References of "Massart, Sébastien"
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See detailDiagnostic des viroses et séquençage haut-débit : vers un changement de paradigme ?
Massart, Sébastien ULg; Olmos, Antonio; Jijakli, Haissam ULg et al

in Virologie (in press), 5

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See detailVirus indexing and sanitation of international banana collection: insights from a 5-year collaborative effort
De Clerck, Caroline ULg; Parmenter, Kathleen; Vandenhouwe, Ines et al

Conference (2014, August 14)

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See detailHigh Throughput sequencing and biological research: Paradigm switches from genetics to ecophysiology
Massart, Sébastien ULg

Scientific conference (2014, June)

A technological revolution is ongoing in sequencing and is deeply impacting biological research. This leads to paradigm switches in traditional « sequencing-friendly » areas, like genetic or microbiology ... [more ▼]

A technological revolution is ongoing in sequencing and is deeply impacting biological research. This leads to paradigm switches in traditional « sequencing-friendly » areas, like genetic or microbiology, but also expands the usefulness of sequencing in other areas such as ecophysiology or ecology. [less ▲]

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See detailHigh throughput sequencing of aphid haemolymph reveals endosymbiont diversity
De Clerck, Caroline ULg; Leonard, Simon; Joncour, Pauline et al

Conference (2014, May 20)

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See detailBiofilter in aquaponics
Delaide, Boris ULg; Lambrechts, Paul Emile ULg; Willaert, Jean-François ULg et al

Poster (2014, February 07)

Aquaponics is a combination of aquaculture (fish farming) and hydroponics (horticulture). In this concept, the fish faeces and excreted ammonia are partially transformed into soluble nutrients which then ... [more ▼]

Aquaponics is a combination of aquaculture (fish farming) and hydroponics (horticulture). In this concept, the fish faeces and excreted ammonia are partially transformed into soluble nutrients which then are available for plants. Wastewater from aquaculture is filtrated by plants and return to the fish tank in a loop cycle. This strongly reduces the use of water and increases the economic efficiency with an additional production of plant, e.g. vegetables. From the hydroponics view, this combination allows important decreases in chemical nutrients use for plant grow. A central part of this system is the biofilter. The microorganisms that colonize it are essential to process the fish waste into soluble nutrients easily assimilated by plants. During this process, nitrifying bacteria convert free ammonia into nitrite and then nitrate. The first objective of this research will concern the identification and isolation of the main microorganism species involved by the dilution plating and metagenomics technics. The optimal conditions to process the fish waste will be determined for such microorganism. The efficiency of nitrite and nitrate production by these micro-organisms will be tested in different conditions (T°, pH, O2, water flow, support, etc..) in vitro at the lab scale and in aquaponics prototypes. [less ▲]

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See detailSreening of essential oils against rice pathogens isolated from Madagascar
Mamiharisoa Razanakoto, Léa ULg; De Clerck, Caroline ULg; Massart, Sébastien ULg et al

Poster (2014, February 07)

Four predominant isolated pathogens have been identified during survey of rice diseases in three regions in Madagascar. These pathogens were the bacteria Xanthomonas oryzae pv. oryzae and Pseudomonas ... [more ▼]

Four predominant isolated pathogens have been identified during survey of rice diseases in three regions in Madagascar. These pathogens were the bacteria Xanthomonas oryzae pv. oryzae and Pseudomonas fuscovaginae and the fungi Alternaria sp. and Curvularia lunata. These pathogens are causing significant yield reduction on rice, which is the staple food in this country. After the identification of those pathogens, the objective of this work was to identify appropriate control measures against them. Plants extracts has been traditionally used to control diseases in other plant species. Nowadays, the problem of residues of phytopharmaceutical product, reinforce the need for research on the development of natural plant extracts to control plant diseases. Madagascar has many endemic plant of interest and we have evaluated the antimicrobial properties of essential oils from Malagasy plants. The main object of this study is the in-vitro screening of essential oils to control the pathogens isolated on rice in Madagascar. Thirty nine essentials oils from Madagascar had been tested. Activity of essentials oils on Pseudomonas fuscovaginae, Alternaria sp. and Curvularia lunata had been tested using broth media and on agar medium for Xanthomonas oryzae pv. oryzae. The growth of the pathogen (bacteria and conidia) in the presence of the essential oil was evaluated and compared to a control. Nine of the 39 essentials oils showed interesting antimicrobial activity with an efficiency more than 70% against at least one bacterial pathogen. One essential oil inhibited the growth of the two pathogens tested. For fungal pathogens, three essential oils presented an efficiency of more than 70% against at least one fungal pathogen. These results confirm that some essential oils present an interesting antimicrobial activity. The in-situ confirmation on plant of this activity is currently ongoing. [less ▲]

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See detailCombination of Genomic and Proteomic Approaches to Characterize the Symbiotic Population of the Banana Aphid (Hemiptera: Aphididae)
De Clerck, Caroline ULg; Tsuchida, Tsutomu; Massart, Sébastien ULg et al

in Environmental Entomology (2014)

Aphids are known to live in symbiosis with speciÞc bacteria called endosymbionts that have positive or negative impacts on their hosts. In this study, six banana aphid (Pentalonia nigronervosa Coquerel ... [more ▼]

Aphids are known to live in symbiosis with speciÞc bacteria called endosymbionts that have positive or negative impacts on their hosts. In this study, six banana aphid (Pentalonia nigronervosa Coquerel) strains from various geographical origins (Gabon, Madagascar, and Burundi) were screened to determine their symbiotic content, using complementary genomic (16S rDNA sequencing and speciÞc polymerase chain reaction) and proteomic (two-dimensional difference gel electrophoresis coupled with protein identiÞcation by matrix assisted laser desorption-ionization time-of-ßight mass spectrometry) approaches. Despite the geographical heterogeneity, the combined methods allowed us to identify the same two symbionts in the six aphids strains tested: Buchnera aphidicola and Wolbachia. Although B. aphidicola is found in almost all aphid species, the systematic presence of Wolbachia in banana aphids is particularly interesting, as this bacterium usually has a low prevalence in aphid species. Phylogenetic analyses showed that the Wolbachia sp. strain found in P. nigronervosa was very similar to the strain present in aphids of the genus Cinara, known to have developed a strong and long-term symbiotic association with Wolbachia. The high level of asexual reproduction in P. nigronervosa could be linked to the presence of Wolbachia, but its prevalence also suggests that this symbiotic bacterium could play a more essential role in its aphid host. [less ▲]

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See detailPichia anomala and Candida oleophila in Biocontrol of Postharvest Diseases of Fruits: 20 Years of Fundamental and Practical Research
Massart, Sébastien ULg; Jijakli, Haissam ULg

in Prusky, D.; Gullino, ML. (Eds.) Plant Pathology in the 21st Century: Contributions to the 10th International Congress of Plant Pathology (2014)

The economic losses caused by post-harvest pathogens of apple and pear can still reach 25 %. There is currently an increasing demand to develop sustainable methods to control these post-harvest pathogens ... [more ▼]

The economic losses caused by post-harvest pathogens of apple and pear can still reach 25 %. There is currently an increasing demand to develop sustainable methods to control these post-harvest pathogens. Biocontrol agents are interesting candidates to answer this demand. Nevertheless, their commercial development is sometimes hampered by a low or non-reliable efficacy comparing to fungicide treatments. Fundamental research on the mode of action of the BCA and of its ecological fitness could help to overcome that phenomenon. This chapter reviews the progresses made during two decades to understand the mode of action and the ecological niche of two BCA, Pichia anomala strain K and Candida oleophila strain O. These advances required the combination of various methodologies (in vitro and in situ) and techniques (microbiology, microscopy, genome characterization, transcriptome, proteome, gene disruption. . .) which are summarized here. Importantly, the practical impact of these discoveries to improve the efficacy of the biopesticide is also highlighted. [less ▲]

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See detailCurrent impact and future directions of high throughput sequencing in plant virus diagnostics.
Massart, Sébastien ULg; Olmos, Antonio; Jijakli, Haissam ULg et al

in Virus Research (2014), 188

The ability to provide a fast, inexpensive and reliable diagnostic for any given viral infection is a key parameter in efforts to fight and control these ubiquitous pathogens. The recent developments of ... [more ▼]

The ability to provide a fast, inexpensive and reliable diagnostic for any given viral infection is a key parameter in efforts to fight and control these ubiquitous pathogens. The recent developments of highthroughput sequencing (also called Next Generation Sequencing – NGS) technologies and bioinformatics have drastically changed the research on viral pathogens. It is now raising a growing interest for virus diagnostics. This review provides a snapshot vision on the current use and impact of high throughput sequencing approaches in plant virus characterization. More specifically, this review highlights the potential of these new technologies and their interplay with current protocols in the future of molecular diagnostic of plant viruses. The current limitations that will need to be addressed for a wider adoption of high-throughput sequencing in plant virus diagnostics are thoroughly discussed. [less ▲]

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See detailINCIDENCE DES MALADIES BACTERIENNES ET FONGIQUES DU RIZ A MADAGASCAR
Mamiharisoa Razanakoto, Léa ULg; De Clerck, Caroline ULg; Massart, Sébastien ULg et al

Poster (2013, December)

Le riz, c’est à la fois la culture la plus cultivée à Madagascar mais aussi la principale base de l’alimentation des malgaches. Même si le pays est à vocation agricole, l’autosuffisance alimentaire n’a ... [more ▼]

Le riz, c’est à la fois la culture la plus cultivée à Madagascar mais aussi la principale base de l’alimentation des malgaches. Même si le pays est à vocation agricole, l’autosuffisance alimentaire n’a jamais été atteinte, et l’importation de riz est toujours importante chaque année. Pour le riz, les maladies constituent le facteur entraînant la mauvaise qualité et la réduction des récoltes. C’est la raison qui nous amène à réaliser le suivi de la maladie de riz présent à Madagascar. L’incidence de la maladie a été évalué par l’observation visuelle des symptômes au champ et l’identité des pathogènes a été confirmée par les tests au laboratoire. Pour cela un échantillonnage a été réalisé dans les régions de Vakinankaratra, Analamanga et Atsinanana. Une enquête a été réalisée durant l’échantillonnage pour récolter les informations qui peuvent nous aider à l’identification de l’agent pathogène. L’isolement a été réalisé en utilisant les milieux standards pour bactéries et pour champignons. Les isolats ont été ensuite identifiés par l’observation des colonies sur boite de Pétri, par des observations microscopique des cellules en présence de différents colorants. Elles ont ensuite été identifiées par analyse moléculaire par séquençage.Dans les régions du haut plateau : les symptômes trouvés sont très variés et l’incidence de chaque symptôme est faible sauf le flétrissement des feuilles de riz. Dans la région Atsinanana, les symptômes trouvés ne varient pas et l’incidence est proche de 100 %. L’isolement des microorganismes à partir d’échantillons de chacune de ces régions confirme les résultats obtenus : Xanthomonas oryzae pv. oryzae correspondait à l’espèce pathogène dominante pour les hauts plateaux tandis que Curvularia lunata correspondait à l’espèce pathogène dominante pourAtsinanana. Ces différences trouvent probablement leur origine dans les conditions climatiques et le mode de culture très différentes entre cette région côtière et les hauts plateaux. [less ▲]

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See detailEcotoxicology inside the gut: impact of heavy metals on the mouse microbiome
Breton, Jérome; Massart, Sébastien ULg; Vandamme, P. et al

in BMC Pharmacology (2013), 14(62),

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See detailOncogenome sequencing: is it sustainable?
Massart, Sébastien ULg; Alexandre, L.

Conference (2012, June)

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See detailassessmentof the probiotic potential of a dairy product fermented by propionibacterium freudenreichii in piglets.
Cousin, F.J.; Foligné, B.; Deutsch, S.-M. et al

in Journal of Agricultural and Food Chemistry (2012), 60(32), 7917-7927

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See detailModels to predict the combined effects of temperature and relative humidity on Pectobacterium Atrosepticum and Pectobacterium carotovorum subsp. Carotovorum population density and soft rot disease development at the surface of wounded potato tubers
Moh, Augustin; Massart, Sébastien ULg; Lepoivre, Philippe ULg et al

in Journal of Plant Pathology [=JPP] (2012), 94(1), 181-191

The main objectives of this study were to evaluate and model the influence of temperature (10, 15 and 20°C), relative humidity (86, 96 and 100%) and initial concentration of bacterial inoculum (105, 107 ... [more ▼]

The main objectives of this study were to evaluate and model the influence of temperature (10, 15 and 20°C), relative humidity (86, 96 and 100%) and initial concentration of bacterial inoculum (105, 107 et 109 CFU ml-1) on the population density of Pectobacterium atrosepticum (Pba) and Pectobacterium carotovorum subsp. carotovorum (Pcc) which are important potato pathogens in temperate climates, and on the development of soft rot symptoms caused by these bacteria at the surface of wounded potatoes tubers under controlled conditions. Experiments were carried out according to a Box-Behnken experimental design, simplifying prediction of the combined effects of three controlled factors. With both bacterial species, statistical analysis showed a significant effect of temperature, relative humidity and initially applied bacterial concentration on population dynamics and soft rot development at the surface of wounded potato tubers. Multiple regression analyses and the contour plots showed that the temperature is the most important factor, followed by the initially applied bacteria concentration and relative humidity. More than 64% of the variability of the soft rot symptoms observed could be explained by the presence of Pba and Pcc at the level of wounded potato tubers under the combined effect of tested factors. The quadratic polynomial models developed in our research should integrate the heterogeneity of tested bacteria belonging to the same species (which was not evaluated in this preliminary investigation) in further research. [less ▲]

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