References of "Pesesse, Laurence"
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See detailBone sialoprotein as a potential key factor implicated in the pathophysiology of osteoarthritis
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Walsh, David et al

in Osteoarthritis and Cartilage (2014), 22(4), 547-56

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See detailASSOCIATION BETWEEN CHONDROCYTE HYPERTROPHY AND ANGIOGENESIS OF CARTILAGE IN OSTEOARTHRITIS
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Delcour, Jean-Pierre et al

Conference (2013, November)

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See detailBONE SIALOPROTEIN AS A KEY FACTOR IN THE PATHOPHYSIOLOGY OF OSTEOARTHRITIS
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Walsh, David et al

Conference (2013, November)

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See detailConsequences of chondrocyte hypertrophy on osteoarthritic cartilage: potential effect on angiogenesis.
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Delcour, J.-P. et al

in Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society (2013), 21(12), 1913-23

OBJECTIVE: The aim of this study was to investigate the link between the hypertrophic phenotype of chondrocytes and angiogenesis in osteoarthritis (OA) and more particularly to demonstrate that OA ... [more ▼]

OBJECTIVE: The aim of this study was to investigate the link between the hypertrophic phenotype of chondrocytes and angiogenesis in osteoarthritis (OA) and more particularly to demonstrate that OA hypertrophic chondrocytes potentially express a phenotype promoting angiogenesis through the expression of factors controlling endothelial cells migration, invasion and adhesion. METHOD: Human OA chondrocytes were cultivated in alginate beads in medium supplemented with 10% fetal bovine serum (FBS) to induce chondrocyte hypertrophy. The hypertrophic phenotype was characterized throughout 28 days of culture by measuring the expression of specific genes and by a microscopic observation of cellular morphology. The effect of media conditioned by OA hypertrophic chondrocyte on endothelial cells migration, invasion and adhesion was evaluated in functional assays. Moreover, hypertrophic OA chondrocytes were tested for the expression of angiogenic factors by real-time RT-PCR. RESULTS: Specific markers of hypertrophy and observation of cellular morphology attested of the hypertrophic phenotype of chondrocytes in our culture model. Functional angiogenesis assays showed that factors produced by hypertrophic chondrocytes stimulated migration, invasion and adhesion of endothelial cells. Among the evaluated angiogenic factors, bone sialoprotein (BSP) was the most highly upregulated in hypertrophic chondrocytes. The inhibition of endothelial cell adhesion by a GRGDS peptide confirmed the implication of RGD domain proteins, like BSP, in hypertrophic chondrocyte-induced adhesion of endothelial cells. CONCLUSION: Hypertrophic differentiation of chondrocyte may promote angiogenesis. Our findings established the relation of BSP with OA chondrocyte hypertrophy and suggested that this factor could constitute a potential target to control cartilage neovascularisation in OA. [less ▲]

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See detailLa bone sialoproteine: un facteur clé dans la pathogénie de l'arthrose
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Delcour, Jean-Pierre et al

Conference (2012, December)

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See detailAssociation entre l'hypertrophie du chondrocyte et l'angiogenèse du cartilage dans l'arthrose
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Delcour, Jean-Pierre et al

Conference (2012, December)

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See detailSubchondral bone and osteoarthritis: biological and cellular aspects
Henrotin, Yves ULg; Pesesse, Laurence ULg; Sanchez, Christelle ULg

in Osteoporosis International (2012), 23(Suppl 8), 847851

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See detailAssociation between chondrocytes hypertrophy and angiogenesis of cartilage in osteoarthritis
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Delcour, Jean-Pierre et al

Poster (2012, November 13)

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See detailAssociation between chondrocytes hypertrophy and angiogenesis of cartilage in osteoarthritis
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Delcour, Jean-Pierre et al

in Arthritis and Rheumatism (2012, October), 64(10 (Suppl)), 760

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See detailLa bone sialoproteine: un facteur clé dans la pathogénie de l'arthrose
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Delcour, Jean-Pierre et al

in Revue du Rhumatisme (2012), 79(S), 106

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See detailBone sialoprotein: a key mediator of the angiogenic activity of hypertrophic osteoarthritic chondrocytes
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Baudouin, Caroline et al

in Osteoarthritis and Cartilage (2012), 20(S), 122-123

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See detailAssociation entre l'hypertrophie du chondrocyte et l'angiogenèse du cartilage dans l'arthrose
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Delcour, Jean-Pierre et al

in Revue du Rhumatisme (2012), 79(S), 105-106

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See detailRegulation of subchondral bone osteoblast metabolism by cyclic compression.
Sanchez, Christelle ULg; Pesesse, Laurence ULg; Gabay, Odile et al

in Arthritis and Rheumatism (2012), 64(4), 1193-203

OBJECTIVE: Recent data have shown that abnormal subchondral bone remodeling plays an important role in osteoarthritis (OA) onset and progression, and it was suggested that abnormal mechanical pressure ... [more ▼]

OBJECTIVE: Recent data have shown that abnormal subchondral bone remodeling plays an important role in osteoarthritis (OA) onset and progression, and it was suggested that abnormal mechanical pressure applied to the articulation was responsible for these metabolic changes. This study was undertaken to evaluate the effects of cyclic compression on osteoblasts from OA subchondral bone. METHODS: Osteoblasts were isolated from sclerotic and nonsclerotic areas of human OA subchondral bone. After 28 days, the osteoblasts were surrounded by an abundant extracellular matrix and formed a resistant membrane, which was submitted to cyclic compression (1 MPa at 1 Hz) for 4 hours. Gene expression was evaluated by reverse transcription-polymerase chain reaction. Protein production in culture supernatants was quantified by enzyme-linked immunosorbent assay or visualized by immunohistochemistry. RESULTS: Compression increased the expression of genes coding for interleukin-6 (IL-6), cyclooxygenase 2, RANKL, fibroblast growth factor 2, IL-8, matrix metalloproteinase 3 (MMP-3), MMP-9, and MMP-13 but reduced the expression of osteoprotegerin in osteoblasts in both sclerotic and nonsclerotic areas. Colalpha1(I) and MMP-2 were not significantly affected by mechanical stimuli. Nonsclerotic osteoblasts were significantly more sensitive to compression than sclerotic ones, but after compression, differences in messenger RNA levels between nonsclerotic and sclerotic osteoblasts were largely reduced or even abolished. Under basal conditions, sclerotic osteoblasts expressed similar levels of alpha5, alphav, beta1, and beta3 integrins and CD44 as nonsclerotic osteoblasts but 30% less connexin 43, an important mechanoreceptor. CONCLUSION: Genes involved in subchondral bone sclerosis are mechanosensitive. After compression, nonsclerotic and sclerotic osteoblasts expressed a similar phenotype, suggesting that compression could be responsible for the phenotype changes in OA subchondral osteoblasts. [less ▲]

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See detailChapiter 10: Identification of mechanosensitive genes in chondrocytes and osteoblasts and their role in OA pathogenesis
Henrotin, Yves ULg; Pesesse, Laurence ULg; Sanchez, Christelle ULg

in Kamklin, Andre; Kiseleva, Irina (Eds.) Mechanical stretch and cytokines (2012)

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