References of "Oswald, E"
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See detailDiagnostic strategy for identifying avian pathogenic Escherichia coli based on four patterns of virulence genes
Schouler, C; Schaeffer, B; Brée, A et al

in Journal of Clinical Microbiology (2012), 50(5), 1673-1678

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See detailCommon virulence factors and genetic relationships between O18 : K1 : H7 Escherichia coli isolates of human and avian origin
Moulin-Schouleur, M.; Schouler, C.; Tailliez, P. et al

in Journal of Clinical Microbiology (2006), 44(10), 3484-3492

Extraintestinal pathogenic (ExPEC) Escherichia coli strains of serotype 018:K1:H7 are mainly responsible for neonatal meningitis and sepsis in humans and belong to a limited number of closely related ... [more ▼]

Extraintestinal pathogenic (ExPEC) Escherichia coli strains of serotype 018:K1:H7 are mainly responsible for neonatal meningitis and sepsis in humans and belong to a limited number of closely related clones. The same serotype is also frequently isolated from the extraintestinal lesions of colibacillosis in poultry, but it is not well known to what extent human and avian strains of this particular serotype are related. Twenty-two ExPEC isolates of human origin and 33 isolates of avian origin were compared on the basis of their virulence determinants, lethality for chicks, pulsed-field gel electrophoresis (PFGE) patterns, and classification in the main phylogenetic groups. Both avian and human isolates were lethal for chicks and harbored similar virulence genotypes. A major virulence pattern, identified in 75% of the isolates, was characterized by the presence of F1 variant fimbriae; S fimbriae; IbeA; the aerobactin system; and genomic fragments A9, A12, D1, D7, D10, and D11 and by the absence of P fimbriae, F1C fimbriae, Afa adhesin, and CNF1. All but one of the avian and human isolates also belonged to major phylogenetic group B2. However, various subclonal populations could be distinguished by PFGE in relation to animal species and geographical origin. These results demonstrate that very closely related clones can be recovered from extraintestinal infections in humans and chickens and suggest that avian pathogenic E. coli isolates of serotype 018:K1:H7 are potential human pathogens. [less ▲]

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See detailSerotypes and intimin types of intestinal and faecal strains of eae+ Escherichia coli from weaned pigs
Malik, A.; Toth, I.; Beutin, L. et al

in Veterinary Microbiology (2006), 114

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See detailEnteropathogenic and enterohaemorrhagic Escherichia coli deliver a novel effector called Cif, which blocks cell cycle G(2)/M transition
Marches, O.; Ledger, T. N.; Boury, M. et al

in Molecular Microbiology (2003), 50(5), 1553-1567

Enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E. coli (EHEC) are closely related pathogens. Both use a type III secretion system (TTSS) encoded by the 'locus of enterocyte effacement ... [more ▼]

Enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E. coli (EHEC) are closely related pathogens. Both use a type III secretion system (TTSS) encoded by the 'locus of enterocyte effacement' (LEE) to subvert and attach to epithelial cells through the injection of a repertoire of effector molecules. Here, we report the identification of a new TTSS translocated effector molecule called Cif, which blocks cell cycle G(2)/M transition and induces the formation of stress fibres through the recruitment of focal adhesions. Cif is not encoded by the LEE but by a lambdoid prophage present in EPEC and EHEC. A cif mutant causes localized effacement of microvilli and intimately attaches to the host cell surface, but is defective in the ability to block mitosis. When expressed in TTSS competent LEE-positive pathogens, Cif is injected into the infected epithelial cells. These cells arrested at the G(2)/M phase displayed accumulation of inactive phosphorylated Cdk1. In conclusion, Cif is a new member of a growing family of bacterial cyclomodulins that subvert the host eukaryotic cell cycle. [less ▲]

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See detailPutative roles of the CNF2 and CDTIII toxins in experimental infections with necrotoxigenic Escherichia coli type 2 (NTEC2) strains in calves
Van Bost, S.; Roels, S.; Oswald, E. et al

in Microbes & Infection (2003), 5(13), 1189-1193

Newborn colostrum-restricted calves were orally inoculated with an Escherichia coli strain, identified originally as non-pathogenic, and into which the plasmid pVir was conjugally transferred. This ... [more ▼]

Newborn colostrum-restricted calves were orally inoculated with an Escherichia coli strain, identified originally as non-pathogenic, and into which the plasmid pVir was conjugally transferred. This resulted in diarrhea, intestinal lesions and extra-intestinal invasion, suggesting that factors affecting these pathogenic properties are located on pVir. In order to analyze the respective roles of the toxins CNF2 and CDTIII in the pathogenesis, colostrum-restricted calves were inoculated with isogenic mutants in the cnf2 and the cdt-III genes. The loss of cnf2 is associated with a reduction in the pathogenicity, since diarrhea does not occur in calves challenged, in spite of successful colonization of the intestine. Nevertheless, the mutant strain remains able to invade the bloodstream and to localize in the internal organs. Conversely, the calves inoculated with mutant in the cdt-III gene evolved in the same way as wild-type strain-inoculated calves with regard to clinical signs and macroscopic or microscopic lesions. (C) 2003 Editions scientifiques et medicales Elsevier SAS. All rights reserved. [less ▲]

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See detailMultiplex PCRs for identification of necrotoxigenic Escherichia coli
Van Bost, S.; Jacquemin, E.; Oswald, E. et al

in Journal of Clinical Microbiology (2003), 41(9), 4480-4482

Two multiplex PCRs were developed for the detection of necrotoxigenic Escherichia coli virulence genes. M1 contained the primers for the toxins and the aerobactin, and M2 contained the primers for the ... [more ▼]

Two multiplex PCRs were developed for the detection of necrotoxigenic Escherichia coli virulence genes. M1 contained the primers for the toxins and the aerobactin, and M2 contained the primers for the adhesins. They were validated by single PCRs performed with reference E. coli strains and by multiplex PCRs with necrotoxigenic E. coli strains isolated from different animal species. [less ▲]

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See detailPrevalence and identity of cdt-related sequences in necrotoxigenic Escherichia coli
Mainil, Jacques ULg; Jacquemin, E.; Oswald, E.

in Veterinary Microbiology (2003), 94

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See detailExamination of Escherichia coli from poultry for selected adhesin genes important in disease caused by mammalian pathogenic E. coli
Stordeur, Philippe; Marlier, Didier ULg; Blanco, J. et al

in Veterinary Microbiology (2002), 84(3), 231-241

A collection of 1601 extraintestinal and intestinal Escherichia coli isolated from chickens, turkeys and ducks. in Belgium, France and Spain, was hybridised with gene probes specific for fimbrial and ... [more ▼]

A collection of 1601 extraintestinal and intestinal Escherichia coli isolated from chickens, turkeys and ducks. in Belgium, France and Spain, was hybridised with gene probes specific for fimbrial and afimbrial adhesins (F17, F18, S (Sfa/F1C), Bfp, Afa, Cs31A, Intimin (Eae), Aida-1) of intestinal, urinary and invasive E. coli of mammals and with a probe specific for the P (Pap/Prs) fimbrial adhesin of urinary and invasive E. coli of mammals and birds. Three hundred and eighty-three strains (23.9%) were P-positive, 76 strains (4.8%) were Afa-positive, 75 strains (4.7%) were F17-positive, 67 strains (4.2%) were S-positive, 23 (1.4%) were Intimin-positive. and all were F18-, Cs31A-, Aidal- and Bfp-negative. The 75 F17-positive strains harboured different major subunit A-encoding gene variants, but the f17Ac variant was the most frequent (52 strains, 69,3%) and seven strains (9.3%) were not typeable. The f17G gene variant coding for the GII adhesin was the most frequent (56 strains, 75.0%), whereas the f17GI gene variant was present in four strains (5%) and 15 strains (20.0%) were not typeable. All Afa-positive strains harboured the afa-8 variant. The 23 Intimin-positive E. coli tested positive for the beta -variant (16 strains; 69.6%) or for the gamma -variant (seven strains;, 30.4%) of the eae gene. Chicken and turkey E. coli were more frequently probe-positive (43.6 and 43.1 %, respectively) than duck E. coli 31.5%) and extraintestinal E. coli were also more frequently probe-positive (48.4%) than intestinal strains (18.5%). Different combinations of probe positive hybridisation results were observed in 72 of the 540 probe-positive E. coli (13.3%). The most frequent combinations were between AfaE-8 and F17 probes (47 strains; 8.7%) and between P and S probes (13 strains. 2.4%). Although f17- and afa-8-related DNA sequences can be plasmid-located in mammalian E. coli. they were not in avian E. coli. Besides the P fimbrial adhesin, F17 and S fimbrial and Afa-VIII and Intimin afimbrial adhesins may thus represent colonisation factors of avian pathogenic E. coli. (C) 2002 Elsevier Science B.V. All rights reserved. [less ▲]

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See detailNekrotoxikus Escherichia coli (NTEC) törzsek magyarorszagi elöfordulasa és jellembzése
Nagy, B.; Toth, I.; Mainil, Jacques ULg et al

in Magyar Allatorvosok Lapja = Hungarian Veterinary Journal (2000), 122

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See detailCharacterization of intestinal cnf1+ Escherichia coli from weaned piglets
Toth, I.; Oswald, E.; Mainil, Jacques ULg et al

in International Journal of Medical Microbiology (2000), 290

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See detailRole of Tir and Intimin in the virulence of rabbit enteropathogenic Escherichia coli (REPEC) of serotype O103:H2
Marchès, O.; Nougayrede, J. P.; Boullier, S. et al

in Infection and Immunity (2000), 68

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See detailComparison of necrotoxigenic Escherichia coli isolates from farm animals and humans
Mainil, Jacques ULg; Jacquemin, E.; Pohl, P. et al

in Veterinary Microbiology (1999), 70

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See detailThe long-term cytoskeletal rearrangement induced by rabbit enteropathogenic Escherichia coli is Esp-dependent but intimin-independent
Nougayrède, J. P.; Marchès, O.; Boury, M. et al

in Molecular Microbiology (1999), 31

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See detailPresence of pep-, sfa- and afa-related sequences in necrotoxigenic Escherichia coli isolates from cattle : evidence for new variants of the AFA family
Mainil, Jacques ULg; Jacquemin, E.; Hérault, F. et al

in Canadian Journal of Veterinary Research = Revue Canadienne de Recherche Vétérinaire (1997), 61

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See detailTwo sensitive and specific DNA probes to detect Escherichia coli strains encoding Cytotoxic Necrotizing Factor 1 or 2
Oswald, E.; Pohl, P.; Jacquemin, E. et al

in Journal of Medical Microbiology (1994), 40

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See detailEscherichia coli producing CNF1 and CNF2 cytotoxins in animals with different disorders
Pohl, P.; Oswald, E.; Van Muylem, K. et al

in Veterinary Research (1993), 24

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See detailTests simples pour le diagnostic présumé des Escherichia coli isolées principalement chez les bovins et productrices des cytotoxines nécrosantes (CNF)
Pohl, P.; Oswald, E.; Van Robaeys, G. et al

in Annales de Médecine Vétérinaire (1993), 137

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See detailEscherichia coli productrices de la toxine cytotoxique nécrosante de type 1 (CNF1) isolées à partir de processus pathologiques chez des chats et des chiens
Pohl, P.; Mainil, Jacques ULg; Devriese, L. et al

in Annales de Médecine Vétérinaire (1993), 137

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See detailFacteurs de virulence et phénotypes de soixante et une souches d'Escherichia coli d'origine bovine, productrices de la toxine cytotoxique nécrosante de type 1 (CNF 1).
Pohl, P.; Daube, Georges ULg; Mainil, Jacques ULg et al

in Annales de Recherches Vétérinaires = Annals of Veterinary Research (1992), 23(1), 83-91

Virulence factors and phenotypes of 61 strains CNF1+ were investigated. Eighty-nine percent of the strains produced an aerobactin and were resistant to the bactericidal activity of sheep serum, both of ... [more ▼]

Virulence factors and phenotypes of 61 strains CNF1+ were investigated. Eighty-nine percent of the strains produced an aerobactin and were resistant to the bactericidal activity of sheep serum, both of which are properties of septicemic strains of E coli. None of the strains reacted either with DNA probes corresponding to the enterotoxins STaP, STb, LT-I and LT-IIa, or to the verotoxins VT-I and VT-II. None produced the adhesins K99, Att25 (FY or F17) and Att111. The great majority (93.4%) of the CNF1+E coli possessed both properties. These properties allow CNF1+ to be distinguished from CNF-E coli. [less ▲]

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