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See detailCulicoides monitoring in Belgium in 2011: Analysis of spatio-temporal abundance, species diversity and Schmallenberg virus detection.
Losson, Bertrand ULiege; De Regge, N; Deblauwe, I et al

in Medical and Veterinary Entomology (2015), 29

In 2011, Culicoides (Diptera: Ceratopogonidae) were collected at 16 locations covering four regions of Belgium with Onderstepoort Veterinary Institute (OVI) traps and at two locations with Rothamsted ... [more ▼]

In 2011, Culicoides (Diptera: Ceratopogonidae) were collected at 16 locations covering four regions of Belgium with Onderstepoort Veterinary Institute (OVI) traps and at two locations with Rothamsted suction traps (RSTs). Quantification of the collections and morphological identification showed important variations in abundance and species diversity between individual collection sites, even for sites located in the same region. However, consistently higher numbers of Culicoides midges were collected at some sites compared with others. When species abundance and diversity were analysed at regional level, between-site variation disappeared. Overall, species belonging to the subgenus Avaritia together with Culicoides pulicaris (subgenus Culicoides) were the most abundant, accounting for 80% and 96% of all midges collected with RSTs and OVI traps, respectively. Culicoides were present during most of the year, with Culicoides obsoletus complex midges found from 9 February until 27 December. Real-time reverse-transcription polymerase chain reaction screening for Schmallenberg virus in the heads of collected midges resulted in the first detection of the virus in August 2011 and identified C. obsoletus complex, Culicoides chiopterus and Culicoides dewulfi midges as putative vector species. At Libramont in the south of Belgium, no positive pools were identified. [less ▲]

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See detailBluetongue Virus Detection By Real-Time Rt-Pcr In Culicoides Captured During The 2006 Epizootic In Belgium And Development Of An Internal Control
Vanbinst, T.; Vandenbussche, F.; Vandemeulebroucke, E. et al

in Transboundary and Emerging Diseases (2009), 56(5), 170-177

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See detailDistribution of potential bluetongue vectors on Belgium farms
Zimmer, Jean-Yves ULiege; Haubruge, Eric ULiege; Francis, Frédéric ULiege et al

in Veterinary Record : Journal of the British Veterinary Association (2008), 162(21), 700

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See detailVector monitoring at Belgian outbreak sites during the bluetongue epidemic of 2006.
De Deken, G.; Madder, M.; Deblauwe, I. et al

in Preventive Veterinary Medicine (2008), 87(1-2), 64-73

In response to the first bluetongue outbreak in Belgium a monitoring programme was started at the end of August 2006 to identify possible vectors transmitting the disease. Black light traps were deployed ... [more ▼]

In response to the first bluetongue outbreak in Belgium a monitoring programme was started at the end of August 2006 to identify possible vectors transmitting the disease. Black light traps were deployed at 36 outbreak sites and captured 1959 Culicoides specimens belonging to 16 different species. Eighty four percent of the biting midges captured belonged to the C. obsoletus complex, among them C. obsoletus s.s., C. dewulfi and C. scoticus, three suspected bluetongue vectors. The Veterinary and Agrochemical Research Centre detected viral RNA in pools of individuals belonging to this complex. Culicoides pulicaris, a potential bluetongue vector in Italy, should yet not be excluded as a possible vector in Belgium as this species was frequently found around outbreak sites, notwithstanding this species is not easily captured with the trapping techniques used during this survey. [less ▲]

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See detailBiting Midges Overwintering In Belgium
Losson, Bertrand ULiege; Mignon, Bernard ULiege; Paternostre, Julien ULiege et al

in Veterinary Record : Journal of the British Veterinary Association (2007), 160(13), 451-452

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See detailIn vitro titration of Theileria parva tick derived stabilates
Marcotty, T.; Speybroeck, N.; Berkvens, D. et al

in Parasitology (2004), 128(Part 2), 131-137

Immunization agairist the protozoan Theileria parva by infection and treatment has proved to be very efficient for the Control Of East Coast fever, an acute and often-fatal lymphoproliferative tick-borlic ... [more ▼]

Immunization agairist the protozoan Theileria parva by infection and treatment has proved to be very efficient for the Control Of East Coast fever, an acute and often-fatal lymphoproliferative tick-borlic disease of cattle in Eastern, Central and Southern Africa. The immunizing dose of live T. Parva sporozites used in this method is usually determined by in vitro titration. An alternative in vivo method of quantitification of sporozoites ill whole tick-derived stabilites is proposed. The method consists of incubating serially diluted T. Parva stabilities with boville peripheral blood lymphocytes, the host cell that is infected naturally. Allowing the cultures to incubate undisturbed for the full cultivation period (10 days) reduced the variability amoung replicate titrations. fungal contaminations were avoided by centrifuging stabilates at 400 g prior to the incubation, which did not precipiate sporozoites significantly. Fungistics, Nysatin and Flucytosine did not appear to interfere with the in vitro development of 2 stabilates but their effect on fungal growth was limited. In vitro titration data were compared to in vivo infection data for 2. In vivo titration of T. parva sporozoites should allow more ethicl and efficient research on the preparation and storage of T. Parva tick-derived stabilates. [less ▲]

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See detailLyophilisation and resuscitation of sporozoites of Theileria parva: preliminary experiments
Marcotty, T.; Berkvens, D.; Besa, R. K. et al

in Vaccine (2003), 22(2), 213-216

Lyophilisation of Theileria parva sporozoite stabilates used for immunisation of cattle against East Coast fever would greatly improve vaccine storage and delivery. We report three attempts to lyophilise ... [more ▼]

Lyophilisation of Theileria parva sporozoite stabilates used for immunisation of cattle against East Coast fever would greatly improve vaccine storage and delivery. We report three attempts to lyophilise and resuscitate the sporozoites of T parva. Sporozoites survived lyophilisation and were effective for immunisation. Lyophilised stabilate survived for 2 weeks at 5degreesC and for 12 weeks at -20degreesC. Although the viability of the stabilates was severely reduced during lyophilisation, this work suggests that this method has potential and should be considered for other Apicomplexan parasites such as Babesia sp. or Plasmodium sp. (C) 2003 Elsevier Ltd. All rights reserved. [less ▲]

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