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See detailAnalysis of the diastereoisomers of alliin by HPLC
DETHIER, Bérénice ULg; Laloux, Morgan ULg; Hanon, Emilien et al

in Talanta (2012), 101

Garlic has been known for its therapeutic effects for centuries and is used worldwide as a functional food. The concentration of the active molecules could be enhanced by a better knowledge of their ... [more ▼]

Garlic has been known for its therapeutic effects for centuries and is used worldwide as a functional food. The concentration of the active molecules could be enhanced by a better knowledge of their biosynthesis. The precursor of these compounds, alliin (a sulfur amino-acid) has been obtained by chemical synthesis. However, this synthesis route also leads to a diastereoisomer as co-product. This work describes the development of an analytical method which allows the separation and quantification of the two diastereoisomers in order to determine in which proportion the natural form can be produced. The HPLC method which was optimized and validated by accuracy profile exploits an original stationary phase consisting of porous graphitic carbon (PGC). Furthermore, the developped method was used to separate the diastereoisomers of methiin, another garlic cysteine sulfoxide, and to analyse and aqueous extract of garlic. The ability to quantify the amount of natural alliin is valuable for further work on garlic molecules and their application for health protection. [less ▲]

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See detailDo root-emitted volatile organic compounds interact with wireworms?
Barsics, Fanny ULg; Gfeller, Aurélie ULg; Laloux, Morgan ULg et al

Scientific conference (2012, May 22)

Wireworms are the soil dwelling larvae of click beetles (Coleoptera: Elateridae). Their importance as crop pests increases since the efficient chemical means to control them cannot be considered anymore ... [more ▼]

Wireworms are the soil dwelling larvae of click beetles (Coleoptera: Elateridae). Their importance as crop pests increases since the efficient chemical means to control them cannot be considered anymore. Therefore, many integrated pest management strategies have been investigated in the past few years. Most of them rely on the understanding of the ecology of the click beetles during their whole life cycle. We focus our work on the chemical ecology of wireworms, more precisely on the root-emitted volatile organic compounds (VOCs) that might intervene in the food-searching process of the larvae by helping them to find a suitable host-plant or by acting as key factors in the belowground defence mechanism of the plant. Here, we present our first results of dual-choice orientation tests in olfactometric pipes. Wireworms (Agriotes sordidus Illiger) were submitted individually to a variety of olfactory baits ranging from entire barley roots (Hordeum vulgare L. var. Quench) to isolated VOCs identified as part of the emitting profile. The latter was described thanks to HS-SPME samplings and GC-MS analysis, for roots grown in the exact same conditions as for the olfactometric experimentations with entire roots. Most of the experimentations gave significant results. When confronted to volatiles emitted by entire roots, wireworms significantly orientated towards the bait (χ²-goodness-of-fit test, χ²=8, P-value=0.005). This result allowed us to follow up with the same device and to progressively vary the nature of the baits. Our protocol should be used for other plant-wireworm species combinations. Our results should be taken into account in varietal selection, in crop rotation, or in trapping systems aiming at the reduction of the populations of wireworms. [less ▲]

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See detailA new HPLC method to quantify alliin, the major sulfur compound in garlic
DETHIER, Bérénice ULg; Laloux, Morgan ULg; Hanon, Emilien et al

Poster (2012, February 10)

Garlic is recognized for centuries for its health benefits, mostly linked to its sulfur- components. Among the products extracted from the cloves, alliin retained our attention. Alliin was synthesized ... [more ▼]

Garlic is recognized for centuries for its health benefits, mostly linked to its sulfur- components. Among the products extracted from the cloves, alliin retained our attention. Alliin was synthesized. This process leads to two stereoisomers. Their HPLC separation was the aim of this work. The method described in the literature (amino column, detection at 210 nm) showed a resolution of 1,1 between the isomers. A second method was developed: the stationary phase was porous graphitic carbon and a water-ACN gradient was used for the elution. It provided a resolution of 3,2, was shorter and underwent the validation process. The method leads to satisfying results. The separation is excellent, and the validation criteria are fulfilled. This may be valuable for future research on garlic. [less ▲]

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See detailDo root-emitted volatile organic compounds attract wireworms?
Barsics, Fanny ULg; Latine, Rémi ULg; Gfeller, Aurélie ULg et al

in Communications in Agricultural and Applied Biological Sciences (2012), 77(3), 561-567

Wireworms are the soil dwelling larvae of click beetles (Coleoptera: Elateridae). Their importance as crop pests increases since the efficient chemical means to control them cannot be considered anymore ... [more ▼]

Wireworms are the soil dwelling larvae of click beetles (Coleoptera: Elateridae). Their importance as crop pests increases since the efficient chemical means to control them cannot be considered anymore. Therefore, many integrated pest management strategies have been investigated in the past few years. Most of them rely on the understanding of the ecology of the click beetles during their whole life cycle. We focus our work on the chemical ecology of wireworms, more precisely on the root-emitted volatile organic compounds (VOCs) that might intervene in the food-searching process of the larvae by helping them to find a suitable host-plant or by acting as key factors in the belowground defence mechanism of the plant. Here, we present our first results of dual-choice orientation tests in olfactometric pipes. Wireworms (Agriotes sordidus Illiger) were submitted individually to a variety of olfactory baits ranging from entire barley roots (Hordeum vulgare L. var. Quench) to isolated VOCs identified as part of the emitting profile. The latter was described thanks to HS-SPME samplings and GC-MS analysis, for roots grown in the exact same conditions as for the olfactometric experimentations with entire roots. Most of the experimentations gave significant results. When confronted to volatiles emitted by entire roots, wireworms significantly orientated towards the bait (χ²-goodness-of-fit test, χ²=8, P-value=0.005). This result allowed us to follow up with the same device and to progressively vary the nature of the baits. Our protocol should be used for other plant-wireworm species combinations. Our results should be taken into account in varietal selection, in crop rotation, or in trapping systems aiming at the reduction of the populations of wireworms. [less ▲]

Detailed reference viewed: 90 (37 ULg)
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See detailLes volatils racinaires de l’orge : un langage souterrain ?
Barsics, Fanny ULg; Gfeller, Aurélie ULg; Fauconnier, Marie-Laure ULg et al

Scientific conference (2011, October 13)

Cette présentation résume les avancées du projet Rhizovol après une année de travaux de recherche.

Detailed reference viewed: 73 (31 ULg)