  Bovine herpesvirus 5 infection; Thiry, Julien  ; Thiry, Etienne in Lefèvre, P. C.; Blancou, J.; Chermette, R. (Eds.) et al Infectious and Parasitic Diseases of Livestock (2010) Detailed reference viewed: 5 (1 ULg) IBR and BVD control : the key to successful herd management; ; et al in Berliner und Munchener Tierarztliche Wochenschrift (2010), 123 Detailed reference viewed: 13 (3 ULg)Embryo transfer as a tool for experimental reproduction of ovine herds.; ; et al in Ciência Animal Brasileira (2009), Suppl 1 Detailed reference viewed: 8 (3 ULg)Bluetongue control in Europe - New challenges and achievements; ; et al in Berliner und Munchener Tierarztliche Wochenschrift (2009), 122 Detailed reference viewed: 20 (3 ULg)Vaccination of calves using the BRSV nucleocapsid protein in a DNA prime-protein boost strategy stimulates cell-mediated immunity and protects the lungs against BRSV replication and pathology.; Boxus, Mathieu ; et alin Vaccine (2008), 26(37), 4840-8 Respiratory syncytial virus (RSV) is a major cause of respiratory disease in both cattle and young children. Despite the development of vaccines against bovine (B)RSV, incomplete protection and ... [more ▼] Respiratory syncytial virus (RSV) is a major cause of respiratory disease in both cattle and young children. Despite the development of vaccines against bovine (B)RSV, incomplete protection and exacerbation of subsequent RSV disease have occurred. In order to circumvent these problems, calves were vaccinated with the nucleocapsid protein, known to be a major target of CD8(+) T cells in cattle. This was performed according to a DNA prime-protein boost strategy. The results showed that DNA vaccination primed a specific T-cell-mediated response, as indicated by both a lymphoproliferative response and IFN-gamma production. These responses were enhanced after protein boost. After challenge, mock-vaccinated calves displayed gross pneumonic lesions and viral replication in the lungs. In contrast, calves vaccinated by successive administrations of plasmid DNA and protein exhibited protection against the development of pneumonic lesions and the viral replication in the BAL fluids and the lungs. The protection correlated to the cell-mediated immunity and not to the antibody response. [less ▲] Detailed reference viewed: 46 (11 ULg)Concepts in the prevention of bovine respiratory disease.; Lekeux, Pierre ; et alin Berliner und Munchener Tierarztliche Wochenschrift (2008), 121(11-12), 446-449 The bovine respiratory disease (BRD) complex requires further research both, to fully understand the disease from the different perspectives as well as to develop new tools and strategies for vaccination ... [more ▼] The bovine respiratory disease (BRD) complex requires further research both, to fully understand the disease from the different perspectives as well as to develop new tools and strategies for vaccination and treatment was the conclusion at a recent BRD symposium in Rome, Italy. A group of scientist across Europe followed the invitation of Prof. E. Thiry (University of Liège, Belgium) to convene for a 2 days workshop type symposium sponsored by Intervet/Schering-Plough Animal Health (Fig. 1 – group picture) [less ▲] Detailed reference viewed: 56 (7 ULg)Establishment of latency by a glycoprotein E negative bovine herpesvirus type 1 vaccine: influence of viral load and effect of specific maternal antibodies; ; et al in Vaccine (2001), 19 Detailed reference viewed: 8 (2 ULg)Bovine Herpesvirus Type 1 Glycoprotein H Is Essential for Penetration and Propagation in Cell Culture; ; et al in Journal of General Virology (The) (1998), 79((Pt 8)), 1983-7 Bovine herpesvirus type 1 (BHV-1) glycoprotein H (gH) is a structural component of the virion which forms a complex with glycoprotein gL. To study the role of BHV-1 gH in the virus infectious cycle, a gH ... [more ▼] Bovine herpesvirus type 1 (BHV-1) glycoprotein H (gH) is a structural component of the virion which forms a complex with glycoprotein gL. To study the role of BHV-1 gH in the virus infectious cycle, a gH null mutant was constructed in which the gH coding sequences were deleted and replaced by the Escherichia coli lacZ cassette. The BHV-1 gH null mutant was propagated in trans-complementing MDBK cells, stably transfected with plasmid pMEP4 containing the BHV-1 gH gene under the control of the inducible mouse metallothionein promoter. Experiments with the BHV-1 gH null mutant showed that gH is essential in the infectious cycle of the virus and is specifically involved in virus entry and cell-to-cell spread. The lack of infectivity of virions devoid of gH is not due to a defect in attachment. Moreover, PEG-induced fusion of virions to target cells provides evidence that BHV-1 gH is required for virion penetration. [less ▲] Detailed reference viewed: 11 (1 ULg) |
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