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See detailUse of complementary biophysical approaches to study the interactions of fatty acid hydroperoxides with biomimetic plant plasma membranes
Deboever, Estelle ULg; Nasir, Mehmet Nail ULg; Deleu, Magali ULg et al

Poster (2017, January 20)

In the actual context, biopesticides have emerged as a main alternative to conventional agriculture1. Hence, elicitors are metabolites naturally produced by microorganisms, pathogenic or not, and plants ... [more ▼]

In the actual context, biopesticides have emerged as a main alternative to conventional agriculture1. Hence, elicitors are metabolites naturally produced by microorganisms, pathogenic or not, and plants which are able to induce the natural resistance of plants. Also, they have proved to be excellent candidates for biological control. In this context, the lipoxygenase pathway leads to the formation of fatty acid degradation products, called oxylipins, which appear to be crucial agents in plant defence mechanisms2,3. Moreover, with their broad spectrum of action and their possible inducibility, oxylipins appear to be promising candidates for their use as elicitors4. This work focuses on two hydroperoxy-derived oxylipins, the 13(S)-hydroperoxy-octadecadienoic acid (13-HPOD) and the 13(S)-hydroperoxy-octadecatrienoic acid (13-HPOT). The study of the interaction of such compounds with representative plant plasma membrane lipids is essential to understand plant resistance mechanisms. Several in silico and experimental techniques of biophysics showed that acyl-hydroperoxides have significant adsorption capacity and a strong affinity for model membranes. They may also penetrate biological membrane but no permeabilisation effect was observed in this work. Slight conformational differences seem to have a significant impact on their ability to interact with plant plasma membranes. Based on these results, further investigation of the interactions of fatty acids hydroperoxides, even more on the 9-forms, with plant plasma membranes and eventually in the presence of phytopathogenic species, would allow a better understanding of the innate immunity and, on the longer term, could lead to the development of new elicitors with biological mechanisms potentially independent of membrane protein receptors. [less ▲]

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See detailMolecular dynamics study of micelle proeprties according to their size
Lebecque, Simon ULg; Crowet, Jean-Marc ULg; Nasir, Mehmet Nail ULg et al

in Journal of Molecular Graphics and Modelling (2017)

Surfactants are molecules able to spontaneously self-assemble to form aggregates with well-defined properties, such as spherical micelles, planar bilayers, cylindrical micelles or vesicles. Micelles have ... [more ▼]

Surfactants are molecules able to spontaneously self-assemble to form aggregates with well-defined properties, such as spherical micelles, planar bilayers, cylindrical micelles or vesicles. Micelles have notably several applications in many domains, such as drug delivery or membrane protein solubilization. In this context, the study of micelle formation in relation with the structural and physico-chemical properties of surfactants is of great interest to better control their use in the different application fields. In this work, we use the MD approach developed by Yoshii et al. and extend it to surfactants with different structures. We aim to systematically investigate different micellar properties as a function of the aggregates size by a molecular dynamics approach, to get an insight into the micellar organization and to collect some relevant descriptors about micelle formation. For this, we perform short MD simulations of preformed micelles of various sizes and analyze three parameters for each micelle size, namely the eccentricity of the micelles, the hydrophobic/hydrophilic surface ratio and the hydrophobic tails hydration. If these parameters are known descriptors of micelles, they were not yet studied in this way by MD. We show that eccentricity, used as “validator” parameter, exhibits minimal values when the aggregate size is close to the experimental aggregation number for surfactants that are known to form spherical micelles. This hence indicates that our methodology gives consistent results. The evolution of the two descriptors follows another scheme, with a sharp increase and decrease, respectively, followed by a leveling-off. The aggregate sizes at which this stabilization starts to occur are close to the respective aggregation number of each surfactant. In our approach, we validate the use of these descriptors to follow micelle formation by MD, from “simple” surfactants to more complex structures, like lipopeptides. Our calculations also suggest that some peculiar behavior, like that of TPC, can be highlighted by our approach. In the context of peptidic surfactants, our methodology could further help to improve computer simulations combined to molecular thermodynamic models to predict micellar properties of those more complex amphiphilic molecules. [less ▲]

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See detailMembrane Interactions of Natural Cyclic Lipodepsipeptides of the Viscosin Group
Geudens, Niels; Nasir, Mehmet Nail ULg; Crowet, Jean-Marc ULg et al

in Biochimica et Biophysica Acta - Biomembranes (2017), 1859(3), 331-339

Many Pseudomonas spp. produce cyclic lipodepsipeptides (CLPs), which, besides their role in biological functions such as motility, biofilm formation and interspecies interactions, are antimicrobial. It ... [more ▼]

Many Pseudomonas spp. produce cyclic lipodepsipeptides (CLPs), which, besides their role in biological functions such as motility, biofilm formation and interspecies interactions, are antimicrobial. It has been established that interaction with the cellular membrane is central to the mode of action of CLPs. In this work, we focus on the CLPs of the so-called viscosin group, aiming to assess the impact of the main structural variations observed within this group on both the antimicrobial activity and the interaction with model membranes. The antimicrobial activity of viscosin, viscosinamide A, WLIP and pseudodesmin A were all tested on a broad panel of mainly Gram-positive bacteria. Their capacity to permeabilize or fuse PG/PE/cardiolipin model membrane vesicles is assessed using fluorescent probes. We find that the Glu2/Gln2 structural variation within the viscosin group is the main factor that influences both the membrane permeabilization properties and the minimum inhibitory concentration of bacterial growth, while the configuration of the Leu5 residue has no apparent effect. The CLPmembrane interactions were further evaluated using CD and FT-IR spectroscopy on model membranes consisting of PG/PE/cardiolipin or POPC with or without cholesterol. In contrast to previous studies, we observe no conformational change upon membrane insertion. The CLPs interact both with the polar heads and aliphatic tails of model membrane systems, altering bilayer fluidity, while cholesterol reduces CLP insertion depth [less ▲]

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See detailGROMPALA: a membrane-implicit modelling method to screen lipid-interacting molecules
Steinhauer, Sven; Crowet, Jean-Marc ULg; Brasseur, Robert ULg et al

in Mukhametov, Azat (Ed.) Software and techniques for Bio-molecular modeling (2016)

Detailed reference viewed: 74 (5 ULg)
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See detailAnalysis of the interactions of fatty acyds hydroperoxides with biomimetic membranes by complementary biophysical approaches
Deboever, Estelle ULg; Nasir, Mehmet Nail ULg; Deleu, Magali ULg et al

Poster (2016, November 16)

In the actual context, biopesticides have emerged as a main alternative to conventional agriculture1. Hence, elicitors are metabolites naturally produced by microorganisms, pathogenic or not, and plants ... [more ▼]

In the actual context, biopesticides have emerged as a main alternative to conventional agriculture1. Hence, elicitors are metabolites naturally produced by microorganisms, pathogenic or not, and plants which are able to induce the natural resistance of plants. Also, they have proved to be excellent candidates for biological control. In this context, the lipoxygenase pathway leads to the formation of fatty acid degradation products, called oxylipins, which appear to be crucial agents in plant defence mechanisms2,3. Moreover, with their broad spectrum of action and their possible inducibility, oxylipins appear to be promising candidates for their use as elicitors4. This work focuses on two hydroperoxy-derived oxylipins, the 13(S)-hydroperoxy-octadecadienoic acid (13-HPOD) and the 13(S)-hydroperoxy-octadecatrienoic acid (13-HPOT). The study of the interaction of such compounds with representative plant plasma membrane lipids is essential to understand plant resistance mechanisms. Several in silico and experimental techniques of biophysics showed that acyl-hydroperoxides have significant adsorption capacity and a strong affinity for model membranes. They may also penetrate biological membrane but no permeabilisation effect was observed in this work. Slight conformational differences seem to have a significant impact on their ability to interact with plant plasma membranes. Based on these results, further investigation of the interactions of fatty acids hydroperoxides, even more on the 9-forms, with plant plasma membranes and eventually in the presence of phytopathogenic species, would allow a better understanding of the innate immunity and, on the longer term, could lead to the development of new elicitors with biological mechanisms potentially independent of membrane protein receptors. [less ▲]

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See detailDevelopment of a new method for arabidopsides extraction and purification
Genva, Manon ULg; Andersson, Mats X; Nasir, Mehmet Nail ULg et al

Poster (2016, November 16)

In the present work, a new method for arabidopsides extraction and purification was developed.

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See detailPlant esterified oxylipins: structure – function relationship
Genva, Manon ULg; Andersson, Mats X.; Nasir, Mehmet Nail ULg et al

Poster (2016, September)

In the present work, high quantities of arabidopsides were extracted and purified from Arabidopsis thaliana L.

Detailed reference viewed: 41 (12 ULg)
See detailCaractérisation des mécanismes impliqués dans la perception de rhamnolipides naturels et synthétiques chez Arabidopsis thaliana
Luzuriaga Loaiza, Walter ULg; Schellenberger, Romain; Obounou Akong, Firmin et al

Scientific conference (2016, July 05)

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See detailCover Letter of The journal of Biological Chemistry
Sautrey, Guillaume; El Khoury, M; Dos Giros, A et al

in The journal of Biological Chemistry (2016), 291(26),

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See detailModeling of the cyclic lipodepsipeptide Pseudodesmin A self-assembly through molecular dynamic simulations
Crowet, Jean-Marc ULg; Sinnaeve, Davy; Fehér, Kristina et al

Poster (2016, April)

Detailed reference viewed: 14 (0 ULg)