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See detailStudy of the volatile organic compounds in the interaction between banana plants and the fungal disease Fusarium oxysporum f.sp. cubense Tropical Race 4 (FocTR4)
Berhal, Chadi ULg; De Clerck, Caroline ULg; LEVICEK, CAROLINA et al

Conference (2016, October 11)

Banana fruit (Musa sp.) ranks fourth in term of worldwide production, and has economical and nutritional key values. Cavendish is the popular and most dominant variety of the dessert banana group ... [more ▼]

Banana fruit (Musa sp.) ranks fourth in term of worldwide production, and has economical and nutritional key values. Cavendish is the popular and most dominant variety of the dessert banana group. Nowadays, it is endangered by the emergent race of the Panama disease, caused by Fusarium oxysporum f.sp. cubense TR4 (Foc TR4). Despite the importance of the Volatile Organic Compounds (VOCs), which are secondary metabolites with a high vapour pressure, and their role in plant protection, they have never been documented as a way to manage this disease on Cavendish. Thus, the aim of this PhD thesis is to study the VOCs in the specific interaction Cavendish/Foc TR4, as a way to manage this threat. Based on the work previously done with other plants at Gembloux Agro-Bio Tech, a protocol was optimized for the extraction of the banana plant’s VOCs. In parallel, models of in-vitro and in-vivo inoculations are under development to distinguish the VOCs emitted by the root zone and those emitted by the aerial part of the plant. The results of the first analysis showed that the majority of the Cavendish VOCs belongs to the terpenes group, as well as ketones and an organic ester. The VOCs specifically emitted during the plant-pathogen interaction will be studied more in depth by evaluating their effect on the plant and the pathogen. At long term, and depending on the results, agro-ecological applications of this knowledge could include breeding for new variety or combining banana with other plants expressing VOCs against Foc TR4, applying VOCs on field to stimulate plant defences or to contain the pathogen. [less ▲]

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See detailImpact of high-wheat bran diet on sows’ microbiota, performances and progeny’s growth and health
Leblois, Julie ULg; Bindelle, Jérôme ULg; Dehareng, Frédéric et al

Conference (2016, September 02)

Finding alternatives to antimicrobial growth promoters is part of the goal of improving sustainability in pig production. Dietary fibres are considered as health-promoting substances acting on pigs’ ... [more ▼]

Finding alternatives to antimicrobial growth promoters is part of the goal of improving sustainability in pig production. Dietary fibres are considered as health-promoting substances acting on pigs’ microbiota. This study aimed to investigate whether the enrichment of sows’ diet with high levels of wheat bran (WB) could impact the performances of sows and piglets’ health. Seven sows were fed a control diet (CON) and 8 sows a WB diet from day 43 of gestation (WB 240 g/kg DM) until the end of the lactation period (WB 140 g/kg DM). Diets were formulated to be iso-energetic and iso-nitrogenous by changing the proportions of some ingredients. Faeces were sampled at different time points (before treatment, during treatment: in gestation and lactation) to determine microbiota composition (sequencing with Illumina MiSeq). Milk was sampled weekly to determine lactose, fat and protein concentration by mid-infrared technology and IgA and IgG contents by ELISA. Before weaning (d26-27), piglets were euthanized, intestinal contents and tissues sampled for further analyses. Zootechnical performances of sows and piglets were recorded. Statistical analyses were performed using the SAS MIXED procedure and repeated measurements. Treatment never impacted piglets’ weight (P=0.51). Sows’ ingestion during the lactation period was comparable between both treatments until the last 4 days of lactation where the percentage of target ingestion was significantly (P<0.001) lower for the WB (66%) compared to the CON group (89%). No effect on sows’ backfat and weight changes was observed. An increased abundance of Lactobacillus spp. in feces of the WB group was observed in gestation before and after diet change (8.8% vs 15.1% of total bacteria). However, for the overall genera changes between treatments, it only seems to occur for minor groups of bacteria. Milk protein, fat, IgG and IgA were not affected by treatment, but a time-effect (P<0.001) was observed while treatment impacted (P<0.05) lactose content. In conclusion, sows’ performances were not affected by the high WB diet and more research on the piglets’ samples is foreseen. [less ▲]

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See detailPresentation of germplasm Health Unit-Gembloux
Massart, Sébastien ULg

Conference (2016, September)

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See detailEpidemiology and Molecular Variability of little cherry virus in Belgium
Tahzima, Rachid; Peusens, Gertie; Belïen, Tim et al

Conference (2016, September)

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See detailCOST Action FA1407: Empowering NGS technologie for study and diagnostic of plant viruses
Massart, Sébastien ULg; Petter, François

Conference (2016, July)

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See detailSerious Game Biotechnologie: dans la peau d'un responsable de laboratoire de diagnostic
Massart, Sébastien ULg

Scientific conference (2016, June 17)

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See detailTERRA Innovation Fair: Thematic 4: Innovations in PLant Protection
Massart, Sébastien ULg

Conference (2016, May 20)

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See detailInsights gained from metagenomic sequencing of apple fruit surface (cv. Pinova)
Angeli, Dario; Massart, Sébastien ULg; Sare, Abdoul Razack ULg et al

Poster (2016, May 17)

Plant microbial communities (microbiota) living at the surface of fruit have been the source of the majority of biocontrol agents (BCAs). Despite this interest, their role as a community has been poorly ... [more ▼]

Plant microbial communities (microbiota) living at the surface of fruit have been the source of the majority of biocontrol agents (BCAs). Despite this interest, their role as a community has been poorly studied so far. They holistic study was an unattainable objective due to the absence of techniques to survey microbial communities and their evolution. The recent developments in high-throughput sequencing (HTS) have now enabled the holistic analysis of the microbiota genomes. A pioneering assay has been carried out to get insight into the microbiota of apple surface through metagenome sequencing. Apple from Pinova cultivar have been sampled in orchard and the microbiota isolated. After DNA extraction, the HTS assay generated 14.5 Gbases which were assembled in 133,888 contigs. These contigs provided useful information on taxonomic composition of the microbiota. A total of 1398 bacterial species and 397 fungal species have been identified. Among them, 22 species corresponded to known apple pathogens (Penicillium expansum, Penicillium spp, Botrytis cinerea, Monilinia fructicola, Nectria haematococca, Glomerella cingulate, G. graminicola, Alternaria alternate, Alternaria brassicicola, A. citri, A. longipes, A. solani, A. tenuissima, Fusarium solani, F. oxysporum, F. culmorum, F. lateritium, Mucor circinelloides, M. hiemalis, F. racemosus, Venturia inaequalis, Cladosporium cladosporioides). In addition, several contigs were assigned to species or genus of known BCA strains like Pichia canadensis, Bacillus sp., Debaryomyces hansenii, Aureobasidium pullulans and Pantoea vagans. These results will be discussed. In any case, these results underline a very diverse microbial community whose role needs to be characterized. In this line, the functional analysis (gene function determination) of the contigs is ongoing. [less ▲]

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See detailCharacterization and biocontrol properties of Lactuca sativa rhizosphere microbiota in an aquaponic system
Stouvenakers, Gilles ULg; Massart, Sébastien ULg; Jijakli, Haissam ULg

Poster (2016, May 17)

Aquaponics is an integrated recirculated system that combines aquaculture and hydroponic plant production. The dissolved nutrients generated by the fish rearing after bacterial activities are used by the ... [more ▼]

Aquaponics is an integrated recirculated system that combines aquaculture and hydroponic plant production. The dissolved nutrients generated by the fish rearing after bacterial activities are used by the plants for their growth. This uptake reduces the accumulation of some molecules which allow a longer water recirculation. When comparing growth conditions between aquaponics and hydroponics, we observed much lower concentration of several key nutrients in aquaponics. Nevertheless, several experimentations report similar or better yield in aquaponics compared with hydroponics. One explanation of this phenomenon could be linked to microbial actions. Nowadays, microorganisms related to aquaculture and hydroponics are well characterized but very little is known about the microbiota associated with aquaponic system. The first aim of this study is to characterize the rhizosphere microbiota (metagenomics) of lettuces growing in an aquaponics system. A NGS approach targeting the 16S rDNA will be carried out to evaluate the bacterial composition and diversity. The second aim is to assess the resistance of aquaponics lettuces following a pest introduction. Yields, symptoms and microbial changes due to the pest will be recorded and analysed to determine if aquaponics systems provide a better plant protection than hydroponics. Depending on the results, the final steps will be to identify the optimal conditions for the management of the potential beneficial microbiota or isolate a beneficial microorganism. [less ▲]

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See detailA "external" StandPoint on QMS
Massart, Sébastien ULg

Conference (2016, March)

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See detailVolatile Organic Compounds:a new tool to control barley pathogens?
Kaddes, Amine ULg; Massart, Sébastien ULg; Fauconnier, Marie-Laure ULg et al

Poster (2016, February 05)

Barley is threatened by various edaphic fungal diseases. In particular, common root rot caused by Fusarium culmorum and Cochliobolus sativus is causing between 9 and 23 % of yield losses. Today, since ... [more ▼]

Barley is threatened by various edaphic fungal diseases. In particular, common root rot caused by Fusarium culmorum and Cochliobolus sativus is causing between 9 and 23 % of yield losses. Today, since most of the chemicals used for crop protection are being forbidden, there is a growing need of sustainable ways to control these diseases. A recent study has shown that barley roots infected by common root rot emitted 23 Volatile Organic Compounds (VOCs) that were not emitted by healthy barley roots. In addition it was shown that the blend of these molecules negatively impacted the growth of the two fungi. The main objective of the present study was to determine which VOCs of the blend were the most efficient in this growth reduction, and to understand their mode of action. We have found that methyl propionate (MP) and methyl acrylate (MA) reduced significantly the development of the two fungi in vitro. We also observed an inhibition of the spores’ germination in the presence of the two esters. The effect of the two molecules on infected barley seeds was also tested. We have observed that the plants coming from the seeds in contact with the VOCs did not develop symptoms of the disease. The antifungal and antibacterial activity of these VOCs on a wider range of fungal and bacterial diseases was also tested in vitro with success The two esters that we have studied could thus be an interesting starting point in the development of a sustainable way to control barley’s diseases. [less ▲]

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See detailEarly life programming of pigs' intestinal microbiota, intestinal functioning and hepatic metabolism by maternal wheat bran supplementation
Leblois, Julie ULg; Bindelle, Jérôme ULg; Dehareng, Frédéric et al

Poster (2016, February 05)

The gastrointestinal (GI) microbiota plays many roles on the host’s health, acting as a barrier against pathogens and influencing the development and maturation of the mucosa, important for host’s ... [more ▼]

The gastrointestinal (GI) microbiota plays many roles on the host’s health, acting as a barrier against pathogens and influencing the development and maturation of the mucosa, important for host’s immunity. Microbial colonization occurs pre- and postnatally, via maternal transfer i.e. by milk and by the contact with sows faeces. Hence, the early establishment of a beneficial gastrointestinal microbiota in piglets was investigated by supplementing the sows with wheat bran that we consider as a prebiotic (rich in non-starch polysaccharides). Sows were fed either a wheat bran-enriched diet (25% in gestation, 14% in lactation) either a control diet. Piglets were suckling during 4 weeks, receiving a standard creep feed containing no pre- or probiotic from the second week until weaning. The direct effect of wheat bran on the fecal microbial composition of the sow has been analyzed as well as the chemical composition and immunoglobulins content of the colostrum and milk. Sows and piglets growth performances have been recorded at different time points to verify that wheat bran doesn’t impair performances. At weaning, piglets have been euthanized and the impact of the maternal treatment was investigated at different levels: growth performances, ileal and colonic microbiota, intestinal physiology and immunological response and metabolism. A second animal experiment will be performed next year including a metabolic challenge by giving half of the piglets a high-energy diet post-weaning. [less ▲]

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