References of "Mermillod, P"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailIn vitro embryo production in goats: slaughterhouse and laparoscopic ovum pick up 2 (LOPU) derived oocytes have different kinetics and requirements regarding maturation 3 media
Souza-Fabjan, JM; Locatelli, Y; Duffard, N et al

in Theriogenology (2014), 81

A total of 3427 goat oocytes were used in this study in order to identify possible 24 differences during in vitro embryo production from slaughterhouse or laparoscopic ovum 25 pick up (LOPU) oocytes. In ... [more ▼]

A total of 3427 goat oocytes were used in this study in order to identify possible 24 differences during in vitro embryo production from slaughterhouse or laparoscopic ovum 25 pick up (LOPU) oocytes. In Experiment 1, one complex, one semi defined and one simplified 26 in vitro maturation (IVM) media were compared using slaughterhouse oocytes. In 27 Experiment 2, we checked the effect of oocyte origin (slaughterhouse or LOPU) on the 28 kinetics of maturation (18 vs. 22 vs. 26 h) when submitted to semi defined or simplified 29 media. In Experiment 3, we determined the differences in embryo development between 30 slaughterhouse and LOPU oocytes when submitted to both media and then to in vitro 31 fertilization (IVF) or parthenogenetic activation (PA). Embryos from all groups were vitrified 32 and their viability evaluated in vitro after thawing. In Experiment 1, no difference (P>0.05) 33 was detected among treatments for maturation rate (MII; 88% on average), cleavage (72%), 34 blastocyst from the initial number of cumulus oocyte complexes (COC; 46%) or from the 35 cleaved ones (63%), hatching rate (69%) and the total number of blastomeres (187). In 36 Experiment 2, there was no difference of MII rate between slaughterhouse oocytes cultured 37 for 18 or 22 h, whereas the MII rate increased significantly (P<0.05) between 18 and 22 h for 38 LOPU oocytes in the simplified medium. Moreover, slaughterhouse oocytes cultured in 39 simplified medium matured significantly faster than LOPU oocytes at 18 and 22 h (P<0.05). 40 In Experiment 3, cleavage rate was significantly greater (P<0.001) in all four groups of 41 embryos produced by PA than IVF. Interestingly, PA reached similar rates for slaughterhouse 42 oocytes cultured in both media, but improved (P<0.05) cleavage rate of LOPU oocytes. 43 Slaughterhouse oocytes had acceptable cleavage rate after IVF (~67%), whereas LOPU 44 oocytes displayed a lower one (~38%), in contrast to cleavage after PA. The percentage of 45 blastocysts in relation to cleaved embryos was not affected by the origin of the oocytes 46 (P>0.05). Therefore, slaughterhouse oocytes developed a greater proportion of blastocysts than LOPU ones, expressed as the percentage of total COC entering to IVM. Vitrified-thawed 48 blastocysts presented similar survival and hatching rates between the oocyte origin, media or 49 method of activation. In conclusion, slaughterhouse and LOPU derived oocytes may have 50 different IVM kinetics and require different IVM-IVF conditions. Although the IVM and IVF 51 systems still need improvements in order to enhance embryo yield, the in vitro development 52 (IVD) step is able to generate good quality embryos from LOPU derived oocytes. [less ▲]

Detailed reference viewed: 3 (0 ULg)
Full Text
Peer Reviewed
See detailSex and PRNP genotype determination in preimplantation caprine embryos
Guignot, F.; Perreau, C.; Cavarroc, C. et al

in Reproduction in Domestic Animals (2011), 46(4), 656-663

The objective of this study was to test the accuracy of genotype diagnosis after whole amplification of DNA extracted from biopsies obtained by trimming goat embryos and to evaluate the viability of ... [more ▼]

The objective of this study was to test the accuracy of genotype diagnosis after whole amplification of DNA extracted from biopsies obtained by trimming goat embryos and to evaluate the viability of biopsied embryos after vitrification/warming and transfer. Whole genome amplification (WGA) was performed using Multiple Displacement Amplification (MDA). Sex and prion protein (PRNP) genotypes were determined. Sex diagnosis was carried out by PCR amplification of ZFX/ZFY and Y chromosome-specific sequences. Prion protein genotype determination was performed on codons 142, 154, 211, 222 and 240. Embryos were collected at day 7 after oestrus and biopsied either immediately after collection (blastocysts and expanded blastocysts) or after 24 h of in vitro culture (compacted morulae). Biopsied embryos were frozen by vitrification. Vitrified whole embryos were kept as control. DNA of biopsies was extracted and amplified using MDA. Sex diagnosis was efficient for 97.4% of biopsies and PRNP genotyping was determined in 78.7% of biopsies. After embryo transfer, no significant difference was observed in kidding rate between biopsied and vitrified control embryos, whereas embryo survival rate was different between biopsied and whole vitrified embryos (p = 0.032). At birth, 100% of diagnosed sex and 98.2% of predetermined codons were correct. Offspring PRNP profiles were in agreement with parental genotype. Whole genome amplification with MDA kit coupled with sex diagnosis and PRNP genotype predetermination are very accurate techniques to genotype goat embryos before transfer. These novel results allow us to plan selection of scrapie-resistant genotypes and kid sex before transfer of cryopreserved embryo. [less ▲]

Detailed reference viewed: 40 (3 ULg)
Full Text
Peer Reviewed
See detailIn vitro maturation treatment affects developmental competence of laparoscopic ovum pickup-derived oocytes in follicle-stimulating hormone-stimulated goats
Locatelli, Y; Poulin, N; Baril, G et al

in Reproduction, Fertility, & Development (2008), 20(1), 182-183

The aim of the present study was to assess the effect of IVM treatment on the developmental competence of oocytes recovered from repeated laparoscopic ovum pickukp (LOPU) in goats. A total of 94 LOPU ... [more ▼]

The aim of the present study was to assess the effect of IVM treatment on the developmental competence of oocytes recovered from repeated laparoscopic ovum pickukp (LOPU) in goats. A total of 94 LOPU sessions were performed on 33 adult goats of the Saanen and Alpine breeds. Females were synchronized (Day 0) during the nonbreeding season by inserting vaginal sponges (45 mg of fluorogestone acetate, Intervet, Boxmeer, The Netherlands). At Day 8, an i.m. injection of 50 μg of cloprostenol (Estrumate; Schering-Plough Animal Health, Pointe-Claire, Quebec, Canada) was administered. Porcine FSH (Stimufol, Merial, Brussels, Belgium, 160 mg/goat) was administered in 5 injections at 12-h intervals, starting on Day 8. The LOPU took place under general anesthesia on Day 11, and follicles ≥2 mm were aspirated with an 18-gauge needle connected to a controlled vacuum system. Vaginal sponges were removed at the time of LOPU. Treatments were repeated 2 times in a 2-week interval scheme (2 goats and 1 goat were excluded from the experiment during the second and third LOPU sessions, respectively). Cumulus–oocyte complexes were washed and evaluated for quality (graded from 1 to 3). Oocytes recovered from unstimulated slaughterhouse-derived ovaries served as a control. Cumulus–oocytes complexes from Grades 1 and 2 were submitted to IVM in TCM-199, supplemented with 100 μm of cysteamine and either 10 ng mL–1 of epidermal growth factor (EGF) or 10% follicular fluid and 100 ng mL–1 of ovine FSH (FF-FSH). Matured oocytes were then submitted to IVF and in vitro development as described by Cognié et al. (2004 Reprod. Fertil. Dev. 16, 437–445). Over the 94 LOPU sessions, 20.4 ± 0.9 follicles were aspirated (mean ± SEM), allowing the recovery of 12.3 ± 0.7 COC per goat and per session, of which 80.1% were suitable for IVM (Grades 1 and 2). Results of in vitro production are detailed in the table. The IVM treatment did not significantly affect cleavage or blastocyst development rates in oocytes derived from slaughterhouse ovaries. Cleavage rates were significantly decreased in LOPU-derived oocytes when compared with control oocytes. For LOPU-derived oocytes, cleavage and final blastocyst development rates were increased significantly and kinetics of embryo development were accelerated when FF-FSH was used during IVM as compared with EGF. The IVM with FF-FSH allowed us to produce 4.1 blatocysts per goat per LOPU session. These results demonstrate the interest in LOPU for goat embryo production once appropriate IVM treatment is used. The difference observed between LOPU and slaughterhouse oocytes in terms of response to IVM treatments may be related to FSH stimulation prior to the LOPU session or to postmortem changes in oocyte responsiveness in the slaughterhouse group. [less ▲]

Detailed reference viewed: 11 (1 ULg)
Full Text
Peer Reviewed
See detailImproved vitrification method allowing direct transfer of goat embryos
Guignot, F.; Bouttier, A.; Baril, G. et al

in Theriogenology (2006), 66(4), 1004-1011

The aim of this study was to design a vitrification method suited to field embryo transfer experiments in goat. In a first experiment, a standard vitrification protocol, previously designed for sheep ... [more ▼]

The aim of this study was to design a vitrification method suited to field embryo transfer experiments in goat. In a first experiment, a standard vitrification protocol, previously designed for sheep embryos was compared to slow freezing of goat embryos. No significant difference was observed on kidding rate (48% versus 69%, respectively), nor on embryo survival rate (35% versus 45%). Second experiment: all embryos were vitrified. After warming, embryos were either transferred directly (direct transfer), or after in vitro dilution of the cryoprotectants (conventional transfer). The kidding rate was not affected by the transfer method (38% versus 23%, respectively). However, embryo survival rate tended to be higher after direct transfer (26% versus 14%). Third experiment: OPS vitrification was compared to standard vitrification. The kidding rate was not affected (22% versus 39%, respectively), but the embryo survival rate was lower after OPS (14% versus 28%). Fourth experiment: 0.4 M sucrose was added with cryoprotectants in vitrification. The kidding rate after direct transfer was significantly enhanced after addition of sucrose (56% versus 27%, respectively), whereas embryo survival rate was not significantly affected (32% versus 18%). Fifth experiment: vitrification with sucrose supplementation was compared to slow freezing. No significant difference was observed after direct transfer on kidding rate (52% versus 31%, respectively), but embryo survival rate tended to be higher after vitrification (34% versus 2 1 %). In conclusion, our results indicate that addition of 0.4 M sucrose in association with direct transfer improves significantly the viability of goat vitrified embryos. [less ▲]

Detailed reference viewed: 51 (2 ULg)
Full Text
Peer Reviewed
See detailThe viability of in vitro produced sheep embryos is negatively affected by the presence of estradiol during in vitro maturation
Poulin, N.; Cognié, Y.; Baril, G. et al

in Reproduction in Domestic Animals (2005), 40(4), 92

Detailed reference viewed: 9 (2 ULg)
Full Text
Peer Reviewed
See detailEffet de prétraitements agoniste et antagoniste de GnRH sur la production d’embryons chez la brebis et la chèvre
Baril, G.; Cognié, Y.; Belloc, J. P. et al

in Proceedings: 11e Rencontres autour des Recherches sur les Ruminants (3R) (2004)

Detailed reference viewed: 15 (2 ULg)
Full Text
Peer Reviewed
See detailIn vitro survival of vitrified goat embryos: comparison of two vitrification methods
Guignot, F.; Baril, G.; Pougnard, J. L. et al

in Proceedings: 17e Réunion A.E.T.E. (2001)

Detailed reference viewed: 9 (1 ULg)
Full Text
Peer Reviewed
See detailEmbryo survival after transfer of in vitro and in vivo produced goat embryos
Cognié, Yves; Poulin, N.; Guignot, F. et al

in Proceedings: 17th Annual Meeting AETE (2001)

Detailed reference viewed: 21 (0 ULg)
Full Text
Peer Reviewed
See detailAmélioration des méthodes de cryopréservation et de transfert d’embryons chez les petits ruminants
Baril, Gérard; Cognié, Yves; Pougnard, J. L. et al

in Proceedings: 8e Rencontres autour des Recherches sur les Ruminants (2001)

ln small ruminants, the costs of embryo transfer is a main limiting factor to the use of this method. The use of ultra rapid techniques such as embryo vitrification and direct transfer may contribute to ... [more ▼]

ln small ruminants, the costs of embryo transfer is a main limiting factor to the use of this method. The use of ultra rapid techniques such as embryo vitrification and direct transfer may contribute to reduce a part of the costs and increase the use of embryo transfer in sheep and goats. ln order to evaluate the efficiency of these techniques, two experiments were performed. ln a first experiment the viability of vitrified/thawed embryos was compared to results obtained after transfer of fresh embryos in ewes or frozen embryos (with slow freezing method) in goats. The pregnancy raté at term as weIl as embryo survival rate did not differ significantly according to embryo treatment (in ewes : 72% and 60% for fresh embryos vs 72% and 50% for vitrified embryos; in goats : 69% and 55% for frozen embryos vs 48% and 39 % for vitrified embryos). ln a second experiment, the possibility to transfer the vitrified embryos or frozen embryos directly after thawing (without cryoprotectant removal and evaluation of the morphological status of the embryos) was tested by comparison with the standard technique of transfer of vitrified or frozen/thawed embryos (removal of cryoprotectant and morphological evaluation). No significant effect of the transfer method was observed on the pregnancy rate at term and embryo survival rate (in ewes/vitrified embryos : 67% and 49% for traditional transfer vs 75% and 53% for direct transfer ; in goats/ vitrified embryos : 23% and 15% for traditional transfer vs 38% and 26% for direct transfer ; in goats/frozen embryos : 74% and 45% for traditional transfer vs 71% and 57% for direct transfer) [less ▲]

Detailed reference viewed: 119 (1 ULg)
Full Text
Peer Reviewed
See detailEffet de prétraitements agoniste et antagoniste de GnRH sur la productîon d'embryons chez la brebis et la chèvre
Baril, Gérard; Cognié, Yves; Belloc, J. P. et al

in Proceedings: 8e Rencontres autour des Recherches sur les Ruminants (2001)

ln the superovulated ewe, previous studies have shown that GnRH agonist and antagonist pre-treatment improve embryo production. Consequently, an experiment on this subject was continued in order to ... [more ▼]

ln the superovulated ewe, previous studies have shown that GnRH agonist and antagonist pre-treatment improve embryo production. Consequently, an experiment on this subject was continued in order to simplify GnRH antagonist pretreatment in the ewe, and evaluate the efficiency of this technique in the superovulated goal. For the simplification of the pre-treatment, we compared the efficiency of the multiple low-dose (11' O.5mg/ day) antagonist regimen with a regimen of three injections of 1.5, 0.5, and O.5mgat 5 day intervals. The three injections regimen allowed a high ovulatory response, but a lower yield of transferable embryos (12 vs 8.3 ; P=O.08). GnRH agonist (Decapeptyl) and antagonist (Antarelix) pre-treatments were evaluated in superovulated goats. ln GnRH agonist treated goats (Decapeptyl1.8mg 22days before FSH) follicle number >3 mm was decreased (5.5 before vs 1.1 after treatment ; P<O.Ol) without an effect on the number of small follicles (2-3mm) and ovulatory response. Percentages of recovered and fertilised ova were significantly lower after Decapeptyl pre-treatment than without pre-treatment. Consequently a low number of transferable embryos per Decapeptyl treated goat was obtained (Decapeptyl + FSH: 2.2 vs FSH 4.7 ; P>O.l0). ln GnRH antagonist treated goats (Antarelix Il' 0.5mg / day before FSH) a decrease in follicle number >5 mm was observed as weIl as an increase in small follicle number (2-3mm) and ovulatory response after FSH treatment. However, this beneficial effect on ovulatory response was cancelled by an increase in the percentage of unfertilised ova and degenerated embryos. Consequently, in Antarelix treated goats the yield of embryo production was reduced (Antarelix +FSH 2.3 vs FSH 8.1 ; P<O.Ol). After in vitro fertilisation, the cleavage rate was also lower after Antarelix pre-treatment compared to the control group (84% vs 94%; P<O.Ol). However, this percentage was high (84%) as compared to the in vivo fertilisation rate (29%).A negative effect of Antarelix pre-treament on goat oocytequality is possible; nevertheless, transport and survival of spermatozoa in the genital tract are most probably affected in these conditions [less ▲]

Detailed reference viewed: 172 (1 ULg)