 Le virus de la diarrhée virale bovine et de la maladie des muqueuses (BVD/MD): diagnostic et comportement épidémiologique et biologiquePastoret, Paul-Pierre  ; ; et alReport (1998) Detailed reference viewed: 15 (2 ULg) Nucleotide sequence of the bovine viral diarrhoea virus Osloss strain: comparison with related viruses and identification of specific DNA probes in the 5' untranslated region; ; et al in Journal of General Virology (The) (1993), 74 The nucleotide sequence of the cytopathic Osloss isolate of bovine viral diarrhoea virus (BVDV) was deduced from overlapping cDNA clones and from PCR products. The Osloss genome is an RNA molecule of ... [more ▼] The nucleotide sequence of the cytopathic Osloss isolate of bovine viral diarrhoea virus (BVDV) was deduced from overlapping cDNA clones and from PCR products. The Osloss genome is an RNA molecule of positive polarity containing 12480 nucleotides and having the capacity to code for a polyprotein of 3975 amino acids. The presence of the previously described internal stop codon in this viral sequence was disproved after direct sequencing of the appropriate PCR-amplified fragment. Except for the previously reported insertion of a sequence coding for a ubiquitin-like protein, the viral genome shares great similarity with those of three other strains of the pestivirus genus. Computer-assisted sequence analyses and comparisons of known pestiviral genomic sequences led us to identify selected PCR primers in the 5' untranslated region. These primers were used successfully to amplify 18 distinct pestivirus isolates and potential DNA probes were noted from the deduced sequences. The possible use of a well conserved 26 base fragment as a diagnostic probe was confirmed in hybridization experiments. The 5' untranslated region was further studied and compared with those of other members of the Flaviviridae family, which includes the flaviviruses and the hepatitis C virus group. These sequence analyses support the possibility of discrimination amongst the closely related ruminant pestiviruses, border disease virus and BVDV. [less ▲] Detailed reference viewed: 9 (1 ULg)The cytopathogenic bovine viral diarrhoea virus Osloss strain: molecular characterization and expression of the proteins potentially involved in its cytopathogenicity; ; et al (1992) Detailed reference viewed: 11 (0 ULg)A "zinc finger-like" domain in the 54KDA protein of several pestiviruses; ; et al in Archives of Virology. Supplementum (1991), 3 Detailed reference viewed: 7 (0 ULg)The coding region for the 54-kDa protein of several pestiviruses lacks host insertions but reveals a "zinc finger-like" domain; ; et al in Virology (1990), 177(2), 812-5 We sequenced cDNAs, amplified by the polymerase chain reaction (PCR), which correspond to the carboxy-terminal portion of the 54-kDa protein of various cytopathic (cp) or noncytopathic (ncp) pestiviral ... [more ▼] We sequenced cDNAs, amplified by the polymerase chain reaction (PCR), which correspond to the carboxy-terminal portion of the 54-kDa protein of various cytopathic (cp) or noncytopathic (ncp) pestiviral strains. Except for the previously described insertions in two cp strains of the bovine viral diarrhea virus (BVDV), we did not find comparable insertions in this gene in eight pestiviral strains. The predicted amino acid sequences of this 54-kDa protein portion contain a conserved cysteine-rich stretch remarkably similar to a "zinc finger-type" binding domain found in many gene-regulatory proteins. Thus, this protein may be involved in the binding to viral RNA. [less ▲] Detailed reference viewed: 2 (0 ULg)Fish growth hormones; ; Rentier-Delrue, Françoise et alin Seminar on Use of Somatotropin in Livestock productio (1988) Detailed reference viewed: 8 (1 ULg) |
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