References of "Letellier, C"
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See detailEpidemiology of pestivirus infection in wild ungulates of the French South Alps
Martin, C.; Letellier, C.; Caij, B. et al

in Veterinary Microbiology (2011), 147(3-4), 320-328

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See detailConcepts in the prevention of bovine respiratory disease.
Makoschey, B.; Lekeux, Pierre ULg; Lacroux, C. et al

in Berliner und Munchener Tierarztliche Wochenschrift (2008), 121(11-12), 446-449

The bovine respiratory disease (BRD) complex requires further research both, to fully understand the disease from the different perspectives as well as to develop new tools and strategies for vaccination ... [more ▼]

The bovine respiratory disease (BRD) complex requires further research both, to fully understand the disease from the different perspectives as well as to develop new tools and strategies for vaccination and treatment was the conclusion at a recent BRD symposium in Rome, Italy. A group of scientist across Europe followed the invitation of Prof. E. Thiry (University of Liège, Belgium) to convene for a 2 days workshop type symposium sponsored by Intervet/Schering-Plough Animal Health (Fig. 1 – group picture) [less ▲]

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See detailEffects of exercise and oral antioxidant supplementation enriched in (n-3) fatty acids on blood oxidant markers and erythrocyte membrane fluidity in horses
De Moffarts, Brieuc; Portier, Karine; Kirschvink, Nathalie et al

in Veterinary Journal (2007), 174(1), 113-121

The aim of this study was to investigate in a placebo-controlled field study the effect of a (n - 3)-vitamin supplementation on erythrocyte membrane fluidity (ENIF), oxidant/antioxidant markers and ... [more ▼]

The aim of this study was to investigate in a placebo-controlled field study the effect of a (n - 3)-vitamin supplementation on erythrocyte membrane fluidity (ENIF), oxidant/antioxidant markers and plasmatic omega 3/omega 6 fatty acid ratio (FAR) in 12 eventing horses. Venous blood was sampled at rest before (PRE) and after (POST) a three week treatment period with either the supplement (group S, n = 6) or a placebo (group P, n = 6) as well as after 15 min (POST E15') and 24 h (POST E24h) after a standardised exercise test. The following markers were analysed: EMF, plasma antioxidant capacity of water and lipid soluble components, ascorbic acid, uric acid (UA), glutathione (reduced: GSH, oxidised: GSSG), vitamin E (Vit E), beta-carotene, glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity, selenium, copper (Cu), zinc (Zn), oxidised proteins (Protox), lipid peroxides (Pool) and FAR. EMF did not differ between group S and P after treatment, but GPx remained unchanged in group S whereas it decreased in group P and plasma Cu/Zn ratio remained unchanged whereas it increased in group P. FAR were significantly increased in group S. Exercise induced a significant decrease of EMF (POST vs. E24h) in both groups, but which was significantly lower at E15' in group S than in group P. Exercise induced a significant increase of UA and ACW (POST vs. E15') and Protox (POST vs. E24h) in both groups. An exercise-related decrease in GSH and Pool (POST vs. E15') was found in group P, whereas Vit E and FAR (POST vs. E24h) significantly decreased in both groups. The study showed that exercise induced a decrease in ENIF in horses associated with changes of blood oxidative balance. The (omega-3)vitamin supplementation tested improved the oxidative balance poorly but delayed the exercise-induced decrease of EMF and increased the FAR. [less ▲]

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See detailSequence-optimised E2 constructs from BVDV-1b and BVDV-2 for DNA immunisation in cattle
Couvreur, B.; Letellier, C.; Olivier, Fabrice ULg et al

in Veterinary Research (2007), 38(6, NOV-DEC), 819-834

We report DNA immunisation experiments in cattle using plasmid constructs that encoded glycoprotein E2 from bovine viral diarrhoea virus (BVDV)-1 (E2.1) and BVDV-2 (E2.2). The coding sequences were ... [more ▼]

We report DNA immunisation experiments in cattle using plasmid constructs that encoded glycoprotein E2 from bovine viral diarrhoea virus (BVDV)-1 (E2.1) and BVDV-2 (E2.2). The coding sequences were optimised for efficient expression in mammalian cells. A modified leader peptide sequence from protein gD of BoHV1 was inserted upstream of the E2 coding sequences for efficient membrane export of the proteins. Recombinant E2 were efficiently expressed in COS7 cells and they presented the native viral epitopes as judged by differential recognition by antisera from cattle infected with BVDV-1 or BVDV-2. Inoculation of pooled plasmid DNA in young cattle elicited antibodies capable of neutralising viral strains representing the major circulating BVDV genotypes. [less ▲]

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See detailReal Time RT-PCR for the detection and quantitation of bovine respiratory syncytial virus.
Boxus, Mathieu ULg; Letellier, C.; Kerkhofs, P.

in Journal of Virological Methods (2005), 125(2), 125-30

A quantitative Real Time RT-PCR assay was developed to detect and quantify bovine respiratory syncytial virus (BRSV) in the respiratory tract of infected animals. A pair of primers and a TaqMan probe ... [more ▼]

A quantitative Real Time RT-PCR assay was developed to detect and quantify bovine respiratory syncytial virus (BRSV) in the respiratory tract of infected animals. A pair of primers and a TaqMan probe targeting conserved regions of the nucleoprotein gene of BRSV were designed. The detection limit of the assay was shown to be 10(3) RNA copies and standard curve demonstrated a linear range from 10(3) to 10(8) copies as well as an excellent reproducibility. The efficiency of the BRSV Real Time RT-PCR was then assessed by detecting BRSV in lungs, tracheas and bronchoalveaolar fluids (BAL) samples of experimentally infected calves. The assay was shown to be 100 times more sensitive than conventional RT-PCR and was more efficient for BRSV diagnosis. Finally, the Real Time RT-PCR was used to quantify BRSV load in BAL fluids of four experimentally infected calves for 14 days. The high sensitivity, rapidity and reproducibility of the BRSV Real Time RT-PCR make this method suitable for diagnostic and for the evaluation of the efficiency of new vaccines. [less ▲]

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See detailIsolation of a glycoprotein E-deleted bovine herpesvirus type 1 strain in the field
Dispas, M.; Schynts, F.; Lemaire, Mylène et al

in Veterinary Record : Journal of the British Veterinary Association (2003), 153(7), 209-212

During a field trial to evaluate the efficacy of repeated vaccinations with bovine herpesvirus type 1 (BHV-1) marker vaccines, a glycoprotein E (gE)-negative BHV-1 strain was isolated from the nasal ... [more ▼]

During a field trial to evaluate the efficacy of repeated vaccinations with bovine herpesvirus type 1 (BHV-1) marker vaccines, a glycoprotein E (gE)-negative BHV-1 strain was isolated from the nasal secretions of two cows, eight months after vaccination with a gE-negative live-attenuated vaccine, initially given intranasally, then intramuscularly. The strain isolated was characterised using immunofluorescence, restriction analysis and PCR. All the techniques used identified the isolated virus as a gE-negative BHV-1 phenotypically and genotypically identical to the Za strain used as a control. [less ▲]

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See detailDiversity among Bovine Pestiviruses
Hamers, C.; Dehan, Pierre ULg; Couvreur, B. et al

in Veterinary Journal (2001), 161(2), 112-22

Bovine viral diarrhoea virus (BVDV) isolates are characterized by an important genetic, antigenic and pathogenic diversity. The emergence of new hypervirulent BVDV strains in North America has provided ... [more ▼]

Bovine viral diarrhoea virus (BVDV) isolates are characterized by an important genetic, antigenic and pathogenic diversity. The emergence of new hypervirulent BVDV strains in North America has provided clear evidence of pathogenic differences between BVDV strains. The origin of BVDV diversity is related to high mutation rate occurring in RNA viruses but the consequences of mutations obviously depend on the genes which are involved. Mutations in genes encoding for structural proteins of immunological importance may have practical implications.Knowledge of BVDV diversity is important for understanding the wide variety of pathogenesis of diseases caused by the virus, for monitoring the epidemiology of the different types and for the design of optimum laboratory tests and vaccines.This review focuses on the origin and consequences of BVDV diversity with regard to pathogenesis, biotypes, and antigenic and genetic variations. [less ▲]

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See detailAvancées récentes en biologie moléculaire du virus de la diarrhée virale bovine
Dehan, Pierre ULg; Hamers, C.; Couvreur, B. et al

in Annales de Médecine Vétérinaire (2001), 145

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See detailDifferences in Experimental Virulence of Bovine Viral Diarrhoea Viral Strains Isolated from Haemorrhagic Syndromes
Hamers, C.; Couvreur, B.; Dehan, Pierre ULg et al

in Veterinary Journal (2000), 160(3), 250-8

In the late 1980s, a new hypervirulent and epidemic form of bovine viral diarrhoea virus (BVDV) infection appeared in North America. A similar but sporadic syndrome was later reported in Europe. To ... [more ▼]

In the late 1980s, a new hypervirulent and epidemic form of bovine viral diarrhoea virus (BVDV) infection appeared in North America. A similar but sporadic syndrome was later reported in Europe. To compare the pathogenic characters of the North American and European hypervirulent strains, we inoculated BVDV naive calves with BVDV strains isolated from haemorrhagic syndromes originating in Belgium, France and the USA. The experimental procedure comprised daily clinical examination and measurement of blood and virological parameters.The American BVD890/256 strain induced severe thrombocytopaenia, profuse diarrhoea and pneumonia in all calves, indicating that hypervirulent BVDV could be the primary infectious agent of pneumonia. Interestingly, a strong correlation was observed between the intense viraemia and a decreased platelet count. None of the European strains tested induced significant pathological signs, although isolated from cases presenting haemorrhagic syndrome. [less ▲]

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See detailNatural case of bovine herpesvirus 1 meningoencephalitis in an adult cow.
Roels, S.; Charlier, G.; Letellier, C. et al

in Veterinary Record : Journal of the British Veterinary Association (2000), 146(20), 586-8

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See detailBiological-Activity Of Fenoxycarb Against Sitophilus-Zeamais Motsch (Coleoptera, Curculionidae)
Letellier, C.; Haubruge, Eric ULg; Gaspar, Charles ULg

in Journal of Stored Products Research (1995), 31(1),

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See detailControle et dynamique des populations de Sitophylus granarius (L.): mise au point d'un systeme pilote de silos experimentaux.
Letellier, C.; Haubruge, Eric ULg; Gaspar, Charles ULg

in Mededelingen van de Faculteit Landbouwkundige en Toegepaste Biologische Wetenschappen (Rijksuniversiteit te Gent) (1994), 59(2a),

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See detailImportance des insectes ravageurs des cereales stockees en Belgique.
Letellier, C.; Haubruge, Eric ULg; Gaspar, Charles ULg

in Parasitica (1994), 50(1-2),

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See detailExpression Of The Bovine Leukemia-Virus Transactivator Protein-P34 By A Recombinant Vaccinia Virus
Willems, Luc ULg; Letellier, C.; Gonze, M. et al

in Veterinary Immunology and Immunopathology (1989), 22(3),

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See detailExpression At The Cell-Surface Of Native Fusion Protein Of The Newcastle-Disease Virus (Ndv) Strain Italien From Cloned Cdna
Espion, D.; Dehenau, S.; Letellier, C. et al

in Archives of Virology (1987), 95(1-2), 79-95

A cDNA library was constructed with poly(A)+-mRNAs from NDV-Italien infected BHK-21 cells. A clone, that hybridized to the F gene mRNA, was sequenced. A long open reading frame encodes for a protein of ... [more ▼]

A cDNA library was constructed with poly(A)+-mRNAs from NDV-Italien infected BHK-21 cells. A clone, that hybridized to the F gene mRNA, was sequenced. A long open reading frame encodes for a protein of 553 amino acids, with a calculated molecular weight of 59,153, consisting of twelve cysteine residues and six potential glycosylation sites. The protein sequence contains a hydrophobic region at the N-terminus of F1 and a presumptive long transmembrane fragment near the C-terminus. Comparison of the F proteins from NDV strains Italien and Australia-Victoria shows that the sequences are very similar, with conservation of most cysteine residues and of the potential glycosylation sites. The F coding sequence was inserted into the genome of vaccinia virus under the control of vaccinia P7.5 transcriptional regulatory sequences. Expression of F protein was demonstrated by indirect immunofluorescence with five anti-F monoclonal antibodies known to react with conformational epitopes. [less ▲]

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