 Early pregnancy detection in sheep. Secondary communication; Beckers, Jean-François  ; Sulon, Joseph et alPoster (2005) A szerzők a juhok vemhességének korai megállapítására összehasonlították a progeszteron- (P4) és a vemhességi fehérje (PAG) RIA-tesztek megbízhatóságát. A PAG-teszttel a nem vemhes állatok nagyobb ... [more ▼] A szerzők a juhok vemhességének korai megállapítására összehasonlították a progeszteron- (P4) és a vemhességi fehérje (PAG) RIA-tesztek megbízhatóságát. A PAG-teszttel a nem vemhes állatok nagyobb biztonsággal voltak felismerhetők. Nagyszámú állaton (n=949 és 764) végzett rectalis ultrahangvizsgálattal a vemhességet pontosabban tudták megállapítani, ha az állatokat 12 óráig koplaltatták és vizsgálat közben a hasfalat a tőgy előtt felemelték. Az ultrahangvizsgálat és a PAG-teszt eredményeinek összehasonlításával megállapították, hogy az utóbbi megbízhatóbb eredményt ad. [less ▲] Detailed reference viewed: 4 (0 ULg) Effect of the type and number of prostaglandin treatments on corpus luteum, the largest follicle and progesterone concentration in dairy cows; Beckers, Jean-François ; Sulon, Joseph et alin Reproduction in Domestic Animals (2005), 40(5), 436-442 Lactating dairy cows (n = 72) with a mature corpus luteum (CL) (diameter of >= 17 mm) determined by ultrasonography and having a follicle with a diameter of >= 10 min were randomly assigned to four groups ... [more ▼] Lactating dairy cows (n = 72) with a mature corpus luteum (CL) (diameter of >= 17 mm) determined by ultrasonography and having a follicle with a diameter of >= 10 min were randomly assigned to four groups. Cows were treated with cloprostenol i.m. once or twice, or with dinoprost i.m. once or twice with an 8-h interval. The ovaries of each cow were scanned daily by transrectal ultrasonography to measure the changes in the areas of CL and the largest follicle and to determine the occurrence of ovulation. Oestrus was verified twice daily. In addition, blood sample was withdrawn from each cow daily for measuring progesterone (P4) concentrations. Significant decreases in the percentage changes relative to areas of CL and P4 concentrations or increases in the percentage changes in the area of the largest follicle on day 0 were detected in each group during the experiment. However, the type of the drug and the number of the treatments had no significant effect on those parameters. Cows ovulated with or without showing oestrus (group A) and cows exhibiting no oestrus and ovulation (group 13) were also evaluated. In contrast to the mean area of the CL and the mean concentration of P4 on day 0, the mean area of the largest follicles between, the two groups on day 0 differed significantly Significant decreases in the percentage changes relative to the area of the CL and P4 concentration or increases in the percentage changes relative to the area of the largest follicle during the experiment were detected in both groups; however, there were no group differences. Treatment of dairy cows with two injections of prostaglandins (cloprostenol or dinoprost) at an 8-h interval resulted in more cows being, observed in oestrus within 5 days after treatment and having significantly higher pregnancy rate than those treated with a single prostaglandin injection. [less ▲] Detailed reference viewed: 22 (1 ULg)Accuracy of transrectal ultrasonography for determination of pregnancy in sheep: effect of fasting and handling of the animals; ; et al in Theriogenology (2004), 61(7-8), 1291-1298 The present study was performed to investigate the effect of previous fasting and lifting of the abdomen of the ewes during transrectal ultrasonographic scanning on the results of early pregnancy ... [more ▼] The present study was performed to investigate the effect of previous fasting and lifting of the abdomen of the ewes during transrectal ultrasonographic scanning on the results of early pregnancy diagnosis. Ewes of four flocks (A, B, C and D; all Awassi x Merino ewes, n = 1247) aged 0.7-10 years were used in this study. These ewes were estrus synchronized and artificially inseminated. From 2 weeks later onwards, fertile rams were kept with the ewes of flocks A, B and C (n = 949) for natural breeding, while ewes of flock D (n = 298) were re-inseminated 17 days later. Transrectal ultrasonography (5 MHz) was carried out in ewes of flocks A, B and C on four separate occasions but only once in ewes of flock D. For final analysis, animals were divided over two groups: ewes of Group 1 (n = 949 scans) were scanned in a standing position within the milking parlor. Animals of Group 2 (n = 764 scans) were scanned by the same operator and with the same scanning technique, but these ewes were fasted for 12 h prior to scanning and the abdominal wall was lifted, just in front of the udder during scanning. The sensitivity of the test for diagnosing pregnancy at Days 18-24, 25-30, 31-40 and 41-50 was 21.8, 32.3, 63.3 and 50% in Group 1, and 46, 92.5, 92.3 and 96.8% in Group 2, respectively. Only within Group 1, the sensitivity of the test was higher in young ewes (0.7-2 years) than in older ones (>2-10 years). Significant differences were observed at scan periods Days 18-24 and Days 4150 of gestation. It is concluded that, fasting prior to scanning and lifting the abdomen during scanning significantly improve the accuracy of transrectal ultrasonographic pregnancy diagnosis in Awassi x Merino ewes. [less ▲] Detailed reference viewed: 24 (0 ULg)Evaluation of false transrectal ultrasonographic pregnancy diagnoses in sheep by measuring the plasma level of pregnancy-associated glycoproteins; Beckers, Jean-François ; Sulon, Joseph et alin Reproduction Nutrition Development (2003), 43(6, NOV-DEC), 577-586 The present study was undertaken to investigate to what extent pregnancy diagnoses made by transrectal ultrasonography could be confirmed by measurements of plasma concentration of ovine pregnancy ... [more ▼] The present study was undertaken to investigate to what extent pregnancy diagnoses made by transrectal ultrasonography could be confirmed by measurements of plasma concentration of ovine pregnancy-associated glycoproteins (ovPAG). A total of 424 Awassi x Merino ewes were synchronized for estrus and examined by transrectal ultrasonography. In Experiment 1, the ewes (n = 156) were repeatedly scanned in a standing position on d 29, 36 and 50 of gestation. Similarly, the ewes (n = 268) in Experiment 2 were scanned on d 24, 29 and 34 of gestation, but these ewes were fasted for 12 h prior to the examination and the abdominal wall of each animal was lifted up by the hands of the assistant during the scanning. Blood samples were withdrawn after each transrectal ultrasonographic examination in both experiments. Ovine PAG concentrations were measured in plasma by a heterologous radioimmunoassay and the cut-off value for pregnancy was greater than or equal to 1 ng.mL(-1). Based on the lambing performance, in Experiment 1, altogether 47 false negative and 38 false positive diagnoses were made by transrectal ultrasonography in 24 and 33 ewes, respectively between d 29 and 50 of gestation. In Experiment 2, altogether 8 false negative and 13 false positive diagnoses both were made in 7 ewes between d 24 and 34 of gestation. In both experiments, all ewes with false negative diagnoses had ovPAG concentrations higher than the threshold level for pregnancy diagnosis and all ewes with false positive diagnoses had ovPAG concentrations lower than the threshold of pregnancy. Furthermore, by the PAG-RIA test all lambed or aborted ewes (n = 63) were correctly diagnosed as pregnant and with three exceptions, all non-lambed ewes (n = 361) were correctly diagnosed as non-pregnant during the examined periods of both experiments. [less ▲] Detailed reference viewed: 44 (0 ULg)Effect of different doses of prostaglandin on the area of corpus luteum, the largest follicle and progesterone concentration in the dairy cow; Beckers, Jean-François ; Sulon, Joseph et alin Reproduction in Domestic Animals (2003), 38(6), 423-428 Lactating dairy cows with a mature corpus luteum (CL) (diameter of greater than or equal to17 mm determined by ultrasonography) and having a follicle with a diameter of 10 mm (n = 49) were randomly ... [more ▼] Lactating dairy cows with a mature corpus luteum (CL) (diameter of greater than or equal to17 mm determined by ultrasonography) and having a follicle with a diameter of 10 mm (n = 49) were randomly assigned to three groups. The first group was treated with a single dose of exogenous prostaglandin (25 mg), while the second group was treated with 35 mg on day 0, and the third group served as control in order to evaluate the effect of rectal manipulation on the CL during ultrasonographic examination. Blood samples were collected daily for analysing progesterone (P4) concentrations. In group 1 the incidence of oestrus and artificial insemination (AI) in 10 days after treatment was 95% (19 of 20). The conception rate was 31.6%, and the average time to oestrus after treatment was 3.7 day. In group 2 the incidence of oestrus and A.I. was 84.2% (16/19). The conception rate was 31.2%, and the average time to oestrus after treatment was 2.8 day. In the untreated group only two cows (2/10) exhibited oestrus during the examined period and none of them became pregnant. There were no significant differences between the two treated groups in terms of reduction in the area of CL and P4 concentrations and of an increase in the area of the dominant follicles. At the same time, the decrease in the percentage changes relative to the area of CL and to the concentrations of P4 were statistically significant in both treated groups. [less ▲] Detailed reference viewed: 10 (2 ULg)[Külonböző dózisú prosztagandin készítmények hatása a sárgatest luteolízisére és a vemheslésre; Beckers, Jean-François ; Sulon, Joseph et alin Proceedings of the 12th Magyar Buiatrikus Kongresszus (2001) Detailed reference viewed: 23 (2 ULg) |
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