References of "Ledent, Philippe"
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See detailDevelopment of an Environmental Information System for Odour using Citizen and Technology Innovative Sensors and Advanced Modelling
Uhrner, Ulrich; Grosso, gioavanna; Romain, Anne-Claude ULg et al

in CEUR Workshop Proceedings (2014), 1322

The challenge of OMNISCIENTIS is to develop a community based odour monitoring and information system to mitigate odour annoyance and to foster citizens’ participation in environmental governance. The ... [more ▼]

The challenge of OMNISCIENTIS is to develop a community based odour monitoring and information system to mitigate odour annoyance and to foster citizens’ participation in environmental governance. The core is an information system collecting various data of odour emissions obtained by electronic noses and other sensors, meteorological conditions and observations by citizens acting as human sensors. A specific odour dispersion model is developed to use all this information and provide immediate feedback to all stakeholders. This work presents the architecture of the environmental information system, some first results from odour monitoring and model development and validation. [less ▲]

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See detailDesign, implementation, and test of a digitally-controlled electrical power supply for the OUFTI-1 nanosatellite
Kolodziej, Marie; Ledent, Philippe; Thirion, Pierre et al

Poster (2010)

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See detailOUFTI-1: New Student Nano-Satellite at ULg
Galli, Stefania; Ledent, Philippe; Pisane, Jonathan ULg et al

Scientific conference (2008)

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See detailOUFTI-1: The CubeSat developed at the University of Liège, Belgium
Galli, Stefania; Pisane, Jonathan ULg; Ledent, Philippe et al

Conference (2008)

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See detailUnexpected Influence of a C-Terminal-Fused His-Tag on the Processing of an Enzyme and on the Kinetic and Folding Parameters
Ledent, Philippe; Duez, Colette ULg; Vanhove, Marc et al

in FEBS Letters (1997), 413(2), 194-196

The addition of a poly-His C-terminal extension, designed to facilitate the purification of the protein, to the beta-lactamase of a thermophilic Bacillus licheniformis strain modified the site of action ... [more ▼]

The addition of a poly-His C-terminal extension, designed to facilitate the purification of the protein, to the beta-lactamase of a thermophilic Bacillus licheniformis strain modified the site of action of the signal peptidase. This resulted in the secretion of a protein with a different N-terminus, showing that this type of protein engineering might not always be as 'neutral' as generally assumed. (C) 1997 Federation of European Biochemical Societies. [less ▲]

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See detailKinetic Study of Interaction between Brl 42715, Beta-Lactamases, and D-Alanyl-D-Alanine Peptidases
Matagne, André ULg; Ledent, Philippe; Monnaie, Didier et al

in Antimicrobial Agents and Chemotherapy (1995), 39(1), 227-31

A detailed kinetic study of the interactions between BRL 42715, a beta-lactamase-inhibiting penem, and various beta-lactamases (EC 3.5.2.6) and D-alanyl-D-alanine peptidases (DD-peptidases, EC 3.4.16.4 ... [more ▼]

A detailed kinetic study of the interactions between BRL 42715, a beta-lactamase-inhibiting penem, and various beta-lactamases (EC 3.5.2.6) and D-alanyl-D-alanine peptidases (DD-peptidases, EC 3.4.16.4) is presented. The compound was a very efficient inactivator of all active-site serine beta-lactamases but was hydrolyzed by the class B, Zn(2+)-containing enzymes, with very different kcat values. Inactivation of the Streptomyces sp. strain R61 extracellular DD-peptidase was not observed, and the Actinomadura sp. strain R39 DD-peptidase exhibited a low level of sensitivity to the compound. [less ▲]

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See detailModelling of Accessibility Fields
Donnay, Jean-Paul ULg; Ledent, Philippe

in Proceeding of the First Joint European Conference on Geographical Information (1995)

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See detailA comparative study of class-D beta-lactamases.
Ledent, Philippe; Raquet, X; Joris, Bernard ULg et al

in Biochemical Journal (1993), 292 ( Pt 2)

Three class-D beta-lactamases (OXA2, OXA1 and PSE2) were produced and purified to protein homogeneity. 6 beta-Iodopenicillanate inactivated the OXA2 enzyme without detectable turnover. Labelling of the ... [more ▼]

Three class-D beta-lactamases (OXA2, OXA1 and PSE2) were produced and purified to protein homogeneity. 6 beta-Iodopenicillanate inactivated the OXA2 enzyme without detectable turnover. Labelling of the same beta-lactamase with 6 beta-iodo[3H]penicillanate allowed the identification of Ser-70 as the active-site serine residue. In agreement with previous reports, the apparent M(r) of the OXA2 enzyme as determined by molecular-sieve filtration, was significantly higher than that deduced from the gene sequence, but this was not due to an equilibrium between a monomer and a dimer. The heterogeneity of the OXA2 beta-lactamase on ion-exchange chromatography contrasted with the similarity of the catalytic properties of the various forms. A first overview of the enzymic properties of the three 'oxacillinases' is presented. With the OXA2 enzyme, 'burst' kinetics, implying branched pathways, seemed to prevail with many substrates. [less ▲]

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See detailMechanism of action of β-lactamases and DD-peptidases
Frère, Jean-Marie ULg; Joris, Bernard ULg; Jacob, Françoise et al

in Pandit, U.K.; Alderweireldt, F.C. (Eds.) Bioorganic Chemistry in Healthcare and Technology (1991)

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