References of "Marlier, Didier"
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See detailDetection of Usutu virus in a bullfinch (Pyrrhula pyrrhula) and a great spotted woodpecker (Dendrocopos major) in north-west Europe
Garigliany, Mutien-Marie ULg; Marlier, Didier ULg; Tenner-Racz, Klara et al

in Veterinary Journal (2014), 199

In October 2012, a 3-year-old bullfinch (Pyrrhula pyrrhula) held in captivity for its entire lifespan and a wild adult great spotted woodpecker (Dendrocopos major), both with neurological signs, were ... [more ▼]

In October 2012, a 3-year-old bullfinch (Pyrrhula pyrrhula) held in captivity for its entire lifespan and a wild adult great spotted woodpecker (Dendrocopos major), both with neurological signs, were found 4 km from each other and 5 days apart in the Meuse Valley, Belgium. Non-suppurative encephalitis and mild degeneration and necrosis were identified in the brain and cerebellum, and Usutu virus antigen and RNA were detected by immunohistochemistry and real-time reverse transcriptase PCR, respectively. The two cases reported here represent the most western distribution of clinical disease in birds due to Usutu virus. [less ▲]

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See detailDetection of Usutu virus in a bullfinch (Pyrrhula pyrrhula) and a great spotted woodpecker (Dendrocopos major) in north-west Europe
Garigliany, Mutien-Marie ULg; Marlier, Didier ULg; Tenner-Racz, Klara et al

in Veterinary Journal (2014), 199

In October 2012, a 3-year-old bullfinch (Pyrrhula pyrrhula) held in captivity for its entire lifespan and a wild adult great spotted woodpecker (Dendrocopos major), both with neurological signs, were ... [more ▼]

In October 2012, a 3-year-old bullfinch (Pyrrhula pyrrhula) held in captivity for its entire lifespan and a wild adult great spotted woodpecker (Dendrocopos major), both with neurological signs, were found 4 km from each other and 5 days apart in the Meuse Valley, Belgium. Non-suppurative encephalitis and mild degeneration and necrosis were identified in the brain and cerebellum, and Usutu virus antigen and RNA were detected by immunohistochemistry and real-time reverse transcriptase PCR, respectively. The two cases reported here represent the most western distribution of clinical disease in birds due to Usutu virus. [less ▲]

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See detailContribution à la recherche des conditions optimales pour élever des lapins en parcs hors sol : Résultats d’un centre de référence et d’expérimentation en Belgique en conditions de production
Jacquet, Michel; Bauwens, Véronique; Teller, Christian et al

in Itavi (Ed.) Proceedings 15ème journées de la recherche cunicole (2013, November)

L’évolution de la perception sociétale du bien-être animal et de l'élevage engage la production à chercher des alternatives aux cages conventionnelles. Dans cette expérimentation en engraissement en ... [more ▼]

L’évolution de la perception sociétale du bien-être animal et de l'élevage engage la production à chercher des alternatives aux cages conventionnelles. Dans cette expérimentation en engraissement en conditions de production, durant un an, les performances (techniques et statut sanitaire) ont été suivies dans un logement en parcs hors sol (bâtiment 1), sur plancher en caillebotis en plastique normalement conçu pour porcelets, enrichis avec une plate-forme surélevée, matériau à ronger et cachette. Sans effectuer une comparaison scientifique de deux systèmes (parc hors sol et cage), les auteurs situent les performances enregistrées en parcs durant 7 bandes (960 lapins par bande) vis-à-vis de celles obtenues simultanément dans le même élevage, dans un logement en cages conventionnelles et enrichies (bâtiment 2), en tout plein tout vide (7 bandes et 4200 lapins par bande). Les parcs hors sol, a priori profitables au bien-être animal, non bivalents (donc changement au sevrage) ont été défavorables à la santé des lapins et à la viabilité de la production. La coccidiose intestinale a contribué à une hausse sévère de la mortalité (13,8% contre 4,8% en cages) ; de fortes baisses de performances techniques et économiques ont été constatées et la situation a contraint à un traitement anticoccidien. [less ▲]

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See detailLa virothérapie oncolytique médiée par le virus de la myxomatose
Krygier, David; Gillet, Laurent ULg; Marlier, Didier ULg

in Annales de Médecine Vétérinaire (2013), 157(1), 5-14

Le virus de la myxomatose est un poxvirus du genre Leporipoxvirus qui induit une pathologie spécifique, la myxomatose, chez le lapin européen (Oryctolagus cuniculus). Ce virus a la particularité d’être ... [more ▼]

Le virus de la myxomatose est un poxvirus du genre Leporipoxvirus qui induit une pathologie spécifique, la myxomatose, chez le lapin européen (Oryctolagus cuniculus). Ce virus a la particularité d’être non pathogène pour les autres espèces de vertébrés y compris l’homme. Le virus de la myxomatose (MYXV) présente aussi, de manière inattendue, un tropisme pour les cellules cancéreuses humaines in vitro ainsi qu’un potentiel oncolytique in vivo. La tolérance de ces cellules au MYXV est intimement liée au niveau intracellulaire d’Akt phosphorylée. Cette enzyme, est une serine/thréonine protéine kinase qui joue un rôle essentiel dans de nombreux processus cellulaires et fait partie de la voie de signalisation PI3k/Akt/mTOR (mammalian target of rapamycin), fréquemment amplifiée par l’oncogénèse. La protéine virale à répétitions ankyrines, M-T5, interagit avec Akt ce qui module le tropisme du MYXV pour les cellules tumorales humaines. Un régulateur de la croissance cellulaire et du métabolisme situé en aval d’Akt, mTOR, est spécifiquement inhibé par la rapamycine. Ainsi, l’utilisation de la rapamycine en combinaison avec le MYXV permet d’augmenter la concentration d’Akt phosphorylée, et par conséquent, d’amplifier l’oncolyse. Un meilleur contrôle chimique de la voie de signalisation d’Akt ou de la modification génétique de son génome constituera une étape décisive pour que le MYXV devienne l’un des nouveaux traitements des cancers chez l’homme [less ▲]

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See detailPrevalence of Campylobacter spp infection in male mule ducks in Belgium
Flament, Aline; Soubbotina, Alexandra ULg; Mainil, Jacques ULg et al

in Abstracts of the XVIIIth World Veterinary Poultry Association Congress (2013, August)

Salmonella enterica and Campylobacter spp food poisoning are major public health burden. In Belgium, breeding of mule duck is well developed and “foie gras” consumption shows a yearly increase. Flament et ... [more ▼]

Salmonella enterica and Campylobacter spp food poisoning are major public health burden. In Belgium, breeding of mule duck is well developed and “foie gras” consumption shows a yearly increase. Flament et al. (2012) published a paper on the Belgian Salmonella spp infection in ducks but up to now there are no data about Campylobacter prevalence. The monitoring of Campylobacter spp infections was performed from March 2008 to April 2009 in 9 Belgian duck farms starting at the arrival of French ducklings up to the end of the force-feeding period. Cotton-tipped swabs of droppings were collected in duckling transportation boxes 24 to 48 h after the arrival of animals in the farm. Pools of bird droppings (10 to 50 g in 3 to 5 samples, representative of the housing area) were collected at 3, 6, 9 weeks of age and at time of introduction in the force-feeding rooms (at 11 or 12 weeks of age). A membrane filter method adapted from the original Steele and Mc Dermott (1984) method was used. After the filtering step, Campy blood free selective medium plates were incubated at 37°C, micro-aerobically ([O2] < à 10%) in anaerobic jars and colonies corresponding to putative Campylobacter spp were further identified with the API Campy® system. Campylobacter spp was isolated from all flocks, most of the time already at arrival of ducklings or at 3 weeks of age. Campylobacter jejuni subsp. jejuni (32/42) was the most frequent species isolated (Fig.1). Campylobacter coli (3/42), Campylobacter jejuni subsp. doylei (2/42) and Helicobacter fennelliae (3/42) were unfrequently isolated. Two isolates could not be identified to the species. Our results confirm those of Tsai et Hsiang (2005) who found a prevalence of 92% for Campylobacter in Taiwan. The isolations of Campylobacter jejuni subsp. doylei and Helicobacter fennelliae were unexpected. Indeed man is considered the reservoir of Campylobacter jejuni subsp. doyley and up to now the isolation of Helicobacter was never reported from ducks (Anas platyrhynchos) (Murphy et al., 2005). In conclusion the putative role of mule ducks in the transmission of Campylobacter food borne illness should not be underestimated [less ▲]

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See detailIN-VITRO EVALUATION OF A-5021 ANTI-VIRAL ACTIVITY AGAINST TESTUDINID HERPESVIRUS 3 AND INITIAL PHARMACOKINETIC STUDY IN HERMANN'S TORTOISE (Testudo hermanni)
Gandar, Frederic ULg; Vrancken, Robert; Diez, Marianne ULg et al

Conference (2013, April 23)

Testudinid herpesvirus infections in tortoises belonging to the Testudinidae family are well known for decades, but their pathogenesis remains poorly understood and treatments are often empirical. This ... [more ▼]

Testudinid herpesvirus infections in tortoises belonging to the Testudinidae family are well known for decades, but their pathogenesis remains poorly understood and treatments are often empirical. This study describes the in vitro evaluation of selected anti-herpesvirus compounds against Testudinid Herpesvirus 3 (THV-3). A-5021, a compound with known broad-spectrum anti-herpetic activity, showed to be 9 times more potent than acyclovir, with an EC50 of 13.2 µM and inducing a complete inhibition of viral replication at 37.7 µM. Initial pharmacokinetic parameters were determined after a single sub-cutaneous administration of 5 and 10 mg/kg in Hermann’s tortoises (Testudo hermanni, n=3). Blood samples were collected at different time points and plasma concentrations of A-5021 were determined. No adverse effects were clinically observed and plasma concentrations remained above the EC50 for 2.8 and 4.2 h after administration of 5 and 10 mg/kg, respectively. These preliminary data provide a basis for further proof-of-concept studies for a potential prophylactic or therapeutic treatment of THV-3 infection in tortoises [less ▲]

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See detailPyrosequencing of epizootic rabbit enteropathy inocula and rabbit caecal samples.
Huybens, N.; Houeix, J.; Licois, D. et al

in Veterinary Journal (2013), 196

The aetiological agent of epizootic rabbit enteropathy (ERE) is still unknown although a bacterial infection seems the most likely hypothesis. In this study, amplification of the V5 and V6 regions of ... [more ▼]

The aetiological agent of epizootic rabbit enteropathy (ERE) is still unknown although a bacterial infection seems the most likely hypothesis. In this study, amplification of the V5 and V6 regions of 16SrDNA from four virulent and two non-virulent caecal samples was performed using a pyrosequencing platform. The virulent samples did not group in the same cluster. The bacterial flora identified was both different and richer than the cultivable bacterial flora. These findings highlight the need for biomolecular techniques to identify the aetiological agent of ERE. [less ▲]

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See detailControl of Salmonella contamination of shell eggs – preharvest and postharvest methods: a review
Galis, Anca; Marcq, Christopher ULg; Marlier, Didier ULg et al

in Comprehensive Reviews in Food Science and Food Safety (2013), 12

Salmonella Enteritidis is one of the most prevalence foodborne pathogen, its most important reservoir being considered the shell egg. As the concerns related to the increasing human salmonellosis cases ... [more ▼]

Salmonella Enteritidis is one of the most prevalence foodborne pathogen, its most important reservoir being considered the shell egg. As the concerns related to the increasing human salmonellosis cases grow, the need for an application of preventive methods either at the farm level or during the processing steps is crucial for a better control of the foodborne outbreaks due to the consumption of this specific food product. This review focuses on the application of preventive methods at the farm level, on preharvest step, in order to reduce the risk of shell eggs contamination with Salmonella, especially S. Enteritidis, through a better control of the laying hens’ infection with this pathogen. As postharvest methods, a first approach is the egg storage conditions and the prevention of Salmonella spp. growth and multiplication. In addition, shell eggs may be subjected to eggshell decontamination, to reduce the risk of foodborne outbreaks. Several of these latter mentioned methods are already authorized to be put in place in different countries, as it is the case in the United States of America and Canada. Their efficacy has been proven and their use is regarded by some as mandatory for ensuring shell eggs safety for the consumers. [less ▲]

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See detailComparison of corticosterone, cortisol, triglycerides, aspartateaminotransferase and uric acid plasma concentrations during a foie gras production period in male mule ducks (Anas platyrhynchos X Cairina moschata)
Flament, Aline; Delleur, Valéry ULg; Poulipoulis, Anastassios ULg et al

in British Poultry Science (2012), 53(4), 408-414

1 Corticosterone, cortisol, triglycerides, aspartate aminotransferase (AST) and uric acid (UA) plasma concentration were measured at 8 and 12 weeks of age, at slaughtering and 45 min after an ... [more ▼]

1 Corticosterone, cortisol, triglycerides, aspartate aminotransferase (AST) and uric acid (UA) plasma concentration were measured at 8 and 12 weeks of age, at slaughtering and 45 min after an adrenocorticotrophic hormone (ACTH) stimulation test (8 weeks of age) in twelve male mule ducks. 2 No significant increase of corticosterone was found during the force feeding period. 3 Comparison of corticosterone and cortisol levels indicates that cortisol can be considered as a reliable acute stress indicator in future routine examinations. 4 Triglycerides and AST plasma concentrations progressively increased from pre-force feeding period to slaughtering. 5 UA plasma concentrations also increased from the start at 8 weeks of age to mid-force feeding time but no difference was noticed between mid-force feeding period and slaughtering. [less ▲]

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See detailContraintes techniques et sanitaires de la production traditionnelle de pintade en Afrique subsaharienne
Boko, C; Kpodekon, T; Dahouda, M et al

in Annales de Médecine Vétérinaire (2012), 156

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See detailPrevalence of Salmonella serotypes in male mule ducks in Belgium
Flament, Aline; Soubbotina, Alexandra; Mainil, Jacques ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2012)

Salmonella species infections of male mule ducks were studied for 32 months in 100 flocks on nine duck farms in Belgium. The prevalence of Salmonella species infections changed significantly over time (P ... [more ▼]

Salmonella species infections of male mule ducks were studied for 32 months in 100 flocks on nine duck farms in Belgium. The prevalence of Salmonella species infections changed significantly over time (P<0.001) with infection rates of 50, 13.4, 6.7, 2.6 and 2.9 per cent, respectively, at the time of arrival on the farm, at three, six and nine weeks of age, and when the ducks left the breeding unit to enter the force-feeding rooms (at 11 or 12 weeks of age). During the study period, 95 strains of Salmonella were isolated, belonging to 11 serotypes. S Indiana (42.1 per cent) and S Regent (36.8 per cent) were the two most common serotypes, whereas S Typhimurium and S Enteritidis were found only once (1.1 per cent). All isolated strains were resistant to at least two antimicrobials, but resistance to more than five antimicrobials was observed in 21.6 per cent of the strains. [less ▲]

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See detailPneumonia with Aeromonas sobria in a Carpet Python
Gandar, Frederic ULg; Szalo, Ioan Mihai ULg; Marlier, Didier ULg

Conference (2011, August 11)

Aeromonas sobria was isolated and identified upon post-mortem examination from the respiratory tract and the blood of a carpet python (Morelia spilota variegata). The snake was referred to the Faculty of ... [more ▼]

Aeromonas sobria was isolated and identified upon post-mortem examination from the respiratory tract and the blood of a carpet python (Morelia spilota variegata). The snake was referred to the Faculty of Veterinary Medicine of Liège for necropsy, just the day after it suddenly died without previous clinical sign. Lung and liver biopsies were performed and fixed in neutral buffered 10% formalin and paraffin embedded. Blood samples were collected via cardiocentesis, and air sac abscesses were cultured. Bacterial strains were identified as Aeromonas sobria by 16S rDNA sequencing. Based on histological and bacterial examinations, the death of this snake was attributed to a septicemia, following an acute primary, or secondary exudative pneumonia. Aeromonas sp. is established as a potential pathogen in reptiles. Among this genus, Aeromonas hydrophila is the most frequently isolated. A. sobria has been reported as a primary pathogen in farmed perch (Perca fluviatilis) and humans. Conversely, few data are available concerning the pathogenicity of A. sobria in reptiles. Other non-bacterial agents (virus, fungus, endoparasites) or predisposing factors (such as obesity) can also be responsible for respiratory tract disease in snakes . Unfortunately, in the current case, virological investigations were not performed. [less ▲]

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See detailEpizootic rabbit enteropathy: Comparison of PCR-based RAPD fingerprints from virulent and non-virulent samples
Huybens, Nathalie ULg; Houeix, Julien ULg; Licois, Dominique et al

in Veterinary Journal (2011), 190(3), 416-417

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See detailEpizootic rabbit enteropathy inoculum (TEC4) antibiograms and antibiotic fractionation
Huybens, Nathalie ULg; Houeix, Julien ULg; Licois, Dominique et al

in Veterinary Research Communications (2011), 35

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See detailVaccination strategies against myxomavius infections: are we really doing the best?
Marlier, Didier ULg

in Tijdschrift voor Diergeneeskunde (2010), 134

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