co-products. This process can be integrated into a conventional chemical pulp mill and constitutes a broadening of the products available to the pulp and paper industry. [less ▲]

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See detailCharacterization of saccharides released during an in vitro pepsin-pancreatin digestion of corn flour using HPAEC-PAD
Odjo, Djosse Psijus Sylvanus ULg; Bera, François ULg; Jacquet, Nicolas ULg et al

in Stärke = Starch (2016), 68

Saccharides released after an in vitro pepsin-pancreatin digestion of corn flour were quantified and characterized using high-performance anion-exchange chromatography coupled with a pulsed amperometric ... [more ▼]

Saccharides released after an in vitro pepsin-pancreatin digestion of corn flour were quantified and characterized using high-performance anion-exchange chromatography coupled with a pulsed amperometric detector (HPAEC-PAD) and two colorimetric approaches (glucose oxidase/ peroxidase assay and the 3,5-dinitrosalicylic acid assay). HPAEC-PAD revealed five major saccharides as the result of in vitro pepsin-pancreatin digestion of corn grain: glucose, isomaltose, maltose, maltotriose, and glucosyl-maltotriose. Concentrations of glucose released as measured by the three methods assessed are similar when the in vitro pepsin-pancreatin digestion is followed by post incubation with amyloglucosidase. This post incubation unfortunately leads to a loss of information about the degree of polymerization of oligosaccharides that can impact their absorption through intestinal tract. HPAEC-PAD gives both qualitative and quantitative information and then seems more suitable for a nutritional appreciation of the digestion of starchy feedstuffs. [less ▲]

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See detailMetabolic profile of mixed culture acidogenic fermentation of lignocellulosic residues and the effect of upstream substrate fractionation by steam explosion
Perimenis, Anastasios; van Aarle, Ingrid; Nicolay, Thomas et al

in Biomass Conversion and Biorefinery (2016), 6

Lignocellulosic biomass residues have attracted attention for the sustainable production of molecules for material and energetic use through biochemical conversion. Their recalcitrant structure prevents a ... [more ▼]

Lignocellulosic biomass residues have attracted attention for the sustainable production of molecules for material and energetic use through biochemical conversion. Their recalcitrant structure prevents a broader use and asks for the development of sustainable techniques that can efficiently separate, recover and valorize the constituting components. In a cascading concept, residual streams of such processes can be further exploited in an attempt to valorize the largest possible fraction of the initial material. Three lignocellulosic substrates, namely dried sugar beet pulp, wheat bran and miscanthus straw, were upstream fractionated by steam explosion to extract the hemicellulose fraction. This study evaluated the valorization of the residual solid fraction through mixed acidogenic fermentation for the production of volatile fatty acids (VFA) as platform chemicals. Batch experiments have been conducted for the reference material (non-treated) and the solid fraction remaining after steam explosion, with and without the addition of an external mixed inoculum. Steam explosion residues contained less hemicellulose than the initial materials. The difference in the fermentation profile between steam explosion residues and non-treated substrates is dependent on the substrate. Maximum total VFA (tVFA) concentration was 18.8 gCOD/kgmixed_liquor, and maximum yield of chemical oxygen demand (COD) conversion into tVFAwas 33 % for the case of non-treated inoculated beet pulp. [less ▲]

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See detailSteam Explosion Process
Jacquet, Nicolas ULg; Haubruge, Eric ULg; Richel, Aurore ULg

Scientific conference (2015, December 17)

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See detailIndustrial Biological Chemistry laboratory activities
Jacquet, Nicolas ULg; Richel, Aurore ULg

Conference (2015, November 26)

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See detailEffect of Metals, Metalloids and Metallic Nanoparticles on Microalgae Growth and Industrial Product Biosynthesis: A Review
Miazek, Krystian ULg; Iwanek, Waldemar; Remacle, Claire ULg et al

in International Journal of Molecular Sciences (2015), 16

Microalgae are a source of numerous compounds that can be used in many branches of industry. Synthesis of such compounds in microalgal cells can be amplified under stress conditions. Exposure to various ... [more ▼]

Microalgae are a source of numerous compounds that can be used in many branches of industry. Synthesis of such compounds in microalgal cells can be amplified under stress conditions. Exposure to various metals can be one of methods applied to induce cell stress and synthesis of target products in microalgae cultures. In this review, the potential of producing diverse biocompounds (pigments, lipids, exopolymers, peptides, phytohormones, arsenoorganics, nanoparticles) from microalgae cultures upon exposure to various metals, is evaluated. Additionally, different methods to alter microalgae response towards metals and metal stress are described. Finally, possibilities to sustain high growth rates and productivity of microalgal cultures in the presence of metals are discussed. [less ▲]

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See detailLa chimie biologique industrielle à Gembloux Agro-Bio Tech : Un aperçu de nos activités
Berchem, Thomas ULg; Istasse, Thibaut ULg; Schmetz, Quentin ULg et al

Conference given outside the academic context (2015)

Présentation du laboratoire de Chimie Biologique Industrielle et illustration de nos principales activités; point sur les recherches de trois jeunes doctorants. Leur travail consiste en la valorisation de ... [more ▼]

Présentation du laboratoire de Chimie Biologique Industrielle et illustration de nos principales activités; point sur les recherches de trois jeunes doctorants. Leur travail consiste en la valorisation de matrice biologique (déchets agricoles, industriels et forestiers,...) pour produire une gamme de nouveaux produits, biocarburants ou molécules chimiques. [less ▲]

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See detailBioresources and circular economy - Strategic approach for Wallonia
Richel, Aurore ULg

Speech/Talk (2015)

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See detailValorisation de la biomasse pour des applications vers des marchés de niche
Richel, Aurore ULg

Conference (2015, October)

Dans le contexte économique actuel de notre société, il est reconnu que des alternatives et des compléments à la pétrochimie devront être développés dans un futur proche. L’une des solutions possibles ... [more ▼]

Dans le contexte économique actuel de notre société, il est reconnu que des alternatives et des compléments à la pétrochimie devront être développés dans un futur proche. L’une des solutions possibles réside dans le développement de procédés permettant de substituer les actuels produits pétro-sourcés par de nouveaux matériaux bio-basés issus de la biomasse. Sur base de ce constat, Gembloux Agro-Bio Tech et son laboratoire de Chimie Biologique Industrielle se positionnent sur cette thématique en abordant les aspects technico-économiques de transformation du végétal en une gamme de produits à haute valeur ajoutée à destination de divers secteurs applicatifs tels que les matériaux, le cosmétique, le pharmaceutique, etc. L'importance de l'agronomie et des techniques agricoles, combinée à une gestion appropriée des déchets en circuits courts, sont deux piliers discutés dans ce exposé et illustrés au travers de divers exemples stratégiques. [less ▲]

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See detailEt si nos déchets devenaient la source de nouveaux produits. Notion d'économie circulaire
Richel, Aurore ULg; Jacquet, Nicolas ULg; Berchem, Thomas ULg et al

Conference given outside the academic context (2015)

Description et illustration de nos activités visant à utiliser les déchets agricoles et forestiers, les déchets végétaux et industriels et nos déchets ménagers pour produire une gamme de nouveaux produits ... [more ▼]

Description et illustration de nos activités visant à utiliser les déchets agricoles et forestiers, les déchets végétaux et industriels et nos déchets ménagers pour produire une gamme de nouveaux produits, biocarburants ou molécules chimiques. Cette présentation se consacre aux nouveaux produits et polymères générés au départ de ces déchets dans une approche d'économie circulaire et de priorité des usages. [less ▲]

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See detailAlternatives to traditional valorisation ways for brewer’s spent grains
Villani, Nicolas ULg; Aguedo, Mario ULg; Richel, Aurore ULg

Poster (2015, August 05)

Brewer’s Spent Grains (BSG) are a highly available and cheap food supply chain waste (FSCW) that is mainly used in low-valued feed applications. This residue represents around 85 % of the total amount of ... [more ▼]

Brewer’s Spent Grains (BSG) are a highly available and cheap food supply chain waste (FSCW) that is mainly used in low-valued feed applications. This residue represents around 85 % of the total amount of waste produced by breweries with an annual tonnage of 3.4 million tons (on a dry basis) in the European Union. Based on its composition, BSG could be valorised in a wide variety of value-added products. For example, cellulose and remaining starch could easily be turned into ethanol or used as solid state fermentation media or as platform molecules for further chemical synthesis. These alternative valorisation ways could lead to an important economic relief through the whole brewery industry. Herein is described a multistep fractionation of BSG into cellulosic pulp, free sugars, proteins, germs and lignin using an Organosolv acidic pretreatment. This extraction procedure has been optimised in order to allow the most efficient and complete valorisation of BSG. [less ▲]

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