  Lack of risk of transmission of caprine arthritis-encephalitis virus (CAEV) after an appropriate embryo transfer procedure; ; Bouzar, Amel  et alin Theriogenology (2008) Detailed reference viewed: 7 (0 ULg)Kidding and embryo survival rate after traditional or direct transfer of frozen/thawed goat embryons; ; et al in Proceedings of the 15th International Congress on Animal Reproduction (2004) Detailed reference viewed: 2 (0 ULg)Effet de prétraitements agoniste et antagoniste de GnRH sur la production d’embryons chez la brebis et la chèvre; ; et al in Proceedings: 11e Rencontres autour des Recherches sur les Ruminants (3R) (2004) Detailed reference viewed: 11 (2 ULg)Amélioration des méthodes de cryopréservation et de transfert d’embryons chez les petits ruminants; ; et al in Proceedings: 8e Rencontres autour des Recherches sur les Ruminants (2001) ln small ruminants, the costs of embryo transfer is a main limiting factor to the use of this method. The use of ultra rapid techniques such as embryo vitrification and direct transfer may contribute to ... [more ▼] ln small ruminants, the costs of embryo transfer is a main limiting factor to the use of this method. The use of ultra rapid techniques such as embryo vitrification and direct transfer may contribute to reduce a part of the costs and increase the use of embryo transfer in sheep and goats. ln order to evaluate the efficiency of these techniques, two experiments were performed. ln a first experiment the viability of vitrified/thawed embryos was compared to results obtained after transfer of fresh embryos in ewes or frozen embryos (with slow freezing method) in goats. The pregnancy raté at term as weIl as embryo survival rate did not differ significantly according to embryo treatment (in ewes : 72% and 60% for fresh embryos vs 72% and 50% for vitrified embryos; in goats : 69% and 55% for frozen embryos vs 48% and 39 % for vitrified embryos). ln a second experiment, the possibility to transfer the vitrified embryos or frozen embryos directly after thawing (without cryoprotectant removal and evaluation of the morphological status of the embryos) was tested by comparison with the standard technique of transfer of vitrified or frozen/thawed embryos (removal of cryoprotectant and morphological evaluation). No significant effect of the transfer method was observed on the pregnancy rate at term and embryo survival rate (in ewes/vitrified embryos : 67% and 49% for traditional transfer vs 75% and 53% for direct transfer ; in goats/ vitrified embryos : 23% and 15% for traditional transfer vs 38% and 26% for direct transfer ; in goats/frozen embryos : 74% and 45% for traditional transfer vs 71% and 57% for direct transfer) [less ▲] Detailed reference viewed: 119 (1 ULg)Effet de prétraitements agoniste et antagoniste de GnRH sur la productîon d'embryons chez la brebis et la chèvre; ; et al in Proceedings: 8e Rencontres autour des Recherches sur les Ruminants (2001) ln the superovulated ewe, previous studies have shown that GnRH agonist and antagonist pre-treatment improve embryo production. Consequently, an experiment on this subject was continued in order to ... [more ▼] ln the superovulated ewe, previous studies have shown that GnRH agonist and antagonist pre-treatment improve embryo production. Consequently, an experiment on this subject was continued in order to simplify GnRH antagonist pretreatment in the ewe, and evaluate the efficiency of this technique in the superovulated goal. For the simplification of the pre-treatment, we compared the efficiency of the multiple low-dose (11' O.5mg/ day) antagonist regimen with a regimen of three injections of 1.5, 0.5, and O.5mgat 5 day intervals. The three injections regimen allowed a high ovulatory response, but a lower yield of transferable embryos (12 vs 8.3 ; P=O.08). GnRH agonist (Decapeptyl) and antagonist (Antarelix) pre-treatments were evaluated in superovulated goats. ln GnRH agonist treated goats (Decapeptyl1.8mg 22days before FSH) follicle number >3 mm was decreased (5.5 before vs 1.1 after treatment ; P<O.Ol) without an effect on the number of small follicles (2-3mm) and ovulatory response. Percentages of recovered and fertilised ova were significantly lower after Decapeptyl pre-treatment than without pre-treatment. Consequently a low number of transferable embryos per Decapeptyl treated goat was obtained (Decapeptyl + FSH: 2.2 vs FSH 4.7 ; P>O.l0). ln GnRH antagonist treated goats (Antarelix Il' 0.5mg / day before FSH) a decrease in follicle number >5 mm was observed as weIl as an increase in small follicle number (2-3mm) and ovulatory response after FSH treatment. However, this beneficial effect on ovulatory response was cancelled by an increase in the percentage of unfertilised ova and degenerated embryos. Consequently, in Antarelix treated goats the yield of embryo production was reduced (Antarelix +FSH 2.3 vs FSH 8.1 ; P<O.Ol). After in vitro fertilisation, the cleavage rate was also lower after Antarelix pre-treatment compared to the control group (84% vs 94%; P<O.Ol). However, this percentage was high (84%) as compared to the in vivo fertilisation rate (29%).A negative effect of Antarelix pre-treament on goat oocytequality is possible; nevertheless, transport and survival of spermatozoa in the genital tract are most probably affected in these conditions [less ▲] Detailed reference viewed: 164 (1 ULg)Four years of induction/synchronization of estrus in dairy goats: effect on the evolution of eCG binding rate in relation with the parameters of reproductionDrion, Pierre ; ; et alin Reproduction Nutrition Development (2001), 41(5), 401-412 Ninety-eight Alpine goats of two herds were followed over 4 years in a program of annual artificial insemination after estrus induction/synchronization, including progestagen administration (vaginal ... [more ▼] Ninety-eight Alpine goats of two herds were followed over 4 years in a program of annual artificial insemination after estrus induction/synchronization, including progestagen administration (vaginal sponge) followed by prostaglandin analog and equine chorionic gonadotrophin (eCG) 48 h before sponge removal. Goats were sampled every 4 hours from the 16th to the 56th following sponge removal, for determination of LH surge and tested for estrus by the presence of a buck. Seven days after AI, endoscopic examination of the ovaries was performed to determine the number of corpus lutea. Pregnancy diagnosis was performed at day 21-22 post AI by determination of plasma progesterone and at day 40-45 by ultrasonography. Parturition, number and sex of kids were recorded. All the goats were sampled before and after each treatment, for anti-eCG antibodies screening. Statistical analysis of the results clearly established a significant effect of the treatments on anti-eCG antibodies. Time of estrus and LH surge were significantly different between herd. The antibodies significantly delayed the time of coming out of estrus as well as the time of LH surge. Two antagonistic effects were evidenced: first, the delayed of time of estrus and time of LH surge in relation with the immune reaction to eCG; secondly, the ahead of time of estrus and time of LH surge during the years of treatment, identical to both herd. The antibodies negatively influenced the percentage of ovulating females as well as kidding rate. Finally, no effect of antibodies on prolificacy was found. [less ▲] Detailed reference viewed: 57 (9 ULg)Does annual repetition of estrous induction influence the fertility of goats after A.I. at a fixed time ?Drion, Pierre ; ; et alin Biotechnologie, Agronomie, Société et Environnement = Biotechnology, Agronomy, Society and Environment [=BASE] (2001), 5(1), 28-29 Detailed reference viewed: 31 (9 ULg)Effets de la répétition des traitements Progestagènes/PMSG chez la chèvre; ; et al (1998, April 30) Detailed reference viewed: 22 (2 ULg) Utilisation répétée des gonadotropines exogènes dans le contrôle de la reproduction: justifications et effets secondaires potentielsDrion, Pierre ; ; et alin Annales de Médecine Vétérinaire (1998), 142(2), 373-396 Gonadotropins of pituitary origin (follitropin -FSH-, lutropin -LH-, human menopausal gonadotropin -hMG) or trophoblastic origin (human chorionic gonadotropin - hCG- also called pregnant urine ... [more ▼] Gonadotropins of pituitary origin (follitropin -FSH-, lutropin -LH-, human menopausal gonadotropin -hMG) or trophoblastic origin (human chorionic gonadotropin - hCG- also called pregnant urine gonadotropin -pU-, equine chorionic gonadotropin -eCG- also called pregnant mare serum gonadotropin -PMSG-) are largely used to treat infertility or to better control sexuality in various species of mammals: bovine, canine, caprine, equine, feline, human,ovine, porcine, rabbit, ... This manuscript reports the discovery of these molecules, describes their biochemical characteristics and gives the main indications (e.g. oestrus induction and synchronization, ovulation, superovulation, fertility improvement, anoestrus treatment, receptivity induction) in animals and human. Litterature data reporting on effects and possible side effects of their repeated use are presented. Due to slight differences in amino acids sequences, carbohydrates contents and tridimentionnal structures, repeated use of exogeneous gonadotropins may induce immunological reactions leading to non specific reactions and lower biological response. A reflection concludes this revue, pointing out the positive influence of the philogenetic distance existing between the species origin of the gonadotropin (human or equine) and the treated one, on the intensity of the immune reaction. [less ▲] Detailed reference viewed: 139 (20 ULg) |
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