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See detailUne famille de minisatellites hypervariables detectee au moyen d'une sequence derivee du phage M 13.
Brocas, H.; Georges, Michel ULg; Christophe, D. et al

in Comptes Rendus de l'Académie des Sciences. Série III, Sciences de la Vie (1987), 304(3), 67-9

A new family of "hypervariable minisatellites" has been identified in the genome of man and of a variety of animal species using as a probe a DNA segment isolated from the M 13 bacteriophage. This finding ... [more ▼]

A new family of "hypervariable minisatellites" has been identified in the genome of man and of a variety of animal species using as a probe a DNA segment isolated from the M 13 bacteriophage. This finding provides a new series of hyperpolymorphic genetic markers and renders the "DNA-fingerprinting" methodology available to every molecular biology laboratory. [less ▲]

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See detailA sequence in M13 phage detects hypervariable minisatellites in human and animal DNA.
Vassart, G.; Georges, Michel ULg; Monsieur, R. et al

in Science (1987), 235(4789), 683-4

The term "DNA fingerprint" has been used to describe the extensive restriction fragment length polymorphism associated with hypervariable minisatellites present in the human genome. Until now, it was ... [more ▼]

The term "DNA fingerprint" has been used to describe the extensive restriction fragment length polymorphism associated with hypervariable minisatellites present in the human genome. Until now, it was necessary to hybridize Southern blots to specific probes cloned from human genomic DNA in order to obtain individual-specific restriction patterns. The present study describes the surprising finding that the insert-free, wild-type M13 bacteriophage detects hypervariable minisatellites in human and in animal DNA, provided no competitor DNA is used during hybridization. The effective sequence in M13 was traced to two clusters of 15-base pair repeats within the protein III gene of the bacteriophage. This unexpected use of M13 renders the DNA fingerprinting technology more readily available to molecular biology laboratories. [less ▲]

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