  Olfactory Receptors; Schurmans, Stéphane  ; et alin Peroutka, S. (Ed.) Handbook of Receptors and Channels: G Protein Coupled Receptors (1994) Detailed reference viewed: 8 (1 ULg)Génétique moléculaire des récepteurs olfactifs; ; Schurmans, Stéphane et alin Medecine Sciences : M/S (1994), 10 Les gènes codant pour les récepteurs olfactifs ont été isolés à partir d'une banque d'ADNc de la muqueuse olfactive. Ils constituent une famille très étendue (plusieurs centaines de gènes) codant pour des ... [more ▼] Les gènes codant pour les récepteurs olfactifs ont été isolés à partir d'une banque d'ADNc de la muqueuse olfactive. Ils constituent une famille très étendue (plusieurs centaines de gènes) codant pour des récepteurs à sept passages transmembranaires couplés, par l'intermédiaire de protéines G, à l'activation de l'adénylyl cyclase et de la phospholipase C. La grande diversité des récepteurs olfactifs réside surtout dans les domaines transmembranaires III, IV, V et VI, qui portent les sites de liaison des ligands, les molécules odorantes, et répond sans doute à la diversité des ligands [less ▲] Detailed reference viewed: 25 (1 ULg)Cloning and characterization of G protein-coupled receptors; ; et al in Advances in Second Messenger and Phosphoprotein Research (1993), 28 Detailed reference viewed: 9 (2 ULg)Construction of a bovine genomic library of large yeast artificial chromosome clones; ; et al in Genomics (1993), 18(2), 270-276 We have constructed a yeast artificial chromosome (YAC) library derived from bull fibroblasts in primary culture. The library consists of 21,500 clones arranged on 224 gridded plates (96 or 8×12 positions ... [more ▼] We have constructed a yeast artificial chromosome (YAC) library derived from bull fibroblasts in primary culture. The library consists of 21,500 clones arranged on 224 gridded plates (96 or 8×12 positions) and in 2×224 96-well microplates maintained as permanent frozen stocks. An average insert size of 750 kb was estimated from the analysis of 200 randomly selected YACs, giving a sixfold coverage of the bovine genome for the total library. A screening strategy based on the polymerase chain reaction that allows the identification of individual clones has been devised. Screening of the library with six locus-specific markers of interest led to the isolation of 34 positive clones [less ▲] Detailed reference viewed: 6 (2 ULg)Microsatellite mapping of the gene causing weaver disease in cattle will allow the study of an associated QTLGeorges, Michel ; ; et alin Proceedings of the National Academy of Sciences of the United States of America (1993), 90 A genetic disease in cattle, progressive degenerative myeloencephalopathy (weaver disease), is associated with increased milk production. This association could result from population stratification, from ... [more ▼] A genetic disease in cattle, progressive degenerative myeloencephalopathy (weaver disease), is associated with increased milk production. This association could result from population stratification, from a pleiotropic effect of a single gene, or from linkage disequilibrium between the gene causing weaver disease and a quantitative trait locus (QTL) for milk production. To test these hypotheses, we performed an extensive linkage study in a bovine pedigree segregating for the weaver condition and identified a microsatellite locus (TGLA116) closely linked to the weaver gene (zmax, 8.15; theta, 0.03). TGLA116 and, by extension, the weaver locus were assigned to bovine synteny group 13. This microsatellite can be used to identify weaver carriers, to select against this genetic defect, and to study the effect of the corresponding chromosomal region on milk production in Brown Swiss and other breeds of cattle. [less ▲] Detailed reference viewed: 12 (1 ULg)Microsatellite mapping of a gene affecting horn development in Bos taurusGeorges, Michel ; ; et alin Nature Genetics (1993), 4(2), 206-210 The presence or absence of horns in Bos taurus is thought to be under the genetic control of the autosomal polled locus, characterized by two alleles: P dominant over p, and causing the polled or hornless ... [more ▼] The presence or absence of horns in Bos taurus is thought to be under the genetic control of the autosomal polled locus, characterized by two alleles: P dominant over p, and causing the polled or hornless phenotype. We have demonstrated genetic linkage between the polled locus and two microsatellite markers, GMPOLL-1 and GMPOLL-2, and have assigned the corresponding linkage group to bovine chromosome 1. This confirms the existence of the postulated polled locus and the hypothesized inheritance pattern. It will allow marker assisted selection for the polledness trait in breeding programs and is a first step towards positional cloning and molecular study of a gene that has been subjected to both natural and artificial selection. [less ▲] Detailed reference viewed: 18 (3 ULg)Expression of members of the putative olfactory receptor gene family in mammalian germ cells.; ; Schurmans, Stéphane et alin Nature (1992), 355 A series of genomic and complementary DNA clones encoding new putative members of G protein-coupled receptors were isolated using homology cloning and low-stringency polymerase chain reaction. Among the ... [more ▼] A series of genomic and complementary DNA clones encoding new putative members of G protein-coupled receptors were isolated using homology cloning and low-stringency polymerase chain reaction. Among the unidentified receptors ('orphan receptors'), a human genomic clone (HGMP07) was characterized by the presence of its transcripts in the testis and by its belonging to a large subfamily of genes sharing extensive sequence similarities. Sequence comparison demonstrated that this gene subfamily is the human counterpart of the putative rat olfactory receptors cloned recently. Another 48 members of the family were cloned. Northern blotting further demonstrated the presence of olfactory receptor transcripts in germ cells. Our finding suggests that a common receptor gene family encodes olfactory receptors and sperm cell receptors that could be involved in chemotaxis during fertilization [less ▲] Detailed reference viewed: 21 (5 ULg) |
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