References of "Michaux, Johan"
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See detailLiver microbiome of Peromyscus leucopus, a key reservoir host species for emerging infectious diseases in North America
André, Adrien ULiege; Mouton, Alice ULiege; millien, virginie et al

in Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases (2017)

Microbiome studies generally focus on the gut microbiome, which is composed of a large proportion of commensal bacteria. Here we propose a first analysis of the liver microbiomeusing next generation ... [more ▼]

Microbiome studies generally focus on the gut microbiome, which is composed of a large proportion of commensal bacteria. Here we propose a first analysis of the liver microbiomeusing next generation sequencing as a tool to detect potentially pathogenic strains. We used Peromyscus leucopus, the main reservoir host species of Lyme disease in eastern North America, as a model and sequenced V5-V6 regions of the 16S gene from 18 populations in southern Quebec (Canada). The Lactobacillus genus was found to dominate the liver microbiome.We also detected a large proportion of individuals infected by Bartonella vinsonii arupensis, a human pathogenic bacteria responsible for endocarditis, aswell as Borrelia burgdorferi, the pathogen responsible for Lyme disease in North America. We then compared the microbiomes among two P. leucopus genetic clusters occurring on either side of the St. Lawrence River, and did not detect any effect of the host genotype on their liver microbiome assemblage. Finally, we report, for the first time, the presence of B. burgdorferi in a smallmammal host fromthe northern side of the St. Lawrence River, in support of models that have predicted the northern spread of Lyme disease in Canada. [less ▲]

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See detailEffects of parasite and historic driven selection on the diversity and structure of a MHC-II gene in a small mammal species (Peromyscus leucopus) undergoing range expansion
andré, adrien; millien, Virginie; Galan, Maxime et al

in Evolutionary Ecology (2017), DOI 10.1007/s10682-017-9898-z

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See detailEvidence of a fine-scale genetic structure for the endangered Pyrenean desman (Galemys pyrenaicus) in the French Pyrenees
Gillet, F; Cabria, M.; Blanc, F. et al

in Journal of Mammalogy (2017)

The Pyrenean desman (Galemys pyrenaicus) is a small, semi-aquatic mammal endemic to the Pyrenean Mountains and the northern half of the Iberian Peninsula where it lives in cold and well-oxygenated flowing ... [more ▼]

The Pyrenean desman (Galemys pyrenaicus) is a small, semi-aquatic mammal endemic to the Pyrenean Mountains and the northern half of the Iberian Peninsula where it lives in cold and well-oxygenated flowing mountain streams. This species is currently classified as vulnerable on the IUCN Red List and has been undergoing habitat loss and fragmentation for decades, inevitably impacting its distribution. A recent genetic study, based on mitochondrial and intronic sequences, showed that the genetic variability of the Pyrenean desman is very low in the Pyrenees. In this study, we investigated the potential existence of genetic structure and gene flow at a smaller scale using 24 polymorphic microsatellite loci. As the Pyrenean desman is a very elusive species, we supplemented our tissue sample collection with samples of feces collected in the French range of this species. We successfully identified 70 individuals based on 355 fecal samples. Bayesian analyses revealed 3 genetic and geographic clusters (1 eastern, 1 central, and 1 western, including 3 genetic sub-clusters), with origins tracing back only 200 years. These clusters were characterized by low levels of genetic diversity and high inbreeding coefficients. Although gene flow among clusters appeared to be limited, populations seem to have exchanged alleles recently. Therefore, connectivity between watersheds should be enhanced to maintain genetic diversity and potentially improve the long-term survival of the Pyrenean desman in France [less ▲]

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See detailHigh gene flow between alternative morphs and the evolutionary persistence of facultative paedomorphosis
Oromi Farrús, Neus ULiege; Michaux, Johan ULiege; Denoël, Mathieu ULiege

in Scientific Reports (2016), 6

Paedomorphosis and metamorphosis are two major developmental processes that characterize the evolution of complex life cycles in many lineages. Whereas these processes were fixed in some taxa, they ... [more ▼]

Paedomorphosis and metamorphosis are two major developmental processes that characterize the evolution of complex life cycles in many lineages. Whereas these processes were fixed in some taxa, they remained facultative in others, with alternative phenotypes expressed in the same populations. From a genetic perspective, it is still unknown whether such phenotypes form a single population or whether they show some patterns of isolation in syntopy. This has deep implications for understanding the evolution of the phenotypes, i.e. towards their persistence or their fixation and speciation. Newts and salamanders are excellent models to test this hypothesis because they exhibit both developmental processes in their populations: the aquatic paedomorphs retain gills, whereas the metamorphs are able to colonize land. Using microsatellite data of coexisting paedomorphic and metamorphic palmate newts (Lissotriton helveticus), we found that they formed a panmictic population, which evidences sexual compatibility between the two phenotypes. The high gene flow could be understood as an adaptation to unstable habitats in which phenotypic plasticity is favored over the fixation of developmental alternatives. This makes then possible the persistence of a polyphenism: only metamorphosis could be maintained in case of occasional drying whereas paedomorphosis could offer specific advantages in organisms remaining in water. [less ▲]

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See detailEvidence of Predation and possible competition among three semi aquatic species, the endangered Pyrenean desman (Galemys pyrenaicus), the aquatic shrew (Neomys fodiens) and the European otter (Lutra lutra) using next-generation sequencing methods from faeces
Gillet, François; Mouton, Alice ULiege; Vanutryve, Sophie et al

Poster (2016, May)

The Pyrenean desman (Galemys pyrenaicus) is a small-endangered semi-aquatic mammal endemic to the Pyrenean Mountains and to the northern half of the Iberian Peninsula whose ecology and biology are still ... [more ▼]

The Pyrenean desman (Galemys pyrenaicus) is a small-endangered semi-aquatic mammal endemic to the Pyrenean Mountains and to the northern half of the Iberian Peninsula whose ecology and biology are still poorly known. Its ecological relationships with other semi aquatic mammals like the aquatic shrew (Neomys fodiens) or the European otter (Lutra lutra) are also unknown. The aim of this study was to analyse Pyrenean desman, aquatic shrew and European otter faeces from the same French Pyrenean regions, in order to analyze their respective diets and to detect possible predation or competition patterns among them. To study precisely their diet, we used next-generation sequencing methods (Ion Torrent or Ilumina miseq technologies). We amplified and sequenced a small DNA minibarcode (133bp) of the COI gene in 445 faeces samples from the three species. In the Pyrenean desman faeces, we were able to identify a large set of prey species with a positive match (more than 98% of identity with a reference sequence). These species belonged to four orders and eleven families among which Trichoptera and Hydropsychidae were the most frequent, respectively. The aquatic shrew diet evidenced similar aquatic preys as compared to the Pyrenean Desman. However, it also evidenced an important part of terrestrial invertebrates like woodlouse, ants, coleopters or myriapods. The otter diet was mainly constituted of fishes (eg. Cyprinidae, Salmonidae). However, several faeces samples evidenced Pyrenean Desman DNA, strongly suggesting that otter predate Pyrenean Desman in the studied areas. Future studies will be developed on a better sampling from these three species, in order to gain a better knowledge of their local diets and their ecological relationships. Such information is of great importance to propose the best management measures for the conservation of these protected species. [less ▲]

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See detailIs adult translocation a credible way to accelerate the recolonization process of Chondrostoma nasus in a rehabilitated river?
Ovidio, Michaël ULiege; Hanzen, Céline; Gennotte, Vincent ULiege et al

in Cybium (2016), 40(1), 43-49

The decline of the patrimonial rheophilic nase, Chondrostoma nasus (Linnaeus, 1758) populations was mainly caused by construction of dams and hydroelectric power-plants, together with the straightening ... [more ▼]

The decline of the patrimonial rheophilic nase, Chondrostoma nasus (Linnaeus, 1758) populations was mainly caused by construction of dams and hydroelectric power-plants, together with the straightening and artificialization of the river banks and water pollution. In this study, we tested the hypothesis whether the translocation of few adult nase individuals from a river stretch to another upstream may be a credible way to accelerate the recolonization process of the species in the Amblève River (Southern Belgium). In February and March 2011, just before their spawning period, eight adult nases (462-509 mm; 1546-2002 g; presumed males and females) were captured in the lower part of the River Amblève. Fin clip samples were stored in alcohol for further genetic analysis. They were equipped with a 14 g radio transmitter and translocated upstream in a 18 km river stretch, where the species had disappeared since decades due to river anthropization. They were manually located two to five times/week using mobile receivers until maximum June 2012 (n = 977 locations). River temperature and flow were hourly recorded during the entire tracking period. The tagged nase individuals displayed various mobility patterns, exploited different areas of the river stretch, occupied longitudinal home ranges from 3.4 to 36.1 km (one individual finally left the new river stretch) and travelled total distances from 12.2 to 186.6 km. The tagged individuals were most of the times apart from one to another, but most individuals grouped together in potential spawning areas in late March-early April 2011, suggesting an attempt to reproduce. In September 2011, electric fishing in two potential detected spawning sites allowed to capture 16 juvenile (0+) nases, demonstrating the existence of spawning activity in the newly occupied river stretch. Individual genetic characterization was performed in 2014 in order to reveal a possible direct lineage between juveniles and adults. Allelic distribution of 22 microsatellite markers unambiguously identified the 16 juveniles as full-sib progeny descending from two of the translocated adults. This demonstrated that the adult nases succeeded to find spawning areas and that progeny found raised-up from the translocated individuals. [less ▲]

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See detailEvolutionary history and species delimitations: a case study of the hazel dormouse, Muscardinus avellanarius
Mouton, Alice ULiege; Mortelliti, A.; Grill, A. et al

in Conservation Genetics (2016)

Robust identification of species and significant evolutionary units (ESUs) is essential to implement appropriate conservation strategies for endangered species. However, definitions of species or ESUs are ... [more ▼]

Robust identification of species and significant evolutionary units (ESUs) is essential to implement appropriate conservation strategies for endangered species. However, definitions of species or ESUs are numerous and sometimes controversial, which might lead to biased conclusions, with serious consequences for the management of endangered species. The hazel dormouse, an arboreal rodent of conservation concern throughout Europe is an ideal model species to investigate the relevance of species identification for conservation purposes. This species is a member of the Gliridae family, which is protected in Europe and seriously threatened in the northern part of its range. We assessed the extent of genetic subdivision in the hazel dormouse by sequencing one mitochondrial gene (cytb) and two nuclear genes (BFIBR, APOB) and genotyping 10 autosomal microsatellites. These data were analysed using a combination of phylogenetic analyses and species delimitation methods. Multilocus analyses revealed the presence of two genetically distinct lineages (approximately 11 % cytb genetic divergence, no nuclear alleles shared) for the hazel dormouse in Europe, which presumably diverged during the Late Miocene. The phylogenetic patterns suggests that Muscardinus avellanarius populations could be split into two cryptic species respectively distributed in western and central-eastern Europe and Anatolia. However, the comparison of several species definitions and methods estimated the number of species between 1 and 10. Our results revealed the difficulty in choosing and applying an appropriate criterion and markers to identify species and highlight the fact that consensus guidelines are essential for species delimitation in the future. In addition, this study contributes to a better knowledge about the evolutionary history of the species. [less ▲]

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See detailNew insight in the evolutionary history of the common dormouse, Muscardinus avellanarius: a new cryptic species?
Mouton, alice; Grill, A; Mortelliti, A et al

in Conservation Genetics (2016)

Robust identification of species entities and evolutionary units is essential to implement appropriate conservation strategies for endangered species. However, definitions of species or evolutionary units ... [more ▼]

Robust identification of species entities and evolutionary units is essential to implement appropriate conservation strategies for endangered species. However, definitions of species or evolutionary units are numerous and sometimes controversial, which might lead to biased conclusions, with serious consequences for the management of endangered species. The hazel dormouse, an arboreal rodent of conservation concern throughout Europe is an ideal model species to investigate the relevance of species identification for conservation purposes. This species is a member of the Gliridae family, which is protected in Europe and seriously threatened in the northern part of its range. We assessed the extent of genetic subdivision in the hazel dormouse by sequencing one mitochondrial gene (cytb) and two nuclear genes (BFIBR, APOB) and genotyping 10 autosomal microsatellites. These data were analysed using a combination of phylogenetic analyses and species delimitation methods. Multilocus analyses revealed the presence of two genetically distinct (approximately 11% cyt b genetic divergence, no nuclear alleles shared) lineages for the hazel dormouse in Europe, which presumably diverged during the Late Miocene. The phylogenetic patterns suggests that M. avellanarius populations could be split into two cryptic species respectively distributed in western and central-eastern Europe and Anatolia. However, the comparison of several species definitions and methods estimated the number of species between 1 and 10. Our results revealed the difficulty in choosing and applying an appropriate criterion and markers to identify species and highlight the fact that consensus guidelines are essential for species delimitation in the future. In addition, this study contributes to a better knowledge about the evolutionary history of the species. [less ▲]

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See detailUsing Next Generation Sequencing to characterize species diets: a study case with faeces of wild boar in the Spanish Pyrenees
Mouton, Alice ULiege; Espelta, Jose Maria; Baurain, Denis ULiege et al

Poster (2016)

The genomic era offer an exciting opportunity to establish biodiversity assessment for plants and animals. More specifically, DNA metabarcoding can be used as a proxy for the biodiversity existing in a ... [more ▼]

The genomic era offer an exciting opportunity to establish biodiversity assessment for plants and animals. More specifically, DNA metabarcoding can be used as a proxy for the biodiversity existing in a sample (soil, water, faeces,..) and therefore it represents a powerful and a non invasive tool to answer concerns regarding ecological questions (conservation, restoration). To illustrate such prospect, we conducted a study on the expanding wild boar populations in the Spanish Pyrenees. Over the last decades, the populations of wild boar are growing in the Iberian peninsula mainly due to their high adaptability to new environments, new climates, varied diets and high reproduction rates. Despite the economic interest of the species in the Peninsula, this expansion cause many damage on ecosystems, humans and farming practices. By moving higher in altitude, it has been suggested that the wild boar populations might be responsible for the rapid decline of the endangered capercaillie (Tetrao urogallus) populations in the Pyrenees. By searching for food, they come accross the ground nesting bird nest and gobble the eggs. The aim of this study was therefore to analyze the diet of the wild boar populations in higher altitude in the Spanish Pyrenees. 81 faecal samples collected during summer and autumn 2014 in the Aigues Tortes National Park have been extracted, amplified and sequenced using the Illumina Technology. With this method, a precise estimation of the animal prey existing in the wild boar faeces was possible and will eventually allow wild life managers to apply concrete management measures for this expanding species. [less ▲]

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See detailGenetic variations of Turkish bank vole, Myodes glareolus (Mammalia: Rodentia) inferred from mtDNA
Colak, Ercument; Karacan, GO; Kandemir, I. et al

in Mitochondrial DNA: The Journal of DNA Mapping, Sequencing, and Analysis (2016)

The bank vole, Myodes glareolus, lives in deciduous forests throughout the Palearctic region. In Turkey, this species is distributed only in northern Anatolia (the Black Sea region) where these forests ... [more ▼]

The bank vole, Myodes glareolus, lives in deciduous forests throughout the Palearctic region. In Turkey, this species is distributed only in northern Anatolia (the Black Sea region) where these forests exist. This study reveals genetic differentiation among bank vole populations based on two regions of mitochondrial DNA (cytochrome b and D-loop). Populations in northern Anatolia are divided into two genetic lineages (the ‘‘eastern’’ and ‘‘western Black Sea’’ lineages) by the Kızılırmak Valley. While the western Black Sea lineage is close to the Balkan lineage, in accordance with their geographical proximities, surprisingly, the Uludag lineage, also situated in Western Turkey appears related to the eastern Black Sea population. The divergence time analyses suggest a separation between the Balkan and Turkish groups around 0.26 Mya, whereas the split between the eastern and western Black sea lineages appeared a little bit later (0.20 Mya). Our results suggest that regional refuges existed for this species in Turkey and that small-scale habitat fragmentations led to genetic differentiations between Myodes populations. [less ▲]

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See detailGenome-wide single nucleotide polymorphism (SNP) identification and characterization in a non-model organism, the African buffalo (Syncerus caffer), using next generation sequencing
smitz, nathalie; Van Hooft, pim; Heller, Rasmus et al

in Mammalian Biology (2016), 81

This study aimed to develop a set of SNP markers with high resolution and accuracy within the African buffalo. Such a set can be used, among others, to depict subtle population genetic structure for a ... [more ▼]

This study aimed to develop a set of SNP markers with high resolution and accuracy within the African buffalo. Such a set can be used, among others, to depict subtle population genetic structure for a better understanding of buffalo population dynamics. In total, 18.5 million DNA sequences of 76 bp were generated by next generation sequencing on an Illumina Genome Analyzer II from a reduced representation library using DNA from a panel of 13 African buffalo representative of the four subspecies. We identified 2534 SNPs with high confidence within the panel by aligning the short sequences to the cattle genome (Bos taurus). The average sequencing depth of the complete aligned set of reads was estimated at 5x, and at 13x when only considering the final set of putative SNPs that passed the filtering criterion. Our set of SNPs was validated by PCR amplification and Sanger sequencing of 15 SNPs. Of these 15 SNPs, 14 amplified successfully and 13 were shown to be polymorphic (success rate: 87%). The fidelity of the identified set of SNPs and potential future applications are finally discussed. [less ▲]

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See detailPost-glacial colonization of Europe by the wood mouse, Apodemus sylvaticus: evidence of a northern refugium and dispersal with humans
Herman, Jeremy; Johannesdottir, Frija; Jones, Eleanor et al

in Biological Journal of the Linnean Society (2016)

The wood mouse Apodemus sylvaticus is an opportunistic rodent that is found throughout most of the European mainland. It is present on many islands around the margins of the continent and in northern ... [more ▼]

The wood mouse Apodemus sylvaticus is an opportunistic rodent that is found throughout most of the European mainland. It is present on many islands around the margins of the continent and in northern Africa. The species has been the subject of previous phylogeographical studies, although these have focussed on the more southerly part of its range. A substantial number of new samples, many of them from the periphery of the species’ range, contribute to an exceptional dataset comprising 981 mitochondrial cytochrome b sequences. These new data provide sufficient resolution to transform our understanding of the survival of the species through the last glaciation and its subsequent re-colonization of the continent. The deepest genetic split that we found is in agreement with previous studies and runs from the Alps to central Ukraine, although we further distinguish two separate lineages in wood mice to the north and west of this line. It is likely that this part of Europe was colonized from two refugia, putatively located in the Iberian peninsula and the Dordogne or Carpathian region. The wood mouse therefore joins the growing number of species with extant populations that appear to have survived the Last Glacial Maximum in northern refugia, rather than solely in traditionally recognized refugial locations in the southern European peninsulas. Furthermore, the existence of a northern refugium for the species was predicted in a study of mitochondrial variation in a specific parasite of the wood mouse, demonstrating the potential value of data from parasites to phylogeographical studies. Lastly, the presence of related haplotypes in widely disparate locations, often on islands or separated by substantial bodies of water, demonstrates the propensity of the wood mouse for accidental human-mediated transport [less ▲]

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See detailAn endogenous gibbon ape leukemia virus (GALV) identified in a rodent (Melomys sp.) from Indonesia
Alfano, niccolo; Michaux, Johan ULiege; Fabre, Pierre-Henri et al

in Journal of Virology (2016)

Gibbon ape leukemia virus (GALV) and koala retrovirus (KoRV) most likely originated froma cross-species transmission of an ancestral retrovirus into koalas and gibbons via one or moreintermediate as yet ... [more ▼]

Gibbon ape leukemia virus (GALV) and koala retrovirus (KoRV) most likely originated froma cross-species transmission of an ancestral retrovirus into koalas and gibbons via one or moreintermediate as yet unknown hosts. A highly similar virus to GALV has been identified in anAustralian rodent (Melomys burtoni) after extensive screening of Australian wildlife. GALV-likeviruses have also been discovered in several Southeast Asian species although screening has not beenextensive and viruses discovered to date are only distantly related to GALV. We therefore screened 26Southeast Asian rodent species for KoRV- and GALV-like sequences, using hybridization capture andhigh-throughput sequencing, in the attempt to identify potential GALV and KoRV hosts. Only onespecies, an undescribed species of Melomys from Indonesia, was positive yielding an endogenousprovirus very closely related to a strain of GALV. The sequence of the critical receptor domain forGALV infection in the Indonesian Melomys sp. was consistent with the susceptibility of the species toGALV infection. The discovery of a GALV in a second Melomys species provides further evidencethat Melomys may play a role in the spread of GALV-like viruses, especially since the genus is foundin Indonesia, Papua New Guinea and Australia, connecting the home ranges of koalas and gibbons [less ▲]

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See detailAn endogenous gibbon ape leukemia virus (GALV) identified in a rodent (Melomys sp.) from Indonesia
Niccolo, Alfano; Michaux, Johan ULiege; Fabre, pierre-Henri et al

in Journal of Virology (2016), sous presse

ABSTRACT Gibbon ape leukemia virus (GALV) and koala retrovirus (KoRV) most likely originated from a cross-species transmission of an ancestral retrovirus into koalas and gibbons via one or more ... [more ▼]

ABSTRACT Gibbon ape leukemia virus (GALV) and koala retrovirus (KoRV) most likely originated from a cross-species transmission of an ancestral retrovirus into koalas and gibbons via one or more intermediate as yet unknown hosts. A highly similar virus to GALV has been identified in an Australian rodent (Melomys burtoni) after extensive screening of Australian wildlife. GALV-like viruses have also been discovered in several Southeast Asian species although screening has not been extensive and viruses discovered to date are only distantly related to GALV. We therefore screened 26 Southeast Asian rodent species for KoRV- and GALV-like sequences, using hybridization capture and high-throughput sequencing, in the attempt to identify potential GALV and KoRV hosts. Only one species, an undescribed species of Melomys from Indonesia, was positive yielding an endogenous provirus very closely related to a strain of GALV. The sequence of the critical receptor domain for GALV infection in the Indonesian Melomys sp. was consistent with the susceptibility of the species to GALV infection. The discovery of a GALV in a second Melomys species provides further evidence that Melomys may play a role in the spread of GALV-like viruses, especially since the genus is found in Indonesia, Papua New Guinea and Australia, connecting the home ranges of koalas and gibbons. IMPORTANCE The gibbon ape leukemia virus (GALV) and the koala retrovirus (KoRV) are very closely related, yet their hosts are neither closely related nor overlap geographically. Direct cross-species infection between koalas and gibbons is unlikely. Therfore, GALV and KoRV may have arisen via a cross-species transfer from an intermediate host that overlaps in range with both gibbons and koalas. Using hybridization capture and high-throughput sequencing, we have screened a wide range of rodent candidate hosts from Southeast Asia for KoRV- and GALV-like sequences. Only a Melomys species from Indonesia was positive for GALV. We report the genome sequence of this newly identified GALV, the critical domain for infection of its potential cellular receptor and its phylogenetic relationships with the other previously characterized GALVs. We hypothesize that the genus Melomys may have played a key role in cross-species transmission to other taxa. [less ▲]

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See detailFrom sample hunt to sequence processing, the journey of a biologist
André, Adrien ULiege; Millien, Virginie; Michaux, Johan ULiege

Conference (2015, December 08)

Metabarcoding studies are becoming more and more popular with a field of applications constantly increasing. However, the methods used are sometimes complex and might remain “obscure” for most of us. The ... [more ▼]

Metabarcoding studies are becoming more and more popular with a field of applications constantly increasing. However, the methods used are sometimes complex and might remain “obscure” for most of us. The objective of this presentation is therefore to familiarize people with this field of research by giving an overview of the different steps permitting the achievement of metabarcoding studies. Field work, lab work and bioinformatics will be subsequently detailed and accessibly explained. [less ▲]

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See detailEtude de la diversité génétique et de l’état des stocks des populations de barbeaux et de hotus en Wallonie. Amélioration des techniques d’élevage en vue de repeuplements raisonnés et de transferts de connaissances vers les pisciculteurs
Gennotte, Vincent ULiege; Prignon, Christian ULiege; Dierckx, Arnaud ULiege et al

Report (2015)

Nase (Chondrostoma nasus) and common barbel (Barbus barbus) are two rheophilic cyprinid fish naturally present in South Belgian rivers. During the last decades, the construction of dams together with ... [more ▼]

Nase (Chondrostoma nasus) and common barbel (Barbus barbus) are two rheophilic cyprinid fish naturally present in South Belgian rivers. During the last decades, the construction of dams together with changes in hydrological regimes, modifications of riverbed morphology and water pollution caused some local dramatic declines in their populations. However, recent improvements in terms of water quality and habitat fragmentation allow now to implement a rational restocking plan of locally endangered patrimonial fish species such as nase and common barbel. To reach this goal, this project (co-funded by the European Fisheries Fund and the Wallonia Public Service) proposed to develop five complementary parts with specific objectives: • Review of the knowledge on nase and barbel geographical distribution and stock health in Wallonia. This section presents the distribution and recent evolution of populations in Europe, and more specifically in Wallonia. Areas where population declines were reported are identified. Even if a weak population expansion was reported in some isolated cases, the global status of Walloon populations is still concerning. • Characterization of genetic structure and diversity of South Belgium populations. Restocking operations for a conservation purpose have to be based on the knowledge and the use of wild type genetic strains. Nase and barbel populations from South Belgium were genetically characterized by use of microsatellites. Globally, nase and barbel populations are structured on a basin scale. A slight genetic differentiation exists between populations from the Rhine basin and the Meuse basin, defining two conservation units, but no finer structure was observed among populations from the Meuse basin. Genetic variation was high within populations. Genetic structure of barbel populations is more complex due to past restocking operations with different genetic lineages. An analysis of mDNA identified 6 different haplotypes but was unable to categorize them as autochtone or allochtone. • Development of fish production techniques. The complete control of fish farming is necessary to produce high quality juveniles for restocking. All the steps of the production cycle were addressed: broodstock management and reproduction, egg incubation, larval rearing and grow-out. Production systems ranging from extensive pond culture to intensive RAS were tested and the optimal farming conditions were identified (temperature, density, feeding, tank volumes, …). • Adaptation assessment of farmed fingerlings to natural conditions. Growth and survival performances of captive farmed fish were assessed in an experimental environment that mimics natural conditions. The results suggested that the more efficient practice for restocking would be based on operations performed in spring with large juveniles (3 to 50 g). • Know-how diffusion toward fish farmers. All the breeding and grow-out techniques developed for nase and barbel production are the subject of two handbooks. These documents, attached to the report, will be published and distributed to fish producers. [less ▲]

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See detailPast and future distributions of Southeast Asian murine rodents: the influence of climate changes
Latinne, Alice ULiege; Meynard, Christine; Morand, Serge et al

Conference (2015, August 18)

Our study, involving species distribution modelling techniques, aimed at assessing the influence of past and future climatic fluctuations on Southeast Asian small mammal distributions using two forest ... [more ▼]

Our study, involving species distribution modelling techniques, aimed at assessing the influence of past and future climatic fluctuations on Southeast Asian small mammal distributions using two forest-dwelling (Leopoldamys herberti and Leopoldamys sabanus) and one karst endemic (Leopoldamys neilli) rodent species as models. Our model predictions contradict the well-established hypothesis that Southeast Asian forest-dwelling species were confined to small refugia during the LGM. Moreover, these results suggest that the distribution of several East and Southeast Asian taxa were in their refugial state during Pleistocene interglacial periods rather than during glacial periods. This could be because of vegetation changes that may have occurred at that time as a result of the increased seasonality observed during the LIG. The two future climate change scenarios used in this study predicted that large climatically suitable areas would still be available in the future for the three species. [less ▲]

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See detailEvidence of a fine-scale genetic structure for the endangered Pyrenean desman (Galemys pyrenaicus) in the French Pyrenees
Gillet, François ULiege; Cabria Garrido, Maria Teresa; Blanc, Frédéric et al

Poster (2015, August 05)

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