References of "Louette, Joel"
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See detailDevelopment of an absolute quantification method targeting growth hormone biomarkers using liquid chromatography coupled to isotope dilution mass spectrometry.
Kirsch, Stéphanie ULg; Widart, Joëlle ULg; Louette, Joel et al

in Journal of Chromatography. A (2007), 1153(1-2), 300-6

A method to perform absolute quantification of two biomarkers (IGF-1 and IGFBP-3) of growth hormone abuse has been developed. Isotope dilution is used with synthetically labelled peptides as internal ... [more ▼]

A method to perform absolute quantification of two biomarkers (IGF-1 and IGFBP-3) of growth hormone abuse has been developed. Isotope dilution is used with synthetically labelled peptides as internal standards. Peptide selection and multiple reaction monitoring design are discussed. A simple sample preparation based on the reduction and alkylation of cysteine residues followed by tryptic digestion provides a sufficient digestion of proteins. Serum samples fortified with increasing amounts of target proteins are analysed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) on a triple quadrupole mass spectrometer. Specificity is ensured by the selection of sequences with no homology in BLAST, as well as retention time deviation check, and ion ratio monitoring. Linearity is studied in terms of calibration curves. These curves for IGFBP-3 and IGF-1 are generated with mean slopes of 0.055 and 0.065, intercepts of 0.107 and -0.011, and with coefficients of correlation of 0.95 and 0.98, respectively. These curves result from the addition of proteins to the serum. Risks of variations related to potential matrix effects are therefore reduced, as well as probable variations related to the digestion steps. The working concentration ranges are 4-10 ng/microl for IGFBP-3 and 2-8 ng/microl for IGF-1. Preliminary data regarding repeatability show that relative standard deviations (RSDs) range between 13 and 32% for IGFBP-3 and between 7 and 29% for IGF-1. [less ▲]

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See detailPROCEDE ET DISPOSITIF DE PERMEABILISATION DE CELLULES VIVANTES
Servais, Christian ULg; Louette, Joël

Patent (1991)

A method and a device for making cell membranes permeable using electrical discharges (electroporation), wherein at least one short electrical discharge is applied to a suspension containing cells to be ... [more ▼]

A method and a device for making cell membranes permeable using electrical discharges (electroporation), wherein at least one short electrical discharge is applied to a suspension containing cells to be made permeable and molecules to be accommodated therein, in order to generate in said suspension a high-value electric field, whereafter at least one longer electrical discharge is applied thereto to generate a lower-value electric field therein. [less ▲]

Detailed reference viewed: 10 (2 ULg)