 Immunohistochemical demonstration of laminin, the major glycoprotein of basement membranes, as an aid in the diagnosis of soft tissue tumors.; Foidart, Jean-Michel  ; in American Journal of Clinical Pathology (1983), 79(3), 306-11 Forty-eight benign and malignant soft tissue tumors were investigated immunohistologically for the presence of laminin, a glycoprotein of basement membranes. The results showed intense laminin positivity ... [more ▼] Forty-eight benign and malignant soft tissue tumors were investigated immunohistologically for the presence of laminin, a glycoprotein of basement membranes. The results showed intense laminin positivity in schwannomas and neurofibromas and less intense positivity in leiomyomas and leiomyosarcomas, whereas fibrous histiocytomas and fibrosarcomas generally were negative. It is suggested that immunohistochemical demonstration of laminin is a valuable aid in the differential diagnosis between tumors derived from fibroblasts and Schwann cells, and a potential aid in the differential diagnosis between tumors originating from fibroblasts and those from smooth muscle cells. In addition to the expression seen in the tumors, all vascular walls were positive for laminin. Therefore, demonstration of laminin also can be used to examine the vascular pattern of tumors. [less ▲] Demonstration of laminin, a basement membrane glycoprotein, in routinely processed formalin-fixed human tissues.; ; et al in Histochemistry (1982), 75(3), 301-7 Laminin was demonstrated by immunoperoxidase and immunofluorescence staining in sections of normal human tissues fixed in formalin and routinely processed in paraffin. Exposure of the sections to a ... [more ▼] Laminin was demonstrated by immunoperoxidase and immunofluorescence staining in sections of normal human tissues fixed in formalin and routinely processed in paraffin. Exposure of the sections to a solution of pepsin (Burns et al. (1980) Histochemistry 67:73-78) revealed the antigenicity of this basement membrane glycoprotein. Sections from paraffin blocks stored for years at room temperature could be stained with this procedure. Normal human tissues, developing fetal tissues and tumors could be stained with this method. The staining patterns were similar to those seen in unfixed frozen sections. It thus appears that basement membrane components can be detected by immunohistological means from routinely processed histological samples, once the sections are pretreated with proteases. Staining for laminin could be used in embryonic studies and in histopathology to study the relation of cells to basement membranes and for the visualization of normal and abnormal vascularization. [less ▲] Detailed reference viewed: 11 (0 ULg) |
||
