   A New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Specific Surface Chemistry Applied onto Titration Wel; ; Zorzi, Willy  et alin Biosensors (2012), 2(4), 433-447 This work illustrates the enhancement of the sensitivity of the ELISA titration for recombinant human and native prion proteins, while reducing other non-specific adsorptions that could increase the ... [more ▼] This work illustrates the enhancement of the sensitivity of the ELISA titration for recombinant human and native prion proteins, while reducing other non-specific adsorptions that could increase the background signal and lead to a low sensitivity and false positives. It is achieved thanks to the association of plasma chemistry and coating with different amphiphilic molecules bearing either ionic charges and/or long hydrocarbon chains. The treated support by 3-butenylamine hydrochloride improves the signal detection of recombinant protein, while surface modification with the 3,7-dimethylocta-2,6-dien-1-diamine (geranylamine) enhances the sensitivity of the native protein. Beside the surface chemistry effect, these different results are associated with protein conformation. [less ▲] Detailed reference viewed: 10 (0 ULg) Increasing the Detection Limit of the Parkinson Disorder through a Specific Surface Chemistry Applied onto Inner Surface of the Titration Well; ; Zorzi, Willy et alin Journal of Functional Biomaterials (2012), 2 The main objective of this paper was to illustrate the enhancement of the sensitivity of ELISA titration for neurodegenerative proteins by reducing nonspecific adsorptions that could lead to false ... [more ▼] The main objective of this paper was to illustrate the enhancement of the sensitivity of ELISA titration for neurodegenerative proteins by reducing nonspecific adsorptions that could lead to false positives. This goal was obtained thanks to the association of plasma and wet chemistries applied to the inner surface of the titration well. The polypropylene surface was plasma-activated and then, dip-coated with different amphiphilic molecules. These molecules have more or less long hydrocarbon chains and may be charged. The modified surfaces were characterized in terms of hydrophilic—phobic character, surface chemical groups and topography. Finally, the coated wells were tested during the ELISA titration of the specific antibody capture of the α-synuclein protein. The highest sensitivity is obtained with polar (Θ = 35°), negatively charged and smooth inner surface. [less ▲] Detailed reference viewed: 7 (1 ULg)How to control the recombinant prion protein adhesion for successful storage through modification of surface properties Biointerphases; ; et al in Biointerphases (2012) Detailed reference viewed: 6 (0 ULg)Non-Adhesive Behavior of New Nanostructured PNIPAM Surfaces Towards Specific Neurodegenerative Proteins: Application to Storage and Titration of Tau Proteins; ; et al in Macromolecular Bioscience (2012) Detailed reference viewed: 8 (0 ULg) Are the interactions between recombinant prion protein and polymeric surfaces related to the hydrophilic and hydrophobic balance ?; ; et al in Macromolecular Bioscience (2012), In press In press Detailed reference viewed: 9 (0 ULg)Are the interactions between neurodegenerative proteins and polymeric surfaces related to the hydrophilic and hydrophobic balance ?; ; et al in Macromolecular Bioscience (2012), In press In press Detailed reference viewed: 3 (0 ULg)Improvement of the Detection of Neurodegenerative Alzheimer's Disease through a Specific Surface Chemistry Applied onto the Inner Surface of the Titration Well.; ; Zorzi, Willy et alin Journal of Biomaterials Science. Polymer Edition (2011) Detailed reference viewed: 11 (3 ULg)Study of the Adhesion of Neurodegenerative Proteins on Plasma-Modified and Coated Polypropylene Surfaces.; ; et al in Journal of Biomaterials Science. Polymer Edition (2011) Detailed reference viewed: 21 (3 ULg) |
||
