References of "Ogura, Y"
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See detailIdentification of Shiga toxin-producing (STEC) and enteropathogenic (EPEC) Escherichia coli in diarrhoeic calves and comparative genomics of O5 bovine and human STEC
Fakih, Ibrahim; Thiry, Damien ULg; Duprez, Jean-Noël ULg et al

in Veterinary Microbiology (2016)

Escherichia coli producing Shiga toxins (Stx) and the attaching-effacing (AE) lesion (AE-STEC) are responsible for (bloody) diarrhoea in humans and calves while the enteropathogenic E. coli (EPEC ... [more ▼]

Escherichia coli producing Shiga toxins (Stx) and the attaching-effacing (AE) lesion (AE-STEC) are responsible for (bloody) diarrhoea in humans and calves while the enteropathogenic E. coli (EPEC) producing the AE lesion only cause non-bloody diarrhoea in all mammals. The purpose of this study was (i) to identify the pathotypes of enterohaemolysin-producing E. coli isolated between 2009 and 2013 on EHLY agar from less than 2 month-old diarrhoeic calves with a triplex PCR targeting the stx1, stx2, eae virulence genes; (ii) to serotype the positive isolates with PCR targeting the genes coding for ten most frequent and pathogenic human and calf STEC O serogroups; and (iii) to compare the MLSTypes and virulotypes of calf and human O5 AE-STEC after Whole Genome Sequencing using two server databases (www.genomicepidemiology.org). Of 233 isolates, 206 were triplex PCR-positive: 119 AE-STEC (58%), 78 EPEC (38%) and 9 STEC (4%); and the stx1+eae+ AE-STEC (49.5%) were the most frequent. Of them, 120 isolates (84% of AE-STEC, 23% of EPEC, 22% of STEC) tested positive with one O serogroup PCR: 57 for O26 (47.5%), 36 for O111 (30%), 10 for O103 (8%) and 8 for O5 (7%) serogroups. The analysis of the draft sequences of 15 O5 AE-STEC could not identify any difference correlated to the host. As a conclusion, (i) the AE-STEC associated with diarrhoea in young calves still belong to the same serogroups as previously (O5, O26, O111) but the O103 serogroup may be emerging, (ii) the O5 AE-STEC from calves and humans are genetically similar [less ▲]

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See detailComparison of bovine and human O26 EHEC strains by the Whole Genome PCR Scanning
Bardiau, Marjorie ULg; Ogura, Y.; Mainil, Jacques ULg et al

Conference (2011, December)

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See detailComparison of bovine and human O26 EHEC strains by the Whole Genome PCR Scanning
Bardiau, Marjorie ULg; Ogura, Y.; Mainil, Jacques et al

Conference (2011, December)

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See detailTyping of O26 enterohaemorrhagic and enteropathogenic Escherichia coli isolated from humans and cattle with IS621 multiplex PCR-based fingerprinting.
Mainil, Jacques ULg; Bardiau, Marjorie ULg; Ooka, T. et al

in Journal of Applied Microbiology (2011), 111(3), 773-86

AIMS: This study evaluated a typing method of O26:H11 enterohaemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC) based on the variation in genomic location and copy numbers of IS621. METHODS ... [more ▼]

AIMS: This study evaluated a typing method of O26:H11 enterohaemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC) based on the variation in genomic location and copy numbers of IS621. METHODS AND RESULTS: Two multiplex PCRs, targeting either the left (5') or right (3') IS/chromosome junction of 12 IS621 insertion sites and one PCR specific of another truncated copy, were developed. Thirty-eight amplification profiles were observed amongst a collection of 69 human and bovine O26:H11 EHEC and EPEC. Seventy-one per cent of the 45 EHEC and EPEC with identical IS621 fingerprints within groups of two, three or four isolates had >85% pulsed field gel electrophoresis (PFGE) profile similarity, including four groups of epidemiologically related EHEC or EPEC, while most of the groups had <85% similarity between each others. Epidemiologically related EHEC from each of three independent outbreaks in Japan and Belgium also exhibited identical IS621 fingerprints and PFGE profiles. CONCLUSIONS: The IS621 fingerprinting and the PFGE are complementary typing assays of EHEC and EPEC; though, the former is less discriminatory. SIGNIFICANCE AND IMPACT OF THE STUDY: The IS621 printing method represents a rapid (24 h) first-line surveillance and typing assay, to compare and trace back O26:H11 EHEC and EPEC during surveys in farms, multiple human cases and outbreaks. [less ▲]

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See detailComparison of bovine and human O26 EHEC strains by the Whole Genome PCR Scanning
Bardiau, Marjorie ULg; Ogura, Y.; Mainil, Jacques et al

Poster (2010, April)

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See detailComparison of bovine and human O26 EHEC strains by the Whole Genome PCR Scanning
Bardiau, Marjorie ULg; Ogura, Y.; Mainil, Jacques et al

Poster (2009, March)

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See detailComparison of bovine and human O26 EHEC strains by the Whole Genome PCR Scanning
Bardiau, Marjorie ULg; Ogura, Y.; Hayashi, T. et al

Conference (2008, June)

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See detailComparison of bovine and human O26 EHEC strains by the Whole Genome PCR Scanning
Bardiau, Marjorie ULg; Ogura, Y.; Hayashi, Tetsuya et al

Conference (2008, June)

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See detailComparison of bovine and human O26 EHEC strains by the Whole Genome PCR Scanning
Bardiau, Marjorie ULg; Ogura, Y.; Hayashi, T. et al

Conference (2008, March)

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See detailComparison of bovine and human O26 EHEC strains by the Whole Genome PCR Scanning
Bardiau, Marjorie ULg; Ogura, Y.; Hayashi, T. et al

Conference (2008, March)

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