References of "Remy, B"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailIn vitro oocyte IGF-I priming increases inner cell mass proliferation of in vitro-formed bovine blastocysts
Velazquez, MA; Hadeler, KG; Herrmann, D et al

in Theriogenology (2012), 78(3), 517-527

Studies addressing the effects of supraphysiological levels of IGF-1 on oocyte developmental competence are relevant for unravelling conditions resulting in high bioavailability of IGF-1, such as the ... [more ▼]

Studies addressing the effects of supraphysiological levels of IGF-1 on oocyte developmental competence are relevant for unravelling conditions resulting in high bioavailability of IGF-1, such as the polycystic ovary syndrome (PCOS). This study investigated the effects of supraphysiological levels of IGF-1 during in vivo folliculogenesis on the morula-blastocyst transition in bovine embryos. Compacted morulae were non-surgically collected and frozen for subsequent mRNA expression analysis (IGF1R, IGBP3, TP53, AKT1, SLC2A1, SLC2A3, and SLC2A8), or underwent confocalmicroscopy analysis for protein localization (IGF1R and TP53), or were cultured in vitro for 24 h. In vitro-formed blastocysts were subjected to differential cell staining. The mRNA expression of SLC2A8 was higher in morulae collected from cows treated with IGF-1. Both IGF1R and TP53 protein were present in the plasma membrane and cytoplasm. IGF-1 treatment did not affect protein localization of both IGF1R and TP53. In vitro-formed blastocysts derived from morulae recovered from IGF-1-treated cows displayed a higher number of cells in the inner cell mass (ICM). Total cell number (TCN) of in vitro-formed blastocysts was not affected. A higher mean ICM/TCN proportion was observed in in vitro-formed blastocysts derived from morulae collected from cows treated with IGF-1. The percentage of in vitro-formed blastocysts displaying a low ICM/TCN proportion was decreased by IGF-1 treatment. In vitro-formed blastocysts with a high ICM/TCN proportion were only detected in IGF-1 treated cows. Results show that even a short in vivo exposure of oocytes to a supraphysiological IGF-1 microenvironment can increase ICM cell proliferation in vitro during the morula to blastocyst transition [less ▲]

Detailed reference viewed: 13 (6 ULg)
Full Text
Peer Reviewed
See detailIn vivo oocyte developmental competence is reduced in lean but not in obese superovulated dairy cows after intraovarian administration of IGF1.
Velazquez, M. A.; Hadeler, K. G.; Herrmann, D. et al

in Reproduction (Cambridge, England) (2011), 142(1), 41-52

The present study investigated the role of IGF1 in lactating lean and non-lactating obese dairy cows by injecting 1 μg IGF1 into the ovaries prior to superovulation. This amount of IGF1 has been linked ... [more ▼]

The present study investigated the role of IGF1 in lactating lean and non-lactating obese dairy cows by injecting 1 μg IGF1 into the ovaries prior to superovulation. This amount of IGF1 has been linked with pregnancy loss in women with the polycystic ovary syndrome (PCOS) and was associated with impaired bovine oocyte competence in vitro. Transcript abundance and protein expression of selected genes involved in apoptosis, glucose metabolism, and the IGF system were analyzed. Plasma concentrations of IGF1 and leptin, and IGF1 in uterine luminal fluid (ULF), were also measured. IGF1 treatment decreased embryo viability in lean cows to the levels observed in obese cows. Obese cows were not affected by IGF1 treatment and showed elevated levels of IGF1 (in both plasma and ULF) and leptin. Blastocysts from lean cows treated with IGF1 showed a higher abundance of SLC2A1 and IGFBP3 transcripts. IGF1 treatment reduced protein expression of tumor protein 53 in blastocysts of lean cows, whereas the opposite was observed in obese cows. IGF1 in plasma and ULF was correlated only in the control groups. Blastocyst transcript abundance of IGF1 receptor and IGFBP3 correlated positively with IGF1 concentrations in both plasma and ULF in lean cows. The detrimental microenvironment created by IGF1 injection in lean cows and the lack of effect in obese cows resemble to a certain extent the situation observed in PCOS patients, where IGF1 bioavailability is increased in normal-weight women but reduced in obese women, suggesting that this bovine model could be useful for studying IGF1 involvement in PCOS. [less ▲]

Detailed reference viewed: 13 (2 ULg)
Full Text
Peer Reviewed
See detailSex and PRNP genotype determination in preimplantation caprine embryos
Guignot, F.; Perreau, C.; Cavarroc, C. et al

in Reproduction in Domestic Animals (2011), 46(4), 656-663

The objective of this study was to test the accuracy of genotype diagnosis after whole amplification of DNA extracted from biopsies obtained by trimming goat embryos and to evaluate the viability of ... [more ▼]

The objective of this study was to test the accuracy of genotype diagnosis after whole amplification of DNA extracted from biopsies obtained by trimming goat embryos and to evaluate the viability of biopsied embryos after vitrification/warming and transfer. Whole genome amplification (WGA) was performed using Multiple Displacement Amplification (MDA). Sex and prion protein (PRNP) genotypes were determined. Sex diagnosis was carried out by PCR amplification of ZFX/ZFY and Y chromosome-specific sequences. Prion protein genotype determination was performed on codons 142, 154, 211, 222 and 240. Embryos were collected at day 7 after oestrus and biopsied either immediately after collection (blastocysts and expanded blastocysts) or after 24 h of in vitro culture (compacted morulae). Biopsied embryos were frozen by vitrification. Vitrified whole embryos were kept as control. DNA of biopsies was extracted and amplified using MDA. Sex diagnosis was efficient for 97.4% of biopsies and PRNP genotyping was determined in 78.7% of biopsies. After embryo transfer, no significant difference was observed in kidding rate between biopsied and vitrified control embryos, whereas embryo survival rate was different between biopsied and whole vitrified embryos (p = 0.032). At birth, 100% of diagnosed sex and 98.2% of predetermined codons were correct. Offspring PRNP profiles were in agreement with parental genotype. Whole genome amplification with MDA kit coupled with sex diagnosis and PRNP genotype predetermination are very accurate techniques to genotype goat embryos before transfer. These novel results allow us to plan selection of scrapie-resistant genotypes and kid sex before transfer of cryopreserved embryo. [less ▲]

Detailed reference viewed: 42 (3 ULg)
Full Text
Peer Reviewed
See detailEfficiency of day seven collection of bovine embryosafter superovulation by flushing the oviducts and the uterin horns.
Havlicek, V.; Kuzmany, A.; Beckers, Jean-François ULg et al

in Reproduction, Fertility and Development (2010), 22(1), 363

Detailed reference viewed: 15 (7 ULg)
Full Text
See detailTransferêcia de embrioes como ferramenta para formaçao de rebanho experimental ovino
Rizzo, Huber; François, Dominique; Fassier, T et al

in Ciência Animal Brasileira (2009), Suppl 1

Detailed reference viewed: 7 (0 ULg)
Full Text
See detailEmbryo transfer as a tool for experimental reproduction of ovine herds.
Rizzo, H.; François, D.; Fassier, T. et al

in Ciência Animal Brasileira (2009), Suppl 1

Detailed reference viewed: 11 (3 ULg)
Full Text
Peer Reviewed
See detailEffects of intraovarian application of insulin-like growth factor-1 (IGF-1) on the superovulatory response of diary cattle.
Velazquez, M. A.; Hadeler, K. G.; Beckers, Jean-François ULg et al

in Reproduction in Domestic Animals (2009), 44(S1), 38

Detailed reference viewed: 20 (2 ULg)
Full Text
Peer Reviewed
See detailBiochemical markers of bone metabolism in Ardennes horses
Pastoret, V.; Carstanjen, V.; Hoyle, N. R. et al

Poster (2006, January)

Detailed reference viewed: 3 (0 ULg)
Full Text
Peer Reviewed
See detailBovine pepsinogen A: Isolation and parial characterization of isoforms with high activity
Idrissa-Sidikou, D.; Remy, B.; Gerardin-Otthiers, Nicole ULg et al

in Journal of Animal and Veterinary Advances (2005)

The goal of this study was to purify bovine pepsinogen by a simple method allowing the preparation of large amount of pure protein. The purified protein and antisera are needed to develop diagnostic ... [more ▼]

The goal of this study was to purify bovine pepsinogen by a simple method allowing the preparation of large amount of pure protein. The purified protein and antisera are needed to develop diagnostic methods for further investigations in animals susceptible of gastric disorders or helminthosis. Pepsinogen isoforms were separated from extracts of bovine fundic mucosa by ammonium sulfate precipitations and chromatography on DEAE and hydroxyapatite. The isoforms showed a high activity in indirect proteolytic assay. Sequence analysis gave the following amino acid sequence SVVKIPLVKK for fraction 1, 2 and SVVKIPLVKKKSLRQNLIENGKLKE for fraction 3. The Mass spectrometry revealed isoforms with different masses from 39,864 to 40,181 Da. The estimated organic phosphate content ranged from 0.98 to 3.9 moles of phosphate per molecule. The protocol, with few steps, gave consequent quantities of pure and active protein available for further studies including the development of RIA and ELISA as diagnostic tools in gastrointestinal diseases. [less ▲]

Detailed reference viewed: 16 (5 ULg)
Full Text
Peer Reviewed
See detailDevelopment of a Radioimmunoassay for Bovine Pepsinogen A
Sidikou, I. D.; Remy, B.; Beckers, Jean-François ULg

in Revue d'Elévage et de Médecine Vétérinaire des Pays Tropicaux (2005), 58(4), 229-235

Pepsinogen A is the most abundant zymogen found in blood, and its enzymatic measurement is used for the diagnosis of gastric lesions. The present study was conducted to develop a radioimmunoassay (RIA ... [more ▼]

Pepsinogen A is the most abundant zymogen found in blood, and its enzymatic measurement is used for the diagnosis of gastric lesions. The present study was conducted to develop a radioimmunoassay (RIA) specific to pepsinogen A in bovine plasma. The authors purified large amounts of three non-denatured isoforms of bovine pepsinogen A with high proteolytic activity. These homogeneous preparations were used to produce specific antisera in New Zealand White rabbits, and three antisera with high titers were obtained. In the present assay the antiserum #822 was used at a final dilution of 1/250,000. The detection limit of the assay was 20 ng/ml and the recovery ranged from 85.5 to 103.3%. The repeatability (intra-assay coefficient of variation) was lower than 6.6%, whereas the reproducibility (inter-assay coefficient of variation) was lower than 13.4%. The capacity of the RIA to detect pepsinogen A in blood was tested by measuring the concentrations in plasma of newborn calves (n = 6) serially sampled from birth to four months of age. The mean pepsinogen A value (mean ± standard deviation) in the plasma of calves was 2071 ± 752 ng/ml one day after birth. The concentration decreased progressively and was about 1196 ± 307 ng/ml at day 21, and 677 ± 109 ng/ml at day 120. The present study is the first report on pepsinogen A concentrations in bovine measured by RIA. Further investigations using the RIA should be performed in order to confirm these values and determine pepsinogen levels in older cattle in physiological and pathological conditions such as gastrointestinal helminthosis [less ▲]

Detailed reference viewed: 13 (1 ULg)
Full Text
Peer Reviewed
See detailBovine pepsinogen and Prochymosin : Current knowledge, applications and outlines in the management of gastrointestinal worms
Sidikou, I. D.; Remy, B.; Hornick, Jean-Luc ULg et al

in Annales de Médecine Vétérinaire (2005), 149(4), 213-228

The characterization of the gastric aspartic proteases and the understanding of their mechanisms of activation led to several applications in many domains as the use of chymosin in cheese industries and ... [more ▼]

The characterization of the gastric aspartic proteases and the understanding of their mechanisms of activation led to several applications in many domains as the use of chymosin in cheese industries and the diagnostic of gastric diseases in humans and animals. Particularly, in cattle, the indirect measurement of pepsinogen activity is largely used for the diagnosis of ostertagiosis. Today, the gastric strongyloses are responsible for serious problems of management or the emergence of parasites resistence towards antihelminthics, and no immediate solution is available. Nevertheless, several tracks are proposed such as a more rational use of antihelminthics by the study of blood pepsinogen. This approach held our attention. The present review describes bovine pepsinogen and prochymosin, with an accent on the zymogen structure, their mechanisms of activation and the methods of blood levels measurement. The manuscript will end on the importance of the problems raised by gastric worms, and on the interest of the measurement of blood pepsinogen in the management of these diseases in cattle. [less ▲]

Detailed reference viewed: 54 (3 ULg)
Peer Reviewed
See detailAnalytical, physiological and clinical validation of a radioimmunoassay for serum Procollagen Type-III aminoterminal peptide in the dog
Schuller, S.; Valentin, S.; Remy, B. et al

in 14th ESVIM Meeting - Barcelona - Espagne (2004, June)

Detailed reference viewed: 3 (0 ULg)
Full Text
Peer Reviewed
See detailEffet de prétraitements agoniste et antagoniste de GnRH sur la production d’embryons chez la brebis et la chèvre
Baril, G.; Cognié, Y.; Belloc, J. P. et al

in Proceedings: 11e Rencontres autour des Recherches sur les Ruminants (3R) (2004)

Detailed reference viewed: 15 (2 ULg)
See detailInsémination artificielle et transfert embryonnaire pour limiter la dissémination des maladies contagieuses chez les animaux de rente.
Gonzalez, F; Cabrera, F; Rodriguez, N et al

Conference (2003, November)

Detailed reference viewed: 10 (0 ULg)
Peer Reviewed
See detailThe effect of race training on biochemical bone markers in young thoroughbreds
Carstanjen, B; Lepage, OM; Sulon, J et al

in Proceedings of the 13th annual scientific meeting of the ECVS (2003)

Detailed reference viewed: 3 (0 ULg)
Full Text
Peer Reviewed
See detailPregnancy-Associated Glycoprotein 55h [Fragment] from Ovis aries placenta - Access number P83500
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

E-print/Working paper (2003)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

Detailed reference viewed: 6 (0 ULg)
Full Text
Peer Reviewed
See detailPregnancy-Associated Glycoprotein 59g [Fragment] from Ovis aries placenta - Access number P83499
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

E-print/Working paper (2003)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

Detailed reference viewed: 3 (0 ULg)
Full Text
Peer Reviewed
See detailPregnancy-Associated Glycoprotein 60f [Fragment] from Ovis aries placenta - Access number P83498
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

E-print/Working paper (2003)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

Detailed reference viewed: 4 (0 ULg)
Full Text
Peer Reviewed
See detailPregnancy-Associated Glycoprotein 66e [Fragment] from Ovis aries placenta - Access number P83497
El Amiri, B.; Remy, B.; Melo de Sousa, Noelita ULg et al

E-print/Working paper (2003)

N-terminal microsequence obtained after purification and characterization of placental proteins in ovine species. Proteins were submitted to SwissProt databank.

Detailed reference viewed: 4 (0 ULg)