References of "Remy, Benoit"
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See detailBone Turnover During Pregnancy in Horses
Greiner, Claudia; Cavalier, Etienne ULg; Remy, Benoit et al

in Journal of Bone and Mineral Research (2010), 25

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See detailAnti-mullerian hormone is an endocrine marker of ovarian gonadotropin-responsive follicles and can help to predict superovulatory responses in the cow.
Rico, Charlene; Fabre, Stephane; Medigue, Claire et al

in Biology of Reproduction (2009), 80(1), 50-59

The major limitation to the development of embryo production in cattle is the strong between-animal variability in ovulatory response to FSH-induced superovulation, mainly due to differences in ovarian ... [more ▼]

The major limitation to the development of embryo production in cattle is the strong between-animal variability in ovulatory response to FSH-induced superovulation, mainly due to differences in ovarian activity at the time of treatment. This study aimed to establish whether anti-Mullerian hormone (AMH) was an endocrine marker of follicular populations in the cow, as in human, and a possible predictor of the ovarian response to superovulation. Anti-Mullerian hormone concentrations in plasma varied 10-fold between cows before treatment and were found to be highly correlated with the numbers of 3- to 7-mm antral follicles detected by ovarian ultrasonography before treatment (r=0.79, P<0.001) and the numbers of ovulations after treatment (r=0.64, P<0.01). Between-animal differences in AMH concentrations were found to be unchanged after a 3-mo delay (r=0.87, P<0.01), indicating that AMH endocrine levels were characteristic of each animal on a long-term period. The population of healthy 3- to 7-mm follicles was the main target of superovulatory treatments, contained the highest AMH concentrations and AMH mRNA levels compared with larger follicles, and contributed importantly to AMH endocrine levels. In conclusion, AMH was found to be a reliable endocrine marker of the population of small antral gonadotropin-responsive follicles in the cow. Moreover, AMH concentrations in the plasma of individuals were indicative of their ability to respond to superovulatory treatments. [less ▲]

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See detailIntérêt de la troponine sérique en tant que marqueur d’une atteinte du myocarde en médecine vétérinaire
Sandersen, Charlotte ULg; Remy, Benoit; Olejnik, Dorine ULg et al

in Annales de Médecine Vétérinaire (2008)

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See detailDevelopment of a radioimmunoassay for bovine chymosin B
Idrissa-Sidikou, Djibo; Remy, Benoit; Dufrasne, Isabelle ULg et al

in Tropicultura (2007), 25(3), 134-139

The present study was conducted to develop and validate a specific radioimmunoassay system for measurement of bovine chymosin B (bChyB) concentrations in plasma samples. Bovine ChyB was used for ... [more ▼]

The present study was conducted to develop and validate a specific radioimmunoassay system for measurement of bovine chymosin B (bChyB) concentrations in plasma samples. Bovine ChyB was used for immunization of rabbits and as standard and tracer. Chymosin B concentrations were measured in plasma samples from two groups of calves (Group 1: calves sampled from birth to 24 hours; Group 2: calves sampled from Day 1 to 21 after birth) and from one cow during the peri-partum period. Detection limit of the assay was 9.0 ng/mL. Recovery was higher than 89.3%. Repeatability and reproducibility ranged from 1.52% to 5.23% and from 1.52% to 12.57% respectively. No cross-reaction was found with pepsinogen A from bovine, porcine or human origins. In Group 1, bChyB concentrations increased from 47.3±45.1 ng/mL (5 min after birth) to 325.5±161.2 ng/mL (12 hours after birth), then it decreased till 293.0±161.5 ng/mL (24 hours after birth). In Group 2, concentrations decreased from Day 1 (455.3±191.1 ng/mL) to Day 21 (117.9±85.1 ng/mL). In adult cow, mean concentration was 136.0±32.3 ng/mL. In conclusion, bChyB is able to cross the stomach basal membrane and to reach the blood circulation at detectable levels in both young calves and adult cows. [less ▲]

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See detailCorrelation between a proteolytic method and a radioimmunoassay for porcine serum pepsinogen concentrations
Sidikou, D. I.; Banga-Mboko, H.; Tamboura, H. H. et al

in Research in Veterinary Science (2006), 80(3), 260-266

The measurement of serum pepsinogen concentrations by enzymatic method and immunoassay provides diagnostic values and should be helpful in the detection of gastric diseases related to a rise of blood ... [more ▼]

The measurement of serum pepsinogen concentrations by enzymatic method and immunoassay provides diagnostic values and should be helpful in the detection of gastric diseases related to a rise of blood pepsinogen. In the present study, the correlation between a conventional enzymatic method and a recently developed radioimmunoassay (RIA) for serum pepsinogen A was investigated. A total of 123 sera samples of porcine foetuses (n = 28), adult healthy pigs (n = 56), pigs with parakeratosis (n = 25) and pigs with ulceration of the pars oesophagea (n = 14) were tested. Overall, there was a slight correlation between the two methods (r = 0.60). In relation to individual animal groups, the correlations (r) were 0.39 (P>0.05), 0.74 (P<0.001), 0.19 (P>0.05) and 0.34 (P>0.05) in foetuses, healthy pigs, pigs with parakeratosis and pigs with ulcers, respectively. In both methods, pepsinogen concentrations (means+/-SE) were significantly higher (P<0.05) in pigs with parakeratosis (1778 +/- 86.00 mUTyr/L; 690 +/- 53.00 ng/mL) and in pigs with ulcers (2026 +/- 153.00 mUTyr/L; 1747 +/- 94.00 ng/mL) when compared to healthy pigs (935 +/- 58.00 mUTyr/L; 275 +/- 35.00 ng/mL). The proteolytic method gave a significant increased activity (P<0.05) in foetuses (1150 +/- 82.00 mUTyr/L) vs. (935 +/- 58.00 mUTyr/L) in healthy adult pigs, indicating an additional proteolytic activity in the sera of foetuses or neonates. [less ▲]

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See detailInvestigations on PrP and PrPres in porcine brain and in stimufol preparations
Degand, Guy ULg; Remy, Benoit; Fotsing, Lucas ULg et al

in Proceedings: 20th Annual Meeting A.E.T.E (2004)

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See detailIsolation and characterization of eight pregnancy-associated glycoproteins present at high levels in the ovine placenta between day 60 and day 100 of gestation
El Amiri, B.; Remy, Benoit; Melo de Sousa, Noelita ULg et al

in Reproduction Nutrition Development (2004), 44(3, May-Jun), 169-181

Pregnancy-associated glycoproteins (PAG), structurally related to aspartic proteinases, are expressed in the outer epithelial cell layer (chorion/trophectoderm) of the ungulate placenta. The aim of the ... [more ▼]

Pregnancy-associated glycoproteins (PAG), structurally related to aspartic proteinases, are expressed in the outer epithelial cell layer (chorion/trophectoderm) of the ungulate placenta. The aim of the present study was to isolate as many PAG molecules as possible from placentae collected between day 60 and day 100 of gestation and to characterize their amino-terminal amino-acid sequences. Three heterologous radioimmunoassays were used to monitor PAG immunoreactivity throughout the isolation procedures. Sequential use of DEAE-cellulose, gel filtration, and CM ceramic chromatographies led to the isolation of several fractions rich in PAG immunoreactivity. The fractions with a large amount of proteins were also purified by chromatofocusing. The analysis of immunoreactive fractions by SDS-PAGE, Western blotting and two-dimensional electrophoresis followed by amino-terminal microsequencing on PVDF membranes allowed to identify eight different ovPAG with apparent molecular masses ranging from 55 to 66 kDa and isoelectric points from 4.0 to 6.8. The N-terminal sequences were determined and their comparison to those previously identified revealed that four of them are identical to those encoded by previously known cDNA, while the additional four sequences appear to be novel since they have not yet been described. [less ▲]

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See detailSurvey of gastric lesions and blood pepsinogen levels in pigs in Burkina Faso
Banga-Mboko, H.; Tamboura, H.; Maes, D. et al

in Veterinary Research Communications (2003), 27(8), 595-602

The purpose of the study was to investigate the prevalence of gastric lesions and to provide diagnostic values for serum pepsinogen in non-infected pigs and in pigs with gastric disease. In an abattoir ... [more ▼]

The purpose of the study was to investigate the prevalence of gastric lesions and to provide diagnostic values for serum pepsinogen in non-infected pigs and in pigs with gastric disease. In an abattoir survey, the pepsinogen concentrations were measured in the serum from 62 non-infected pigs, 33 pigs with gastric lesions and 17 pigs infected with Hyostrongylus rubidus, using a specific radioimmunoassay (RIA). The mean (+/-SE) pepsinogen concentrations in the serum of non-infected pigs, in pigs with gastric ulcers, and in pigs with a heavy H. rubidus infection were 630.8 +/- 39.2 ng/ml, 1084.5 +/- 166.2 ng/ml and 1095.2 +/- 102.3 ng/ml, respectively (p < 0.05). Because of the higher concentrations of pepsinogen in the blood of pigs with gastric ulcers or parasitic infections, it is suggested that the measurement of serum pepsinogen by RIA may be an effective biochemical approach to the diagnosis of chronic gastric disorders in pigs. [less ▲]

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See detailAn improved radioimmunoassay for measurement of pepsinogen in porcine blood samples
Banga-Mboko, Henri; Sulon, Joseph ULg; Lange, J. M. et al

in Veterinary Journal (2003), 165(3), 288-295

The study was conducted to develop a sensitive and specific radioimmunoassay (RIA) for the measurement of pepsinogen in porcine serum, and to use this test for the determination of pepsinogen ... [more ▼]

The study was conducted to develop a sensitive and specific radioimmunoassay (RIA) for the measurement of pepsinogen in porcine serum, and to use this test for the determination of pepsinogen concentrations in serum samples from fetuses and pigs of different ages. Compared to a previously described RIA, major improvements were made concerning the use of specific polyclonal antibodies and the use of an appropriate buffer. The assay was able to detect pepsinogen concentrations of >/=0.2 ng/mL. The recovery of pepsinogen was close to 95%. The intra-assay coefficients of variations ranged between 3.9 and 7.5% whereas the interassay ranged between 8.8 and 11.9%. These percentages correspond to a satisfactory accuracy and reproducibility of the assay. No cross-reactions were observed with the main commercially available products of the aspartic proteases family except porcine pepsin cross-reacted over 62.5 microg/mL. Pepsinogen concentrations increased steadily with increasing age of the fetuses and the pigs (P<0.05). Pepsinogen concentrations (+/-SE) in fetuses of 90-100 (n=24) and 100-110 days of pregnancy (n=36) were 0.5+/-0.1 and 5.3+/-1.3 ng/mL, respectively. In pigs of 21, 98, and 213 days of age, the pepsinogen concentrations were 290.6+/-10.8, 343.1+/-17.9 and 383.5+/-15.3 ng/mL, respectively. The results demonstrate that RIA is accurate and can be used easily to assess pepsinogen concentrations in pig sera. The test may constitute a valuable tool in epidemiological surveys and in studies related to gastric diseases in pigs. [less ▲]

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See detailThe use of blood pepsinogen as a biomarker of the integrity of the porcine gastric mucosa. 2. Measurements of blood pepsinogen and its usefull in the detection of gastric diseases
Banga-Mboko, Henri; Godeau, Jean-Marie ULg; Drion, Pierre ULg et al

in Annales de Médecine Vétérinaire (2003), 147(2, APR-MAY), 95-104

Pepsinogen is one component of the gastric juice which participes in the digestion. This macromolecule enters the blood circulation in a small measurable quantities in healthy subjects. Therefore, blood ... [more ▼]

Pepsinogen is one component of the gastric juice which participes in the digestion. This macromolecule enters the blood circulation in a small measurable quantities in healthy subjects. Therefore, blood pepsinogen is claimed to be an indicator of the integrity of the gastric mucosa. This paper was written to review the use of porcine in the diagnostic of stomach ulcers and Hyostrongylus rubidus infection. The methods of measurement of blood pepsinogen and the diagnostic values are discussed. [less ▲]

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See detailDevelopment and validation of a specific radioimmunoassay for equine osteocalcin
Carstanjen, Bianca; Sulon, Joseph ULg; Banga-Mboko, H. et al

in Domestic Animal Endocrinology (2003), 24(1), 31-41

This study describes for the first time the development and validation of a sensitive and specific radioimmunoassay (RIA) for equine osteocalcin (OC) quantification using purified equine OC as standard ... [more ▼]

This study describes for the first time the development and validation of a sensitive and specific radioimmunoassay (RIA) for equine osteocalcin (OC) quantification using purified equine OC as standard, tracer, and immunogen for antibody formation in rabbits. The assay allowed to measure equine serum OC levels with a sensitivity of 0.2 ng/mL. Immunoreactive serum OC values of clinically normal, different-aged horses ranged from 3.68 to 127.31 ng/mL. Intra- and inter-assay coefficients of variation (CV) were 6.2 and 8.2%, respectively. Serial equine scrum sample dilutions were linear. The recovery of equine OC from equine serum samples ranged from 93.88 to 107.9%. There was a tight correlation between OC values measured with the equine- specific OC RIA and two commercially available bovine-specific OC RIA kits. However, highest serum OC values were obtained with the equine-specific OC RIA. In conclusion, our equine-specific OC RIA is sensitive, linear, accurate, precise, and reproducible. The assay allowed to quantify OC in equine serum samples and might, therefore, be used to monitor equine osteoblast activity associated with bone diseases, exercise, therapy forms or diet. [less ▲]

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See detailIsolation and partial characterization of three pregnancy-associated glycoproteins from the ewe placenta
El Amiri, B.; Remy, Benoit; Melo de Sousa, Noelita ULg et al

in Molecular Reproduction and Development (2003), 64(2), 199-206

Pregnancy-associated glycoproteins (PAGs) are synthesized in the outer epithelial layer of the placenta in artiodactyls. In this work, three novel ovine PAGs were isolated from late-pregnancy fetal ... [more ▼]

Pregnancy-associated glycoproteins (PAGs) are synthesized in the outer epithelial layer of the placenta in artiodactyls. In this work, three novel ovine PAGs were isolated from late-pregnancy fetal cotyledons and characterized biochemically. The isolation procedure included acid and ammonium sulfate precipitations and anion and cation exchange chromatographies. The isolated PAGs have different NH2-terminal amino acid sequences (RGSXLTILPLRNMRDIVY, ISRVSXLTIHPLRNIMDML, and RGSNLTIHPLRNIRD) and apparent molecular masses (55, 57, and 59 kDa). Each shows several isoforms with different pl values. The three proteins share high sequence identity with each other and with other ovine, bovine, and caprine PAGs. They have not been described previously. The ovPAG-59 sequence differs from the previously identified ovPAG-4 sequence (determined by DNA cloning and sequencing) at only one position among the 15 N-terminal residues. The newly characterized ovPAGs and the procedure used to isolate them will be helpful in producing new antisera for investigating PAG secretion in pregnant ewes. [less ▲]

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See detailSignaux embryonnaires de la gestation
Melo de Sousa, Noelita ULg; El Amiri, B.; Remy, Benoit et al

in Journées Européennes de la Société Française de Buiatrie (2002, October 29)

Entre l’endomètre et le trophectoderme, les interactions complexes qui aboutissent à l’implantation et à la mise en place du placenta sont harmonisées par le conceptus qui envoie des signaux auxquels ... [more ▼]

Entre l’endomètre et le trophectoderme, les interactions complexes qui aboutissent à l’implantation et à la mise en place du placenta sont harmonisées par le conceptus qui envoie des signaux auxquels l’utérus répond localement ou qui contribuent à modifier l’endocrinologie maternelle (Thibault et al, 1998). Les principaux signaux embryonnaires associés à l’établissement de la gestation seront passés en revue sur base des connaissances actuelles disponibles dans la littérature scientifique. [less ▲]

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See detailCharacterization of pregnancy-associated glycoproteins extracted from zebu (Bos indicus) placentas removed at different gestational periods
Melo de Sousa, Noelita ULg; Remy, Benoit; El Amiri, Bouchra et al

in Reproduction Nutrition Development (2002), 42(3), 1-15

In the present work, two biochemical approaches were used to characterize PAGs isolated from Bos indicus fetal cotyledons removed at different gestational ages. The first procedure included acidic and ... [more ▼]

In the present work, two biochemical approaches were used to characterize PAGs isolated from Bos indicus fetal cotyledons removed at different gestational ages. The first procedure included acidic and ammonium sulfate precipitations, anion and cation exchange chromatographies and the second included pepstatin-agarose affinity chromatography. A bovine PAG radioimmunoassay was used to monitor the immunoreactivity throughout the isolation procedures. The most immunoreactive fractions issued from cation exchange and affinity chromatographies were analyzed by SDS-PAGE and Western blotting, before transfer to a polyvinylidene difluoride (PVDF) membrane for NH2-microsequence determination. Use SDS-PAGE and Western blotting, different isoforms of PAG with apparent molecular masses of 51 to 69 kDa and isoelectric points varying from 4.4 to 6.7 were identified in the placentas from different gestational ages. N-terminal microsequencing (10 to 25 aa long) indicates the expression of one single terminal amino acid sequence in the Bos indicus placenta, which is 100% identical to the bovine PAG-1. [less ▲]

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See detailMicrosequencing of three pregnancy-associated glycoproteins isolated from sheep placenta
El Amiri, Bouchra; Remy, Benoit; Melo de Sousa, Noelita ULg et al

in Proceedings of the 6e Journée de Rencontre Bioforum (2001)

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See detailIdentification of pregnancy-associated glycoprotein 1 (PAG-1) in zebu (Bos indicus) placenta
Melo de Sousa, Noelita ULg; Remy, Benoit; El Amiri, Bouchra et al

in Theriogenology (2001), 55(1), 327

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See detailRadioimmunoassay of pregnancy-associated glycoprotein 1 (PAG1) isolated from zebu (Bos indicus) placenta: preliminary results
Melo de Sousa, Noelita ULg; Sulon, Joseph ULg; Remy, Benoit et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2001), 5(1), 24-25

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See detailIsolation and partial purification of pregnancy-associated glycoproteins from sheep placenta
El Amiri, Bouchra; Remy, Benoit; Sulon, Joseph ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2001), 5(1), 23-24

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See detailPartial Purification of rabbit placental PAG (pregnancy-associated glycoprotein)
Othmani-Mecif, Khira; Benazzoug, Y.; Remy, Benoit et al

in Pflügers Archiv : European Journal of Physiology (2001), 442(R105), 88

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