References of "Martinelle, Ludovic"
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See detailOrbivirus screening on dried blood spots from captive oryx in United Arab Emirates stresses the importance of pre-import measures
Martinelle, Ludovic ULg; Haegeman, Andy; Lignereux, Louis et al

Poster (2015, September 01)

Objective: Following reintroduction and conservation programs of the Arabian oryx (Oryx leucoryx) and the scimitar horned oryx (SHO, Oryx dammah) in the United Arab Emirates (UAE), import of animals from ... [more ▼]

Objective: Following reintroduction and conservation programs of the Arabian oryx (Oryx leucoryx) and the scimitar horned oryx (SHO, Oryx dammah) in the United Arab Emirates (UAE), import of animals from wild game ranches in the United States of America (USA) is not uncommon. Bluetongue virus (BTV) and epizootic hemorrhagic disease virus (EHDV) are orbiviruses that are the causative agents of bluetongue disease (BT) and epizootic hemorrhagic disease (EHD), respectively. BTV and EHDV are endemic in the UAE and the USA. Sheep and some wild ruminant species are usually severely affected by BT whereas EHD mostly affects wild animals and sometimes cattle. The objective of this study was to estimate the prevalence of these orbiviruses in Arabian and SHO from captive herds in the UAE using serology and molecular virology. Dry blood spot sampling for orbivirus screening is also discussed. Methods: A total of 175 SHO and 16 Arabian oryx were sampled. The latters were imported from Texas (USA) two weeks before sampling. All sampled animals belonged to captive herds spread over the Al Wathba area. For biosecurity reasons and to simplify blood storage, elutes from dried blood spot were used for serological and virological tests. Drops of about 80 µl of blood were dispensed on Whatman protein saver cards, and then allowed to dry in the dark at room temperature for 48 hours. Blood spots were punched out in paper discs with a 6 mm diameter punch and diluted in 250 µl PBS and Tween 20 0.05%. Eluted samples were incubated overnight at room temperature and then used immediately or stored at -80°C. To assess the most suitable ELISA kit to detect anti-BTV antibodies from the oryx discs, similar discs were prepared using blood issued from BTV seropositive and viremic as well as seronegative and non-viremic cattle. Elutes from discs with dried-blood from cattle were tested by BTV competitive ELISA (cELISA), sandwich ELISA (sELISA) and indirect ELISA (iELISA) and compared to cELISA performed directly on the serum of the same animals. iELISA on cattle paper discs gave the best correspondence with cELISA on cattle serum and was therefore used to test the oryx paper discs. Subsequently oryx paper discs were tested to detect antibodies against EHDV by cELISA. All the paper discs elutes from Arabian oryx and ELISA positive elutes from SHO were also tested by pan-BTV RTqPCR targeting a fragment of BTV segment 5 and detecting all BTV serotypes. Serotype specific end-point RT-PCR targeting a fragment of segment 2 of BTV2, BTV8, BTV10, BTV11, BTV13 and BTV17 were performed on pan-BTV positive samples. Results: Three out of 175 SHO and eight out of 16 Arabian oryx were found BTV seropositive by iELISA. None of the animals could be found seropositive against EHDV. BTV genome was detected in 1/3 seropositive SHO and in 5/16 of the Arabian oryx, amongst those 2/5 were seronegative. Overall Cq values were high (33-39). End point PCR failed to detect positive samples for any of the tested serotypes. Conclusion: BTV seroprevalence and RNA detection in SHO was very limited. By contrast BTV could be demonstrated in 5/16 imported Arabian oryx by molecular virology and in 8/16 by serology. The sampling was realized two weeks after the animals arrived in UAE and some oryx were viremic and seronegative, possibly suggesting a recent infection. Among the local SHO a low BTV seroprevalence was observed (3/175) and no animals were found positive to EHDV. This result was quite surprising because previous studies showed a higher BTV seroprevalence in domestic and wild ruminants of the Arabian Peninsula with wide local variations. In addition, dried blood spot testing has been demonstrated being a convenient and reliable method of sampling when storage conditions are hazardous. BTV serotypes could not be determined by end-point RT-PCR. At least 15 different BTV serotypes were reported in the USA and at least 10 in the Middle East, thus the oryx could be infected by a serotype not tested so far. Since RTqPCR positive values were high, the sensitivity of end-point RT-PCR might be insufficient to detect BTV out of eluted blood spots. Additional testing will be performed to identify the virus on the serotype level and therefore provide new insights to clarify the origin of the infection of the oryx. These results stress the need for pre-import risk assessment, precaution and implementation of biosecurity measures when considering translocation of wild ruminant species susceptible to BTV and EHDV. [less ▲]

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See detailA retrospective study on Equine Herpes Virus type-1 associated myeloencephalopathy in France (2008-2011)
van Galen, G; Leblond, A; Tritz, P et al

Poster (2015)

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See detailA retrospective study on equine herpesvirus type-1 associated myeloencephalopathy in France (2008-2011)
Van Galen, G; Leblond, A; Tritz, P et al

in Veterinary Microbiology (2015)

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See detailLes porcheries : réservoirs des Culicoides (Diptera: Ceratopogonidae), vecteurs des virus de la Maladie de la Langue bleue et de Schmallenberg ?
Zimmer, Jean-Yves ULg; Saegerman, Claude ULg; Martinelle, Ludovic ULg et al

in Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2014), 18(4), 480-487

La fièvre catarrhale ovine (FCO) est une arbovirose qui affecte les ruminants domestiques et sauvages. Depuis sa récente apparition en Europe du Nord, cette épizootie virale a engendré des pertes ... [more ▼]

La fièvre catarrhale ovine (FCO) est une arbovirose qui affecte les ruminants domestiques et sauvages. Depuis sa récente apparition en Europe du Nord, cette épizootie virale a engendré des pertes économiques considérables. Les vecteurs biologiques du virus de la FCO sont des moucherons piqueurs appartenant au genre Culicoides (Diptera : Ceratopogonidae). Plusieurs campagnes de piégeage lumineux de ces moucherons adultes ont été réalisées précédemment en Belgique au sein d’exploitations bovines et ovines, mais aucune à l’intérieur des exploitations porcines. Cette étude vise donc à évaluer, au moyen de pièges lumineux, les populations de culicoïdes éventuellement présentes à l’intérieur de deux porcheries belges au cours de l’automne et de l’hiver 2008. La présence des espèces (potentiellement) vectrices du genre Culicoides a ainsi été mise en évidence à l’intérieur de ces bâtiments durant l’automne : 8 et 749 spécimens appartenant à 2 et 7 espèces ont ainsi respectivement été piégés au sein des porcheries, avec une majorité de femelles du complexe Obsoletus. L’ouverture des bâtiments semble fortement influencer leur présence. L’observation du statut alimentaire des femelles laisse supposer que ces moucherons sont susceptibles de se nourrir ou de pondre au sein des porcheries, même si le sang de porc n’a pas pu être identifié dans l’abdomen des femelles gorgées et que le lisier n’a pas révélé la présence de larves. Les porcs pourraient ainsi intervenir dans le maintien des populations d’espèces potentiellement vectrices du virus de la FCO, ou du nouveau virus dénommé virus Schmallenberg. [less ▲]

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See detailDose-dependent effect of experimental Schmallenberg virus infection in sheep
Poskin, A; Martinelle, Ludovic ULg; Mostin, L et al

in Veterinary Journal (2014), 201(3), 419-422

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See detailPreliminary survey on the impact of Schmallenberg virus on sheep flocks in south of Belgium
Saegerman, Claude ULg; Martinelle, Ludovic ULg; Dal Pozzo, Fabiana ULg et al

in Transboundary and Emerging Diseases (2014), 61(5), 469-472

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See detailField veterinary survey on clinical and economic impact of Schmallenberg virus in Belgium
Martinelle, Ludovic ULg; Dal Pozzo, Fabiana ULg; Gauthier, B et al

in Transboundary and Emerging Diseases (2014), 61(3), 285-288

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See detailClinical pattern characterisation of cattle naturally infected by BTV-8 - Clinical characterisation of BTV-8 infected cattle
Zanella, G; Martinelle, Ludovic ULg; Guyot, Hugues ULg et al

in Transboundary and Emerging Diseases (2013), 60(3), 231-237

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See detailLe virus Schmallenberg ou l’émergence du premier Orthobunyavirus du sérogroupe Simbu en Europe
Martinelle, Ludovic ULg; Dal Pozzo, Fabiana ULg; Kirschvink, N et al

in Annales de Médecine Vétérinaire (2012), 156(1), 07-24

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See detailEvidence-based early clinical detection of emerging diseases in food animals and zoonoses: two cases.
Saegerman, Claude ULg; Humblet, Marie-France ULg; Porter, Sarah ULg et al

in Veterinary Clinics of North America. Food Animal Practice (2012), 28(1), 121-131

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See detailOriginal findings associated with two cases of bovine papular stomatitis
Dal Pozzo, Fabiana ULg; Martinelle, Ludovic ULg; Gallina, L. et al

in Journal of Clinical Microbiology (2011), 49(12), 4397-4400

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See detailTwo alternative inocula to reproduce bluetongue virus serotype 8 disease in calves
Martinelle, Ludovic ULg; Dal Pozzo, Fabiana ULg; Sarradin, P. et al

in Vaccine (2011), 29

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See detailEpidémiologie de la fièvre catarrhale ovine en Europe : situation actuelle et perspectives
Saegerman, Claude ULg; Berkvens, D.; Philip, M. et al

in Epidémiologie et Santé Animale (2008), 54

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