References of "Msika, Philippe"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailBone sialoprotein as a potential key factor implicated in the pathophysiology of osteoarthritis
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Walsh, David et al

in Osteoarthritis and Cartilage (2014), 22(4), 547-56

Detailed reference viewed: 10 (3 ULg)
See detailASSOCIATION BETWEEN CHONDROCYTE HYPERTROPHY AND ANGIOGENESIS OF CARTILAGE IN OSTEOARTHRITIS
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Delcour, Jean-Pierre et al

Conference (2013, November)

Detailed reference viewed: 18 (5 ULg)
See detailBONE SIALOPROTEIN AS A KEY FACTOR IN THE PATHOPHYSIOLOGY OF OSTEOARTHRITIS
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Walsh, David et al

Conference (2013, November)

Detailed reference viewed: 9 (1 ULg)
Full Text
Peer Reviewed
See detailLa bone sialoproteine: un facteur clé dans la pathogénie de l'arthrose
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Delcour, Jean-Pierre et al

Conference (2012, December)

Detailed reference viewed: 16 (4 ULg)
Full Text
Peer Reviewed
See detailAssociation entre l'hypertrophie du chondrocyte et l'angiogenèse du cartilage dans l'arthrose
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Delcour, Jean-Pierre et al

Conference (2012, December)

Detailed reference viewed: 30 (2 ULg)
Full Text
Peer Reviewed
See detailAssociation between chondrocytes hypertrophy and angiogenesis of cartilage in osteoarthritis
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Delcour, Jean-Pierre et al

Poster (2012, November 13)

Detailed reference viewed: 22 (2 ULg)
Full Text
Peer Reviewed
See detailAssociation between chondrocytes hypertrophy and angiogenesis of cartilage in osteoarthritis
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Delcour, Jean-Pierre et al

in Arthritis and Rheumatism (2012, October), 64(10 (Suppl)), 760

Detailed reference viewed: 26 (3 ULg)
Full Text
Peer Reviewed
See detailBone sialoprotein: a key mediator of the angiogenic activity of hypertrophic osteoarthritic chondrocytes
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Baudouin, Caroline et al

in Osteoarthritis and Cartilage (2012), 20(S), 122-123

Detailed reference viewed: 7 (1 ULg)
Full Text
Peer Reviewed
See detailAssociation entre l'hypertrophie du chondrocyte et l'angiogenèse du cartilage dans l'arthrose
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Delcour, Jean-Pierre et al

in Revue du Rhumatisme (2012), 79(S), 105-106

Detailed reference viewed: 6 (0 ULg)
Full Text
Peer Reviewed
See detailLa bone sialoproteine: un facteur clé dans la pathogénie de l'arthrose
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Delcour, Jean-Pierre et al

in Revue du Rhumatisme (2012), 79(S), 106

Detailed reference viewed: 1 (0 ULg)
Full Text
Peer Reviewed
See detailLa bone sialoproteine: un facteur clé dans la pathogénie de l'arthrose
Pesesse, Laurence ULg; Sanchez, Christelle ULg; Delcour, Jean-Pierre et al

in Revue du Rhumatisme (2012), 79(S), 106

Detailed reference viewed: 4 (0 ULg)
Full Text
Peer Reviewed
See detailUsefulness of specific OA biomarkers, Coll2-1 and Coll2-1NO(2), in the anterior cruciate ligament OA canine model.
Henrotin, Yves ULg; Martel-Pelletier, Johanne; Msika, Philippe et al

in Osteoarthritis and cartilage / OARS, Osteoarthritis Research Society (2012), 20(7), 787-90

Detailed reference viewed: 14 (0 ULg)
Full Text
Peer Reviewed
See detailRegulation of subchondral bone osteoblast metabolism by cyclic compression.
Sanchez, Christelle ULg; Pesesse, Laurence ULg; Gabay, Odile et al

in Arthritis and Rheumatism (2012), 64(4), 1193-203

OBJECTIVE: Recent data have shown that abnormal subchondral bone remodeling plays an important role in osteoarthritis (OA) onset and progression, and it was suggested that abnormal mechanical pressure ... [more ▼]

OBJECTIVE: Recent data have shown that abnormal subchondral bone remodeling plays an important role in osteoarthritis (OA) onset and progression, and it was suggested that abnormal mechanical pressure applied to the articulation was responsible for these metabolic changes. This study was undertaken to evaluate the effects of cyclic compression on osteoblasts from OA subchondral bone. METHODS: Osteoblasts were isolated from sclerotic and nonsclerotic areas of human OA subchondral bone. After 28 days, the osteoblasts were surrounded by an abundant extracellular matrix and formed a resistant membrane, which was submitted to cyclic compression (1 MPa at 1 Hz) for 4 hours. Gene expression was evaluated by reverse transcription-polymerase chain reaction. Protein production in culture supernatants was quantified by enzyme-linked immunosorbent assay or visualized by immunohistochemistry. RESULTS: Compression increased the expression of genes coding for interleukin-6 (IL-6), cyclooxygenase 2, RANKL, fibroblast growth factor 2, IL-8, matrix metalloproteinase 3 (MMP-3), MMP-9, and MMP-13 but reduced the expression of osteoprotegerin in osteoblasts in both sclerotic and nonsclerotic areas. Colalpha1(I) and MMP-2 were not significantly affected by mechanical stimuli. Nonsclerotic osteoblasts were significantly more sensitive to compression than sclerotic ones, but after compression, differences in messenger RNA levels between nonsclerotic and sclerotic osteoblasts were largely reduced or even abolished. Under basal conditions, sclerotic osteoblasts expressed similar levels of alpha5, alphav, beta1, and beta3 integrins and CD44 as nonsclerotic osteoblasts but 30% less connexin 43, an important mechanoreceptor. CONCLUSION: Genes involved in subchondral bone sclerosis are mechanosensitive. After compression, nonsclerotic and sclerotic osteoblasts expressed a similar phenotype, suggesting that compression could be responsible for the phenotype changes in OA subchondral osteoblasts. [less ▲]

Detailed reference viewed: 37 (1 ULg)
Full Text
Peer Reviewed
See detailMatrix metalloproteinase-3 is a highly mechanosensitive gene in human subchondral osteoblasts
Sanchez, Christelle ULg; Pesesse, Laurence ULg; Delcour, Jean-Pierre et al

in Osteoarthritis and Cartilage (2010), 17(suppl 2), 217

Detailed reference viewed: 31 (2 ULg)
Peer Reviewed
See detailEffects of compression on human subchondral osteoblast metabolism
Kesteloot, Frédéric ULg; Gabay, Odile; Msika, Philippe et al

Poster (2009, May 24)

Introduction. Recent data showed that subchondral bone plays an important role in osteoarthritis (OA). Metabolic and morphologic modifications in this tissue contribute to the degradation of the ... [more ▼]

Introduction. Recent data showed that subchondral bone plays an important role in osteoarthritis (OA). Metabolic and morphologic modifications in this tissue contribute to the degradation of the overlaying cartilage. It was suggested that abnormal mechanical pressure exerted onto the articulation was responsible to these changes. Here, we evaluated the effects of compression on osteoblasts from subchondral bone. Method. Osteoblasts were isolated from sclerotic (SC) or non-sclerotic (NSC) areas of human OA subchondral bone. After 28 days, osteoblasts were surrounded by their matrix. This osteoblasts-containing membrane was then placed onto a Biopress Flexercell plate and submitted to a 4h 1.67 MPa compression (1 Hz). Expression of IL-6, IL-8, COX-2, VEGF, IGF-1, OPG and RANKL was evaluated by RT-PCR. IL-6, IL-8 and PGE2 were quantified by ELISA. Results. Basal IL-6, VEGF, COX-2, IGF-1 and RANKL mRNA levels were significantly increased in SC osteoblasts as compared to NSC. By contrast, SC osteoblasts expressed less OPG than those from NSC areas. Compressions induced the expression of genes coding for IL-6, IL-8, COX-2, IGF-1, VEGF and RANKL but decreased the expression of OPG in NSC osteoblasts (p<0.01). Interestingly, compressed NSC osteoblasts expressed similar levels of these genes than SC osteoblasts. Conclusions. We show that our model of compression can induce in NSC osteoblasts a phenotype similar to this observed in sclerotic areas. Moreover, SC osteoblasts are less sensitive to mechanical stimuli than NSC osteoblasts. These results clarify the role of compression in the pathogenesis of subchondral bone sclerosis and allow new perspectives of research in this field. [less ▲]

Detailed reference viewed: 63 (1 ULg)
Peer Reviewed
See detailFollow-up of COLL2-1, COLL2-1NO2 and myeloperoxydase in dogs after transection of the cruciate ligament of the knee
Kesteloot, Frédéric ULg; Pelletier, Jean-Pierre; Martel-Pelletier, Johanne et al

Poster (2009, May 23)

Purpose: To determine the profile of Coll2-1, Coll2-1NO2 and myeloperoxydase (MPO) serum concentrations in experimental knee OA induced in the dog by transection of the anterior cruciate ligament. Methods ... [more ▼]

Purpose: To determine the profile of Coll2-1, Coll2-1NO2 and myeloperoxydase (MPO) serum concentrations in experimental knee OA induced in the dog by transection of the anterior cruciate ligament. Methods: Surgical transection of the ACL of the right knee was performed on 16 adult crossbred dogs. The dogs were sacrificed 8 weeks after the surgical procedure. Coll2-1, Coll2-1NO2 and MPO were measured by specific immunoassays in 16 dogs at baseline and every 2 weeks during the 8 weeks. The results were expressed as median (range). Results: Immunostainings with D3 and D37, the antiserum recognizing Coll2-1 and Coll2-1NO2, respectively, labelled extracellular matrix in the superficial layer of fibrillated cartilage. After the transection of the ACL, the concentration of 3 biomarkers increased significantly (Friedman test: p<0.001). The concentrations of Coll2-1 and MPO were significantly increased at week 2 compared to baseline [Coll2-1 baseline: 281.57 (131.02-384.67) nM vs Coll2-1 week 2: 345.52 (181.15-589.25) nM (p<0.01) and MPO baseline: 5.16 (<0.4-14.7) ng/ml vs MPO week 2: 14.54 (3.28-31.50) ng/ml (p<0.001)] and remained stable until week 8 [Coll2-1 week 8:318.89 (117.95-492.28) nM and MPO week 8: 11.55 (2.87-42.94) ng/ml]. The Coll2-1NO2 concentration increased significantly at weeks 6 and 8 compared to baseline [Coll2-1NO2 baseline: 0.54 (0.29-1.48) nM vs Coll2-1NO2 week 6: 0.64 (0.40-1.9) nM (p<0.001) vs week 8: 0.61 (0.37-1.79) nM]. Conclusions: These findings suggest that Coll2-1 is a relevant marker for the detection of early structural changes in OA dogs. Interestingly, MPO and Coll2-1NO2 are increased in OA dogs indicating that an oxidative stress occurs in this OA model. [less ▲]

Detailed reference viewed: 53 (7 ULg)