   The dermatophyte species Arthroderma benhamiae: intraspecies variability and mating behaviour; ; et al in Journal of Medical Microbiology (2013), 62 Detailed reference viewed: 15 (4 ULg) Mechanisms of skin adherence and invasion by dermatophytesBaldo, Aline  ; ; Mathy, Anne et alin Mycoses (2012), 55(3), 218-223 Detailed reference viewed: 27 (8 ULg)Zoonotic infections with dermatophyte fungiMignon, Bernard ; in Palmer, S.; Soulsby, L.; Torgerson, P. (Eds.) et al Oxford Textbook of Zoonoses - Biology, Clinical Practice, and Public Health Control (2011) Detailed reference viewed: 58 (32 ULg) Characterization of a new potential virulence factor of Microsporum canis, the secreted subtilisin Sub6Mathy, Anne ; Baldo, Aline ; et alin Mycoses (2011), 54(suppl 2), 112-113 Detailed reference viewed: 94 (29 ULg)Identification of novel secreted proteases during extracellular proteolysis by dermatophytes at acidic pH; ; et al in Proteomics (2011) Detailed reference viewed: 18 (5 ULg)Targeted gene deletion and in vivo analysis of putative virulence gene function in the pathogenic dermatophyte Arthroderma benhamiae; Defaweux, Valérie ; Mignon, Bernard et alin Eukaryotic Cell (2011), 10(6), 842-853 Detailed reference viewed: 20 (13 ULg)Molecular analysis and mating behaviour of the Trichophyton mentagrophytes species complex; ; et al in International Journal of Medical Microbiology (2011), 301(3), 260-266 Detailed reference viewed: 25 (10 ULg)Differential gene expression in the pathogenic dermatophyte Arthroderma benhamiae in vitro versus during infection; ; Mignon, Bernard et alin Microbiology (2010), 156(3), 884-895 Detailed reference viewed: 12 (6 ULg)Pets as the main source of two zoonotic species of the Trichophyton mentagrophytes complex in Switzerland, Arthroderma vanbreuseghemii and Arthroderma benhamiae; Mignon, Bernard ; et alin Veterinary Dermatology (2009), 20(1), 13-18 Detailed reference viewed: 16 (4 ULg)RNA silencing in the dermatophyte Microsporum canis; ; Baldo, Aline et alin FEMS Microbiology Letters (2007), 275(1), 38-45 Dermatomycoses caused by Microsporum canis are frequent in domestic animals and easily transmissible to humans. Several proteases secreted by this fungus were identified as potential virulence factors ... [more ▼] Dermatomycoses caused by Microsporum canis are frequent in domestic animals and easily transmissible to humans. Several proteases secreted by this fungus were identified as potential virulence factors, but the construction of deficient strains is required to investigate their role in the pathogenesis of the disease. Using target genes encoding two of these proteases, a first evaluation of the utility of RNA-mediated silencing as a reverse genetic tool in dermatophytes was carried out. SUB3 and DPPIV, respectively coding for a subtilisin and a dipeptidyl peptidase, were both down-regulated, by means of two plasmid constructs designed to express an RNA hairpin that corresponds to part of their respective sequence. The degree of attenuation was evaluated by enzymatic assay of the transformants culture supernatants, and by real-time reverse transcriptase-polymerase chain reaction. Enzymatic activities and expression levels varied from less than 5% to 100% of that of control transformants obtained with plasmid without hairpin inserts. Inhibition was globally more efficient for SUB3 than for DPPIV. These results show that RNA silencing can be used for functional genomics in M. canis, and particularly to circumvent the limits and technical difficulties of conventional disruption methods. [less ▲] Detailed reference viewed: 61 (5 ULg)Secreted subtilisin gene family in Trichophyton rubrum; ; et al in Gene (2004), 339 Secreted proteases constitute potential virulence factors of dermatophytes. A total of seven genes encoding putative serine proteases of the subtilisin family (SUB) were isolated in Trichophyton rubrum ... [more ▼] Secreted proteases constitute potential virulence factors of dermatophytes. A total of seven genes encoding putative serine proteases of the subtilisin family (SUB) were isolated in Trichophyton rubrum. Based on sequence data and intron-exon structure, a phylogenetic analysis of subtilisins from T rubrum and other fungi revealed a presumed ancestral lineage comprising T rubrum SUB2 and Aspergillus SUBs. All other SUBs (SUB1, SUB3-7) are dermatophyte-specific and have apparently emerged more recently, through successive gene duplication events. We showed that two subtilisins, Sub3 and Sub4, were detected in culture supernatants of T rubrum grown in a medium containing soy protein as a sole nitrogen source. Both recombinant enzymes produced in Pichia pastoris are highly active on keratin azure suggesting that these proteases play an important role in invasion of keratinised tissues by the fungus. The set of deduced amino acid sequences of T rubrum SUB ORFs allowed the identification of orthologous Subs secreted by other dermatophyte species using proteolysis and mass spectrometry. (C) 2004 Elsevier B.V. All rights reserved. [less ▲] Detailed reference viewed: 47 (1 ULg)Multiplication of an ancestral gene encoding secreted fungalysin preceded species differentiation in the dermatophytes Trichophyton and Microsporum; ; et al in Microbiology-Sgm (2004), 150(Part 2), 301-310 Dermatophytes are human and animal pathogenic fungi which cause cutaneous infections and grow exclusively in the stratum corneum, nails and hair. In a culture medium containing soy proteins as sole ... [more ▼] Dermatophytes are human and animal pathogenic fungi which cause cutaneous infections and grow exclusively in the stratum corneum, nails and hair. In a culture medium containing soy proteins as sole nitrogen source a substantial proteolytic activity was secreted by Trichophyton rubrum, Trichophyton mentagrophytes and Microsporum canis. This proteolytic activity was 55-75% inhibited by o-phenanthroline, attesting that metalloproteases were secreted by all three species. Using a consensus probe constructed on previously characterized genes encoding metalloproteases (MEP) of the M36 fungalysin family in Aspergillus fumigatus, Aspergillus oryzae and M. canis, a five-member MEP family was isolated from genomic libraries of T rubrum, T mentagrophytes and M. canis. A phylogenetic analysis of genomic and protein sequences revealed a robust tree consisting of five main clades, each of them including a MEP sequence type from each dermatophyte species. Each MEP type was remarkably conserved across species (72-97% amino acid sequence identity). The tree topology clearly indicated that the multiplication of MEP genes in dermatophytes occurred prior to species divergence. In culture medium containing soy proteins as a sole nitrogen source secreted Meps accounted for 19-36% of total secreted protein extracts; characterization of protein bands by proteolysis and mass spectrometry revealed that the three dermatophyte species secreted two Meps (Mep3 and Mep4) encoded by orthologous genes. [less ▲] Detailed reference viewed: 22 (3 ULg)In vivo detection of three Microsporum canis subtilisin-like serine protease mRNAs in infected guinea-pigs; ; et al Conference (2003) Detailed reference viewed: 7 (2 ULg)Evaluation of the specific immune response against a recombinant keratinase of Microsporum canis; ; et al Conference (2003) Detailed reference viewed: 4 (1 ULg)In vivo expression of a Microsporum canis 43.5 kDa metalloprotease in infected guinea pigs; ; et al Poster (2002) Detailed reference viewed: 13 (3 ULg)Transcription of the Microsporum canis 31.5 kDa keratinase gene in infected guinea-pigs; ; et al Poster (2002) Detailed reference viewed: 10 (1 ULg) |
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