References of "Madder, M"
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See detailBluetongue Virus Detection By Real-Time Rt-Pcr In Culicoides Captured During The 2006 Epizootic In Belgium And Development Of An Internal Control
Vanbinst, T.; Vandenbussche, F.; Vandemeulebroucke, E. et al

in Transboundary and Emerging Diseases (2009), 56(5), 170-177

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See detailDistribution Of Potential Bluetongue Vectors On Belgium Farms
Zimmer, Jean-Yves ULg; Haubruge, Eric ULg; Francis, Frédéric ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2008), 162(21), 700

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See detailVector monitoring at Belgian outbreak sites during the bluetongue epidemic of 2006.
De Deken, G.; Madder, M.; Deblauwe, I. et al

in Preventive Veterinary Medicine (2008), 87(1-2), 64-73

In response to the first bluetongue outbreak in Belgium a monitoring programme was started at the end of August 2006 to identify possible vectors transmitting the disease. Black light traps were deployed ... [more ▼]

In response to the first bluetongue outbreak in Belgium a monitoring programme was started at the end of August 2006 to identify possible vectors transmitting the disease. Black light traps were deployed at 36 outbreak sites and captured 1959 Culicoides specimens belonging to 16 different species. Eighty four percent of the biting midges captured belonged to the C. obsoletus complex, among them C. obsoletus s.s., C. dewulfi and C. scoticus, three suspected bluetongue vectors. The Veterinary and Agrochemical Research Centre detected viral RNA in pools of individuals belonging to this complex. Culicoides pulicaris, a potential bluetongue vector in Italy, should yet not be excluded as a possible vector in Belgium as this species was frequently found around outbreak sites, notwithstanding this species is not easily captured with the trapping techniques used during this survey. [less ▲]

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See detailBiting Midges Overwintering In Belgium
Losson, Bertrand ULg; Mignon, Bernard ULg; Paternostre, Julien ULg et al

in Veterinary Record : Journal of the British Veterinary Association (2007), 160(13), 451-452

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See detailIn vitro titration of Theileria parva tick derived stabilates
Marcotty, T.; Speybroeck, N.; Berkvens, D. et al

in Parasitology (2004), 128(Part 2), 131-137

Immunization agairist the protozoan Theileria parva by infection and treatment has proved to be very efficient for the Control Of East Coast fever, an acute and often-fatal lymphoproliferative tick-borlic ... [more ▼]

Immunization agairist the protozoan Theileria parva by infection and treatment has proved to be very efficient for the Control Of East Coast fever, an acute and often-fatal lymphoproliferative tick-borlic disease of cattle in Eastern, Central and Southern Africa. The immunizing dose of live T. Parva sporozites used in this method is usually determined by in vitro titration. An alternative in vivo method of quantitification of sporozoites ill whole tick-derived stabilites is proposed. The method consists of incubating serially diluted T. Parva stabilities with boville peripheral blood lymphocytes, the host cell that is infected naturally. Allowing the cultures to incubate undisturbed for the full cultivation period (10 days) reduced the variability amoung replicate titrations. fungal contaminations were avoided by centrifuging stabilates at 400 g prior to the incubation, which did not precipiate sporozoites significantly. Fungistics, Nysatin and Flucytosine did not appear to interfere with the in vitro development of 2 stabilates but their effect on fungal growth was limited. In vitro titration data were compared to in vivo infection data for 2. In vivo titration of T. parva sporozoites should allow more ethicl and efficient research on the preparation and storage of T. Parva tick-derived stabilates. [less ▲]

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See detailLyophilisation and resuscitation of sporozoites of Theileria parva: preliminary experiments
Marcotty, T.; Berkvens, D.; Besa, R. K. et al

in Vaccine (2003), 22(2), 213-216

Lyophilisation of Theileria parva sporozoite stabilates used for immunisation of cattle against East Coast fever would greatly improve vaccine storage and delivery. We report three attempts to lyophilise ... [more ▼]

Lyophilisation of Theileria parva sporozoite stabilates used for immunisation of cattle against East Coast fever would greatly improve vaccine storage and delivery. We report three attempts to lyophilise and resuscitate the sporozoites of T parva. Sporozoites survived lyophilisation and were effective for immunisation. Lyophilised stabilate survived for 2 weeks at 5degreesC and for 12 weeks at -20degreesC. Although the viability of the stabilates was severely reduced during lyophilisation, this work suggests that this method has potential and should be considered for other Apicomplexan parasites such as Babesia sp. or Plasmodium sp. (C) 2003 Elsevier Ltd. All rights reserved. [less ▲]

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