References of "Renard, André"
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See detailStabilization of T7-Promoter-Based Parhs Expression Vectors Using the Parb Locus
De Moerlooze, L.; Struman, Ingrid ULg; Renard, Andre et al

in Gene (1992), 119(1), 91-3

We describe a modification of the pAR3040 vector which results in its efficient stabilization during cell division. The parB locus of the plasmid R1 was introduced into the plasmid, pAR3040, to construct ... [more ▼]

We describe a modification of the pAR3040 vector which results in its efficient stabilization during cell division. The parB locus of the plasmid R1 was introduced into the plasmid, pAR3040, to construct the pARHS vectors. These vectors are stable for at least 60 cell generations, even in the absence of selection by an antibiotic present in the culture media, both with or without IPTG induction. [less ▲]

Detailed reference viewed: 19 (4 ULg)
See detailRecombinant fish hormone proteins.
Rentier-Delrue, Françoise ULg; Martial, Joseph ULg; Renard, André

Patent (1990)

The present invention relates to a recombinant stable polypeptide comprising in its polypeptide chain an aminoacid sequence contained in one of the following amino sequences : * from amino acid (-20) to ... [more ▼]

The present invention relates to a recombinant stable polypeptide comprising in its polypeptide chain an aminoacid sequence contained in one of the following amino sequences : * from amino acid (-20) to amino acid (187) represented in Fig. 2, * from amino acid (1) to amino acid (187) represented in Fig. 2, * from amino acid (1) to amino acid (187) represented in Fig. 2, preceded by Met. [less ▲]

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See detailPROTEINS FOR FUSING THE SUB-UNIT B OF THE CHOLERAIC TOXIN AND HETEROLOGOUS ANTIGEN, AND NUCLEIC ACIDS ENCODING THEM.
Lhoir, Cécile; Renard, André; Martial, Joseph ULg

Patent (1990)

The invention relates to a hybrid protein which fuses the sub-unit B of the choleraic toxin with an active sequence of a heterologous antigen with respect to said unit. Said hybrid protein is comprised of ... [more ▼]

The invention relates to a hybrid protein which fuses the sub-unit B of the choleraic toxin with an active sequence of a heterologous antigen with respect to said unit. Said hybrid protein is comprised of a sequence of said sub-unit which extends between the 3rd and 100th amino acid rest of the complete sub-unit B. The heterologous amino acid sequence is used upstream or downstream of the latter. This hybrid protein is usable for vaccination, particularly to help the stabilisation of heterologous antigens in the intestinal environment. [less ▲]

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See detailSynthesis, purification and initial structural characterization of octarellin, a de novo polypeptide modelled on the alpha/beta-barrel proteins
Goraj, Karine; Renard, André; Martial, Joseph ULg

in Protein Engineering (1990), 3(4), 259-66

We have attempted to construct an artificial polypeptide that folds like the eight-stranded parallel beta-barrel structures. Our approach consists of repeating eight times a unit peptide designed to adopt ... [more ▼]

We have attempted to construct an artificial polypeptide that folds like the eight-stranded parallel beta-barrel structures. Our approach consists of repeating eight times a unit peptide designed to adopt a 'beta-strand/alpha-helix' pattern. A first 'test' sequence for this structural unit was deduced from a series of parameters defined after an analysis of three natural alpha/beta-barrel proteins and including principally the lengths of the secondary structure elements, the alpha/beta packing and the fitting on average Garnier profiles. The gene encoding this structural unit was synthesized, cloned and expressed in Escherichia coli either as a monomer or as direct repeats of 2-12 units. Preliminary structural characterization of the 7-, 8- and 9-fold unit polypeptides by circular dichroism measurements indicates the presence of the predicted amount of alpha-helix in the three proteins. Further analysis by urea-gradient gel electrophoresis demonstrates that, in the conditions tested, only the 8-fold unit polypeptide forms a compact structure through a cooperative and rapid two-state folding transition involving long-range molecular interactions. [less ▲]

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See detailCloning and expression of 3 lipase genes from an antarctic bacteria.
Feller, Georges ULg; Arpigny, Jean Louis; Thiry, Michel et al

Poster (1989)

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See detailThe Active-Site-Serine Penicillin-Recognizing Enzymes as Members of the Streptomyces R61 Dd-Peptidase Family
Joris, Bernard ULg; Ghuysen, Jean-Marie ULg; Dive, Georges ULg et al

in Biochemical Journal (1988), 250(2), 313-324

Homology searches and amino acid alignments, using the Streptomyces R61 DD-peptidase/penicillin-binding protein as reference, have been applied to the beta-lactamases of classes A and C, the Oxa-2 beta ... [more ▼]

Homology searches and amino acid alignments, using the Streptomyces R61 DD-peptidase/penicillin-binding protein as reference, have been applied to the beta-lactamases of classes A and C, the Oxa-2 beta-lactamase (considered as the first known member of an additional class D), the low-Mr DD-peptidases/penicillin-binding proteins (protein no. 5 of Escherichia coli and Bacillus subtilis) and penicillin-binding domains of the high-Mr penicillin-binding proteins (PBP1A, PBP1B, PBP2 and PBP3 of E. coli). Though the evolutionary distance may vary considerably, all these penicillin-interactive proteins and domains appear to be members of a single superfamily of active-site-serine enzymes distinct from the classical trypsin or subtilisin families. The amino acid alignments reveal several conserved boxes that consist of strict identities or homologous amino acids. The significance of these boxes is highlighted by the known results of X-ray crystallography, chemical derivatization and site-directed-mutagenesis experiments. [less ▲]

Detailed reference viewed: 36 (3 ULg)
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See detailIsolation and characterization of the human prolactin gene
Truong, Anh T; Duez, Colette ULg; Belayew, Alexandra et al

in EMBO Journal (1984), 3(2), 429-37

Prolactin (PRL) and growth hormone (GH) genes derive from a common ancestor and still share some sequence homologies. Their expression in the pituitary gland is regulated in opposite directions by most of ... [more ▼]

Prolactin (PRL) and growth hormone (GH) genes derive from a common ancestor and still share some sequence homologies. Their expression in the pituitary gland is regulated in opposite directions by most of the many hormones acting on them. This provides an interesting system to study sequences involved in gene expression. Using a human PRL cDNA clone as a probe, we screened a human genomic DNA library in lambda phage and isolated a single recombinant comprising the whole hPRL gene. It was characterized by restriction endonuclease mapping and cDNA hybridization, by DNA heteroduplex analysis and by nucleotide sequencing. The hPRL gene is present as a single copy per haploid genome, is approximately 10 kb long and contains four introns, three of which interrupt the coding sequence at the same locations as in the known GH and PRL genes. The origin of transcription was determined by S1 mapping on prolactinoma mRNAs. The search for direct and inverted repeats, as well as dyad symmetries was carried out in the 900-bp sequenced in the 5'-flanking region. Sequence homologies between hPRL, hGH and rPRL were derived from computer drawn matrices for these upstream regions. [less ▲]

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See detailLineweaver-Burk, Hanes, Eadie-Hofstee and Dixon Plots in Non-steady-state Situations.
Frère, Jean-Marie ULg; Leyh, Bernard ULg; Renard, André

in Journal of Theoretical Biology (1983), 101

Lineweaver-Burk, Hanes, Eadie-Hofstee and Dixon plots can only be used when a true initial rate is measured. Despite the fact that this point has often been stressed, it is far too often ignored in favour ... [more ▼]

Lineweaver-Burk, Hanes, Eadie-Hofstee and Dixon plots can only be used when a true initial rate is measured. Despite the fact that this point has often been stressed, it is far too often ignored in favour of restricting the essay time to one where low amounts of substrate are used. When one or several irreversible and slow steps occur with an inactivator during the incubation of a ternary enzyme-substrate-inactivator mixture, the rate of the enzyme-catalyzed reaction progressively decreases. Even under these conditions, the present computer simulations investigations show that apparently linear Lineweaver-Burk, Hanes, Eadie-Hofstee and Dixon graphs can be obtained when the amount of product is mistakenly assumed to represent the true initial rate. Moreover, the observed pattern can change with time, going for instance from non-competitive to competitive. "Ki's" measured under these conditions also vary with time and bear little relationship to the true constants involved in the interaction. [less ▲]

Detailed reference viewed: 274 (1 ULg)