References of "Leprince, Pierre"
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See detailThe role of protein modifications in senescence of freeze-dried Acetobacter senegalensis during storage
Shafiei, Rasoul ULg; Zarmehrkhorshid, Raziyeh ULg; Bentaib, Azeddine ULg et al

in Microbial Cell Factories (2014)

Background Loss of viability is one of the most important problems during starter culture production. Previous research has mostly focused on the production process of bacterial starters, but there are ... [more ▼]

Background Loss of viability is one of the most important problems during starter culture production. Previous research has mostly focused on the production process of bacterial starters, but there are few studies about cellular protein deterioration causing cell defectiveness during storage. In the present study, we investigated the influence of storage temperature (−21, 4, 35°C) on the cellular protein modifications which may contribute to the senescence of freeze-dried Acetobacter senegalensis. Results Heterogeneous populations composed of culturable cells, viable but non-culturable cells (VBNC) and dead cells were generated when freeze-dried cells were kept at −21 and 4°C for 12 months whereas higher storage temperature (35°C) mainly caused death of the cells. The analysis of stored cell proteome by 2D-DiGE demonstrated a modified pattern of protein profile for cell kept at 4 and 35°C due to the formation of protein spot trains and shift of Isoelectric point (pI). Quantification of carbonylated protein by ELISA showed that the cells stored at 4 and 35°C had higher carbonylated protein contents than fresh cells. 2D-DiGE followed by Western blotting also confirmed the carbonylation of cellular proteins involved in translation process and energy generation. The auto-fluorescent feature of cells kept at 35°C increased significantly which may be an indication of protein glycation during storage. In addition, the percentage of cellular unsaturated fatty acid and the solubility of cellular proteins decreased upon storage of cells at higher temperature suggesting that peroxidation of fatty acids and possibly protein lipidation and oxidation occurred. Conclusions High storage temperature induces some deteriorative reactions such as protein oxidation, lipidation and glycation which may cause further protein modifications like pI-shift, and protein insolubility. These modifications can partly account for the changes in cell viability. It can also be deduced that even moderate carbonylation of some critical cellular proteins (like ribosomal proteins) may lead to VBNC formation or death of freeze-dried bacteria. Moreover, it seems that other mechanisms of biomolecule deterioration preceding protein carbonylation lead to VBNC formation under very low storage temperature. [less ▲]

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See detailThe small Heat Shock Proteins (HSPs) response following eccentric exercise
Hody, Stéphanie ULg; Simonet, Arnaud; Lacrosse, Zoé ULg et al

in Abstract Book GIGA Day 2014 (2014, January 27)

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See detailComprehensive plasma profiling for the characterization of graft-versus-host disease biomarkers
De Bock, Muriel; BEGUIN, Yves ULg; Leprince, Pierre ULg et al

in Talanta (2014), 125

Acutegraft-versus-hostdisease(aGVHD)remainsalife-threateningcomplicationofhematopoieticstem cell transplantation(HSCT)thereforelimitingitsapplication.TooptimizethemanagementofaGVHDand reduce therapy ... [more ▼]

Acutegraft-versus-hostdisease(aGVHD)remainsalife-threateningcomplicationofhematopoieticstem cell transplantation(HSCT)thereforelimitingitsapplication.TooptimizethemanagementofaGVHDand reduce therapy-relatedtoxicity,earlyspecific markersareneeded.Themainobjectiveofthisstudywas to uncoverdiagnosticbiomarkersbycomparingplasmaproteinprofiles ofpatientsatthetimeofacute GVHDdiagnosiswiththoseofpatientsundergoingHSCTwithoutaGVHD.Additionalanalysisofsamples taken 15daysbeforeaGVHDdiagnosiswasalsoperformedtoevaluatethepotentialofournewly discoveredbiomarkersforearlydiagnosis.Togetcomplementaryinformationfromplasmasamples, we usedthreedifferentproteomicapproaches,namely2D-DIGE,SELDI-TOF-MSand2D-LC-MSE. Weidentified andconfirmed bythemeansofindependenttechniques,thedifferentialexpression of severalproteinsindicatingsignificantly increasedinflammation responseanddisturbanceinthe coagulation cascade.Thevariationoftheseproteinswasalreadyobserved15daysbeforeGVHD diagnosis, suggestingthepotentialearlydetectionofthediseasebeforesymptomsappearance. Finally,logisticregressionanalysisdeterminedacompositebiomarkerpanelcomprising fibrinogen, fragment of fibrinogenbetachain,SAA,prothrombinfragments,apolipoproteinA1andhepcidinthat optimallydiscriminatedpatientswithandwithoutGVHD.Theareaunderthereceiveroperating characteristiccurvedistinguishingthese2groupswas0.95. [less ▲]

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See detailIsocinétisme et douleurs musculaires d’apparition retardée
Hody, Stéphanie ULg; ROGISTER, Bernard ULg; Leprince, Pierre ULg et al

in Movement & Sport Sciences - Science & Motricité (2014)

The curative and preventive efficiency of the isokinetic exercise, especially of the eccentric contraction, has been well demonstrated. However, intense or unusual eccentric exercise is known to induce ... [more ▼]

The curative and preventive efficiency of the isokinetic exercise, especially of the eccentric contraction, has been well demonstrated. However, intense or unusual eccentric exercise is known to induce muscle damage associated with delayed-onset muscle soreness (DOMS) and prolonged functional deficits. These negative consequences can frequently disturb the progress of re-education or training programmes. Since they can affect athletic performance and increase the risk of musculo-skeletal injuries, the structuro-functional alterations associated with DOMS may also be problematic in athletes. Therefore, to optimize the benefits of the eccentric work while avoiding muscle damage and occurrence of DOMS should represent a major objective for the practitioners. To date, the only systematic intervention that brings muscle protection against DOMS consists of performing repeated eccentric sessions at submaximal intensity. Besides its clinical use, isokinetic constitutes an interesting model to generate and investigate the DOMS phenomenon. The original association of eccentric injuring protocols with new emerging techniques of molecular biology appears to be a promising strategy to better understand the cellular and molecular mechanisms underlying exercise-induced muscle damage. Such data would provide better guidelines for prevention or treatment practice. [less ▲]

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See detailInactivation of genes coding for mitochondrial Nd7 and Nd9 complex I subunits in Chlamydomonas reinhardtii. Impact of complex I loss on respiration and energetic metabolism.
Massoz, Simon; Larosa, Véronique ULg; Plancke, Charlotte et al

in Mitochondrion (2013)

In Chlamydomonas, unlike in flowering plants, genes coding for Nd7 (NAD7/49kDa) and Nd9 (NAD9/30kDa) core subunits of mitochondrial respiratory-chain complex I are nucleus-encoded. Both genes possess all ... [more ▼]

In Chlamydomonas, unlike in flowering plants, genes coding for Nd7 (NAD7/49kDa) and Nd9 (NAD9/30kDa) core subunits of mitochondrial respiratory-chain complex I are nucleus-encoded. Both genes possess all the features that facilitate their expression and proper import of the polypeptides in mitochondria. By inactivating their expression by RNA interference or insertional mutagenesis, we show that both subunits are required for complex I assembly and activity. Inactivation of complex I impairs the cell growth rate, reduces the respiratory rate, leads to lower intracellular ROS production and lower expression of ROS scavenging enzymes, and is associated to a diminished capacity to concentrate CO2 without compromising photosynthetic capacity. [less ▲]

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See detailProteomic Analysis of the Reproductive Organs of the Hermaphroditic Gastropod Lymnea stagnalis Exposed to Different Endocrine Disrupting Chemicals
Giusti, Arnaud ULg; Leprince, Pierre ULg; Mazzucchelli, Gabriel ULg et al

in PLoS ONE (2013), 8(11), 81086

Many studies have reported perturbations of mollusc reproduction following exposure to low concentrations (ng/L range) of endocrine disrupting chemicals (EDCs). However, the mechanisms of action of these ... [more ▼]

Many studies have reported perturbations of mollusc reproduction following exposure to low concentrations (ng/L range) of endocrine disrupting chemicals (EDCs). However, the mechanisms of action of these molecules on molluscs are still poorly understood. Investigation of the modifications of protein expression in organisms exposed to chemicals using proteomic methods can provide a broader and more comprehensive understanding of adverse impacts of pollution on organisms than conventional biochemical biomarkers (e.g., heat-shock proteins, metallothioneins, GST, EROD). In this study we have investigated the impacts of four chemicals, which exhibit different endocrine disrupting properties in vertebrates, on the proteome of the hermaphroditic freshwater pulmonate gastropod Lymnaea stagnalis after 21 days of exposure. Testosterone, tributyltin, chlordecone and cyproterone acetate were chosen as tested compounds as they can induce adverse effects on the reproduction of this snail. The 2D-DIGE method was used to identify proteins whose expression was affected by these compounds. In addition to modifying the expression of proteins involved in the structure and function of the cytoskeleton, chemicals had impacts on the expression of proteins involved in the reproduction of L. stagnalis. Exposure to 19.2 μg/L of chlordecone increased the abundance of ovipostatin, a peptide transmitted during mating through seminal fluid, which reduces oviposition in this species. The expression of yolk ferritin, the vitellogenin equivalent in L. stagnalis, was reduced after exposure to 94.2 ng Sn/L of tributyltin. The identification of yolk ferritin and the modification of its expression in snails exposed to chemicals were refined using western blot analysis. Our results showed that the tested compounds influenced the abundance of yolk ferritin in the reproductive organs. Alteration in proteins involved in reproductive pathways (e.g., ovipostatin and yolk ferritin) could constitute relevant evidence of interaction of EDCs with reproductive pathways that are under the control of the endocrine system of L. stagnalis. [less ▲]

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See detailProteomic study of lumbar spinal cord after quadricipital eccentric exercise
Lacrosse, Zoé ULg; Hody, Stéphanie ULg; Leprince, Pierre ULg et al

Poster (2013, September 12)

Eccentric muscle contractions are characterized by an increase of muscle tension as it lengthens (slowering movements). Unaccustomed or intense eccentric exercise causes “Delayed-Onset Muscle Soreness” ... [more ▼]

Eccentric muscle contractions are characterized by an increase of muscle tension as it lengthens (slowering movements). Unaccustomed or intense eccentric exercise causes “Delayed-Onset Muscle Soreness” (DOMS). DOMS include muscle pain that appears 24 to 72 hours after exercise, but also stiffness, edema and muscle proteins release in plasma as a hallmark of muscle fibers injuries. The only systematic intervention that brings a muscle protection against DOMS is to realize submaximal eccentric contractions with a progressively increased intensity. The mechanism of this protection, called the “Repeated Bout Effect” (RBE), is not understood. However, it is likely explained by cellular, mechanical and neural theories [Scand.J.Med.&Sci.Sports, 13, 88, 2003]. The objective of this study is to better understand which neural signal is released in the muscle synapse and which brings protection by RBE. Male adult mice (C57BL6) were randomly divided into downhill running (DHR), uphill running (UHR) and untrained control (CONT) groups (n=4/group). DHR group is characterized by eccentric contractions of the quadriceps while UHR is concerned by concentric contractions. Running groups performed a warm-up of ten minutes followed by an interval exercise on an inclined treadmill at a velocity of 20cm/s. The latter consisted of running 18 bouts of 5 minutes interspersed with a 2 minutes rest. Lumbar spinal cord was dissected 24h after the race. Nuclear and cytoplasmic proteins were separately extracted and subjected to a 2D-DIGE analysis coupled with mass spectrometry. We do not observe any cytoplasmic protein modification while in the nuclear extract, seven spots were more abundant in eccentric group and four in concentric group in comparison with control group. The mass spectrometry of these proteins reveals that they are implicated in axoplasmic transport. At 24 hours, too few proteins modifications were detected in lumbar spinal cord, maybe as a consequence of a too short period between race and euthanasia. Implication of axoplasmic transport comforts our starting hypothesis that nervous system is able to protect muscle during the RBE by a synthesis and then a synaptic release of molecules modifying the muscle physiology. [less ▲]

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See detailProteomic study of lumbar spinal cord after quadricipital eccentric exercise
Lacrosse, Zoé ULg; Lacrosse, Zoé ULg; Hody, Stéphanie ULg et al

in 17th EURON PhD meeting (2013, September)

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See detailThe susceptibility of the knee extensors to eccentric exercise-induced muscle damage is not affected by leg dominance but by exercise order .
Hody, Stéphanie ULg; Rogister, Bernard ULg; Leprince, Pierre ULg et al

in Clinical Physiology & Functional Imaging (2013), 33(5), 373-380

The aims of this study were first to compare the response of dominant and non-dominant legs to eccentric exercise and second, to examine whether there is an effect of exercise order on the magnitude of ... [more ▼]

The aims of this study were first to compare the response of dominant and non-dominant legs to eccentric exercise and second, to examine whether there is an effect of exercise order on the magnitude of symptoms associated with intense eccentric protocols. Eighteen young men performed 3 sets of 30 maximal eccentric isokinetic (60°.sec-1) contractions of the knee extensors (range of motion, ROM: 0°-100°, 0=full extension) using either dominant or nondominant leg. They repeated a similar eccentric bout using the contralateral leg six weeks later. The sequence of leg’s use was allocated to create equally balanced groups. Four indirect markers of muscle damage including subjective pain intensity, maximal isometric strength, muscle stiffness and plasma CK activity were measured before and 24 hours after exercise. All markers changed significantly following the eccentric bout performed either by dominant or non-dominant legs but no significant difference was observed between legs. Interestingly, the comparison between the first and second eccentric bouts revealed that muscle soreness (-42%, p<0.001), CK activity (-62%, p<0.05) and strength loss (-54%, p<0.01) were significantly lower after the second bout. This study suggests that leg dominance does not influence the magnitude of exercise-induced muscle damage and supports for the first time the existence of a contralateral protection against exercise-induced muscle damage in the lower limbs. [less ▲]

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See detailEffects of eccentrically and concentrically biased training on mouse muscle phenotype
Hody, Stéphanie ULg; Lacrosse, Zoé ULg; Leprince, Pierre ULg et al

in Medicine & Science in Sports & Exercise (2013), 45(8), 1460-1468

Introduction: The molecular adaptations specifically induced by different muscle contraction types have only been partially elucidated. We previously demonstrated that eccentric contractions in human ... [more ▼]

Introduction: The molecular adaptations specifically induced by different muscle contraction types have only been partially elucidated. We previously demonstrated that eccentric contractions in human quadriceps elicited proteome modifications that suggest a muscle fiber typology adaptation. We address this question in a more systematic way by examining here the effects of different running modes on the mouse muscle proteome and the muscle fiber typology. Methods: Male adult mice (C57BL6) were randomly divided into downhill running (DHR, quadricipital eccentrically biased contractions), uphill running (UHR, quadricipital concentrically biased contractions) and untrained control (CONT) groups. Running groups performed five training sessions on an inclined treadmill for 75 to 135 min/day and the quadriceps muscles were dissected 96hours after the last session. Muscle protein extracts of DHR and UHR groups (n=4/group) were subjected to a 2D-DIGE analysis coupled with mass spectrometry. The assessment of fiber type, size and number was performed on the rectus femoris of the three groups (n=6/group) using myosin heavy chain (MHC) immunohistochemistry. Results: In the proteomic analysis, eight spots identified as the fast MHC isoforms exhibited a lower abundance in DHR compared to UHR (p<0.05, t-test). In contrast, ATP synthase subunit α and tubulin β were more expressed in DHR (p<0.05). A significant higher proportion of type I and IIa fibers was found for DHR compared to UHR or CONT groups (p<0.05, one-way ANOVA). Conclusions: Our data suggest that the eccentrically biased contractions in mice induced specific adaptations in protein expression and muscle fiber composition which may reflect a more oxidative muscle phenotype. The differences in stress placed on the muscle between both trainings may be responsible for some unique adaptations resulting from the eccentrically biased training. [less ▲]

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See detailMuscle fatigue experienced during maximal eccentric exercise is predictive of the plasma creatine kinase (CK) response
Hody, Stéphanie ULg; Rogister, Bernard ULg; Leprince, Pierre ULg et al

in Scandinavian Journal of Medicine & Science in Sports (2013), 23(4), 501-7

Unaccustomed eccentric exercise may cause skeletal muscle damage with an increase in plasma creatine kinase (CK) activity. Although the wide variability among individuals in CK response to standardized ... [more ▼]

Unaccustomed eccentric exercise may cause skeletal muscle damage with an increase in plasma creatine kinase (CK) activity. Although the wide variability among individuals in CK response to standardized lengthening contractions has been well described, the reasons underlying this phenomenon have not yet been understood. Therefore, this study investigated a possible correlation of the changes in muscle damage indirect markers after an eccentric exercise with the decline in muscle performance during the exercise. Twenty-seven healthy untrained male subjects performed three sets of 30 maximal isokinetic eccentric contractions of the knee extensors. The muscular work was recorded using an isokinetic dynamometer to assess muscle fatigue by means of various fatigue indices. Plasma CK activity, muscle soreness, and stiffness were measured before (pre) and one day after (post) exercise. The eccentric exercise bout induced significant changes of the three muscle damage indirect markers. Large intersubject variability was observed for all criteria measured. More interestingly, the log (CKpost/CKpre) and muscle stiffness appeared to be closely correlated with the relative work decrease (r = 0.84, r2 = 0.70 and r = 0.75, r2 = 0.56, respectively). This is the first study to propose that the muscle fatigue profile during maximal eccentric protocol could predict the magnitude of the symptoms associated with muscle damage in humans. [less ▲]

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See detailThe role of Bergmann glial cells in cerebellar development
Leprince, Pierre ULg

in Cancer & Metabolism (2013), 1

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See detailEffects of eccentric versus concentric training on mouse muscle phenotype
Hody, Stéphanie ULg; Lacrosse, Zoé ULg; Leprince, Pierre ULg et al

in Abstract Book of 10th Meeting of Belgian Society for Neuroscience (2013, May)

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See detailPrévention des courbatures musculaires en pratique sportive
Hody, Stéphanie ULg; Delvaux, François; Rodriguez de la Cruz, Carlos ULg et al

in Prévention des lésions musculo-squelettiques chez le sportif (2013)

L’exercice excentrique, lorsqu’il est réalisé de manière intense et/ou inhabituelle, est réputé être à l’origine de diverses sensations de courbatures d’apparition retardée. Ces douleurs musculaires ... [more ▼]

L’exercice excentrique, lorsqu’il est réalisé de manière intense et/ou inhabituelle, est réputé être à l’origine de diverses sensations de courbatures d’apparition retardée. Ces douleurs musculaires, appelées DOMS pour Delayed-Onset Muscle Soreness, s’accompagnent de déficits structuro-fonctionnels pouvant persister pendant plusieurs jours. Bien qu’il disparaisse spontanément après quelques jours de récupération, le phénomène des DOMS reste problématique chez le sportif en raison des conséquences néfastes associées : perturbation de l’entraînement sportif, diminution de la performance athlétique et majoration du risque de lésions véritables en cas de poursuite d’activités sportives. Il apparaît donc essentiel, pour un sportif soumis à un entraînement excentrique, d’échapper aux DOMS. La mise au point d’approches susceptibles de réduire les conséquences néfastes des DOMS a constitué un objectif prioritaire de nombreuses études. Ainsi, les premiers essais ont été basés sur des stratégies nutritionnelles ou pharmacologiques ainsi que sur des procédés cliniques. Bien que certains procédés tels que le massage ou les apports nutritionnels puissent influencer de manière positive l’un ou l’autre symptôme, ceux-ci ne semblent pas capables d’agir sur le phénomène des DOMS dans sa globalité. Actuellement, la seule démarche préventive réellement efficace reste la réalisation d’un entraînement excentrique à intensité sous-maximale progressivement intensifiées. [less ▲]

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See detailMesenchymal stem cells and neural crest stem cells from adult bone marrow: characterization of their surprising similarities and differences.
Wislet, Sabine ULg; Laudet, Emerence ULg; Neirinckx, Virginie ULg et al

in Cellular and Molecular Life Sciences : CMLS (2012), 69(15), 2593-2608

The generation of neuronal cells from stem cells obtained from adult bone marrow is of significant clinical interest in order to design new cell therapy protocols for several neurological disorders. The ... [more ▼]

The generation of neuronal cells from stem cells obtained from adult bone marrow is of significant clinical interest in order to design new cell therapy protocols for several neurological disorders. The recent identification in adult bone marrow of stem cells derived from the neural crest stem cells (NCSC) might explain the neuronal phenotypic plasticity shown by bone marrow cells. However, little information is available about the nature of these cells compared to mesenchymal stem cells (MSC), including their similarities and differences. In this paper, using transcriptomic as well as proteomic technologies, we compared NCSC to MSC and stromal nestin-positive cells, all of them isolated from adult bone marrow. We demonstrated that the nestin-positive cell population, which was the first to be described as able to differentiate into functional neurons, was a mixed population of NCSC and MSC. More interestingly, we demonstrated that MSC shared with NCSC the same ability to truly differentiate into Tuj1-positive cells when co-cultivated with paraformaldehyde-fixed cerebellar granule neurons. Altogether, those results suggest that both NCSC and MSC can be considered as important tools for cellular therapies in order to replace neurons in various neurological diseases. [less ▲]

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See detailProteomic comparison of high and low responders to eccentric exercise
Hody, Stéphanie ULg; Croisier, Jean-Louis ULg; Leprince, Pierre ULg et al

in Meeusen, R.; Duchateau, J.; Roelands, B. (Eds.) et al Book of Abstracts of the 17th annual Congress of the ECSS (2012, July)

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See detailMuscle fatigue experienced during maximal eccentric exercise is predictive of the plasma creatine kinase (CK) response
Hody, Stéphanie ULg; Rogister, Bernard ULg; Leprince, Pierre ULg et al

in Meeusen, R.; Duchateau, J.; Roelands, B. (Eds.) et al Book of Abstracts of the 17th annual Congress of the ECSS (2012, July)

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See detailIs there a cold shock response in the Antarctic psychrophile Pseudoalteromonas haloplanktis?
Piette, Florence ULg; Leprince, Pierre ULg; Feller, Georges ULg

in Extremophiles : Life Under Extreme Conditions (2012), 16

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See detailIdentification of protein networks involved in the disease course of experimental autoimmune encephalomyelitis, an animal model of multiple sclerosis.
Vanheel, Annelies; Daniels, Ruth; Plaisance, Stephane et al

in PLoS ONE (2012), 7(4), 35544

A more detailed insight into disease mechanisms of multiple sclerosis (MS) is crucial for the development of new and more effective therapies. MS is a chronic inflammatory autoimmune disease of the ... [more ▼]

A more detailed insight into disease mechanisms of multiple sclerosis (MS) is crucial for the development of new and more effective therapies. MS is a chronic inflammatory autoimmune disease of the central nervous system. The aim of this study is to identify novel disease associated proteins involved in the development of inflammatory brain lesions, to help unravel underlying disease processes. Brainstem proteins were obtained from rats with MBP induced acute experimental autoimmune encephalomyelitis (EAE), a well characterized disease model of MS. Samples were collected at different time points: just before onset of symptoms, at the top of the disease and following recovery. To analyze changes in the brainstem proteome during the disease course, a quantitative proteomics study was performed using two-dimensional difference in-gel electrophoresis (2D-DIGE) followed by mass spectrometry. We identified 75 unique proteins in 92 spots with a significant abundance difference between the experimental groups. To find disease-related networks, these regulated proteins were mapped to existing biological networks by Ingenuity Pathway Analysis (IPA). The analysis revealed that 70% of these proteins have been described to take part in neurological disease. Furthermore, some focus networks were created by IPA. These networks suggest an integrated regulation of the identified proteins with the addition of some putative regulators. Post-synaptic density protein 95 (DLG4), a key player in neuronal signalling and calcium-activated potassium channel alpha 1 (KCNMA1), involved in neurotransmitter release, are 2 putative regulators connecting 64% of the identified proteins. Functional blocking of the KCNMA1 in macrophages was able to alter myelin phagocytosis, a disease mechanism highly involved in EAE and MS pathology. Quantitative analysis of differentially expressed brainstem proteins in an animal model of MS is a first step to identify disease-associated proteins and networks that warrant further research to study their actual contribution to disease pathology. [less ▲]

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See detailHuman muscle proteome modifications after acute or repeated eccentric exercises
Hody, Stéphanie ULg; Leprince, Pierre ULg; Sergeant, K. et al

in Medicine & Science in Sports & Exercise (2011), 43(12), 2281-2296

INTRODUCTION:: DOMS (Delayed-Onset Muscle Soreness), a condition triggered by eccentric exercise, affects muscle cells at a biochemical level in a poorly understood fashion. The objective of the present ... [more ▼]

INTRODUCTION:: DOMS (Delayed-Onset Muscle Soreness), a condition triggered by eccentric exercise, affects muscle cells at a biochemical level in a poorly understood fashion. The objective of the present study was to examine human muscle proteome modifications induced by strenuous eccentric exercises following a specific training aimed to prevent DOMS. METHODS:: Biopsy of the rectus femoris were taken from healthy human volunteers in three successive conditions: (1) at rest, (2) 24 hours after an injuring exercise protocol consisting of 3 series of 30 maximal contractions of the quadriceps on an isokinetic dynamometer, (3) 24 hours after a similar exercise bout preceded either by 5 eccentric training sessions, or no training. RESULTS:: Muscle damage was assessed before and 1 day after each maximal eccentric test by comparing three indirect markers: plasma activity of creatine kinase (CK), muscle stiffness and subjective pain intensity. Compared to the first eccentric test, those markers were reduced after the second test and further reduced if this second test followed the eccentric training, thus confirming the protective effect of such training. Muscle protein extracts were subjected to a 2D-DIGE proteomic analysis coupled with MALDI-TOF-MS protein identification. Surprisingly, we observed that myosin heavy chains decreased after the first eccentric test, and were reduced further with other contractile proteins after the second test. Furthermore, the expression of several glycolytic enzymes decreased only after the second test that was preceded by a specific training. CONCLUSION:: These findings suggest that the eccentric training resulted in a switch to oxidative metabolism, which may be associated with protection from DOMS. [less ▲]

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