  Organometallic-mediated radical polymerization of vinyl amides: Effect of metal coordinationKermagoret, Anthony  ; ; Hurtgen, Marie et alPoster (2012, September 04) Detailed reference viewed: 12 (0 ULg)Organometallic-mediated radical polymerization of vinyl amides: Effect of metal coordinationKermagoret, Anthony ; ; Hurtgen, Marie et alConference (2012, September 04) Detailed reference viewed: 35 (2 ULg) Effects of consolidation of procedural motor memory traces on slow and fast spindles; ; et al Poster (2009, April) Detailed reference viewed: 1 (0 ULg)Triiodothyronine inhibits transcription from the human growth hormone promoter; ; Voz, Marianne et alin Molecular & Cellular Endocrinology (1990), 71(3), 261-7 Three DNA constructs, the natural human growth hormone gene (hGH-hGH) its 500 bp promoter linked to the chloramphenicol acetyl transferase reporter gene (hGH-CAT), and its structural part linked to the ... [more ▼] Three DNA constructs, the natural human growth hormone gene (hGH-hGH) its 500 bp promoter linked to the chloramphenicol acetyl transferase reporter gene (hGH-CAT), and its structural part linked to the herpes virus thymidine kinase promoter (TK-hGH) were introduced into rat pituitary GC cells by DEAE-dextran transfection. Transient expression was followed as a function of triiodothyronine (T3) concentration. The hGH-CAT expression was specifically inhibited by T3 following a typical dose-response curve while hGH-GH gene expression was not significantly modified. The transient expression of TK-hGH increased as a function of T3 concentration. These results indicate that T3 exerts two opposite effects on hGH gene expression. First, it down-regulates expression by acting on the promoter; second, it up-regulates expression by acting on the structural part of the gene. These action could be due to regulation of transcription and mRNA stabilization, respectively. [less ▲] Detailed reference viewed: 28 (0 ULg)Location of transcriptional regulatory sequences within the prolactin family genes.; ; et al in Neuroendocrine Perspectives. (1986) Localisation des séquences régulatrices de la transcription. Application aux gènes de la famille prolactine.; ; et al in Annales d'Endocrinologie (1986), 47 We are studying nucleotide sequences responsible for the regulation of eukaryotic gene expression. Our test system comprises the human genes coding for prolactin (hPRL), growth hormone (hGH-N) and ... [more ▼] We are studying nucleotide sequences responsible for the regulation of eukaryotic gene expression. Our test system comprises the human genes coding for prolactin (hPRL), growth hormone (hGH-N) and placental lactogen (hCS-B). We have cloned these genes and are searching within their sequences for in vitro binding sites of the human glucocorticoid receptor on the hGH-N and hCS-B genes; the in vivo activity of such DNA sequences by assaying hybrid gene expression in transfected cells; in vivo "enhancer" activity of different hPRL gene fragments linked to a marker gene and transfected in cultured cells. [less ▲] Detailed reference viewed: 11 (0 ULg)Localisation des séquences régulatrices de la transcription. Application aux gènes de la famille de la prolactine.; ; et al in Annales d'Endocrinologie (1986), 47 We are studying nucleotide sequences responsible for the regulation of eukaryotic gene expression. Our test system comprises the human genes coding for prolactin (hPRL), growth hormone (hGH-N) and ... [more ▼] We are studying nucleotide sequences responsible for the regulation of eukaryotic gene expression. Our test system comprises the human genes coding for prolactin (hPRL), growth hormone (hGH-N) and placental lactogen (hCS-B). We have cloned these genes and are searching within their sequences for in vitro binding sites of the human glucocorticoid receptor on the hGH-N and hCS-B genes; the in vivo activity of such DNA sequences by assaying hybrid gene expression in transfected cells; in vivo "enhancer" activity of different hPRL gene fragments linked to a marker gene and transfected in cultured cells. [less ▲] Detailed reference viewed: 24 (6 ULg)Location of transcriptional and regulatory sequences within the prolactin family genes; ; et al in Müller, E. E.; McLeod, R. M. (Eds.) Neuroendocrine Perspectives (1986) Detailed reference viewed: 5 (2 ULg)Inverse control of prolactin and growth hormone gene expression: effect of thyroliberin on transcription and RNA stabilization; ; et al in EMBO Journal (1983), 2(9), 1493-9 The hypothalamic tripeptide thyroliberin (TRH) regulates prolactin (PRL) and growth hormone (GH) synthesis inversely by modulating the levels of their specific mRNA. Changes in mRNA levels could involve ... [more ▼] The hypothalamic tripeptide thyroliberin (TRH) regulates prolactin (PRL) and growth hormone (GH) synthesis inversely by modulating the levels of their specific mRNA. Changes in mRNA levels could involve both transcriptional and posttranscriptional events. To examine further these possibilities, we have investigated the effect of TRH on the biosynthesis and degradation of PRL and GH RNA in a rat pituitary tumor cell line. Newly synthesized PRL and GH RNA sequences were quantified in nuclear and cytoplasmic fractions by hybridization of 3H-labelled RNA to immobilized plasmid DNA containing either PRL or GH cDNA sequences. Steady-state levels of specific RNA were estimated by RNA blot hybridization. The results indicate that TRH increases in a rapid but transient manner the transcription of the PRL gene, and suggest that it does not alter the processing and the transport to the cytoplasm. In contrast, after a lag-time, TRH seems to induce a long-lasting inhibition on GH, as well as on overall gene transcription. Furthermore, we observed an effect of TRH on mRNA stability. TRH significantly increases the half-life of PRL mRNA. Our results also support the hypothesis that TRH decreases the half-life of GH mRNA. Such post-transcriptional action of TRH amplifies and prolongs the regulations exerted at the transcriptional level. [less ▲] Detailed reference viewed: 5 (0 ULg)Structure et régulation du gène de la prolactineMartial, Joseph ; ; in Gautray, J. P.; Guillon, G. (Eds.) Prolactine neurotransmission et fertilité (1982) Detailed reference viewed: 29 (1 ULg)Structure et régulation du gène de la prolactineMartial, Joseph ; ; (1982) Detailed reference viewed: 2 (0 ULg) |
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