References of "Linden, Annick"
     in
Bookmark and Share    
Full Text
Peer Reviewed
See detailUsutu virus, Belgium, 2016
Garigliany, Mutien-Marie ULg; Linden, Annick ULg; Gilliaux, Gautier ULg et al

in Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases (2017), 48(1), 116-119

During late summer 2016, in a northwest European region extending over Belgium, the Netherlands and the eastern border of the German state of North Rhine Westphalia, an outbreak of wild bird deaths ... [more ▼]

During late summer 2016, in a northwest European region extending over Belgium, the Netherlands and the eastern border of the German state of North Rhine Westphalia, an outbreak of wild bird deaths occurred similar to those reported on the continent since 1996. Dead birds were necropsied and examined by complementary methods. Pathologic and immunohistological investigations strongly suggested an infection by Usutu virus. Subsequently, genomic segments of the said virus were detected, the virus was isolated and its complete genome was sequenced. The strain, designated Usutu-LIEGE, is a close phylogenetic relative of those isolated in Germany which form a distinct group within the USUV phylogeny, the so-called Europe_3 lineage. Should this outbreak recapitulate the characteristics of those in southwest Germany in 2011 and in/around Vienna (Austria) in 2001, it is expected that specific avian populations in the affected area will face a significant reduction in size for a few years. [less ▲]

Detailed reference viewed: 32 (9 ULg)
Full Text
Peer Reviewed
See detailWidespread activity of multiple lineages of Usutu virus, western Europe, 2016.
Cadar, Daniel; Luhken, Renke; van der Jeugd, Henk et al

in Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin (2017), 22(4),

In the summer of 2016, Belgium, France, Germany and the Netherlands reported widespread Usutu virus (USUV) activity based on live and dead bird surveillance. The causative USUV strains represented four ... [more ▼]

In the summer of 2016, Belgium, France, Germany and the Netherlands reported widespread Usutu virus (USUV) activity based on live and dead bird surveillance. The causative USUV strains represented four lineages, of which two putative novel lineages were most likely recently introduced into Germany and spread to other western European countries. The spatial extent of the outbreak area corresponded with R0 values > 1. The occurrence of the outbreak, the largest USUV epizootic registered so far in Europe, allowed us to gain insight in how a recently introduced arbovirus with potential public health implications can spread and become a resident pathogen in a naive environment. Understanding the ecological and epidemiological factors that drive the emergence or re-emergence of USUV is critical to develop and implement timely surveillance strategies for adequate preventive and control measures. Public health authorities, blood transfusion services and clinicians in countries where USUV was detected should be aware of the risk of possible USUV infection in humans, including in patients with unexplained encephalitis or other neurological impairments, especially during late summer when mosquito densities peak. [less ▲]

Detailed reference viewed: 15 (1 ULg)
Full Text
See detailOccurence of the new variant of rabbit haemorrhagic disease virus (RHDV2) in wild populations of rabbits in Southern Belgium
Volpe, Rosario ULg; Lesenfants, Christophe ULg; Paternostre, Julien ULg et al

Poster (2016, October 07)

Rabbit haemorrhagic disease (RHD) is a highly infectious and fatal disease of the European rabbit (Oryctolagus cuniculus), responsible for important economic losses in the rabbit industry. The ... [more ▼]

Rabbit haemorrhagic disease (RHD) is a highly infectious and fatal disease of the European rabbit (Oryctolagus cuniculus), responsible for important economic losses in the rabbit industry. The aetiological agent of the disease is a RNA virus (RHDV, Lagovirus, Caliciviridae) first detected in China in 1984. Currently RHDV is endemic in most parts of Europe, Asia and North Africa. Phylogenetic analyses of RHDV strains have identified 3 distinct groups : the classic RHDV, the variant RHDVa and RHDV2. This latter has been detected in France for the first time in 2010 in domestic and wild rabbits (Le Gall-Reculé G et al., 2013) and since then has spread throughout Europe, replacing the circulating RHDV/RHDVa strains in most european countries. RHDV2 has already been detected in Belgium in rabbitries (Marlier D et al., 2014). Here, we report for the first time the presence of RHDV2 in wild rabbits in Southern Belgium. In november 2015, the Surveillance Network of Wildlife Diseases received seven dead wild rabbits for necropsy. The discovery of 7 fresh carcasses found at the same time in a same area (Hainaut province) emphasised the infectious or intoxication hypothesis as cause of death. Postmortem examinations were performed at the Faculty of Veterinary Medicine (FVM) of the University of Liege according to a systematic protocol based on gross lesions, histopathological and targeted microbiological analysis. For necropsy, each rabbit (1) was weighted and age was determined by the presence/absence of the distal ulna protuberance, (2) stomach was investigated to exclude poison, (3) spleen was systematically driven into Yersinia CIN culture media for detection of Yersinia pseudotuberculosis, (4) lungs and livers were systematically (a) packaged into 10% formaldehyde solution for histopathology analysis (Service of Pathology, FVM) and (b) frozen at -20°C for RHDV analysis (Scanelis Laboratory,Toulouse, France) and finally (6) feces were gathered for parasitology (Service of Parasitology, FVM). At necropsy, animals (5 adults: 3 males/2 females and 2 juveniles: 1 male/1 female) were in good condition. No hematomas or broken bones were detected, only one displayed clues of diarrhoea. Examinations of the carcasses showed congestion of lungs/kidneys and livers were macroscopically normal. No foreign body or suspicious particles was seen in the stomachs, only one rabbit was hardly infested by tapeworms in the gut. Histopathological examination revealed haemorragic lung lesions in one animal while 5 of them presented severe necrotic hepatitis, sometimes associated with peri-angiocholitis. Only one animal presented an abnormal high rate of coccidia in feces. Samples of livers were sent to Scanelis Laboratory for RHDV RT-qPCR diagnostic. The results were positive for the new variant RHDV2 in 5 out of the 7 rabbit livers. All the samples were negative for the classic RHDV. To determine if RHDV2 was already present before 2015 in wild rabbits in the region, we tested a series of livers that had been collected in 2013 and 2014 for a retrospective study. Among the 25 rabbit livers checked, 12 presented necrotic hepatitis and were sent for analysis. Ten were confirmed positive by RT-qPCR for RHDV2. [less ▲]

Detailed reference viewed: 46 (5 ULg)
Full Text
See detailOccurence of the new variant of rabbit haemorrhagic disease virus (RHDV2) in wild populations of rabbits in Southern Belgium
Volpe, Rosario ULg; Lesenfants, Christophe ULg; Paternostre, Julien ULg et al

Conference (2016, September 09)

Rabbit haemorrhagic disease (RHD) is a highly infectious and fatal disease of the European rabbit (Oryctolagus cuniculus), responsible for important economic losses in the rabbit industry. The ... [more ▼]

Rabbit haemorrhagic disease (RHD) is a highly infectious and fatal disease of the European rabbit (Oryctolagus cuniculus), responsible for important economic losses in the rabbit industry. The aetiological agent of the disease is a RNA virus (RHDV, Lagovirus, Caliciviridae) first detected in China in 1984. Currently RHDV is endemic in most parts of Europe, Asia and North Africa. Phylogenetic analyses of RHDV strains have identified 3 distinct groups : the classic RHDV, the variant RHDVa and RHDV2. This latter has been detected in France for the first time in 2010 in domestic and wild rabbits (Le Gall-Reculé G et al., 2013) and since then has spread throughout Europe, replacing the circulating RHDV/RHDVa strains in most european countries. RHDV2 has already been detected in Belgium in rabbitries (Marlier D et al., 2014). Here, we report for the first time the presence of RHDV2 in wild rabbits in Southern Belgium. In november 2015, the Surveillance Network of Wildlife Diseases received seven dead wild rabbits for necropsy. The discovery of 7 fresh carcasses found at the same time in a same area (Hainaut province) emphasised the infectious or intoxication hypothesis as cause of death. Postmortem examinations were performed at the Faculty of Veterinary Medicine (FVM) of the University of Liege according to a systematic protocol based on gross lesions, histopathological and targeted microbiological analysis. For necropsy, each rabbit (1) was weighted and age was determined by the presence/absence of the distal ulna protuberance, (2) stomach was investigated to exclude poison, (3) spleen was systematically driven into Yersinia CIN culture media for detection of Yersinia pseudotuberculosis, (4) lungs and livers were systematically (a) packaged into 10% formaldehyde solution for histopathology analysis (Service of Pathology, FVM) and (b) frozen at -20°C for RHDV analysis (Scanelis Laboratory,Toulouse, France) and finally (6) feces were gathered for parasitology (Service of Parasitology, FVM). At necropsy, animals (5 adults: 3 males/2 females and 2 juveniles: 1 male/1 female) were in good condition. No hematomas or broken bones were detected, only one displayed clues of diarrhoea. Examinations of the carcasses showed congestion of lungs/kidneys and livers were macroscopically normal. No foreign body or suspicious particles was seen in the stomachs, only one rabbit was hardly infested by tapeworms in the gut. Histopathological examination revealed haemorragic lung lesions in one animal while 5 of them presented severe necrotic hepatitis, sometimes associated with peri-angiocholitis. Only one animal presented an abnormal high rate of coccidia in feces. Samples of livers were sent to Scanelis Laboratory for RHDV RT-qPCR diagnostic. The results were positive for the new variant RHDV2 in 5 out of the 7 rabbit livers. All the samples were negative for the classic RHDV. To determine if RHDV2 was already present before 2015 in wild rabbits in the region, we tested a series of livers that had been collected in 2013 and 2014 for a retrospective study. Among the 25 rabbit livers checked, 12 presented necrotic hepatitis and were sent for analysis. Ten were confirmed positive by RT-qPCR for RHDV2. [less ▲]

Detailed reference viewed: 48 (2 ULg)
Full Text
See detailSarcoptic mange infection in red foxes (Vulpes vulpes) in Belgium
Volpe, Rosario ULg; Caron, Yannick ULg; Lesenfants, Christophe ULg et al

Poster (2016, August)

Eight cases of sarcoptic mange in foxes were reported during summer 2014 (n=3) and winter 2015 (n= 5) in Belgium. All animals came from the same restricted forest zones near urbanized areas and some of ... [more ▼]

Eight cases of sarcoptic mange in foxes were reported during summer 2014 (n=3) and winter 2015 (n= 5) in Belgium. All animals came from the same restricted forest zones near urbanized areas and some of them were discovered in private gardens (Forest District of Thuin, Hainaut, Belgium). Three of them were found dead, the others were shot for sanitary reasons. At necropsy, all of them presented large areas of alopecia and extensive skin lesions such as hyperkeratosis and suppurative lacerations. Animals presented poor body condition and some of them were severely emaciated (absence of visceral fat). Mange has profound influences on population since, if untreated, death follows in four to six months. In the present cases, cutaneous scrapings and histopathological examinations were systematically performed. Numerous parasites were observed in skin scrapings and marked dermo-epidermatitis with several parasites was observed in histopathological sections. These results suggest an outbreak of sarcoptic mange in red foxes in a limited area of the country. This is a first record for the country. Furthermore, investigations on lungs and digestive tracts performed on 3 of these foxes revealed they were also infected by parasites transmissible to pets and/or humans: Angiostrongylus vasorum was observed in the respiratory tract of one fox whereas Toxocara canis, Uncinaria stenocephala, Taenia spp. and Echinococcus multilocularis worms were detected in the digestive tract. In conclusion, urban foxes represent a source of parasites of public health and veterinary importance and foxes surveillance should be strengthened in these areas. [less ▲]

Detailed reference viewed: 48 (2 ULg)
Full Text
Peer Reviewed
See detailComplete coding sequence of one H9 and three H7 low pathogenic influenza viruses circulating in wild birds in Belgium, 2009-2012
Van Borm, Steven; Rosseel, Toon; Marché, Sylvie et al

in Genome Announcements (2016), 4(3),

Detailed reference viewed: 13 (3 ULg)
Full Text
Peer Reviewed
See detailOne decade of active avian influenza wild bird surveillance in Belgium showed a higher viroprevalence in hunter-harvested than live-ringed birds
Steensels, Mieke; Vangeluwe, Didier; Linden, Annick ULg et al

in Avian Diseases (2016), 60(1s), 387-393

Detailed reference viewed: 23 (4 ULg)
Full Text
Peer Reviewed
See detailMeningitis and orchitis in a hare (Lepus Europaeus) infected with Francisella tularensis
Grégoire, Fabien; Cassart, Dominique ULg; Desmecht, Daniel ULg et al

in Veterinary Records Case Reports (2016), 4(e000306),

Detailed reference viewed: 21 (4 ULg)
Full Text
Peer Reviewed
See detailBelgian Wildlife as Potential Zoonotic Reservoir of Hepatitis E virus
Thiry, Damien ULg; Mauroy, Axel ULg; Saegerman, Claude ULg et al

in Transboundary and Emerging Diseases (2015)

Detailed reference viewed: 56 (18 ULg)
See detailRéseau de surveillance sanitaire de la faune sauvage en Belgique
Linden, Annick ULg

Scientific conference (2015, March)

Detailed reference viewed: 8 (5 ULg)
Full Text
See detailSinuso-nasal adenocarcinoma in a roe deer (Capreolus capreolus)
Volpe, Rosario ULg; Cassart, Dominique ULg; Neukermans, Axel et al

Poster (2014, October)

Detailed reference viewed: 45 (10 ULg)
Full Text
Peer Reviewed
See detailMolecular evidence of Anaplasma phagocytophilum in wild boar (Sus scrofa) in Belgium
Nahayo, Adrien; Bardiau, Marjorie ULg; Volpe, Rosario ULg et al

in BMC Veterinary Research (2014), 10(80),

Anaplasma phagocytophilum is a tick-borne pathogen of veterinary and human importance. Both ticks as vectors and vertebrates as reservoir hosts are essential for the cycle maintenance of this bacterium ... [more ▼]

Anaplasma phagocytophilum is a tick-borne pathogen of veterinary and human importance. Both ticks as vectors and vertebrates as reservoir hosts are essential for the cycle maintenance of this bacterium. Currently, the whole range of animal species reservoirs for A. phagocytophilum in natural environment is still unknown. Therefore, the aim of this study was to estimate the prevalence of infection with A. phagocytophilum in the wild boar population in southern Belgium. [less ▲]

Detailed reference viewed: 56 (12 ULg)
See detailHepatitis E virus infection in suids and cervids in southern Belgium
Thiry, Damien ULg; Mauroy, Axel ULg; Fett, Thomas ULg et al

Conference (2014, April)

Detailed reference viewed: 33 (5 ULg)
See detailProblématique de la tuberculose bovine en faune sauvage
Linden, Annick ULg

Conference given outside the academic context (2014)

Detailed reference viewed: 10 (3 ULg)