References of "Lecrenier, Marie-Caroline"
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See detailA mass spectrometry method for sensitive, specific and simultaneous detection of bovine blood meal, blood products and milk products in compound feed
Lecrenier, Marie-Caroline ULiege; Planque, Mélanie; Dieu, Marc et al

in Food Chemistry (2017)

Feed sustainability is one of the biggest challenges for the next few years. Solutions have to be found that take feed quality and safety into account. Animal by-products are one valuable source of ... [more ▼]

Feed sustainability is one of the biggest challenges for the next few years. Solutions have to be found that take feed quality and safety into account. Animal by-products are one valuable source of proteins. However, since the bovine spongiform encephalopathy (BSE) crisis, their use has been strictly regulated. The objective of this study was to propose a routine, sensitive and specific method using ultra-high performance liquid chromatography coupled to tandem mass spectrometry for the detection of blood-derived products and milk powder in feed. Contaminated aquafeeds were analysed in order to evaluate the sensitivity and specificity of the method. This new method meets both selectivity and sensitivity (0.1% (w/w)) requirements imposed by the European Commission for animal proteins detection methods. It offers an innovative and complementary solution for the simultaneously identification of authorised and unauthorised animal by-products such as processed animal proteins (PAPs). [less ▲]

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See detailENTOFÔR project -From waste to resource
Lecrenier, Marie-Caroline ULiege; Fumière, Olivier; Berntssen, M.H.G. et al

Poster (2017, November)

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See detailUltra-High Performance Liquid Chromatography coupled to tandem Mass Spectrometry for the detection of animal by-products in feed
Lecrenier, Marie-Caroline ULiege; Planque, Mélanie; Dieu, Marc et al

in Book of abstracts of the 8th International Symposium on Recent advances in food analysis (2017, November)

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See detailSynchronous fluorescence spectroscopy for detecting blood meal and blood products in feed
Abbas, Ouissam; Lecrenier, Marie-Caroline ULiege; Baeten, Vincent

Poster (2017, September)

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See detailEffect of grinding on bone detection by light microscopy: Study results
Lecrenier, Marie-Caroline ULiege; Veys, Pascal; Planchon, Viviane et al

Conference (2017, June)

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See detailDetection of blood and milk in aquafeed using UHPLC MS/MS
Lecrenier, Marie-Caroline ULiege; Veys, Pascal; Fumière, Olivier et al

Conference (2017, April)

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See detailSpecific detection of blood derived products in animal feed using UPLC-MS/MS
Lecrenier, Marie-Caroline ULiege; Dieu, Marc; Veys, Pascal et al

Poster (2016, November)

Blood derived products are valuable animal products used in feed for their nutritional value and their positive effects on growth and health. Nevertheless, since the BSE crisis, their use is strictly ... [more ▼]

Blood derived products are valuable animal products used in feed for their nutritional value and their positive effects on growth and health. Nevertheless, since the BSE crisis, their use is strictly regulated. Blood meal and blood products (hemoglobin powder and plasma powder) of bovine origin are totally prohibited. Blood meal and blood products of porcine origin are authorised in aquafeed, whereas only blood products are allowed to be used in feed intended for other non-ruminant. The detection of the type of protein and the species of origin is therefore crucial to ensure feed safety. With the current official methods for the detection of PAPs, light microscopy and PCR, it is not always possible to specifically identify this type of protein source. The objective of our work was to set-up a routine mass spectrometry method for a qualitative detection of blood meal and hemoglobin powder of bovine and porcine origin in feed. The method was based on the detection of species-specific peptides biomarkers identified in a previous study by a non-targeted approach. All biomarkers were peptides of α and β hemoglobin subunits. Proteins were extracted using a TCA/acetone protein precipitation protocol followed by purification with a 2-D Clean-Up Kit (GE Healthcare, USA). After an in-solution trypsin digestion step, analyses were performed by liquid chromatography (Acquity system, Waters, UK) coupled with a triple quadrupole mass spectrometer (Xevo TQS, Waters, UK) with electrospray ionisation. The acquisition and processing of data was carried out by MassLynx software (v. 4.1, Waters) and multiple reaction monitoring (MRM) design was made using the open-source software Skyline (https://skyline.gs.washington.edu/labkey/project/home/software/Skyline/begin.view). Reference hemoglobin powder was used to select MRM transitions for each peptide biomarkers and to optimise their collision energy. Commercial feed material and compound feed containing or free from blood derived products were then analysed. Selected transitions were present in materials or feed known to contained blood derived product of the same origin and absent in the others. Artificially contaminated feed with various contamination levels were also analysed in order to evaluate the influence of matrix composition and to experimentally determine the limit of detection. A first estimation of the LOD was around 0.05 % w/w which was below the LOD imposed by the EC for animal proteins detection method. [less ▲]

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See detailBlood meal and blood products detection using Synchronous fluorescence spectroscopy
Lecrenier, Marie-Caroline ULiege; Abbas, Ouissam; Taira, Aurélien et al

Poster (2016, November)

In Europe, ruminant processed animal proteins (PAPs) and blood products are not allowed to be used in feed for farmed animal. In contrast, blood meal and blood products of porcine origin are both ... [more ▼]

In Europe, ruminant processed animal proteins (PAPs) and blood products are not allowed to be used in feed for farmed animal. In contrast, blood meal and blood products of porcine origin are both authorised in aqua feed, whereas only porcine blood products are allowed to be used in feed intended for other non-ruminants. Besides official methods (light microscopy and PCR), complementary methods are developed in order to refine the by-products identification. By-products derived from blood are one of these products for which additional information are needed. Indeed, these prohibited materials sometimes cannot be distinguished from those authorised (e.g. milk powder) using official methods. The aim of this work was to develop a fast and easy method to detect blood meal and blood products. This study was based on the detection of hemoglobin in animal feed by synchronous fluorescence spectroscopy (SFS). To achieve this goal, preliminary tests were carried out on reference material (hemoglobin and albumin purchased from Sigma Aldrich) in order to determine appropriate conditions (solvent) and parameters (offset values) for hemoglobin detection. Selected settings were then applied to analyse protein extracts of commercial feed material derived from blood. The results obtained on blood meal and blood products (hemoglobin powder and plasma powder) showed fluorescence spectral bands that characterise hemoglobin. In order to determine whether the method was fit for the purpose, real commercial compound feeds known to contain or to be free from blood products or blood meal were analysed. The Principal Component Analysis (PCA) applied to these spectra showed that it was possible to discriminate samples containing hemoglobin from those that do not contain. This result confirmed that SFS is a promising screening method for the detection of hemoglobin in animal feed. [less ▲]

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See detailDetection of blood derived products in feed using targeted triple-quadrupole MS
Lecrenier, Marie-Caroline ULiege; Dieu, Marc; Veys, Pascal et al

Conference (2016, September)

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See detailIdentification of specific bovine blood biomarkers with a non-targeted approach using HPLC ESI tandem mass spectrometry
Lecrenier, Marie-Caroline ULiege; Marbaix, Hélène; Dieu, Marc et al

in Food Chemistry (2016), 213

Animal by-products are valuable protein sources in animal nutrition. Among them are blood products and blood meal, which are used as high-quality material for their beneficial effects on growth and health ... [more ▼]

Animal by-products are valuable protein sources in animal nutrition. Among them are blood products and blood meal, which are used as high-quality material for their beneficial effects on growth and health. Within the framework of the feed ban relaxation, the development of complementary methods in order to refine the identification of processed animal proteins remains challenging. The aim of this study was to identify specific biomarkers that would allow the detection of bovine blood products and processed animal proteins using tandem mass spectrometry. Seventeen biomarkers were identified: nine peptides for bovine plasma powder; seven peptides for bovine haemoglobin powder, including six peptides for bovine blood meal; and one peptide for porcine blood. They were not detected in several commercial compound feed or feed materials, such as blood by-products of other animal origins, milk-derived products and fish meal. These biomarkers could be used for developing a species-specific and blood-specific detection method. [less ▲]

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See detailDéveloppement de méthodes complémentaires pour la détection des protéines animales: Focus sur la spectrométrie de masse
Lecrenier, Marie-Caroline ULiege; Veys, Pascal; Berben, Gilbert et al

Conference (2015, November)

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See detailInnovative methods for the determination of the taxonomic origin of processed animal proteins in feed.
Veys, Pascal; Ngo Njembe, M.; Lecrenier, Marie-Caroline ULiege et al

Poster (2015, November)

The use of animal by-products in feed depends on their nature defined by the type of tissue or body parts and the species of origin. Currently, the detection of unauthorised processed animal proteins ... [more ▼]

The use of animal by-products in feed depends on their nature defined by the type of tissue or body parts and the species of origin. Currently, the detection of unauthorised processed animal proteins (PAPs) is based on light microscopy and PCR methods. Light microscopy identifies structures on the basis of their morphology and enables identification of particles (such as bones, cartilages, muscle fibres,…) while PCR is able to detect and identify the presence of specific animal DNA in feed. Nevertheless, for some scenarios, even combined, these methods do not succeed in determining the taxonomic origin of the PAPs. A typical example is that of an aquafeed containing authorised porcine PAP together with dairy products: the analysis will conclude of the potential presence of ruminant PAP. Therefore, there is a need for developing methods allowing a taxonomic characterisation of visual structures such as bones fragments and muscle fibres. For the characterisation of bones, NIRM has yet demonstrated its potential. However the limitation of NIRM is when the presence of bones is reduced or absent. This study investigated the potential of NIRM for the determination of the taxonomic origin of muscle fibres. The NIRM was experimented on 2 porcine PAPs vs. 6 ruminant PAPs and 7 fishmeals all of industrial origin. Results showed that NIRM allows differentiating muscle fibres from different taxonomic origins: fish, ruminant and pig. In addition to this taxonomic classification, results also reveal differences inside taxonomic clusters of PAPs (e.g among different ruminant PAPs and porcine PAPs). The results obtained on this type of meals are promising and offer new perspectives. Tests on adulterated feeds need to be performed by NIRM prior to validation. [less ▲]

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See detailBovine blood biomarkers as a way of processed animal proteins detection in feedingstuffs
Lecrenier, Marie-Caroline ULiege; Marbaix, Hélène; Veys, Pascal et al

Poster (2015, November)

The prohibition of using animal by-products in feedingstuffs depends on two factors: their nature defined by the tissue/cell type and the species of origin, and on their destination (pets, fur animals or ... [more ▼]

The prohibition of using animal by-products in feedingstuffs depends on two factors: their nature defined by the tissue/cell type and the species of origin, and on their destination (pets, fur animals or other farmed animals). Proteomics is particularly well-suited to the purpose of PAPs detection as it is a tissue and species-specific method. The aim of this study was the identification and the selection of specific peptide biomarkers using tandem mass spectrometry for the detection of bovine blood products and blood meals in animal feed. Twenty-nine samples of blood meals and blood products (plasma or haemoglobin powder) of porcine, poultry and bovine origin as well as three milk products and two fish meals were analysed using a Q TOF mass spectrometer. Vegetal feed samples adulterated with 1% or 10% of bovine plasma powder, haemoglobin powder or blood meal were also analysed to evaluate the applicability of the method. Four proteins of interest were highlighted: Alpha-2-macroglobulin, apolipoprotein A-1, serotransferrin and haemoglobin (α and β chains). From these proteins, sixteen peptides were identified as potential bovine blood biomarkers in feedingstuffs. Nine of them could be used for the detection of plasma powder and seven of them for haemoglobin powder or blood meal. The evaluation of these peptides by a search against NCBInr database revealed that some of them could also be used to detect other ruminant bloods such as ovine or caprine ones. These preliminary results are promising. Efforts are now focused to improve the protocol in order to increase the sensitivity of the method as regards the selected proteins. [less ▲]

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See detailInnovative Method for the Detection of Bovine Blood Proteins in Feedingstuffs
Lecrenier, Marie-Caroline ULiege; Marbaix, Hélène; Veys, Pascal et al

Poster (2015, October)

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See detailMass spectrometry for the detection of blood meal/products
Lecrenier, Marie-Caroline ULiege; Veys, Pascal; Fumière, Olivier et al

Conference (2015, April)

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See detailInnovative method for the detection of bovine blood products in feedingstuffs
Lecrenier, Marie-Caroline ULiege; Marbaix, Hélène; Veys, Pascal et al

Poster (2014, October)

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See detailDetermination of the ruminant origin of bone particles using fluorescence in situ hybridization (FISH).
Lecrenier, Marie-Caroline ULiege; Ledoux, Quentin; Berben, Gilbert et al

in Scientific reports (2014), 4

Molecular biology techniques such as PCR constitute powerful tools for the determination of the taxonomic origin of bones. DNA degradation and contamination by exogenous DNA, however, jeopardise bone ... [more ▼]

Molecular biology techniques such as PCR constitute powerful tools for the determination of the taxonomic origin of bones. DNA degradation and contamination by exogenous DNA, however, jeopardise bone identification. Despite the vast array of techniques used to decontaminate bone fragments, the isolation and determination of bone DNA content are still problematic. Within the framework of the eradication of transmissible spongiform encephalopathies (including BSE, commonly known as "mad cow disease"), a fluorescence in situ hybridization (FISH) protocol was developed. Results from the described study showed that this method can be applied directly to bones without a demineralisation step and that it allows the identification of bovine and ruminant bones even after severe processing. The results also showed that the method is independent of exogenous contamination and that it is therefore entirely appropriate for this application. [less ▲]

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See detailFluorescence in situ Hybridization (FISH) for the taxonomic origin determination of processed animal proteins in Aquafeed
Lecrenier, Marie-Caroline ULiege; Ledoux, Quentin; Berben, Gilbert et al

Scientific conference (2014, June)

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See detailComplementary methods - last developments on FISH and Mass Spectrometry
Lecrenier, Marie-Caroline ULiege; Veys, Pascal; Berben, Gilbert et al

Conference (2014, May)

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