References of "Nylander, Tommy"
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See detailInteraction of fengycin with stratum corneum mimicking model membranes: a calorimetry study
Eeman, Marc; Oloffson, Gerd; Sparr, Emma et al

in Colloids and Surfaces B : Biointerfaces (2014), In Press

Based on its outstanding antifungal properties, it is reasonable to believe that fengycin might be efficient to topically treat localized dermatomycoses. Since most of the fungi species involved in the ... [more ▼]

Based on its outstanding antifungal properties, it is reasonable to believe that fengycin might be efficient to topically treat localized dermatomycoses. Since most of the fungi species involved in the formation of those mycotic skin diseases colonize primarily the stratum corneum (SC), studying the interaction between fengycin and SC-mimicking lipid membranes is a primary step to determine the potential of fengycin to overcome the physical barrier of the skin. In this respect, multilamellar lipid vesicles (MLVs), with a lipid composition mimicking that of the SC, were prepared and characterized by differential scanning calorimetry (DSC). The critical micelle concentration (CMC) of fengycin was also assessed under skin conditions and found to be 1.2 ± 0.1 μM. The molecular interactions of fengycin with SC-mimicking MLVs were investigated by both DSC and isothermal titration calorimetry (ITC). Results showed that the interactions were considerably affected by changes in lipid phase behaviour. At 40 °C and below, fengycin induced exothermic changes in the lipid structures suggesting that less-ordered lipid domains became more-ordered in presence of fengycin. At 60 °C, clearly endothermic interaction enthalpies were observed, which could arise from the “melting” of remaining solid domains enriched in high melting lipids that without fengycin melt at higher temperatures. [less ▲]

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See detailInteraction of fengycin with stratum corneum mimicking model membranes: a calorimetry study
Nasir, Mehmet Nail ULg; Eeman, Marc; Olofsson, Gerd et al

in European Biophysics Journal [=EBJ] (2013), 42(S1), 168

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See detailEffects of surfactin on membrane models displaying lipid phase separation.
Deleu, Magali ULg; Lorent, Joseph; Lins, Laurence ULg et al

in Biochimica et Biophysica Acta (2013), 1828(2), 801-815

Surfactin, a bacterial amphiphilic lipopeptide is attracting more and more attention in view of its bioactive properties which are in relation with its ability to interact with lipids of biological ... [more ▼]

Surfactin, a bacterial amphiphilic lipopeptide is attracting more and more attention in view of its bioactive properties which are in relation with its ability to interact with lipids of biological membranes. In this work, we investigated the effect of surfactin on membrane structure using model of membranes, vesicles as well as supported bilayers, presenting coexistence of fluid-disordered (DOPC) and gel (DPPC) phases. A range of complementary methods was used including AFM, ellipsometry, dynamic light scattering, fluorescence measurements of Laurdan, DPH, calcein release, and octadecylrhodamine B dequenching. Our findings demonstrated that surfactin concentration is critical for its effect on the membrane. The results suggest that the presence of rigid domains can play an essential role in the first step of surfactin insertion and that surfactin interacts both with the membrane polar heads and the acyl chain region. A mechanism for the surfactin lipid membrane interaction, consisting of three sequential structural and morphological changes, is proposed. At concentrations below the CMC, surfactin inserted at the boundary between gel and fluid lipid domains, inhibited phase separation and stiffened the bilayer without global morphological change of liposomes. At concentrations close to CMC, surfactin solubilized the fluid phospholipid phase and increased order in the remainder of the lipid bilayer. At higher surfactin concentrations, both the fluid and the rigid bilayer structures were dissolved into mixed micelles and other structures presenting a wide size distribution. [less ▲]

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See detailInteraction between the antifungal lipopeptide fengycin and lipid membrane
Deleu, Magali ULg; Nylander, Tommy

Conference (2012, August 28)

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See detailInteraction between the bioactive lipopeptide fengycin and lipid membrane
Deleu, Magali ULg; Campbell, Richard; Hellsing, Maya et al

Poster (2009)

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See detailInvestigation of interactions between phospholipids, cholesterol and surfactin by atomic force microscopy and differential scanning calorimetry
Eeman, Marc; Nylander, Tommy; Dufrêne, Yves et al

Poster (2006)

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See detailInteraction of antimicrobial lipopeptides from Bacillus subtilis with lipid membranes
Deleu, Magali ULg; Paquot, Michel ULg; Olofsson, Gerd et al

Poster (2004)

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See detailInteraction of lipopeptides from Bacillus subtilis with lipid vesicles
Deleu, Magali ULg; Paquot, Michel ULg; Olofsson, Gerd et al

Poster (2004)

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