The mechanosensitivity of cells in joint tissues: Role in the pathogenesis of joint diseases

in Kamkin, André; Kiseleva, Irina (Eds.) Mechanosensitivity and Mechanotransduction (2011)

Synovium angiogenesis in osteoarthritis: a new therapy target for chondroitin sulfate

Poster (2010, September)

Osteoarthritis (OA) is an important cause of pain and disability in the ageing population. Angiogenesis and inflammation are closely integrated process in OA and may contribute to its pathogenesis, as ... [more ▼]

Osteoarthritis (OA) is an important cause of pain and disability in the ageing population. Angiogenesis and inflammation are closely integrated process in OA and may contribute to its pathogenesis, as well as, affect disease progression and pain. Chondroitin sulfate (CS) is a symptomatic slow acting drug for OA and there is strong evidence suggesting that CS may also be a structure disease modifying osteoarthritis drug. The mechanisms underlying these effects remain poorly understood. This work aimed to demonstrate the relation between inflammation and angiogenesis of synovium and to study the effect of CS on synovium angiogenesis. [less ▲]

La chondroitine sulfate stimule la production des facteurs anti angiogéniques TSP-1 et VEGI par les synoviocytes arthrosiques : un nouveau mécanisme d’action pour cette molécule.

in Revue du Rhumatisme (2010), 77

La chondroïtine sulfate (CS), la glucosamine sulfate (GS), la glucosamine HCl (GH) et l’acide hyaluronique (AH) sont des anti-arthrosiques symptomatiques d’action lente (AASAL) dont l’utilisation est ... [more ▼]

La chondroïtine sulfate (CS), la glucosamine sulfate (GS), la glucosamine HCl (GH) et l’acide hyaluronique (AH) sont des anti-arthrosiques symptomatiques d’action lente (AASAL) dont l’utilisation est recommandée dans le traitement de l’arthrose. Les mécanismes d’action de ces molécules demeurent mal compris. Notre étude a comme objectif d’étudier les effets de ces molécules sur la production de médiateurs impliqués dans l’angiogenèse de la membrane synoviale (MS) arthrosique [less ▲]

Chondroitin sulfate in the treatment of osteoarthritis: from in vitro studies to clinical recommendations

in Therapeutic Advances in Musculoskeletal Disease (2010), 2(6), 335-348

Curcumin inhibits pro-inflammatory mediators and metalloproteinase-3 production by chondrocytes

in Inflammation research (2009), 58

Objective and Design. This study aims to investigate the effects of curcumin (Cur) on the production of inflammatory mediators and on the extracellular matrix proteins metabolism by articular chondrocytes ... [more ▼]

Objective and Design. This study aims to investigate the effects of curcumin (Cur) on the production of inflammatory mediators and on the extracellular matrix proteins metabolism by articular chondrocytes. Methods. Human chondrocytes in alginate beads and human cartilage explants were cultured in the absence or in the presence of interleukin (IL)-1beta(10-11 M) and with or without Cur (5 to 20 µM). Nitric oxide (NO) synthesis was measured by Griess spectrophotometric method, prostaglandin (PG) E2 by a specific radioimmunoassay and IL-6, IL-8, aggrecan (Agg), matrix metalloproteinase (MMP)-3 and tissue inhibitor of metalloproteinase (TIMP)-1 by specific enzyme-amplified immunoassays. Proteoglycans degradation was evaluated by the release of 35S-glycosaminoglycans (GAG) from human cartilage explants. Results. In alginate beads and cartilage explants models, Cur inhibited in a concentration dependent manner the basal and the IL-1beta stimulated NO, PGE2, IL-6, IL-8 and MMP-3 production by human chondrocytes. The TIMP-1 and the Agg productions were not modified. In basal condition, 35S-GAG release from cartilage explants was decreased by Cur. Conclusions. Cur was a potent inhibitor of the production of inflammatory and catabolic mediators by chondrocytes, suggesting that this natural compound could be efficient in the treatment of osteoarthritis. [less ▲]

Interleukin-1beta and Interleukin-6 Disturb the Antioxidant Enzyme System in Bovine Chondrocytes: A Possible Explanation for Oxidative Stress Generation

in Osteoarthritis and Cartilage (2008), 16

OBJECTIVE: Beside matrix metalloproteinases, reactive oxygen species (ROS) are the main biochemical factors of cartilage degradation. To prevent ROS toxicity, chondrocytes possess a well-coordinated ... [more ▼]

OBJECTIVE: Beside matrix metalloproteinases, reactive oxygen species (ROS) are the main biochemical factors of cartilage degradation. To prevent ROS toxicity, chondrocytes possess a well-coordinated enzymatic antioxidant system formed principally by superoxide dismutases (SODs), catalase (CAT) and glutathione peroxidase (GPX). This work was designed to assess the effects of interleukin (IL)-1beta and IL-6 on the enzymatic activity and gene expression of SODs, CAT and GPX in bovine chondrocytes. METHODS: Bovine chondrocytes were cultured in monolayer for 4-96h in the absence or in the presence of IL-1beta (0.018-1.8ng/ml) or IL-6 (10-100ng/ml). To study signal transduction pathway, inhibitors of mitogen-activated protein kinases (MAPK) (PD98059, SB203580 and SP600125) (5-20muM) and nuclear factor (NF)-kappaB inhibitors [BAY11-7082 (1-10muM) and MG132 (0.1-10muM)] were used. SODs, CAT and GPX enzymatic activities were evaluated in cellular extract by using colorimetric enzymatic assays. Mn SODs, Cu/Zn SOD, extracellular SOD (EC SOD), CAT and GPX gene expressions were quantified by real-time and quantitative polymerase chain reaction (PCR). RESULTS: Mn SOD and GPX activities were dose and time-dependently increased by IL-1beta. In parallel, IL-1beta markedly enhanced Mn SOD and GPX gene expressions, but decreased Cu/Zn SOD, EC SOD and CAT gene expressions. Induction of SOD enzymatic activity and Mn SOD mRNA expression were inhibited by NF-kappaB inhibitors but not by MAPK inhibitors. IL-6 effects were similar but weaker than those of IL-1beta. CONCLUSIONS: In conclusion, IL-1beta, and to a lesser extend IL-6, dysregulates enzymatic antioxidant defenses in chondrocyte. These changes could lead to a transient accumulation of H(2)O(2) in mitochondria, and consequently to mitochondria damage. These changes contribute to explain the mitochondrial dysfunction observed in osteoarthritis chondrocytes. [less ▲]

L’application de forces cycliques d’étirement diminue la production de médiateurs de l’inflammation par les chondrocytes arthrosiques

in Revue du Rhumatisme (2008), 75(10-11), 999

Curcumin inhibits interleukin-6, -8, nitric oxide and prostaglandin E2 synthesis by bovine chondrocytes

Poster (2007)

La curcumine inhibe la synthèse d’ interleukine-6, -8, du monoxide d’azote et de la prostaglandine E2 par les chondrocytes bovins

in Revue du Rhumatisme (2007), 74(10-11), 1023

Interleukin-1beta and interleukin-6 disturb the antioxidant enzyme system in bovine chondrocytes: a possible explanation for oxidative stress generation

in Osteoarthritis and Cartilage (2006), 14(Suppl B), 80